##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2032139_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 34749897 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.81029702044872 34.0 31.0 34.0 31.0 34.0 2 32.95270190297255 34.0 31.0 34.0 31.0 34.0 3 32.98606752704907 34.0 31.0 34.0 31.0 34.0 4 36.38023160183755 37.0 37.0 37.0 35.0 37.0 5 36.35015211699764 37.0 37.0 37.0 35.0 37.0 6 36.264603920984285 37.0 37.0 37.0 35.0 37.0 7 36.23091702976846 37.0 37.0 37.0 35.0 37.0 8 36.23441375955733 37.0 37.0 37.0 35.0 37.0 9 38.05695110405651 39.0 39.0 39.0 35.0 39.0 10-11 38.114486785385296 39.0 39.0 39.0 37.0 39.0 12-13 38.028441408042156 39.0 39.0 39.0 36.0 39.0 14-15 39.53627615068902 41.0 40.0 41.0 37.0 41.0 16-17 39.50082626144187 41.0 39.0 41.0 37.0 41.0 18-19 39.47255140065595 41.0 39.5 41.0 36.5 41.0 20-21 39.49707504169005 41.0 39.0 41.0 37.0 41.0 22-23 39.48761761797452 41.0 39.0 41.0 37.0 41.0 24-25 39.51265294397851 41.0 39.0 41.0 37.0 41.0 26-27 39.48402747783684 41.0 39.0 41.0 37.0 41.0 28-29 29.66941910647965 21.5 20.5 41.0 19.5 41.0 30-31 30.680290951653756 26.5 23.0 41.0 21.0 41.0 32-33 37.68740087200834 38.5 37.0 40.5 34.0 41.0 34-35 38.9516506768351 40.0 38.5 41.0 35.5 41.0 36-37 39.200450651695455 41.0 39.0 41.0 36.0 41.0 38-39 39.212469565017706 41.0 39.0 41.0 35.0 41.0 40-41 39.17082017538066 41.0 39.0 41.0 35.0 41.0 42-43 39.126150057365635 41.0 39.0 41.0 35.0 41.0 44-45 39.076169837280375 41.0 39.0 41.0 35.0 41.0 46-47 39.00341154968028 41.0 39.0 41.0 35.0 41.0 48-49 38.925449692699814 41.0 39.0 41.0 35.0 41.0 50-51 38.878159379868094 40.5 38.5 41.0 35.0 41.0 52-53 38.76977225285013 40.0 38.0 41.0 35.0 41.0 54-55 38.63512074582552 40.0 38.0 41.0 35.0 41.0 56-57 38.47629630384228 40.0 38.0 41.0 34.0 41.0 58-59 38.298310639597005 40.0 37.0 41.0 34.0 41.0 60-61 38.06130933280176 39.5 37.0 41.0 34.0 41.0 62-63 37.75141080274281 39.0 36.0 41.0 34.0 41.0 64-65 37.367520830349505 39.0 36.0 41.0 33.0 41.0 66-67 36.98545502163647 38.0 35.0 40.0 33.0 41.0 68-69 36.51822757920692 37.0 35.0 39.5 32.5 41.0 70-71 36.160058603914706 37.0 35.0 39.0 32.0 41.0 72-73 35.83612518333507 36.0 35.0 39.0 32.0 41.0 74-75 35.48484710616552 36.0 35.0 38.5 32.0 40.0 76-77 35.16891703304904 35.0 35.0 37.0 32.0 39.0 78-79 34.81760281188747 35.0 34.5 37.0 31.5 39.0 80-81 34.44649116513928 35.0 34.0 36.0 31.0 39.0 82-83 34.210060910396365 35.0 34.0 36.0 31.0 37.0 84-85 34.00046627476334 35.0 34.0 36.0 31.0 37.0 86-87 33.75242509351898 35.0 34.0 35.0 30.5 36.5 88-89 33.6292016203674 35.0 34.0 35.0 31.0 36.0 90-91 33.60648253144463 35.0 34.0 35.0 31.0 36.0 92-93 33.62206652008206 35.0 34.0 35.0 31.0 36.0 94-95 33.53230075761088 35.0 34.0 35.0 31.0 36.0 96-97 33.47127857673938 35.0 34.0 35.0 31.0 36.0 98-99 33.35781399582278 35.0 34.0 35.0 31.0 36.0 100-101 33.08719445988574 35.0 34.0 35.0 30.5 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09890129141743387 1101 2 0.08586231333634942 1101 3 0.08320329407902705 1101 4 