FastQCFastQC Report
Fri 27 May 2016
SRR2032141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2032141_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32704466
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC2730070.8347697834295781No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT2302260.7039589027382377No Hit
CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT834480.2551578123917388No Hit
CCGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCT748410.22884030578576028No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT733430.2242598916001258No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC712110.21774090425448317No Hit
CTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA698520.21358550847459182No Hit
CCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCC608900.1861825232064636No Hit
CGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTC590410.18052886110416846No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC566890.17333718275663024No Hit
CTGAAATTTGTGGATCTAACCATAGCTTTATGCCCATTGTCCTAGAAATG544310.16643292692808376No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA523560.16008822770565953No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC494780.15128820632631643No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC491700.1503464389236626No Hit
GTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCAT486240.14867694216441266No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA437900.13389608624094337No Hit
CCCGTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGA419390.1282363087659037No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG417860.12776848275094907No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA408080.12477806547888597No Hit
CCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGC395400.12090091915886961No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG388370.118751365639176No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA379610.1160728323770827No Hit
GCCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCG377410.1154001413751871No Hit
CTCCCTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAA369450.112966223022874No Hit
GCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGT366430.11204280173845371No Hit
GGCTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAA355730.10877107732014336No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC348080.1064319472453701No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT342390.10469212369955834No Hit
CTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCA342140.10461568154025201No Hit
CCCATTTATTATCATTGGCCAACTAGCCTCCATCTCATACTTCTCAATCA342000.10457287393104049No Hit
CCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTC341770.10450254714447868No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA340490.10411116328883033No Hit
CTCAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTAC337990.10334674169576717No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG327230.10005667115922333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA384250.061.4362871
CCAAACG428150.054.8383038
CCGAAAC432450.054.3507922
CGAAACC522800.044.803113
CAAACGA549150.042.9027189
ACTCGTC352050.039.8827642-43
TCCGCTC31100.038.780192
TCGTCTA366550.038.2485944-45
CTAACGA352800.038.05154894-95
GTCCGTA15250.037.7255131
CGCTCGT19250.036.555321
GATATAT187000.035.0128975
CAACTCG403700.034.97218340-41
TACGCAT113550.034.650659
AAACCAA695150.034.478335
TAGGTAG395100.034.42429776-77
GAAACCA694250.034.3413664
CGAGCTA418100.034.3042312-13
CTCCGCT41800.033.1006361
CTACGCA120550.032.756258
Produced by FastQC (version 0.11.5)