FastQCFastQC Report
Fri 27 May 2016
SRR2032140_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2032140_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30508921
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC3048830.9993240993347486No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT2680410.8785659774726219No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC1891830.6200907596830448No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA1462550.47938437416387164No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT1337770.4384848615262401No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC1103610.3617335401668253No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA1065140.3491241135666515No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCC857890.2811931631407089No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT842110.27602090549187236No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG800050.2622347738879392No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC790850.2592192624576923No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT790100.25897343272153084No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC719980.23598999125534464No Hit
CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT680870.22317078994697975No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA675250.22132870579067676No Hit
CTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA651670.21359981888576132No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA597070.19570341409320902No Hit
CTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGA587780.19265840309462273No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA550280.18036691628655108No Hit
CTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCG520020.17044850586489113No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG517000.1694586314606144No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG503430.16501075210100025No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC464300.15218499533300442No Hit
GCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGT464040.15209977435780178No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC458210.15018885787537356No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT434950.14256485832455365No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG427250.1400410063666296No Hit
CCCCTTTCCTGCTCTTGCCTGTGAACAATGGTTAATTGTTCCCAAGAGAG424200.13904129877290644No Hit
CTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCA422050.13833658686257702No Hit
CTGGTATCTTTCAGAGTGCCAAGTGATTTCAGAATGCAAAGAATTCTTTG421550.13817270037180274No Hit
CCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGC419690.13756304262612237No Hit
CTCAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTAC412030.1350523015874603No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT401800.13169918398621833No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA388980.12749713436276555No Hit
ATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTG366780.12022057417238716No Hit
CTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAATAGA362890.1189455372741632No Hit
CCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTC359200.11773605497224894No Hit
CTGAAATTTGTGGATCTAACCATAGCTTTATGCCCATTGTCCTAGAAATG354070.11605457957690472No Hit
CAATAAACTAGGAGGTGTCCTAGCCTTAATCTTATCTATCCTAATTTTAG352440.11552030961698055No Hit
CTAATTTTAGCCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCT347280.1138290010321899No Hit
ATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACT346480.11356678264695104No Hit
GCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCC330670.10838469180866803No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC325030.10653605219273406No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT324580.1063885543510372No Hit
GTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCAT323110.10590672806816079No Hit
CGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCAC322090.10557239962698123No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC313000.10259294322470466No Hit
CCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCT307830.10089835691009853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA433950.063.264911
CCGAAAC597300.046.074012
CCCGATC13050.045.9077381
CCAAACG622100.044.279828
CGAAACC688600.039.7925763
ACTCGTC539250.039.0121542-43
TACGCAT97600.038.027749
TCCGCTC26100.038.021212
CAAACGA729100.037.8860639
TCGTCTA555150.037.88099744-45
CTAACGA550450.037.15506494-95
CTACGCA101250.036.515828
CTCCGCT32200.036.472621
CTCGTAC67700.036.3101231
CTCTACG103750.035.9181636
TAGGTAG576250.035.69767876-77
CGGCAAT142600.035.443741
CGAGCTA600700.035.41591312-13
TCGTACC68000.035.1918222
TCCACTC882850.034.952622
Produced by FastQC (version 0.11.5)