##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2032139_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34749897
Sequences flagged as poor quality	0
Sequence length	101
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12649131017568	34.0	31.0	34.0	30.0	34.0
2	32.431281307107184	34.0	31.0	34.0	30.0	34.0
3	32.516903258734835	34.0	31.0	34.0	31.0	34.0
4	35.845185440405764	37.0	35.0	37.0	35.0	37.0
5	35.93714191440625	37.0	35.0	37.0	35.0	37.0
6	35.88129426685783	37.0	35.0	37.0	35.0	37.0
7	35.95257318316656	37.0	35.0	37.0	35.0	37.0
8	35.92224092059899	37.0	35.0	37.0	35.0	37.0
9	37.72424847762858	39.0	38.0	39.0	35.0	39.0
10-11	37.71938559990552	39.0	38.0	39.0	35.0	39.0
12-13	37.59066176512696	39.0	38.0	39.0	35.0	39.0
14-15	39.01320708950591	41.0	39.0	41.0	36.0	41.0
16-17	38.82002038739856	41.0	39.0	41.0	35.0	41.0
18-19	38.68443532365002	40.5	38.5	41.0	34.5	41.0
20-21	38.55355302204205	41.0	39.0	41.0	34.0	41.0
22-23	38.422242057293005	41.0	39.0	41.0	34.0	41.0
24-25	38.33805688690242	41.0	39.0	41.0	34.0	41.0
26-27	38.17215271170444	41.0	39.0	41.0	34.0	41.0
28-29	38.04892286443324	41.0	39.0	41.0	34.0	41.0
30-31	37.906728371022226	40.0	38.0	41.0	33.0	41.0
32-33	37.79276199005712	40.0	38.0	41.0	33.0	41.0
34-35	37.756700660148724	40.0	38.0	41.0	33.0	41.0
36-37	37.64748829327465	40.0	38.0	41.0	33.0	41.0
38-39	37.53656085656887	40.0	38.0	41.0	33.0	41.0
40-41	37.50489427349957	40.0	38.0	41.0	32.5	41.0
42-43	37.4158465275451	40.0	38.0	41.0	32.0	41.0
44-45	37.303888051812066	40.0	38.0	41.0	32.0	41.0
46-47	37.209557254227256	40.0	38.0	41.0	31.5	41.0
48-49	37.08727969754845	40.0	38.0	41.0	31.0	41.0
50-51	36.98302563314073	40.0	37.5	41.0	31.0	41.0
52-53	36.90089225300437	40.0	37.0	41.0	31.0	41.0
54-55	36.7343054858551	40.0	37.0	41.0	30.0	41.0
56-57	36.63781430776615	40.0	37.0	41.0	30.0	41.0
58-59	36.48888048502705	40.0	36.0	41.0	30.0	41.0
60-61	36.384784867132126	40.0	36.0	41.0	30.0	41.0
62-63	36.294702916673394	39.0	36.0	41.0	30.0	41.0
64-65	36.037392902200544	39.0	35.0	41.0	29.5	41.0
66-67	35.769389719342186	39.0	35.0	41.0	29.0	41.0
68-69	35.49038261897582	38.5	35.0	40.5	29.0	41.0
70-71	35.13793022177879	37.5	35.0	40.0	28.0	41.0
72-73	34.7731659607509	37.0	35.0	40.0	28.0	41.0
74-75	34.35133344424014	36.5	34.0	39.0	27.5	41.0
76-77	34.14365461284677	36.0	34.0	39.0	28.0	41.0
78-79	34.00991152578092	36.0	34.0	38.5	29.0	39.5
80-81	33.68889514981872	35.0	34.0	37.0	29.0	39.0
82-83	33.36379772578894	35.0	34.0	37.0	29.0	39.0
84-85	33.07501131586088	35.0	34.0	36.5	29.0	38.0
86-87	32.795567782546236	35.0	34.0	36.0	28.0	37.0
88-89	32.56760612844406	35.0	34.0	36.0	27.5	37.0
90-91	32.365121499496816	35.0	34.0	35.5	27.5	36.0
92-93	32.17227587753713	35.0	34.0	35.0	27.0	36.0
94-95	31.986120246629795	35.0	34.0	35.0	27.0	36.0
96-97	31.87504283825647	35.0	34.0	35.0	26.5	36.0
98-99	31.719678924515947	35.0	34.0	35.0	26.0	36.0
100-101	31.38652819604041	35.0	33.5	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.07002653588302366
1101	2	0.11881846014603781
1101	3	0.10178522674424784
1101	4	0.17711928345720906
1101	5	0.097185112436172
1101	6	0.10130908366807745
1101	7	0.03787830361709155
1101	8	0.07282754809210701
1101	9	0.038038956401329926
1101	10-11	0.0754994708809349
1101	12-13	0.054790634440458064
1101	14-15	0.0680806827904874
1101	16-17	0.09491410901692632
1101	18-19	0.024050887351691586
1101	20-21	0.01740247306793208
1101	22-23	0.0043978861708708905
1101	24-25	0.001804071245231853
1101	26-27	-0.0018568264237899257
1101	28-29	-0.06165117724277991
1101	30-31	-0.03823260872076162
1101	32-33	-0.01876039781458161
1101	34-35	-0.04407240521460665
1101	36-37	-0.06185615071034789
1101	38-39	-0.05242729327516571
1101	40-41	-0.03002854060581228
1101	42-43	-0.06258220258040126
1101	44-45	-0.06707890220484103
1101	46-47	-0.10063851703486648
1101	48-49	-0.05805205072942954
1101	50-51	-0.07119630818581157
1101	52-53	-0.18416381284303185
1101	54-55	-0.2088994996897
1101	56-57	-0.21349773048186904
1101	58-59	-0.14733891344704375
1101	60-61	-0.1297585815106217
1101	62-63	-0.17251306351848683
1101	64-65	-0.18237183084276154