0.0772824572672306 1101 5 0.07114549329927655 1101 6 0.0787761984602966 1101 7 0.07080119906213866 1101 8 0.044267631616783376 1101 9 0.09360661824466376 1101 10-11 0.05922105863260896 1101 12-13 0.0763986197901545 1101 14-15 0.12325949990462703 1101 16-17 0.06033432439304676 1101 18-19 0.12227149182992036 1101 20-21 0.10624560530300897 1101 22-23 0.12524526949380288 1101 24-25 0.12179434045660287 1101 26-27 0.11379949432621572 1101 28-29 9.44677253995146 1101 30-31 8.253464025481492 1101 32-33 1.4637401956325036 1101 34-35 0.3471223732472808 1101 36-37 0.1463317852981021 1101 38-39 0.134754658625738 1101 40-41 0.09468162232710142 1101 42-43 0.10434829063681406 1101 44-45 0.09321846673159939 1101 46-47 0.12692492012218537 1101 48-49 0.10638924082972778 1101 50-51 0.13505046696126755 1101 52-53 0.1173760860893509 1101 54-55 0.11450104202348399 1101 56-57 0.12681259984370996 1101 58-59 0.18275139844085686 1101 60-61 0.1701557012046493 1101 62-63 0.2237895410401194 1101 64-65 0.2770234776238425 1101 66-67 0.283284054945419 1101 68-69 0.2889583810065304 1101 70-71 0.29343828109963255 1101 72-73 0.26489818114011143 1101 74-75 0.2588484540740268 1101 76-77 0.2878946040670911 1101 78-79 0.30026045499997167 1101 80-81 0.3192105769670306 1101 82-83 0.26924292050845366 1101 84-85 0.2375284123648811 1101 86-87 0.2513455254178254 1101 88-89 0.23192532127498566 1101 90-91 0.18031776026779767 1101 92-93 0.13463574146749124 1101 94-95 0.11979494199470508 1101 96-97 0.07380981708320888 1101 98-99 0.10661045141616654 1101 100-101 0.05920582866039581 1102 1 0.030340941387024145 1102 2 0.0538412203346752 1102 3 0.0517890020557914 1102 4 0.04853462040464507 1102 5 0.04505102639413394 1102 6 0.0839565975971297 1102 7 0.07007392410757518 1102 8 0.06361407277184838 1102 9 0.07963185616046076 1102 10-11 0.05601819187611312 1102 12-13 0.08687463628095315 1102 14-15 0.10680762344557593 1102 16-17 0.08904234652691656 1102 18-19 0.142772214222056 1102 20-21 0.11753569465751923 1102 22-23 0.12509852402759236 1102 24-25 0.099557947338063 1102 26-27 0.09626246838957542 1102 28-29 9.438269423957841 1102 30-31 8.275108392225263 1102 32-33 1.4822500514830494 1102 34-35 0.3463404440061666 1102 36-37 0.14095929834753207 1102 38-39 0.0924872848917957 1102 40-41 0.10036070476136416 1102 42-43 0.12858404507708343 1102 44-45 0.09996930169977247 1102 46-47 0.09882898453753342 1102 48-49 0.1083433602953221 1102 50-51 0.08080324557271723 1102 52-53 0.1038958381931252 1102 54-55 0.11174031152547315 1102 56-57 0.12304260765000663 1102 58-59 0.1546696262195013 1102 60-61 0.13808489596065954 1102 62-63 0.20898094660221034 1102 64-65 0.23969314367595018 1102 66-67 0.2534067663140007 1102 68-69 0.18632384416245173 1102 70-71 0.2497880634935612 1102 72-73 0.24832312809854074 1102 74-75 0.24990007355846444 1102 76-77 0.25973972070612916 1102 78-79 0.27171986573888773 1102 80-81 0.2550217992947168 1102 82-83 0.2245216435419053 1102 84-85 0.2104429364754523 1102 86-87 0.18789156824127673 