##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2032142_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30225663 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52878098984959 34.0 31.0 34.0 31.0 34.0 2 32.665439629893314 34.0 31.0 34.0 31.0 34.0 3 32.788452018405685 34.0 31.0 34.0 31.0 34.0 4 36.24060481320129 37.0 37.0 37.0 35.0 37.0 5 36.2468073901307 37.0 37.0 37.0 35.0 37.0 6 36.1686334887013 37.0 37.0 37.0 35.0 37.0 7 36.08343426577607 37.0 37.0 37.0 35.0 37.0 8 36.04286370823363 37.0 37.0 37.0 35.0 37.0 9 37.85309734975871 39.0 38.0 39.0 35.0 39.0 10-11 37.94850136124392 39.0 38.0 39.0 35.0 39.0 12-13 37.867069781066505 39.0 38.0 39.0 35.0 39.0 14-15 39.32938339516324 41.0 39.0 41.0 36.0 41.0 16-17 39.29811590898767 41.0 39.0 41.0 36.0 41.0 18-19 39.30405935181636 41.0 39.0 41.0 36.0 41.0 20-21 39.28588744273368 41.0 39.0 41.0 36.0 41.0 22-23 39.24529046724302 41.0 39.0 41.0 36.0 41.0 24-25 39.22899319032307 41.0 39.0 41.0 36.0 41.0 26-27 39.179238880549946 41.0 39.0 41.0 36.0 41.0 28-29 39.14137425207183 40.5 39.0 41.0 36.0 41.0 30-31 39.06470408936936 40.0 39.0 41.0 36.0 41.0 32-33 39.006850039980925 40.0 39.0 41.0 35.0 41.0 34-35 38.92062278336128 40.0 38.0 41.0 35.0 41.0 36-37 38.81423082431641 40.0 38.0 41.0 35.0 41.0 38-39 38.50815252588504 40.0 38.0 41.0 34.5 41.0 40-41 38.255967619304165 40.0 38.0 41.0 34.0 41.0 42-43 38.2331865143868 40.0 38.0 41.0 34.0 41.0 44-45 38.24891535050861 40.0 38.0 41.0 34.0 41.0 46-47 38.17598452679103 40.0 38.0 41.0 33.5 41.0 48-49 38.25347791378472 40.0 38.0 41.0 33.5 41.0 50-51 38.54477789287864 40.0 38.0 41.0 34.0 41.0 52-53 38.489702574927804 40.0 38.0 41.0 34.0 41.0 54-55 38.35504622346911 40.0 38.0 41.0 34.0 41.0 56-57 38.22295439805572 40.0 37.5 41.0 34.0 41.0 58-59 38.04615667156747 40.0 37.0 41.0 33.5 41.0 60-61 37.852938461598015 39.5 37.0 41.0 33.0 41.0 62-63 37.56927935708143 39.0 36.0 41.0 33.0 41.0 64-65 37.21112539367623 39.0 35.5 41.0 32.5 41.0 66-67 36.88681970350824 38.5 35.0 40.0 32.0 41.0 68-69 36.464067438322196 37.0 35.0 40.0 32.0 41.0 70-71 36.12503333673773 37.0 35.0 39.0 31.0 41.0 72-73 35.803092805606944 36.5 35.0 39.0 31.0 41.0 74-75 35.46938781789501 36.0 35.0 39.0 31.0 41.0 76-77 35.347785307471995 36.0 35.0 38.5 32.0 40.0 78-79 34.997633765717566 35.0 35.0 37.0 31.0 39.5 80-81 34.64528822742449 35.0 34.0 37.0 31.0 39.0 82-83 34.35616474318529 35.0 34.0 36.0 31.0 39.0 84-85 34.12751558170949 35.0 34.0 36.0 31.0 37.0 86-87 33.87064555043838 35.0 34.0 36.0 31.0 37.0 88-89 33.737641735104376 35.0 34.0 35.5 31.0 37.0 90-91 33.629736889477 35.0 34.0 35.0 31.0 36.0 92-93 33.507721170582755 35.0 34.0 35.0 31.0 36.0 94-95 33.381969338439326 35.0 34.0 35.0 31.0 36.0 96-97 33.27149723729799 35.0 34.0 35.0 31.0 36.0 98-99 33.17037039022105 35.0 34.0 35.0 31.0 36.0 100-101 32.40545067613571 34.5 33.0 35.0 28.5 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26330859608559365 1101 2 0.30376794169757204 1101 3 0.2418773479402887 1101 4 0.1834482982122907 1101 5 0.15349433560938763 1101 6 0.15894980310591933 1101 7 0.17268608450894618 1101 8 0.12874828586907938 1101 9 0.17811268668548053 1101 10-11 0.17108546128091007 1101 12-13 0.18921689878156656 1101 14-15 0.2708747916929184 1101 16-17 0.2390227499194424 1101 18-19 0.2971864633084067 1101 20-21 0.30822061123522815 1101 22-23 0.26739598848224944 1101 24-25 0.3219977255011486 1101 26-27 0.29740092998502377 1101 28-29 0.30593644051521096 1101 30-31 0.29244908425405214 1101 32-33 0.27932676491411 1101 34-35 0.2950498164637807 1101 36-37 0.3550696107316895 1101 38-39 0.3333640471016963 1101 40-41 0.31454593200992065 1101 42-43 0.26241430502380325 1101 44-45 0.23726773430134074 1101 46-47 0.24865171327759583 1101 48-49 0.2789629738589099 1101 50-51 0.2789662878158765 1101 52-53 0.27376311370372974 1101 54-55 0.2750798263108294 1101 56-57 0.30633630517814225 1101 58-59 0.2900855050434501 1101 60-61 0.2570342248399484 1101 62-63 0.26242265286084887 1101 64-65 0.26851892165042557 1101 66-67 0.2600100219100199 1101 68-69 0.19318861094576079 1101 70-71 0.24170845012857 1101 72-73 0.22294202235772076 1101 74-75 0.2615366387174589 1101 76-77 0.30581408131020993 1101 78-79 0.3312589073271326 1101 80-81 0.3498014631091806 1101 82-83 0.3419865371300972 1101 84-85 0.3152759129646512 1101 86-87 0.323432530901151 1101 88-89 0.30628807336375274 1101 90-91 0.31796253855519296 1101 92-93 