1101	66-67	-0.21295363608869877
1101	68-69	-0.17207466555631612
1101	70-71	-0.19287898329812236
1101	72-73	-0.15363854929123733
1101	74-75	-0.24149416168532412
1101	76-77	-0.16591775341829873
1101	78-79	-0.14800102013366967
1101	80-81	-0.14869967319408062
1101	82-83	-0.10804775769216945
1101	84-85	-0.11336234589194305
1101	86-87	-0.12709930148164972
1101	88-89	-0.1289061395386426
1101	90-91	-0.15543083185562523
1101	92-93	-0.12512933117594827
1101	94-95	-0.13474902453918602
1101	96-97	-0.1257181789189481
1101	98-99	-0.15588742428781543
1101	100-101	-0.17213827528665604
1102	1	-8.643560038734677E-4
1102	2	0.033755602579404353
1102	3	0.020656061452875463
1102	4	0.14122731536870958
1102	5	0.04380719409845568
1102	6	0.062291321574200254
1102	7	0.03527736844870333
1102	8	0.01594509257952126
1102	9	-0.013827044670591704
1102	10-11	0.012129487983138176
1102	12-13	0.020791010659685583
1102	14-15	0.07253421584834285
1102	16-17	0.06714159520242191
1102	18-19	0.010456307497392459
1102	20-21	0.03834995037397704
1102	22-23	0.030365067797333722
1102	24-25	-0.0011405332279252889
1102	26-27	-0.013939393456624316
1102	28-29	-0.03431035332035748
1102	30-31	-0.05153568002782549
1102	32-33	-0.07124474650348134
1102	34-35	-0.10161465679836112
1102	36-37	-0.08604256762864537
1102	38-39	-0.07716909057591437
1102	40-41	-0.06112617063247683
1102	42-43	-0.0614874235004379
1102	44-45	-0.05340276953919698
1102	46-47	-0.05324733404513893
1102	48-49	-0.08153633429674301
1102	50-51	0.006164769312562157
1102	52-53	-0.008139323522946995
1102	54-55	-0.054354641713842966
1102	56-57	-0.04437899436951653
1102	58-59	-0.06822743425041722
1102	60-61	-0.06651625466327715
1102	62-63	-0.10582326083791571
1102	64-65	-0.11633746361771813
1102	66-67	-0.13800886086625752
1102	68-69	-0.16043821218779897
1102	70-71	-0.13303090264555095
1102	72-73	-0.11642228807707511
1102	74-75	-0.13678296945041524
1102	76-77	-0.08270456823733241
1102	78-79	-0.060879177866766554
1102	80-81	-0.08719078572990924
1102	82-83	-0.06511995216548883
1102	84-85	-0.07480796085678065
1102	86-87	-0.11687901445678506
1102	88-89	-0.07036794817985026
1102	90-91	-0.09254191763456987
1102	92-93	-0.11194187590557902
1102	94-95	-0.10808262795810464
1102	96-97	-0.10558787411285309
1102	98-99	-0.09963139676875699
1102	100-101	-0.08248844867856775
1103	1	-0.05726472631631907
1103	2	0.011600766025104292
1103	3	-0.023764022808201446
1103	4	0.12447826835819598
1103	5	0.012901023850631077
1103	6	-0.004222611264616205
1103	7	-0.007408528138739712
1103	8	-0.031232645994066388
1103	9	-0.024038154815308133
1103	10-11	-0.039141797653591937
1103	12-13	-0.03821122490767692
1103	14-15	-0.030138748855939923
1103	16-17	-0.01099638265650782
1103	18-19	-0.04319220970805304
1103	20-21	-0.05249931753657222
1103	22-23	-0.059710391776576444
1103	24-25	-0.10976469364451447
1103	26-27	-0.09050647773366904
1103	28-29	-0.09891727407974571
1103	30-31	-0.09253925365817395
1103	32-33	-0.09852012586729586
1103	34-35	-0.13574354313259107
1103	36-37	-0.1363766767915422
1103	38-39	-0.09959132107812962
1103	40-41	-0.12991882796515597
1103	42-43	-0.1067226812497637
1103	44-45	-0.15218402936692144
1103	46-47	-0.24629277162225094
1103	48-49	-0.13613832757113897
1103	50-51	-0.12664211754607635
1103	52-53	-0.18789174559196908
1103	54-55	-0.1600034119149214
1103	56-57	-0.23647756668584208
1103	58-59	-0.24223077879906896
1103	60-61	-0.2420398942934554
1103	62-63	-0.20428672469243025
1103	64-65	-0.249089580553715
1103	66-67	-0.19302227214859613
1103	68-69	-0.15220974664149622
1103	70-71	-0.15325555501158306
1103	72-73	-0.19780556979911523
1103	74-75	-0.2001586775338282
1103	76-77	-0.17700857107156764
1103	78-79	-0.16359391908620324
1103	80-81	-0.16226139209149437
1103	82-83	-0.16003330528163673
1103	84-85	-0.15107043414598564
1103	86-87	-0.15654820719549178
1103	88-89	-0.15512647489180154
1103	90-91	-0.15845420796909337
1103	92-93	-0.17567403638276602
1103	94-95	-0.17993725793534665
1103	96-97	-0.20114971640218116
1103	98-99	-0.2284915069657032
1103	100-101	-0.2858303478730022
1104	1	-0.0809830349529932
