FastQCFastQC Report
Fri 27 May 2016
SRR2032139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2032139_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34749897
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC1756420.5054461024733398No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT1215760.34986002980095166No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT826640.2378827194797153No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC663600.1909645948015328No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA649840.18700487083458117No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA643810.1852696138926685No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC601730.1731602254821072No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG463330.13333276930288454No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC459260.13216154280975279No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC442490.1273356292250305No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA432040.12432842606698949No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT428880.12341907085364887No Hit
CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT400160.11515429815518591No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT354760.10208951122934265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA403950.060.6667481
CCGAAAC544850.045.0969282
CCAAACG568200.043.1350758
CCCGATC20600.043.0574231
TACGCAT124900.039.6339239
ACAATTT492050.038.51363426-27
CTACGCA131900.037.7728848
CGAAACC648350.037.6370053
CGGCAAT172700.037.3814281
ACTCGTC493500.037.28327642-43
TCGTACC74300.036.3549272
CTAACGA495200.036.15236394-95
CTCGTAC77050.036.137511
TCGTCTA510950.035.99646444-45
TCCACTC952100.035.8785742
CTCCACT978350.035.6242071
CGTACCA77300.035.003133
CAAACGA704350.034.9267859
CTCTACG141900.034.7108546
CTCCGAT23400.034.1928831
Produced by FastQC (version 0.11.5)