0.3126449546054886 1101 94-95 0.3208040920653801 1101 96-97 0.33543412428244324 1101 98-99 0.34881985515384883 1101 100-101 0.3744333630887837 1102 1 0.14540227421381502 1102 2 0.19847138036847412 1102 3 0.14682420345562974 1102 4 0.12140432171354831 1102 5 0.13299215785801977 1102 6 0.1563498074049221 1102 7 0.14194277988563186 1102 8 0.11996325733186097 1102 9 0.14797533746021685 1102 10-11 0.15781880488130184 1102 12-13 0.1767193583200779 1102 14-15 0.2545064111908957 1102 16-17 0.25411410710442794 1102 18-19 0.2746149587718705 1102 20-21 0.2595261104225486 1102 22-23 0.2683102142834528 1102 24-25 0.29059026651464137 1102 26-27 0.28476632365639887 1102 28-29 0.30243773243373084 1102 30-31 0.3030903838190113 1102 32-33 0.2800192478163339 1102 34-35 0.31155341236061673 1102 36-37 0.31132870693049597 1102 38-39 0.3380421876443691 1102 40-41 0.34444642701137695 1102 42-43 0.2806564420105033 1102 44-45 0.23413690344329297 1102 46-47 0.20911789727458086 1102 48-49 0.23826205529650224 1102 50-51 0.25043194011532677 1102 52-53 0.2684575165421563 1102 54-55 0.23444284307387875 1102 56-57 0.24944404155970545 1102 58-59 0.28551554180387484 1102 60-61 0.2677001810914774 1102 62-63 0.2556297435017427 1102 64-65 0.2530818908319148 1102 66-67 0.25090311924468267 1102 68-69 0.2691933901973158 1102 70-71 0.2792757262786836 1102 72-73 0.24550451766037185 1102 74-75 0.2547873572825665 1102 76-77 0.29385207128057544 1102 78-79 0.3307343966086407 1102 80-81 0.3367359679093127 1102 82-83 0.3171966664190293 1102 84-85 0.2919882881056708 1102 86-87 0.30435406666027376 1102 88-89 0.28915738138360325 1102 90-91 0.31267513545365944 1102 92-93 0.309997312099334 1102 94-95 0.3046345755175537 1102 96-97 0.31023897000055456 1102 98-99 0.29566686933144126 1102 100-101 0.3719831340823987 1103 1 0.2635333419487367 1103 2 0.30053395765850155 1103 3 0.22238289935232558 1103 4 0.15159407478019915 1103 5 0.1361024101796815 1103 6 0.11866935290826319 1103 7 0.11150228507050741 1103 8 0.09526509530689964 1103 9 0.1312626110332289 1103 10-11 0.1473340658474882 1103 12-13 0.17390437985647367 1103 14-15 0.22151286432921324 1103 16-17 0.21905044264325824 1103 18-19 0.21885719257806358 1103 20-21 0.2040193180724259 1103 22-23 0.21668642832246832 1103 24-25 0.24322970997184257 1103 26-27 0.26166342401841547 1103 28-29 0.25284765065340764 1103 30-31 0.23828123599579243 1103 32-33 0.26652068041942556 1103 34-35 0.3142342629973953 1103 36-37 0.29113233339415245 1103 38-39 0.3222896182481776 1103 40-41 0.309039637620927 1103 42-43 0.24108630073730097 1103 44-45 0.2018921143244512 1103 46-47 0.21241518352816513 1103 48-49 0.23720802967548593 1103 50-51 0.2449663390962229 1103 52-53 0.25904513865163636 1103 54-55 0.2692201252448143 1103 56-57 0.2598910187014454 1103 58-59 0.25389134048529627 1103 60-61 0.26703700613955306 1103 62-63 0.26717470974080015 1103 64-65 0.24470004588437888 1103 66-67 0.24121873272081018 1103 68-69 0.26827074671670204 1103 70-71 0.26645563355051394 1103 72-73 0.2269964442003456 1103 74-75 0.21931663726415707 1103 76-77 0.23212179418450773 1103 78-79 0.2773172433868396 1103 80-81 0.2601427331061572 1103 82-83 0.25778063859910105 1103 84-85 0.27524013698545957 1103 86-87 0.2509309439494487 1103 88-89 0.2737187509429546 1103 90-91 0.2597592870154699 1103 92-93 0.2538964488276676 1103 94-95 0.26287156612564644 1103 96-97 0.29239323362871517 1103 98-99 0.3208934843177502 1103 100-101 0.3674705717337261 1104 1 0.21945867378963868 1104 2 0.2387913508310362 1104 3 0.1891631323979368 1104 4 0.11910399538075467 1104 5 0.08758236067829728 1104 6 0.10120545924613111 1104 7 0.11699618958908786 1104 8 0.10915083238807455 1104 9 0.11928330310003332 1104 10-11 0.1306797984208572 1104 12-13 0.11199687525274271 1104 14-15 0.1965519843864385 1104 16-17 0.17762651443562305 1104 18-19 0.2042199689328683 1104 20-21 0.23086042586119504 1104 22-23 0.20252334370894687 1104 24-25 0.2547449805152411 1104 26-27 0.26845395813692363 1104 28-29 0.22608711121393554 1104 30-31 0.24381874007204374 1104 32-33 0.2283486233740959 1104 34-35 0.2637512511786255 1104 36-37 0.21963620376475745 1104 38-39 0.20591589503897723 1104 40-41 0.3301269524259922 1104 42-43 0.26544537438427085 1104 44-45 0.19101186702800987 1104 46-47 0.19975541612816983 1104 48-49 0.18349424983483686 1104 50-51 0.21825131516251162 1104 52-53 0.2258813021397117 1104 54-55 0.22352389300682063 1104 