1104	2	-0.02894094452545204
1104	3	-0.06255007622736031
1104	4	0.0657891309153058
1104	5	-0.01723465256080914
1104	6	0.00823266440935555
1104	7	-0.02433247335412858
1104	8	-0.015318542297990234
1104	9	-0.08328446914640608
1104	10-11	-0.06816177503243637
1104	12-13	-0.06517994468608634
1104	14-15	-0.04782284344821619
1104	16-17	-0.025819934631819308
1104	18-19	-0.03520879508903363
1104	20-21	-0.04194542506058241
1104	22-23	-0.10839857761584426
1104	24-25	-0.0511574550604621
1104	26-27	-0.08372640883179372
1104	28-29	-0.11066300743228652
1104	30-31	-0.1734469399271319
1104	32-33	-0.200552220679306
1104	34-35	-0.1830774243387765
1104	36-37	-0.17347763613998524
1104	38-39	-0.16995977331991696
1104	40-41	-0.1284885093707402
1104	42-43	-0.1476300449482295
1104	44-45	-0.11153114268817887
1104	46-47	-0.15589448186275945
1104	48-49	-0.20974486866082032
1104	50-51	-0.10908489399258059
1104	52-53	-0.17614017290489414
1104	54-55	-0.1698881907792753
1104	56-57	-0.1369442349608434
1104	58-59	-0.1335406809539279
1104	60-61	-0.12659029463144833
1104	62-63	-0.13949457216779138
1104	64-65	-0.12987659506585203
1104	66-67	-0.1573999122499643
1104	68-69	-0.1546864082435775
1104	70-71	-0.15838329459346312
1104	72-73	-0.14114438469049162
1104	74-75	-0.15447281784139477
1104	76-77	-0.14757679272544522
1104	78-79	-0.1481141403526962
1104	80-81	-0.12886699235989596
1104	82-83	-0.08427153017787958
1104	84-85	-0.0926833167644574
1104	86-87	-0.12154864672687893
1104	88-89	-0.1483884939191995
1104	90-91	-0.13444340844015557
1104	92-93	-0.1061223443557111
1104	94-95	-0.13070972679189197
1104	96-97	-0.15891297024782247
1104	98-99	-0.14179333100331348
1104	100-101	-0.17550972277901877
1105	1	-0.07669029134923733
1105	2	-0.07167493029236027
1105	3	-0.04243472942038551
1105	4	0.10471730627473619
1105	5	0.03163393868952369
1105	6	0.002395409346739541
1105	7	-0.02742930565153756
1105	8	-0.034652578068786966
1105	9	-0.048338822109386115
1105	10-11	-0.046084129345445035
1105	12-13	-0.03917711004985591
1105	14-15	-0.07975008393282934
1105	16-17	-0.05889726729581213
1105	18-19	-0.08886087026338885
1105	20-21	-0.09111067461229538
1105	22-23	-0.07653213093346523
1105	24-25	-0.0804789895324518
1105	26-27	-0.07145524019317406
1105	28-29	-0.11105477150025678
1105	30-31	-0.09781345805406261
1105	32-33	-0.10343049045337693
1105	34-35	-0.12866860042352357
1105	36-37	-0.11206348987735026
1105	38-39	-0.12091138092438314
1105	40-41	-0.1433858964001118
1105	42-43	-0.09994233967699984
1105	44-45	-0.1248628836646617
1105	46-47	-0.09812684826894724
1105	48-49	-0.09615897792507155
1105	50-51	-0.0966150867110045
1105	52-53	-0.09475922981747686
1105	54-55	-0.10045535487935098
1105	56-57	-0.1071498087374394
1105	58-59	-0.09197929909672098
1105	60-61	-0.10695229452216637
1105	62-63	-0.12597326991021873
1105	64-65	-0.13488385373492662
1105	66-67	-0.12499848848135997
1105	68-69	-0.12711847799778297
1105	70-71	-0.18422800545863538
1105	72-73	-0.16811587323589094
1105	74-75	-0.15147641014446123
1105	76-77	-0.11354291254119175
1105	78-79	-0.1002930393928736
1105	80-81	-0.11754627225470671
1105	82-83	-0.07580421838692786
1105	84-85	-0.08251520685986691
1105	86-87	-0.15237755763870808
1105	88-89	-0.09724611940387717
1105	90-91	-0.13786712482602326
1105	92-93	-0.17400390030636004
1105	94-95	-0.19848117164783474
1105	96-97	-0.18887553289055958
1105	98-99	-0.1904107272068778
1105	100-101	-0.2341341188909638
1106	1	-0.11416031445127572
1106	2	-0.08180771082979987
1106	3	-0.11329210679441815
1106	4	0.06051403834901237
1106	5	-0.010364929515226606
1106	6	-0.0138508577561538
1106	7	-0.03365929801618961
1106	8	-0.0572125861338435
1106	9	-0.10428658616147857
1106	10-11	-0.06760072551936958
1106	12-13	-0.0626654936767963
1106	14-15	-0.062055328700424184
1106	16-17	-0.04679269215152004
1106	18-19	-0.07388543950920479
1106	20-21	-0.11136263167157523
1106	22-23	-0.11490872949440245
1106	24-25	-0.08472770186268974
1106	26-27	-0.12547528786694784
1106	28-29	-0.0772381515078493
1106	30-31	-0.09703182826223866
1106	32-33	-0.1264643881761529
1106	34-35	-0.1055410746647425
