FastQCFastQC Report
Fri 27 May 2016
SRR2032140_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2032140_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30508921
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA2233230.7319924555837292No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT2132520.698982438611972No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA1849940.606360349485975No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC1635820.5361775986767936No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA1534660.5030200838633395No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT1474340.4832488176163293No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC1184580.3882733184828136No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA1182380.3875522179234067No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG1102020.3612123811261631No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG953880.3126560916395568No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT943620.30929314084886844No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGC904230.29638216310566995No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT869490.2849953297266724No Hit
CGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGA833330.27314305871387584No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA786440.2577737836090631No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG784400.25710512672670394No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA777360.254797604936602No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGG764980.2507397754250306No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA657740.2155894008837612No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT654990.2146880251845026No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA653280.21412753338605453No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA650950.21336382233904636No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG614190.20131488753732063No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT591060.19373349847410204No Hit
AACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCC588760.1929796206165403No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT588180.19278951228724214No Hit
CCAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTC569920.18680437764416513No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC549540.18012436428020512No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC526490.17256919705551044No Hit
CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT526220.17248069835049232No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC524860.17203492709558624No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA506650.16606618110158666No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA499170.16361443919960328No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA492130.16130691740950132No Hit
CAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCA490610.16080870247754747No Hit
CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT488400.1600843241883251No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG484280.15873389950434497No Hit
CAAAGACCAAGAGGTACAGGTGCAAGGGAGAGAAGAAGGGCATGGCCAGA469220.1537976384022234No Hit
GTTTATTTTACACTGGGCGAATTGCTTCTGTACAATAGAAAGCAGTCTGT441570.14473471546240524No Hit
GTGAAATAAATGCTTTTTATTTGTCAGAAGACAGATTTTCAAATGTCTTT434390.14238130545488648No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG429780.14087027200994753No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT429030.1406244422737861No Hit
CGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAG425920.13960506830117No Hit
CTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGA417630.1368878302841323No Hit
CGAACGTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGA412040.13505557931727574No Hit
CGCAGGATATTTTATTGAGACAAGGTGGGTCAACGGGAAGCTGAGCAGTG398020.1304602021159647No Hit
CTCAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCT392410.12862139568947717No Hit
CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT389530.12767740950261727No Hit
AATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAA385340.12630404070992873No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA380040.12456684390772128No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG376630.12344913804064063No Hit
CTCAGGCTTTATTCAAAGACCAAGAGGTACAGGTGCAAGGGAGAGAAGAA366640.12017468595497036No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT362380.11877837305357342No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT358130.11738533788199196No Hit
GAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGT345980.11340289615617676No Hit
TTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGAT344310.11285551527699061No Hit
GTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCT344280.11284568208754417No Hit
AGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATAATAAAT343260.11251135364636461No Hit
GTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGAGGTTATTCCTC338560.1109708206330863No Hit
TATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAAT337400.11059060397448994No Hit
ATTAAGTTGAGAGCGCTTATTTGTAAGGTGGCTCTATTTCTCTTGTCCTT334950.10978756016969593No Hit
CAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTG333660.10936473302349826No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT329880.10812575115324466No Hit
CTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAATTAGCAAGAG323750.10611650277635187No Hit
GCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAG318490.10439241689340636No Hit
CTTTATTCAAAGACCAAGAGGTACAGGTGCAAGGGAGAGAAGAAGGGCAT315080.10327471102632571No Hit
ATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTA311030.10194723045105396No Hit
GATTTATTTACCTGAAATCATGTCTGTGCAATACCTGTTACCAGCACAGA307070.10064924944412161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT412150.089.625091
GCGTGTT459650.079.425922
GTAAACG252350.064.080171
TAAACGC274900.058.8958782
CGTGTTT663350.054.9717333
CGAACGT80600.052.3146551
TCGAACG32650.049.3116042
GTCGAAC36550.045.363551
GTGTTTT860250.041.750234
AAACGCT403650.040.5338943
GAACGTT111850.038.045622
GTTTTTT2760950.037.0931781
GTAGATA342300.035.991152
ACGCTTC453800.035.8884665
ACGTTTA123250.035.7608574
TAGATAT345050.035.7044223
ATCAATA543550.035.170074
CGCGTTT9100.035.079111
AACGCTT467400.034.9962354
ATTGAAG649500.034.0507166
Produced by FastQC (version 0.11.5)