56-57 0.2081175069310106 1104 58-59 0.20205217304619083 1104 60-61 0.19585568394496988 1104 62-63 0.1994796686821374 1104 64-65 0.20468297086889464 1104 66-67 0.20220756816421925 1104 68-69 0.19211322010458076 1104 70-71 0.22126244737437162 1104 72-73 0.24324214743852934 1104 74-75 0.23270931830924724 1104 76-77 0.26084446921246496 1104 78-79 0.26307876017382625 1104 80-81 0.2145705994961844 1104 82-83 0.2111723034604296 1104 84-85 0.18059659420634944 1104 86-87 0.2390967246290714 1104 88-89 0.2282569625482722 1104 90-91 0.22350415565005477 1104 92-93 0.20757838020752217 1104 94-95 0.1797236101983799 1104 96-97 0.16397926317517175 1104 98-99 0.2306951011882532 1104 100-101 0.3112366804963287 1105 1 0.199040738438228 1105 2 0.24916277138729725 1105 3 0.18046056181513137 1105 4 0.11719005304590269 1105 5 0.1118481460941112 1105 6 0.1115630224958366 1105 7 0.0707954994722968 1105 8 0.09343684606341895 1105 9 0.10334605661973484 1105 10-11 0.11018278452689145 1105 12-13 0.08641376276985113 1105 14-15 0.1405424875103165 1105 16-17 0.1518245674223806 1105 18-19 0.15503007892106524 1105 20-21 0.14198463546740925 1105 22-23 0.1650563977896624 1105 24-25 0.1814048526286527 1105 26-27 0.15363808026142323 1105 28-29 0.11793544681961521 1105 30-31 0.16367370626806377 1105 32-33 0.1478650813442215 1105 34-35 0.17504188919702557 1105 36-37 0.1491589832916702 1105 38-39 0.20063754863609518 1105 40-41 0.2824108988431533 1105 42-43 0.21252370319704283 1105 44-45 0.19897250040810377 1105 46-47 0.17872621248752552 1105 48-49 0.1640435566617242 1105 50-51 0.1509826920488493 1105 52-53 0.16817432273949606 1105 54-55 0.1342686880989703 1105 56-57 0.08965554951935673 1105 58-59 0.1719718300523141 1105 60-61 0.14874834262644043 1105 62-63 0.1540474205800848 1105 64-65 0.1489677634946247 1105 66-67 0.1344479681628883 1105 68-69 0.14737721366678613 1105 70-71 0.13465544987212752 1105 72-73 0.1652543307130756 1105 74-75 0.14985444748440102 1105 76-77 0.12916768543907153 1105 78-79 0.19468644029193882 1105 80-81 0.22723885821746848 1105 82-83 0.20443946400649793 1105 84-85 0.22985393512946217 1105 86-87 0.2446879099718302 1105 88-89 0.260049463909354 1105 90-91 0.22840746371725373 1105 92-93 0.22417435082552828 1105 94-95 0.24194703568623765 1105 96-97 0.21912528462414116 1105 98-99 0.22145275494193584 1105 100-101 0.2887897489774218 1106 1 0.17204623679799624 1106 2 0.23175024788756815 1106 3 0.13671237404727776 1106 4 0.08104403310741759 1106 5 0.08161377399037661 1106 6 0.08255043092092507 1106 7 0.09942102832115296 1106 8 0.07729760344609105 1106 9 0.08049218450571516 1106 10-11 0.08852001144457944 1106 12-13 0.10275569562955411 1106 14-15 0.11908461952198479 1106 16-17 0.13818778144982957 1106 18-19 0.15759511816342808 1106 20-21 0.16097039639228683 1106 22-23 0.14058053980324559 1106 24-25 0.19471394388077812 1106 26-27 0.18390556084051468 1106 28-29 0.17538398218440676 1106 30-31 0.14374930844972056 1106 32-33 0.15814834838735692 1106 34-35 0.17788958867557625 1106 36-37 0.19843462936107414 1106 38-39 0.23286006088949307 1106 40-41 0.287474233554434 1106 42-43 0.2382749633321808 1106 44-45 0.2227700180997445 1106 46-47 0.2089813927166091 1106 48-49 0.20916441013351061 1106 50-51 0.15191476549472327 1106 52-53 0.18077337773531355 1106 54-55 0.22025489844531165 1106 56-57 0.19609348852244324 1106 58-59 0.16811655576877627 1106 60-61 0.1611155918764382 1106 62-63 0.1642485719061284 1106 64-65 0.15952146354524643 1106 66-67 0.17618104350530928 1106 68-69 0.19706791344497532 1106 70-71 0.23551744316695533 1106 72-73 0.13521068141574943 1106 74-75 0.1726548941332524 1106 76-77 0.18626034493543386 1106 78-79 0.22099218896487827 1106 80-81 0.22008704995458572 1106 82-83 0.1967091487310313 1106 84-85 0.21007940028542293 1106 86-87 0.21378717424304483 1106 88-89 0.2107054576615468 1106 90-91 0.2170376997808745 1106 92-93 0.23584337577884185 1106 94-95 0.2329388141695361 1106 96-97 0.2511003164708967 1106 98-99 0.2695374926440479 1106 100-101 0.38458615402743845 1107 1 0.16360082236562334 1107 2 0.20274834919783302 1107 3 0.10919053003726731 1107 4 0.06677582452554276 1107 5 0.055292744062676036 1107 6 0.02706081940571181 1107 7 0.05970381307051298 1107 8 0.04579774779168133 1107 9 0.03325288537639892 1107 10-11 0.06998527176243385 1107 12-13 0.05715274690300021 1107 14-15 0.07039798601451963 1107 16-17 0.08362330036444376 1107 18-19 