FastQCFastQC Report
Fri 27 May 2016
SRR2032142_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2032142_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30225663
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT3921521.2974140550696935No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC2925040.9677339418493482No Hit
CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT938250.31041502712446706No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA872930.2888042522011841No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT820760.2715440849055983No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC819620.27116692196296904No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC776010.2567387851839677No Hit
CCGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCT762800.25236832687507965No Hit
CTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA717010.2372189486794715No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC686240.22703885767534696No Hit
CCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCC631550.2089449617697385No Hit
CGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTC589480.1950263258079732No Hit
CCCGTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGA532000.17600937322698265No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC512550.16957444407422922No Hit
GTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCAT510080.16875725769853253No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT493610.16330824571159944No Hit
CTCACTTCTATGAAATAAAACTCCAGCAATACTAACTATAATCTCACTGA462460.1530024337265985No Hit
GCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGT461120.15255910184666585No Hit
CCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGC446960.14787434108558678No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT443990.14689173236663164No Hit
CTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCA443850.14684541411051924No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG441990.14623004299359788No Hit
CATTATTCTTATAGGAATTAACATTATTATTACAGGTATATACTCAATAT434130.14362960375757514No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC431360.14271316397592337No Hit
GCCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCG417420.138101189045878No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT410320.13575219177160813No Hit
CTCAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTAC407230.13472988169027095No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC405420.13413105280767537No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA388010.12837104681541642No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG387620.12824201738767482No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCC386150.12775567569849502No Hit
ATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTG381950.12636612801512412No Hit
CCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAATACACGACAGC378990.12538682774303414No Hit
CTGAAATTTGTGGATCTAACCATAGCTTTATGCCCATTGTCCTAGAAATG375290.12416270240292165No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA374070.12375907188537105No Hit
CCCAAATAAATCTGTACTCAGAAAAAGCAAATCCATATGAATGCGGATTC373060.123424918751989No Hit
CTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGA347780.11506116507684215No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA340700.11271878469630262No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA336030.11117374001026875No Hit
CTCCCTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAA331510.10967832202721244No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG324460.1073458669872684No Hit
CCCCCTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATT319070.1055626141269424No Hit
GGCTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAA315070.10423923538087486No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT313820.10382567952272874No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT302930.10022278088655986No Hit
CTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCAATTACAT302310.10001765718091941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA626950.063.032961
CCAAACG712350.055.59898
CCGAAAC711300.055.593842
CGAAACC791650.049.860783
CAAACGA843150.047.024919
GTCCGTA12700.044.179991
AAACCAA920900.043.2761465
CGCCCAT13350.043.0974351
GAAACCA934050.042.5600554
GATATAT232500.041.229255
ACTCGTC580850.041.07042342-43
TCCGCTC26100.040.751762
CCAGATA211450.040.735062
TCGTCTA594100.040.1213544-45
CTAACGA578550.039.7618494-95
CGCTCGT18650.039.263521
ACCAAAC1030500.038.8612867
CAGATAT229500.038.1929743
CTCCGCT35400.038.1471751
CGCCTAT15650.037.978951
Produced by FastQC (version 0.11.5)