1106	36-37	-0.1486466690661672
1106	38-39	-0.11680399946560271
1106	40-41	-0.1280267434050657
1106	42-43	-0.13421837511235424
1106	44-45	-0.17496457129664833
1106	46-47	-0.1676260505749596
1106	48-49	-0.16116538254131285
1106	50-51	-0.14918787818138668
1106	52-53	-0.16906763947150694
1106	54-55	-0.15057951195596075
1106	56-57	-0.1548695972992462
1106	58-59	-0.18005206753159086
1106	60-61	-0.15103947670687745
1106	62-63	-0.17275344717896957
1106	64-65	-0.1892335979294515
1106	66-67	-0.18954640214952434
1106	68-69	-0.1971184025083801
1106	70-71	-0.1773598465650963
1106	72-73	-0.15692921457139164
1106	74-75	-0.147707993084218
1106	76-77	-0.15230818617758501
1106	78-79	-0.12756567975907984
1106	80-81	-0.1287555713220101
1106	82-83	-0.14032871280203096
1106	84-85	-0.13227958024332054
1106	86-87	-0.1596435006337913
1106	88-89	-0.14074805817292457
1106	90-91	-0.1567404417289211
1106	92-93	-0.12555535003648544
1106	94-95	-0.15076704249885609
1106	96-97	-0.17419495510527483
1106	98-99	-0.20632120859612968
1106	100-101	-0.21083655284250824
1107	1	-0.1557040755544037
1107	2	-0.08711224181689659
1107	3	-0.1319823568347971
1107	4	0.055299942222617915
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2216	18-19	-0.13004424373333734
2216	20-21	-0.14382127681220425
2216	22-23	-0.14646822089958533
2216	24-25	-0.14856654019333604
2216	26-27	-0.19830939859865282
2216	28-29	-0.1064951930567446
2216	30-31	-0.08311113721006791
2216	32-33	-0.07875478863633134
2216	34-35	-0.07850731639980779
2216	36-37	-0.07333753285035272
2216	38-39	-0.09577599018606975
2216	40-41	-0.14222932807440714
2216	42-43	-0.1800496278982564
2216	44-45	-0.14101268498171748
2216	46-47	-0.1278497225085431
2216	48-49	-0.15976572977422876
2216	50-51	-0.11091127634416154
2216	52-53	-0.11106062160294528
2216	54-55	-0.12905394479614074
2216	56-57	-0.14784336591800695
2216	58-59	-0.2514951235193905
2216	60-61	-0.3089537885291449
2216	62-63	-0.2781247867304799
2216	64-65	-0.21661274886120196
2216	66-67	-0.18830195892105905
2216	68-69	-0.2060871846882435
2216	70-71	-0.21394648257086146
2216	72-73	-0.24096567820281223
2216	74-75	-0.20452879404995628
2216	76-77	-0.17795820666010798
2216	78-79	-0.2130357707455559
2216	80-81	-0.15056329585157613
2216	82-83	-0.17518294875771545
2216	84-85	-0.15988233361451876
2216	86-87	-0.1534230784743258
2216	88-89	-0.1543822400932413
2216	90-91	-0.15850949702388561
2216	92-93	-0.14696563870936785
2216	94-95	-0.19653093272555822
2216	96-97	-0.1706823833655271
2216	98-99	-0.19628930686747026
2216	100-101	-0.13656158762529458
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	70258.0
3	20680.0
4	22715.0
5	42227.0
6	135709.0
7	123984.0
8	99764.0
9	124874.0
10	173124.0
11	171961.0
12	132538.0
13	94189.0
14	73188.0
15	64219.0
16	60215.0
17	57149.0
18	56622.0
19	56907.0
20	59770.0
21	66943.0
22	79342.0
23	97852.0
24	122201.0
25	146110.0
26	173352.0
27	208262.0
28	254642.0
29	312846.0
30	389924.0
31	506297.0
32	667618.0
33	879670.0
34	1095896.0
35	1543065.0
36	2776845.0
37	6686280.0
38	1.1812794E7
39	5278162.0
40	11703.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.757198486785246	18.41630743583572	16.507615217766023	38.318878859613015
2	16.857429878678737	21.152846590952752	52.94663403065499	9.043089499713519
3	13.523208777268616	22.637728574823505	45.92656047457502	17.912502173332854
4	18.018075316302497	30.36570140828079	37.873733061135994	13.742490214280725
5	19.82529480120918	33.94993833729323	34.962695738767806	11.26207112272978
6	16.681602038017708	35.58453574632297	34.52585208312888	13.208010132530438
7	14.308623298991066	16.316724561812297	55.3572988185776	14.01735332061905
8	14.937687441203062	21.63870298665921	45.04009896777536	18.383510604362368
9	17.46244903751184	22.838806460405028	42.3817075003044	17.317037001778736
10-11	19.15484227694534	31.007016935195075	34.26621866975098	15.571922118108606
12-13	19.864313306313043	22.8159140776121	39.81797418255808	17.50179843351678
14-15	19.792565509839196	26.470210054181535	38.491485413070336	15.245739022908932