0.07884557571292561 1107 20-21 0.10320121936215543 1107 22-23 0.08931853989607674 1107 24-25 0.15071660915388918 1107 26-27 0.13481145367951797 1107 28-29 0.11276271918923442 1107 30-31 0.13866395499467643 1107 32-33 0.1261744187419751 1107 34-35 0.12357741253442356 1107 36-37 0.1125861385108422 1107 38-39 0.03515512069078852 1107 40-41 0.23226887134062224 1107 42-43 0.19350364914595275 1107 44-45 0.2349475869022939 1107 46-47 0.19859604541082376 1107 48-49 0.1816384475168391 1107 50-51 0.17017120806291075 1107 52-53 0.16217967415028767 1107 54-55 0.09491768435662351 1107 56-57 0.14495912089260088 1107 58-59 0.18368137091127323 1107 60-61 0.18537160664192243 1107 62-63 0.14438236228892265 1107 64-65 0.16415035429051272 1107 66-67 0.18442791875759923 1107 68-69 0.16947797157893518 1107 70-71 0.17434758641296355 1107 72-73 0.1432198389375472 1107 74-75 0.1494635063486669 1107 76-77 0.1283825546307682 1107 78-79 0.18725460113865466 1107 80-81 0.18343044682445964 1107 82-83 0.19610145853881988 1107 84-85 0.16402111660090668 1107 86-87 0.19222748267086587 1107 88-89 0.2184029413887032 1107 90-91 0.2072420284067249 1107 92-93 0.2085077790938925 1107 94-95 0.20632364915273627 1107 96-97 0.19068507845284444 1107 98-99 0.2547973243598136 1107 100-101 0.32549504325673695 1108 1 0.15833913674867972 1108 2 0.1970316235561853 1108 3 0.10234743728612727 1108 4 0.06253282003865479 1108 5 0.06307999818666588 1108 6 0.06185788513250401 1108 7 0.053476044960277136 1108 8 0.057585757294532414 1108 9 0.056435478903587466 1108 10-11 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-0.03873724966994985 2214 46-47 0.0380651250403119 2214 48-49 -0.061938614689864835 2214 50-51 -0.1321648802339226 2214 52-53 -0.11891794609234552 2214 54-55 -0.11102271597532365 2214 56-57 -0.1305944416671494 2214 58-59 -0.12510418365120302 2214 60-61 -0.09759373236143887 2214 62-63 -0.060046919786785224 2214 64-65 -0.1220830967336255 2214 66-67 -0.08958857436824985 2214 68-69 -0.047779443494725626 2214 70-71 -0.007463725080611994 2214 72-73 -0.013937052361718827 2214 74-75 -0.014334408133926502 2214 76-77 -0.07463343750222862 2214 78-79 -0.07592557990347615 2214 80-81 -0.0819950508449736 2214 82-83 -0.07819977224401242 2214 84-85 -0.07336902687209346 2214 86-87 -0.021381175670420305 2214 88-89 -0.018337053514230206 2214 90-91 -0.03668176164801196 2214 92-93 -0.018415887286266752 2214 94-95 0.011387704651298236 2214 96-97 0.016853863274405967 2214 98-99 -0.0013140715590225227 2214 100-101 0.20397452585791598 2215 1 -0.07455801301090048 2215 2 -0.07754568816716301 2215 3 -0.1508757194316459 2215 4 -0.1208718216309066 2215 5 -0.15078816611568868 2215 6 -0.26003294992225534 2215 7 -0.2292935363651054 2215 8 -0.18788353689244985 2215 9 -0.22268046115776485 2215 10-11 -0.22971003058565032 2215 12-13 -0.23568265470080263 2215 14-15 -0.3207690565469363 2215 16-17 -0.3321123476150234 2215 18-19 -0.3240705759362328 2215 20-21 -0.3641537449151997 2215 22-23 -0.33999081295330313 2215 24-25 -0.3391938676325452 2215 26-27 -0.3178702165526417 2215 28-29 -0.31752229196997916 2215 30-31 -0.3061416511298418 2215 32-33 -0.3215493984529232 2215 34-35 -0.2818814426331997 2215 36-37 -0.25514089446159716 2215 38-39 -0.33528005011736184 2215 40-41 -0.41819249042231377 2215 42-43 -0.24637606939975143 2215 44-45 -0.17501213924774817 2215 46-47 -0.08325076254300257 2215 48-49 -0.1264758777037116 2215 50-51 -0.30192448164254415 2215 52-53 -0.2947249025361245 2215 54-55 -0.32212807181053904 2215 56-57 -0.30028819257614003 2215 58-59 -0.28235726438920494 2215 60-61 -0.3701730839069981 2215 62-63 -0.3310685062426728 2215 64-65 -0.3117262728130399 2215 66-67 -0.32101839920207453 2215 68-69 -0.3027702718579022 2215 70-71 -0.22776538059665796 2215 72-73 -0.2565115195726193 2215 74-75 -0.2565649260366598 2215 76-77 -0.30904064804163056 2215 78-79 -0.3205252435263972 2215 80-81 -0.2716383968297009 2215 82-83 -0.2558240112233179 2215 84-85 -0.2666642387215674 2215 86-87 -0.25262821008390546 2215 88-89 -0.232600084542959 2215 90-91 -0.24712749258925726 2215 92-93 -0.28191313583954525 2215 94-95 -0.2730387317829184 2215 96-97 -0.21427924777647434 2215 98-99 -0.23476985109969917 2215 100-101 0.0020345919974786852 2216 1 -0.26633045712659964 2216 2 -0.22647982573799652 2216 3 -0.3286736442113707 2216 4 -0.25158571786629125 2216 5 -0.22195557171502145 2216 6 -0.36027696647909124 2216 7 -0.37371519810534437 2216 8 -0.3209485290908205 2216 9 -0.3863015452753018 2216 10-11 -0.41148634054997046 2216 12-13 -0.46402872709766285 2216 14-15 -0.6724296967604886 2216 16-17 -0.6195053173530951 2216 18-19 -0.6470428841751428 2216 20-21 -0.5929248516868597 2216 22-23 -0.6252107714717283 2216 24-25 -0.6187956249471114 2216 26-27 -0.639143827231365 2216 28-29 -0.6191937880999845 2216 30-31 -0.6185745922023145 2216 32-33 -0.6983335075815518 2216 34-35 -0.7672156449070613 2216 36-37 -0.6710371557502768 2216 38-39 -0.6511782869420841 2216 40-41 -0.7604563455441777 2216 42-43 -0.6594646487831994 2216 44-45 -0.45672840297453376 2216 46-47 -0.3701913712008462 2216 48-49 -0.43116516373826386 2216 50-51 -0.6905397119568235 2216 52-53 -0.7284556189730864 2216 54-55 -0.805610030476771 2216 56-57 -0.7294081042844809 2216 58-59 -0.7431144174746152 2216 60-61 -0.7222884670643381 2216 62-63 -0.7650225030979669 2216 64-65 -0.7845974957355395 2216 66-67 -0.8111137283209686 2216 68-69 -0.7919298604856877 2216 70-71 -0.7426674461522254 2216 72-73 -0.7525243600376754 2216 74-75 -0.7385983833999887 2216 76-77 -0.7105896364262918 2216 78-79 -0.7858571407721797 2216 80-81 -0.5965926317878356 2216 82-83 -0.6326465409967739 2216 84-85 -0.7175733734753393 2216 86-87 -0.5883322551828769 2216 88-89 -0.5882806544783392 2216 90-91 -0.7144799116766265 2216 92-93 -0.6580909571127336 2216 94-95 -0.7092251769672231 2216 96-97 -0.6800040838808741 2216 98-99 -0.690900947831615 2216 100-101 -0.4039319956511349 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1651.0 3 650.0 4 789.0 5 1092.0 6 2080.0 7 3857.0 8 6469.0 9 8231.0 10 10446.0 11 14020.0 12 16866.0 13 17479.0 14 18682.0 15 21140.0 16 22841.0 17 27722.0 18 26177.0 19 28051.0 20 31396.0 21 35581.0 22 42702.0 23 53725.0 24 68450.0 25 89398.0 26 116602.0 27 149924.0 28 195520.0 29 248890.0 30 323486.0 31 424639.0 32 576136.0 33 820539.0 34 1225292.0 35 1901297.0 36 3285982.0 37 6563232.0 38 1.0344504E7 39 3478100.0 40 22025.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.956861460647065 9.820801977693826 5.557370408819736 56.66496615283937 2 19.499400786562134 12.431365388453845 40.935692055871975 27.133541769112046 3 21.80366730086285 15.85440822257563 22.142091639147832 40.19983283741369 4 23.219264029214788 25.69382404581698 20.954057943048003 30.132853981920228 5 22.26208042478788 33.42885007526741 24.23682509232311 20.0722444076216 6 18.157573701631485 37.06698769398231 25.419310055102834 19.356128549283365 7 14.747767704112169 27.947966653934515 40.19146736611452 17.112798275838795 8 16.128870357616307 24.504835510142488 33.489439090219456 25.87685504202174 9 15.344354894713144 24.211703809441666 36.883908882329564 23.56003241351563 10-11 19.165118043881403 35.33024862695066 25.971640658108193 19.53299267105975 12-13 20.345567716619186 28.7532445876944 28.440859298995363 22.460328396691054 14-15 20.39779165720514 31.556268081914556 26.666109300101716 21.37983096077859 16-17 20.880756960819994 28.859850022461874 27.73464215065349 22.524750866064643 18-19 19.845223907908984 29.305229797606092 27.687273559557653 23.16227273492727 20-21 20.615068063188556 28.826581901611227 26.487078215620947 24.071271819579277 22-23 20.375314844210365 30.42238147100363 27.026765302054745 22.175538382731258 24-25 19.947541035674146 30.891727953157478 26.57852980576354 22.582201205404832 26-27 19.85918074720515 32.16258116951998 26.95512831700077 21.023109766274096 28-29 20.054143001396508 30.27884171816297 26.83011643157406 22.836898848866465 30-31 18.644305444997837 29.888079749774526 28.52741588735884 22.940198917868795 32-33 19.614778822750825 28.622173865192508 29.86524497793338 21.897802334123288 34-35 19.58423520520693 30.841822848029572 27.07968171793097 22.494260228832523 36-37 20.17707983839892 30.45969479931923 27.46393227685171 21.89929308543014 38-39 19.627665393275034 30.27635830945112 26.891320451123878 23.204655846149972 40-41 19.84386831842303 30.562590851089517 26.169955846079507 23.42358498440795 42-43 19.11697846901316 30.73645895345829 27.489989041942003 22.656573535586546 44-45 19.049544406181337 28.79041112829206 27.555321574837276 24.604722890689327 46-47 19.484645726725685 30.011653282451555 27.519375040494392 22.98432595032837 48-49 18.775218800620415 29.073388455194234 28.22137565624966 23.930017087935703 50-51 18.396812463923386 29.713852193453345 28.574007274561236 