1102 88-89 0.16685495927975325 1102 90-91 0.14062170043329303 1102 92-93 0.13531037867591778 1102 94-95 0.13023631284442416 1102 96-97 0.11218766500193311 1102 98-99 0.09500415612621538 1102 100-101 0.04018451012682078 1103 1 0.014745753466755218 1103 2 0.02214884266511774 1103 3 0.03252190158794832 1103 4 0.02610990389536738 1103 5 0.015763064763859802 1103 6 0.03709323793684405 1103 7 0.03458607947661818 1103 8 0.010995492562166476 1103 9 0.029603858678811434 1103 10-11 0.012439038142332492 1103 12-13 0.03446779253070531 1103 14-15 0.07271083718046611 1103 16-17 0.059462936171236436 1103 18-19 0.09336841548354613 1103 20-21 0.0550432178112672 1103 22-23 0.040083866893525055 1103 24-25 0.05948021084731181 1103 26-27 0.011179204434206724 1103 28-29 9.390407481235776 1103 30-31 8.203405315632253 1103 32-33 1.3522351556484296 1103 34-35 0.2949621766177657 1103 36-37 0.05150853389544352 1103 38-39 0.04598849598291821 1103 40-41 0.0349073008502927 1103 42-43 0.05373439285268233 1103 44-45 0.050000196787095774 1103 46-47 0.053332542862804644 1103 48-49 0.0493814747910335 1103 50-51 0.028464418435021344 1103 52-53 0.04330082136876001 1103 54-55 0.037817321714307184 1103 56-57 0.06272187511304139 1103 58-59 0.09717826237414329 1103 60-61 0.0447468885553306 1103 62-63 0.10658213807496963 1103 64-65 0.1330538106311323 1103 66-67 0.18022193176460632 1103 68-69 0.2078202148021333 1103 70-71 0.22567710216544157 1103 72-73 0.19763047779446197 1103 74-75 0.21143076933662996 1103 76-77 0.1906469957980974 1103 78-79 0.18345745810800906 1103 80-81 0.15971127055619405 1103 82-83 0.17593038438959496 1103 84-85 0.11878318031161683 1103 86-87 0.12992725560742002 1103 88-89 0.14108260752268365 1103 90-91 0.10428544100494008 1103 92-93 0.07479377146627542 1103 94-95 0.038670300361403065 1103 96-97 0.023805546268121702 1103 98-99 -0.0017096369182709736 1103 100-101 -0.11757051107502292 1104 1 -5.659974678948743E-4 1104 2 0.0059974393793140734 1104 3 7.910833147732887E-4 1104 4 0.008342214085210742 1104 5 0.007901591768344929 1104 6 0.052617459324387994 1104 7 0.03545229274022432 1104 8 0.019144715221209196 1104 9 -0.003045520112465283 1104 10-11 0.00888906446783011 1104 12-13 0.017829753975966867 1104 14-15 0.021441254836084056 1104 16-17 0.03440691758495973 1104 18-19 0.08632378023637699 1104 20-21 0.014608275841887064 1104 22-23 0.021832370738650297 1104 24-25 0.02049879031756774 1104 26-27 0.010886890816344419 1104 28-29 9.392679817243923 1104 30-31 8.239283915148235 1104 32-33 1.3289395361880452 1104 34-35 0.21528405544761853 1104 36-37 -0.0191479628290665 1104 38-39 0.009614276295771162 1104 40-41 -0.04901259815500225 1104 42-43 -0.013889611403463675 1104 44-45 0.0024248929255605844 1104 46-47 0.01519497088031585 1104 48-49 0.012013744270859661 1104 50-51 0.020590633866362396 1104 52-53 -0.0033524423423827443 1104 54-55 0.05267641012370916 1104 56-57 0.0774401260941815 1104 58-59 0.0898399039287483 1104 60-61 0.07828199113212975 1104 62-63 