16-17	20.87396810459322	27.171666242181193	36.04397093929283	15.910394713932765
18-19	21.166188027425232	27.839110562866022	33.11365749358765	17.881043916121094
20-21	22.49274565298548	27.376124645915993	33.09020398217301	17.04092571892552
22-23	22.113077349023573	29.340745111270415	31.94494690533123	16.601230634374783
24-25	21.63208339966057	29.185024476584225	30.879256362888746	18.30363576086646
26-27	22.19320706961869	29.30213227865493	30.760517253301188	17.74414339842519
28-29	22.774324303476327	29.086322203952253	30.62486457747366	17.51448891509776
30-31	21.94671760822796	30.98005537485787	29.050404279519416	18.022822737394755
32-33	21.885413802550914	29.34924776477727	30.309485249088524	18.455853183583297
34-35	22.865582249886497	29.749581843948313	28.97685588210017	18.407980024065022
36-37	23.034620253503387	30.086950134532174	28.79286408724636	18.085565524718074
38-39	23.32833893928339	27.876368346072145	30.608031074865032	18.187261639779432
40-41	23.343408742874566	29.80001181560943	28.26308166780853	18.593497773707476
42-43	23.481698596901595	29.356533952827128	28.759613994869387	18.40215345540189
44-45	23.897218086228296	28.75197903441471	28.86194933801429	18.488853541342703
46-47	22.262057691658644	29.392733055666838	28.97505312122217	19.370156131452347
48-49	22.767201978620697	29.027601005604343	29.273218586663763	18.931978429111197
50-51	22.30870026883131	29.166935316596724	29.874103985005874	18.650260429566092
52-53	24.499020462715386	27.863934208995612	28.055265761041497	19.581779567247505
54-55	23.300006907590586	28.030665787089575	29.299343611926005	19.369983693393834
56-57	24.003967542273337	27.976157210264414	29.096513232070986	18.92336201539127
58-59	23.39154905038655	28.51901244225774	28.597035376091917	19.492403131263796
60-61	23.62886246219041	28.269627474245407	28.016460556131285	20.0850495074329
62-63	24.297066365274247	27.903790482639675	28.537985236763223	19.261157915322855
64-65	24.186681976609155	28.022745254346066	28.449441527528062	19.341131241516717
66-67	24.263617861373792	27.832393067228427	27.676920087058072	20.227068984339702
68-69	24.942689402516706	27.219158782377797	28.190711714371634	19.647440100733867
70-71	23.632624362663716	27.12882943746963	28.838231442155642	20.400314757711012
72-73	24.18517450992495	26.610935474718033	28.827885118521746	20.376004896835276
74-75	25.322326402267343	26.840046453573613	28.449649910434836	19.38797723372421
76-77	23.268730952488326	27.26154526627209	28.913035051343922	20.556688729895658
78-79	22.92596751387888	27.62866511669781	28.776376334887477	20.668991034535832
80-81	22.762820511620887	27.092977627107462	29.798722293039276	20.345479568232378
82-83	22.649224038541192	28.067642996139565	28.286316983890387	20.996815981428856
84-85	22.49671752084739	28.01537974758807	28.30718286569637	21.180719865868173
86-87	22.574718044537768	29.209380249697276	28.673340069069003	19.542561636695954
88-89	23.68207412723419	26.396669138138744	30.3366508868251	19.58460584780196
90-91	24.103434915996665	26.869253941036337	28.414596724392393	20.612714418574605
92-93	23.321080650384392	27.01724004708921	29.008128142033435	20.653551160492967
94-95	24.147397645368684	27.36783316746436	27.917898970421817	20.56687021674514
96-97	23.539909339607927	27.132812969825416	28.895214863078543	20.432062827488114
98-99	25.29297205743201	25.999255938495523	28.6714262872241	20.036345716848366
100-101	25.86642132400106	26.548264979942086	27.32049710297054	20.264816593086312
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	43.0
1	131.0
2	280.0
3	469.0
4	749.5
5	1116.0
6	1570.0
7	2090.0
8	2748.5
9	3437.5
10	4342.5
11	5491.0
12	6779.5
13	8404.5
14	10241.0
15	12390.5
16	15145.5
17	18534.5
18	23271.5
19	28832.5
20	40036.5
21	57666.0
22	77876.0
23	107506.0
24	137284.0
25	178644.0
26	227511.0
27	333741.0
28	464370.5
29	649511.0
30	868707.0
31	918770.0
32	1040118.0
33	1299527.0
34	1682029.0
35	1740608.0
36	1489738.0
37	1647436.0
38	1749270.0
39	1764603.0
40	1786099.5
41	1605271.0
42	1325786.0
43	1117198.0
44	1033613.0
45	911820.5
46	788457.0
47	727377.5
48	732045.0
49	730776.0