23.315328068062033 52-53 20.707325636102738 29.136931423296847 27.701618312203593 22.454124628396823 54-55 19.715668468639077 29.225547224783664 27.209852888905147 23.848931417672116 56-57 21.13813873305455 28.471856192000423 26.824176697958247 23.565828376986783 58-59 19.22525525695428 31.38747710361118 27.275083703344517 22.112183936090027 60-61 18.48623459535729 32.433810804526544 26.544373205413628 22.535581394702536 62-63 19.195440585600913 31.07116588051282 27.96876466056827 21.764628873318 64-65 20.792223847829646 30.076102182401403 26.686061136301024 22.44561283346793 66-67 21.214281732325954 30.26238184479682 26.704360425539868 21.818975997337358 68-69 19.865402766895148 30.178687798729587 26.16432227756845 23.791587156806816 70-71 19.781343421142505 31.386867037266235 26.126802989744547 22.704986551846716 72-73 18.653341677043368 30.91423476457914 27.249478275496212 23.18294528288128 74-75 18.95012703159234 29.36637021550508 28.65492746005102 23.028575292851556 76-77 18.119050125592057 29.251339541616954 28.52897927249708 24.100631060293914 78-79 20.15842598187956 31.54757479524918 26.7134504808937 21.580548741977555 80-81 19.385038297681152 30.639971193882836 26.92044930436195 23.05454120407406 82-83 19.873273129139456 30.951421917588544 25.834630090244755 23.34067486302725 84-85 19.285566704873837 30.860663812239736 25.879946330200944 23.973823152685483 86-87 19.687237887958904 30.834963076361966 27.162713001392465 22.315086034286672 88-89 18.349568390054756 31.000147146428997 27.432148213600488 23.21813624991576 90-91 18.624595154369043 32.79553093976742 27.038591483049384 21.541282422814152 92-93 20.055328693649997 30.75823727769949 27.892226233998397 21.29420779465212 94-95 18.54134501154732 29.898607114058702 28.20452059981588 23.3555272745781 96-97 18.419259005033645 31.208053646859696 28.861736468252815 21.510950879853848 98-99 19.216849328155043 30.192571430698294 27.678856000492964 22.911723240653703 100-101 20.135574648872662 31.11974219057922 26.718562351101195 22.026120809446923 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 39.0 1 66.5 2 98.0 3 99.0 4 101.0 5 115.0 6 125.0 7 128.0 8 131.5 9 137.0 10 164.0 11 215.0 12 274.5 13 377.5 14 560.0 15 816.0 16 1349.0 17 2294.5 18 3723.0 19 5839.5 20 11507.0 21 19730.0 22 30537.0 23 59511.0 24 122303.5 25 168095.0 26 163926.5 27 207466.0 28 311609.0 29 414429.5 30 497136.5 31 575715.5 32 660816.5 33 743404.0 34 857734.5 35 1034933.0 36 1216889.5 37 1378660.5 38 1637233.5 39 1590968.5 40 1392226.0 41 1514140.5 42 1336027.5 43 1013358.0 44 973068.5 45 976484.0 46 934968.5 47 917779.5 48 905174.0 49 929477.0 50 896504.0 51 748424.0 52 676161.0 53 643234.0 54 631087.0 55 601864.0 56 551617.5 57 479521.0 58 396173.5 59 361018.0 60 322667.5 61 247529.5 62 193490.0 63 152601.5 64 116282.5 65 92334.5 66 67759.5 67 46359.0 68 27797.0 69 19185.5 70 25745.0 71 50151.0 72 48389.0 73 102065.5 74 95714.0 75 10574.5 76 4264.0 77 2968.0 78 1121.5 79 583.5 80 240.0 81 115.5 82 72.5 83 39.5 84 23.0 85 9.0 86 10.5 87 10.0 88 6.5 89 2.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14103578141528278 2 6.815400542247824E-4 3 0.0 4 0.010451383647068387 5 0.013515005444214738 6 0.014395052310349652 7 0.009816161848955968 8 0.0 9 0.0 10-11 0.0013895476833709157 12-13 0.0010851705717753817 14-15 6.368760215450031E-4 16-17 1.1579564028090963E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.400234330674567E-4 26-27 3.589664848708199E-4 28-29 0.01027272751634927 30-31 4.0693896441576814E-4 32-33 0.0015930171655787998 34-35 0.0033944664836632367 36-37 0.0012241253401124733 38-39 0.3627166093924888 40-41 0.5156313692771604 42-43 0.04968625502110574 44-45 1.6542234325844234E-6 46-47 0.04349118826607708 48-49 0.010491085009450413 50-51 1.985068119101308E-4 52-53 0.0012456302447360708 54-55 5.707070842416261E-4 56-57 0.015371044135574462 58-59 0.03464770979548075 60-61 1.1579564028090964E-4 62-63 8.932806535955885E-5 64-65 0.0012572098087641618 66-67 1.9188991817979312E-4 68-69 0.0012439760213034865 70-71 4.549114439607164E-4 72-73 0.0029742937317867933 74-75 3.67237602033742E-4 76-77 8.271117162922117E-5 78-79 0.026528781188356398 80-81 4.5821989082588533E-4 82-83 0.0019238618520956846 84-85 3.24227792786547E-4 86-87 1.2902942774158503E-4 88-89 0.02469590162505286 90-91 0.03250549045028392 92-93 0.015334651220057604 94-95 0.027317845765699168 96-97 0.015288332963945241 98-99 4.1521008157869026E-4 100-101 0.026444415793294592 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.0225663E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.570856215825852 #Duplication Level Percentage of deduplicated Percentage of total 1 69.94300542671719 9.491864699487063 2 10.696342298559443 2.9031704673801277 3 4.706253572553478 1.9160367164501992 4 2.8681503283036416 1.5569302284312971 5 1.9186250128271483 1.3018692090582127 6 1.298684311116808 1.0574554833549028 7 0.9888523520113702 0.9393701161479261 8 0.7877395419132208 0.8552240047059873 9 0.6661532289020827 0.813624271842446 >10 4.854198702905601 13.365572468975346 >50 0.6326135243457914 6.016142551119878 >100 0.5101462664644612 13.896969367073222 >500 0.062499769391124775 5.900698544794765 >1k 0.0543819879180685 14.94412700058155 >5k 0.00717151771896119 6.6639326592020325 >10k+ 0.005182158351715107 18.377012211395268 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 392152 1.2974140550696935 No Hit CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC 292504 0.9677339418493482 No Hit CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT 93825 0.31041502712446706 No Hit CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 87293 0.2888042522011841 No Hit CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT 82076 0.2715440849055983 No Hit CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC 81962 0.27116692196296904 No Hit GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC 77601 0.2567387851839677 No Hit CCGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCT 76280 0.25236832687507965 No Hit CTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA 71701 0.2372189486794715 No Hit CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC 68624 0.22703885767534696 No Hit CCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCC 63155 0.2089449617697385 No Hit CGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTC 58948 0.1950263258079732 No Hit CCCGTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGA 53200 0.17600937322698265 No Hit CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC 51255 0.16957444407422922 No Hit GTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCAT 51008 0.16875725769853253 No Hit CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT 49361 0.16330824571159944 No Hit CTCACTTCTATGAAATAAAACTCCAGCAATACTAACTATAATCTCACTGA 46246 0.1530024337265985 No Hit GCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGT 46112 0.15255910184666585 No Hit CCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGC 44696 0.14787434108558678 No Hit CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT 44399 0.14689173236663164 No Hit CTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCA 44385 0.14684541411051924 No Hit CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG 44199 0.14623004299359788 No Hit CATTATTCTTATAGGAATTAACATTATTATTACAGGTATATACTCAATAT 43413 0.14362960375757514 No Hit GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC 43136 0.14271316397592337 No Hit GCCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCG 41742 0.138101189045878 No Hit CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT 41032 0.13575219177160813 No Hit CTCAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTAC 40723 0.13472988169027095 No Hit CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC 40542 0.13413105280767537 No Hit CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA 38801 0.12837104681541642 No Hit ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG 38762 0.12824201738767482 No Hit GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCC 38615 0.12775567569849502 No Hit ATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTG 38195 0.12636612801512412 No Hit CCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAATACACGACAGC 37899 0.12538682774303414 No Hit CTGAAATTTGTGGATCTAACCATAGCTTTATGCCCATTGTCCTAGAAATG 37529 0.12416270240292165 No Hit CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA 37407 0.12375907188537105 No Hit CCCAAATAAATCTGTACTCAGAAAAAGCAAATCCATATGAATGCGGATTC 37306 0.123424918751989 No Hit CTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGA 34778 0.11506116507684215 No Hit GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA 34070 0.11271878469630262 No Hit CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA 33603 0.11117374001026875 No Hit CTCCCTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAA 33151 0.10967832202721244 No Hit CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG 32446 0.1073458669872684 No Hit CCCCCTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATT 31907 0.1055626141269424 No Hit GGCTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAA 31507 0.10423923538087486 No Hit CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT 31382 0.10382567952272874 No Hit CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT 30293 0.10022278088655986 No Hit CTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCAATTACAT 30231 0.10001765718091941 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.903967300899239E-4 0.0 0.0 0.0 0.0 2 3.903967300899239E-4 0.0 0.0 0.0 0.0 3 3.903967300899239E-4 0.0 0.0 6.6168937303376934E-6 0.0 4 3.937051769550928E-4 0.0 0.0 9.92534059550654E-6 0.0 5 3.937051769550928E-4 0.0 0.0 9.92534059550654E-6 0.0 6 3.937051769550928E-4 0.0 0.0 1.3233787460675387E-5 0.0 7 3.937051769550928E-4 0.0 0.0 2.3159128056181927E-5 0.0 8 3.937051769550928E-4 0.0 0.0 2.3159128056181927E-5 0.0 9 3.970136238202616E-4 0.0 0.0 2.3159128056181927E-5 0.0 10-11 4.003220706854305E-4 0.0 0.0 2.8121798353935197E-5 0.0 12-13 4.019762941180149E-4 0.0 0.0 2.977602178651962E-5 0.0 14-15 4.0693896441576814E-4 0.0 0.0 3.3084468651688465E-5 0.0 16-17 4.1521008157869026E-4 0.0 0.0 4.135558581461058E-5 0.0 18-19 4.3671498620228774E-4 0.0 0.0 5.128092641011713E-5 0.0 20-21 4.631825611236386E-4 0.0 0.0 6.286049043820809E-5 0.0 22-23 5.111550406685869E-4 0.0 0.0 6.286049043820809E-5 0.0 24-25 6.203337872191588E-4 0.0 0.0 6.782316073596136E-5 0.0 26-27 7.311667572023151E-4 0.0 0.0 8.271117162922116E-5 0.0 28-29 9.114771113540173E-4 0.0 0.0 8.601961849439001E-5 0.0 30-31 0.0012522471384664086 0.0 0.0 8.767384192697444E-5 0.0 32-33 0.0019188991817979313 0.0 0.0 9.429073565731213E-5 0.0 34-35 0.0030752013611744433 0.0 0.0 1.0090762938764983E-4 0.0 36-37 0.004428356129028501 0.0 0.0 1.058702996854031E-4 0.0 38-39 0.006673137327045563 0.0 0.0 1.058702996854031E-4 0.0 40-41 0.00995180817042789 0.0 0.0 1.0752452311798752E-4 0.0 42-43 0.013963299994445118 0.0 0.0 1.1579564028090964E-4 0.0 44-45 0.019160870019625374 0.0 0.0 1.3068365117416945E-4 0.0 46-47 0.02490267955412591 0.0 0.0 1.3895476833709157E-4 0.0 48-49 0.03122677573689616 0.0 0.0 1.4391743863484483E-4 0.0 50-51 0.03865258472576764 0.0 0.0 1.4557166206742925E-4 0.0 52-53 0.04758042859142577 0.0 0.0 1.4557166206742925E-4 0.0 54-55 0.05854958417289308 0.0 0.0 1.4557166206742925E-4 0.0 56-57 0.0713665073285572 0.0 0.0 1.4722588550001366E-4 0.0 58-59 0.08585915882142933 0.0 0.0 1.5715122609552022E-4 0.0 60-61 0.10224424192117804 0.0 0.0 1.6045967296068908E-4 0.0 62-63 0.12103291166847192 0.0 0.0 1.6707656669102678E-4 0.0 64-65 0.14402496315796282 0.0 0.0 1.703850135561956E-4 0.0 66-67 0.17070758712554956 0.0 0.0 1.7203923698878002E-4 0.0 68-69 0.20255635087309748 0.0 0.0 1.7534768385394888E-4 0.0 70-71 0.23934628001377506 0.0 0.0 1.7700190728653332E-4 0.0 72-73 0.281532285991543 0.0 0.0 1.7865613071911773E-4 0.0 74-75 0.33469902711480637 0.0 0.0 1.8692724788203985E-4 3.3084468651688467E-6 76-77 0.3974503388064639 0.0 0.0 2.4978773832024794E-4 3.3084468651688467E-6 78-79 0.4694504137097009 0.0 0.0 2.679841960786766E-4 3.3084468651688467E-6 80-81 0.5507985052304726 0.0 0.0 2.81217983539352E-4 3.3084468651688467E-6 82-83 0.6415640907529473 0.0 0.0 2.894891007022741E-4 3.3084468651688467E-6 84-85 0.7623769907048854 0.0 0.0 2.911433241348585E-4 3.3084468651688467E-6 86-87 0.9031662928287132 0.0 0.0 2.944517710000274E-4 3.3084468651688467E-6 88-89 1.0575417320043567 0.0 0.0 2.961059944326118E-4 3.3084468651688467E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCGAAA 62695 0.0 63.03296 1 CCAAACG 71235 0.0 55.5989 8 CCGAAAC 71130 0.0 55.59384 2 CGAAACC 79165 0.0 49.86078 3 CAAACGA 84315 0.0 47.02491 9 GTCCGTA 1270 0.0 44.17999 1 AAACCAA 92090 0.0 43.276146 5 CGCCCAT 1335 0.0 43.097435 1 GAAACCA 93405 0.0 42.560055 4 GATATAT 23250 0.0 41.22925 5 ACTCGTC 58085 0.0 41.070423 42-43 TCCGCTC 2610 0.0 40.75176 2 CCAGATA 21145 0.0 40.73506 2 TCGTCTA 59410 0.0 40.12135 44-45 CTAACGA 57855 0.0 39.76184 94-95 CGCTCGT 1865 0.0 39.26352 1 ACCAAAC 103050 0.0 38.861286 7 CAGATAT 22950 0.0 38.192974 3 CTCCGCT 3540 0.0 38.147175 1 CGCCTAT 1565 0.0 37.97895 1 >>END_MODULE