50	675987.0
51	635614.0
52	644653.0
53	636429.5
54	682537.5
55	602227.5
56	465016.5
57	475006.0
58	452145.0
59	360932.0
60	267669.5
61	193346.5
62	137035.0
63	100935.0
64	81173.5
65	69731.5
66	53077.5
67	33219.5
68	19408.5
69	13647.5
70	10497.5
71	17608.5
72	57536.5
73	128586.0
74	143032.0
75	92835.5
76	54006.0
77	106666.5
78	114105.0
79	33971.0
80	7663.5
81	2009.5
82	703.0
83	350.5
84	227.5
85	171.0
86	125.0
87	92.5
88	79.0
89	65.0
90	46.5
91	35.0
92	30.5
93	25.5
94	22.5
95	20.0
96	18.5
97	17.0
98	15.0
99	16.0
100	47.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014561194238935444
2	0.01687487016148566
3	0.014025940853867856
4	0.005951096775912746
5	0.0057266356789489195
6	0.006926639235793995
7	1.726623822798669E-5
8	0.0
9	0.003033102515382995
10-11	7.280597119467721E-4
12-13	6.071960443508653E-4
14-15	0.004400013041765275
16-17	0.005602894304981681
18-19	0.0064992998396513225
20-21	0.0026028854128689934
22-23	0.008225923662449992
24-25	0.012201475014443929
26-27	0.014459035662753186
28-29	0.019525237729481615
30-31	0.015102203036745693
32-33	0.01739285730832526
34-35	0.016377026959245377
36-37	0.018805811136648837
38-39	0.02670367627276708
40-41	0.022214454333490542
42-43	0.030405845519484564
44-45	0.026343962976350693
46-47	0.028142529458432642
48-49	0.03790946488273045
50-51	0.04613970510473743
52-53	0.039131051237360506
54-55	0.03668643967491472
56-57	0.02878425797923948
58-59	0.03831809918745946
60-61	0.02983030424521834
62-63	0.03585766023997136
64-65	0.04114976225684928
66-67	0.05682462886148986
68-69	0.04832244538739208
70-71	0.045695099470366776
72-73	0.04026774525403629
74-75	0.041932498389851344
76-77	0.03999004658920284
78-79	0.04813251676688423
80-81	0.04839007148711836
82-83	0.05722606890029055
84-85	0.055505200490234545
86-87	0.049610218988562764
88-89	0.04277134979709436
90-91	0.03613104234524782
92-93	0.058982908639988195
94-95	0.09275567061393016
96-97	0.08504485639194845
98-99	0.07758584147745819
100-101	0.2177632353845538
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.4749897E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.989824883977183
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.4771259835181	9.674448243878071
2	8.809772281863777	2.288747984182531
3	4.1391644597085735	1.613010644927892
4	2.293183210855713	1.1915219334356895
5	1.4935141849684688	0.970024386223816
6	1.1344455517593905	0.8841749434656815
7	0.8468758878013547	0.7700538636701615
8	0.6791949066199772	0.7058098319266192
9	0.551483106715271	0.6447302084432764
>10	4.269108414286463	11.34551766845492
>50	0.6216372740945143	5.658693291379052
>100	0.5452544855175497	14.363678288086955
>500	0.06938231747351666	6.2485363934428255
>1k	0.057398924779592325	14.826573680963822
>5k	0.006418277898311184	5.841727271882577
>10k+	0.0060407321395869965	22.97275136563633
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA	254296	0.7317892194040172	No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT	237423	0.6832336798005473	No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA	184779	0.5317397055881922	No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT	179737	0.5172303100639406	No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA	137082	0.3944817447948119	No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG	127208	0.36606727208428846	No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC	125711	0.3617593456464058	No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC	124870	0.35933919458811636	No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA	119677	0.3443952654017938	No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG	105257	0.30289873952719915	No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT	96320	0.277180677686613	No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA	90250	0.25971300001263314	No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT	82871	0.23847840469858084	No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA	81385	0.2342021330307828	No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG	80678	0.23216759462625172	No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA	79413	0.22852729606651787	No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT	75007	0.21584812179443297	No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGG	72728	0.20928982897416934	No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA	72527	0.2087114099935318	No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGC	71567	0.20594881187705388	No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA	71015	0.20436031796007914	No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT	66667	0.19184805065753144	No Hit
CGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGA	65877	0.18957466262417988	No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG	64727	0.18626530029714908	No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC	63111	0.181614926801078	No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC	58758	0.16908827096667364	No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA	58516	0.16839186602481152	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	57786	0.16629114037373982	No Hit
CCAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTC	57599	0.1657530092823009	No Hit
CGCAGGATATTTTATTGAGACAAGGTGGGTCAACGGGAAGCTGAGCAGTG	56377	0.16223645209653428	No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA	55673	0.16021054681111718	No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA	55092	0.1585385994093738	No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG	54100	0.15568391468901333	No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT	52958	0.1523975740129532	No Hit
CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT	52580	0.15130980100459004	No Hit
GTGAAATAAATGCTTTTTATTTGTCAGAAGACAGATTTTCAAATGTCTTT	52085	0.1498853363507811	No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC	51927	0.1494306587441108	No Hit
CAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCA	50306	0.14476589671618306	No Hit
CGAACGTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGA	49518	0.14249826409557415	No Hit
CGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAG	48523	0.1396349462560997	No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG	48037	0.13823638095963275	No Hit
AACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCC	47958	0.1380090421562976	No Hit
CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT	47517	0.13673997364654059	No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG	47482	0.136639253923544	No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT	45538	0.13104499273767634	No Hit
AGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATAATAAAT	45511	0.13096729466565038	No Hit
AATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAA	45465	0.13083492017256915	No Hit
CAAAGACCAAGAGGTACAGGTGCAAGGGAGAGAAGAAGGGCATGGCCAGA	43914	0.1263715975906346	No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA	42017	0.12091258860421947	No Hit
GTTTATTTTACACTGGGCGAATTGCTTCTGTACAATAGAAAGCAGTCTGT	41208	0.11858452414981258	No Hit
GAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGT	40742	0.11724351298077228	No Hit
TATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAAT	39749	0.1143859505540405	No Hit
TTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGAT	39400	0.11338163103044593	No Hit
CTCAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCT	38405	0.11051831319097147	No Hit
GTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGAGGTTATTCCTC	38078	0.1095773032075462	No Hit
GTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCT	37144	0.10688952545672294	No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT	36556	0.10519743411038024	No Hit
CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT	35759	0.10290390213242935	No Hit
CTCAGGCTTTATTCAAAGACCAAGAGGTACAGGTGCAAGGGAGAGAAGAA	35319	0.10163771132904365	No Hit
CACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTT	35056	0.10088087455338357	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.75541274266223E-6	0.0	0.0	0.0	0.0
2	5.75541274266223E-6	0.0	0.0	0.0	0.0
3	8.633119113993346E-6	0.0	0.0	0.0	0.0
4	8.633119113993346E-6	0.0	0.0	0.0	0.0
5	8.633119113993346E-6	0.0	0.0	0.0	0.0
6	8.633119113993346E-6	0.0	0.0	0.0	0.0
7	8.633119113993346E-6	0.0	0.0	0.0	0.0
8	8.633119113993346E-6	0.0	0.0	0.0	0.0
9	8.633119113993346E-6	0.0	0.0	0.0	0.0
10-11	1.4388531856655575E-5	0.0	0.0	0.0	0.0
12-13	2.1582797784983364E-5	0.0	0.0	2.877706371331115E-6	0.0
14-15	3.3093623270307825E-5	0.0	0.0	2.877706371331115E-6	0.0
16-17	4.316559556996673E-5	1.4388531856655574E-6	0.0	2.877706371331115E-6	0.0
18-19	6.330954016928453E-5	2.877706371331115E-6	0.0	2.877706371331115E-6	0.0
20-21	8.633119113993345E-5	2.877706371331115E-6	0.0	2.877706371331115E-6	0.0
22-23	1.381299058238935E-4	2.877706371331115E-6	0.0	2.877706371331115E-6	0.0
24-25	2.446050415631448E-4	2.877706371331115E-6	0.0	2.877706371331115E-6	0.0
26-27	3.5539673685939274E-4	2.877706371331115E-6	0.0	2.877706371331115E-6	0.0
28-29	5.625915955952329E-4	2.877706371331115E-6	0.0	2.877706371331115E-6	0.0
30-31	9.755424598812479E-4	2.877706371331115E-6	0.0	2.877706371331115E-6	0.0
32-33	0.0017741059779256324	2.877706371331115E-6	0.0	2.877706371331115E-6	0.0
34-35	0.0031136782937802665	2.877706371331115E-6	0.0	2.877706371331115E-6	0.0
36-37	0.004969798903288835	2.877706371331115E-6	0.0	2.877706371331115E-6	0.0
38-39	0.008224484809264326	2.877706371331115E-6	0.0	2.877706371331115E-6	0.0
40-41	0.012310827856554511	2.877706371331115E-6	0.0	2.877706371331115E-6	0.0
42-43	0.017018755480052217	2.877706371331115E-6	0.0	2.877706371331115E-6	0.0
44-45	0.023154025463730153	2.877706371331115E-6	0.0	5.75541274266223E-6	0.0
46-47	0.030270593320032	2.877706371331115E-6	0.0	5.75541274266223E-6	0.0
48-49	0.03849795583566766	2.877706371331115E-6	0.0	5.75541274266223E-6	0.0
50-51	0.0479540989718617	2.877706371331115E-6	0.0	5.75541274266223E-6	0.0
52-53	0.06015269627993429	2.877706371331115E-6	0.0	5.75541274266223E-6	0.0
54-55	0.0754174897266602	2.877706371331115E-6	0.0	5.75541274266223E-6	0.0
56-57	0.0928420018050701	2.877706371331115E-6	0.0	5.75541274266223E-6	0.0
58-59	0.1123384624708384	2.877706371331115E-6	0.0	8.633119113993346E-6	0.0
60-61	0.13451406776831598	2.877706371331115E-6	0.0	8.633119113993346E-6	0.0
62-63	0.1620220629718701	2.877706371331115E-6	0.0	8.633119113993346E-6	0.0
64-65	0.1954221619707247	2.877706371331115E-6	0.0	8.633119113993346E-6	0.0
66-67	0.23496904177874256	2.877706371331115E-6	0.0	8.633119113993346E-6	1.4388531856655574E-6
68-69	0.28275191722151005	4.316559556996672E-6	0.0	8.633119113993346E-6	2.877706371331115E-6
70-71	0.33664128558424217	5.75541274266223E-6	0.0	8.633119113993346E-6	2.877706371331115E-6
72-73	0.4004947122577083	5.75541274266223E-6	0.0	8.633119113993346E-6	2.877706371331115E-6
74-75	0.48138559950264026	5.75541274266223E-6	0.0	8.633119113993346E-6	2.877706371331115E-6
76-77	0.5752405539504188	5.75541274266223E-6	0.0	8.633119113993346E-6	2.877706371331115E-6
78-79	0.6826854767368087	5.75541274266223E-6	0.0	8.633119113993346E-6	2.877706371331115E-6
80-81	0.8083621082387669	8.633119113993346E-6	0.0	8.633119113993346E-6	2.877706371331115E-6
82-83	0.9536992296696591	8.633119113993346E-6	0.0	8.633119113993346E-6	2.877706371331115E-6
84-85	1.1519660043884445	8.633119113993346E-6	0.0	8.633119113993346E-6	2.877706371331115E-6
86-87	1.368668804975163	8.633119113993346E-6	0.0	1.0071972299658903E-5	2.877706371331115E-6
88-89	1.6183875307601632	8.633119113993346E-6	0.0	1.151082548532446E-5	2.877706371331115E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	52675	0.0	88.87019	1
GCGTGTT	58205	0.0	79.47954	2
GTAAACG	20215	0.0	62.10972	1
TAAACGC	21685	0.0	57.898796	2
CGTGTTT	79385	0.0	57.877098	3
CGAACGT	10180	0.0	51.451683	1
TCGAACG	4335	0.0	46.11694	2
GTGTTTT	102640	0.0	43.63264	4
GTTTTTT	356640	0.0	38.98904	1
GTCGAAC	5275	0.0	38.619503	1
AAACGCT	32945	0.0	38.353474	3
CGCAGGA	16185	0.0	36.02944	1
GAACGTT	14975	0.0	35.45214	2
TAGATAT	37355	0.0	34.537445	3
CGGACTT	4175	0.0	34.34965	1
GTAGATA	37430	0.0	34.279385	2
ACGTTTA	16165	0.0	34.013958	4
ACGCTTC	37475	0.0	33.562916	5
AGATATA	38800	0.0	33.481796	4
ATCAATA	57540	0.0	33.156178	4
>>END_MODULE