0.1250563494584398 1104 64-65 0.16194302478344724 1104 66-67 0.16232279126315063 1104 68-69 0.2182607427874288 1104 70-71 0.24038590151111094 1104 72-73 0.17810233314906299 1104 74-75 0.17047071704431005 1104 76-77 0.2100075186910786 1104 78-79 0.18939354962281385 1104 80-81 0.21974781386291653 1104 82-83 0.19855498080541878 1104 84-85 0.1568741231919759 1104 86-87 0.1511846819781013 1104 88-89 0.10674871166398958 1104 90-91 0.05141180119825606 1104 92-93 0.03090534727434857 1104 94-95 0.02171792328028488 1104 96-97 0.0043185687206062084 1104 98-99 0.040654480893195455 1104 100-101 -0.0317621256801246 1105 1 0.009770862100353384 1105 2 0.0018793939653249936 1105 3 0.0013245916798894086 1105 4 0.0177580565491553 1105 5 -0.007812579519416829 1105 6 0.022056326101967727 1105 7 -0.011292711697699076 1105 8 -0.008872035674293954 1105 9 -0.0090202114354625 1105 10-11 -0.008100912592389875 1105 12-13 -0.010861864925644227 1105 14-15 0.0020101746915131002 1105 16-17 -0.03470740918903914 1105 18-19 0.06208651528678644 1105 20-21 0.019633377846396627 1105 22-23 0.04229970184988474 1105 24-25 0.02309553971073086 1105 26-27 0.0017455679913140898 1105 28-29 9.378193412324471 1105 30-31 8.179903055516618 1105 32-33 1.399661613537262 1105 34-35 0.22924513893530474 1105 36-37 0.02073779514577012 1105 38-39 0.02504752392431442 1105 40-41 -0.03023395521725547 1105 42-43 -0.02132591405565165 1105 44-45 -0.01851978525718323 1105 46-47 0.0014448770867403482 1105 48-49 -0.03824903731826623 1105 50-51 -0.017130429926595525 1105 52-53 -0.03631317989484728 1105 54-55 -0.008509078241964119 1105 56-57 0.0071172571617736935 1105 58-59 0.014356528605631524 1105 60-61 0.04037393622657959 1105 62-63 0.07290635375823484 1105 64-65 0.0970611358271114 1105 66-67 0.1396390566321628 1105 68-69 0.13189229867555952 1105 70-71 0.17949418129225592 1105 72-73 0.12231296745976294 1105 74-75 0.12967486242438042 1105 76-77 0.1475344345988745 1105 78-79 0.16530800031201665 1105 80-81 0.13412977559048755 1105 82-83 0.07577342026458211 1105 84-85 0.1059081152050041 1105 86-87 0.07846987959669605 1105 88-89 0.059219350154243955 1105 90-91 0.007881477011913773 1105 92-93 0.010395960423700501 1105 94-95 0.01730379617531952 1105 96-97 -0.004100422743832155 1105 98-99 -0.031743457898137706 1105 100-101 -0.0981801690759383 1106 1 -0.05623816840750351 1106 2 -0.05013505744081925 1106 3 -0.04465761718427075 1106 4 -0.004216142030543324 1106 5 -0.04547104329424201 1106 6 -0.038979421961364835 1106 7 -0.04075527775356136 1106 8 -0.0600582449535807 1106 9 -0.05393215935278306 1106 10-11 -0.04759265595994577 1106 12-13 -0.038667864000174745 1106 14-15 -0.05720130797108425 1106 16-17 -0.11739625307824753 1106 18-19 -0.044491094455032965 1106 20-21 -0.10347675031272985 1106 22-23 -0.11844402227226425 1106 24-25 -0.1364160743096292 1106 26-27 -0.14284621483745497 1106 28-29 9.25466071718079 1106 30-31 8.142724357444976 1106 32-33 1.3296601474681466 1106 34-35 0.11994145571917159 1106 36-37 -0.08859501883078735 1106 38-39 -0.12885243567932747 1106 40-41 -0.13499234722702624 1106 42-43 -0.16165214920885518 1106 44-45 -0.14617677901544113 1106 46-47 -0.12804290764874793 1106 48-49 -0.13140860021093914 1106 50-51 -0.10383409499350194 1106 52-53 -0.08589865416313103 1106 54-55 -0.08168480300012249 1106 56-57 -0.03188891856347453 1106 58-59 -6.107099132250937E-4 1106 60-61 0.005730547062462676 1106 62-63 -0.03237695544110153 1106 64-65 0.04593951645630767 1106 66-67 0.06187559864832792 1106 68-69 0.06719613821716308 1106 70-71 0.12577632696670804 1106 72-73 0.08928215821391916 1106 74-75 0.08981928152697094 1106 76-77 0.1072594903484898 1106 78-79 0.11902465319775501 1106 80-81 0.12262090294398575 1106 82-83 0.09076036684988509 1106 84-85 0.08922860797085974 1106 86-87 0.06242278868000284 1106 88-89 0.03224755326367301 1106 90-91 0.03374078298315908 1106 92-93 0.002733021348902298 1106 94-95 -0.020503642612887063 1106 96-97 -0.03984429484936669 1106 98-99 -0.02575610636976222 1106 100-101 -0.13991692298687752 1107 1 -0.08624582381295198 1107 2 -0.06455347652293142 1107 3 -0.06700189474279483 1107 4 -0.020693558261257294 1107 5 -0.011257568286495712 1107 6 -0.005540854596894462 1107 7 -0.015619919416714367 1107 8 -0.05054486264273095 1107 9 -0.044194692920932255 1107 10-11 -0.03863541667788439 1107 12-13 -0.04523268773623812 1107 14-15 -0.05339361352493199 1107 16-17 -0.0734313288842614 1107 18-19 -0.022653327810125745 1107 20-21 -0.028286956577481703 1107 22-23 -0.0649407462278262 1107 24-25 -0.07591791554124683 1107 26-27 -0.057602195031627446 1107 28-29 9.306153537205418 1107 30-31 8.13331374724131 1107 32-33 1.3061205450194038 1107 34-35 0.16673203676146642 1107 36-37 -0.1266382304706113 1107 38-39 -0.07193205112714196 1107 40-41 -0.11274036003662502 1107 42-43 -0.11091468105042424 1107 44-45 -0.1236078164086365 1107 46-47 -0.0931941481396592 1107 48-49 -0.11061935000805789 1107 50-51 -0.07746153229933128 1107 52-53 -0.08916424015764335 1107 54-55 -0.07361219060678081 1107 56-57 -0.08686331550603654 1107 58-59 -0.03905555241927061 1107 60-61 -0.05667803373791003 1107 62-63 -0.029320836481005585 1107 64-65 -0.023054098954617075 1107 66-67 0.02374363015778158 1107 68-69 0.05827923726207729 1107 70-71 0.052543727376459515 1107 72-73 0.0365595784325663 1107 74-75 0.08506847053887157 1107 76-77 0.044327736083900504 1107 78-79 0.07379366099696938 1107 80-81 0.10361949842850748 1107 82-83 0.03656881853013516 1107 84-85 -0.007779099960465885 1107 86-87 -0.042952303267604464 1107 88-89 -0.03312995552388287 1107 90-91 -0.04501386087875403 1107 92-93 -0.09594485127964703 1107 94-95 -0.06749264287271473 1107 96-97 -0.09062955911677761 1107 98-99 -0.07930654671893222 1107 100-101 -0.148971581284151 1108 1 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2214 64-65 -0.15870242820118108 2214 66-67 -0.18164154225817697 2214 68-69 -0.13713561606163438 2214 70-71 -0.19059521143973512 2214 72-73 -0.1819817554983345 2214 74-75 -0.20310695049524696 2214 76-77 -0.2415296808509595 2214 78-79 -0.23390637143024406 2214 80-81 -0.22310373380800996 2214 82-83 -0.19750015385609743 2214 84-85 -0.15037333324284674 2214 86-87 -0.11981561041912414 2214 88-89 -0.08844597134390852 2214 90-91 -0.04707253608079043 2214 92-93 -0.05965088885657366 2214 94-95 -0.032493892962428106 2214 96-97 -0.0036978687599358295 2214 98-99 0.027619975861135515 2214 100-101 -0.03830745426686377 2215 1 -0.07757532428792757 2215 2 -0.06702587484197409 2215 3 -0.08921317185458832 2215 4 -0.06639306857752558 2215 5 -0.0192786595060781 2215 6 -0.2049392773530201 2215 7 -0.0832152530135275 2215 8 -0.06932309107131829 2215 9 -0.06324103516087831 2215 10-11 -0.06606859877022231 2215 12-13 -0.03430889796536718 2215 14-15 -0.14521276360209612 2215 16-17 -0.09749043785233624 2215 18-19 -0.0758577203612063 2215 20-21 -0.08125716079891987 2215 22-23 -0.0761524445721733 2215 24-25 -0.10158012454326126 2215 26-27 -0.10912786634054328 2215 28-29 -9.366605901753555 2215 30-31 -8.260785344890095 2215 32-33 -1.5747114607990227 2215 34-35 -0.4302708208467294 2215 36-37 -0.18500692966669163 2215 38-39 -0.12511723269955866 2215 40-41 -0.14515593726579823 2215 42-43 -0.14798051364773102 2215 44-45 -0.14121036593240888 2215 46-47 -0.2126328679118501 2215 48-49 -0.16201360699916023 2215 50-51 -0.1494925782355665 2215 52-53 -0.14680680215242603 2215 54-55 -0.17199768617728495 2215 56-57 -0.21813228766867354 2215 58-59 -0.2480228382921723 2215 60-61 -0.22059764405368298 2215 62-63 -0.20288753057759124 2215 64-65 -0.24297578507482598 2215 66-67 -0.3377184644463682 2215 68-69 -0.2705919521654394 2215 70-71 -0.2999062614783341 2215 72-73 -0.3057538039074501 2215 74-75 -0.33876003764247287 2215 76-77 -0.31977589162631403 2215 78-79 -0.3172120450479312 2215 80-81 -0.31518122991521835 2215 82-83 -0.32321055066189786 2215 84-85 -0.3165426657866348 2215 86-87 -0.30213258186470426 2215 88-89 -0.23666943780654748 2215 90-91 -0.18599927551110795 2215 92-93 -0.1585597539063457 2215 94-95 -0.16322433877480336 2215 96-97 -0.16259186064017683 2215 98-99 -0.15173504886652012 2215 100-101 -0.23364201438899812 2216 1 -0.1945798644077925 2216 2 -0.15073891508130544 2216 3 -0.14366257484000045 2216 4 -0.11526004964421332 2216 5 -0.0928458311442526 2216 6 -0.12007350670621975 2216 7 -0.11332104352683103 2216 8 -0.1050716716407365 2216 9 -0.1640746832806741 2216 10-11 -0.1725666712127989 2216 12-13 -0.16440233761828438 2216 14-15 -0.23332348134590575 2216 16-17 -0.24050815306492979 2216 18-19 -0.2224673453143069 2216 20-21 -0.2747037583860603 2216 22-23 -0.252580009146925 2216 24-25 -0.25283681308238926 2216 26-27 -0.300441994708855 2216 28-29 -9.470016432183918 2216 30-31 -8.443020003933938 2216 32-33 -1.8778007328358086 2216 34-35 -0.7223570055281527 2216 36-37 -0.3721764393847309 2216 38-39 -0.34985382261317 2216 40-41 -0.3122881254778065 2216 42-43 -0.32630245443989025 2216 44-45 -0.295592439685052 2216 46-47 -0.2818798391538877 2216 48-49 -0.3740018328407828 2216 50-51 -0.36743316834498785 2216 52-53 -0.3431121493971574 2216 54-55 -0.3863858693167259 2216 56-57 -0.39572109227029983 2216 58-59 -0.42241911225584516 2216 60-61 -0.4237220458790887 2216 62-63 -0.40321692282881827 2216 64-65 -0.44563445665868784 2216 66-67 -0.45750770784217565 2216 68-69 -0.4257149033712224 2216 70-71 -0.4678168911027498 2216 72-73 -0.49179491433561395 2216 74-75 -0.5260668812281324 2216 76-77 -0.5052307173101838 2216 78-79 -0.5027338509459582 2216 80-81 -0.4988584690718696 2216 82-83 -0.48601502012007813 2216 84-85 -0.42635995203350063 2216 86-87 -0.410352441433524 2216 88-89 -0.3930340765388891 2216 90-91 -0.3910404079854075 2216 92-93 -0.4232247803439293 2216 94-95 -0.3988857870046445 2216 96-97 -0.4382915411367847 2216 98-99 -0.41253028661281377 2216 100-101 -0.4865531372007226 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1815.0 3 735.0 4 893.0 5 1316.0 6 2325.0 7 4247.0 8 6404.0 9 7510.0 10 9486.0 11 13190.0 12 16060.0 13 16354.0 14 18352.0 15 21560.0 16 23810.0 17 25420.0 18 27262.0 19 30073.0 20 33695.0 21 39164.0 22 47166.0 23 59069.0 24 74470.0 25 95847.0 26 124469.0 27 160911.0 28 208455.0 29 266917.0 30 343965.0 31 452300.0 32 617749.0 33 890298.0 34 1440745.0 35 2504228.0 36 5013925.0 37 1.0791434E7 38 9272785.0 39 2077531.0 40 7962.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.420023456914482 10.839436669022568 6.78247461437166 57.958065259691296 2 18.008077324484034 15.332979778084368 44.59616010682974 22.062782790601855 3 21.465266501365456 20.73512333000584 23.19364860275701 34.60596156587169 4 22.141015497019605 30.940360870496725 20.638899716356324 26.279723916127345 5 21.135373630240746 36.15450546176548 24.270250703635444 18.43987020435833 6 17.702084153889032 36.78343030286279 25.804639796202757 19.709845747045424 7 15.157098200598531 24.275404546334343 41.49916145956626 19.068335793500864 8 16.938775386873807 22.331715112709542 32.76166257413655 27.9678469262801 9 16.581939796828753 23.11569153715765 35.78527729161326 24.517091374400334 10-11 21.509062314630746 34.21235896037332 23.952542938472597 20.326035786523338 12-13 21.407083595096697 27.844430733132818 27.35594439315892 23.39254127861156 14-15 20.95874701441561 30.12058568116044 26.692784154151596 22.22788315027236 16-17 21.864430274426425 27.252362791176044 27.886258195240117 22.996948739157414 18-19 21.302301989556977 28.238118230969146 26.384141512707217 24.07543826676666 20-21 21.59489422371525 27.384532967104906 26.51348865868581 24.50708415049403 22-23 21.12076332197474 29.33168118455142 26.591750761160533 22.955804732313307 24-25 20.71382110859206 29.851521574294164 26.531800943179775 22.902856373934 26-27 20.576123817950155 30.702285058357003 26.793425751268927 21.928165372423912 28-29 20.83152914242767 29.105325391800037 26.342239728183554 23.720905737588748 30-31 20.300235298893483 28.531435288652567 27.78493389361602 23.383395518837922 32-33 20.980434328077948 27.765409921178748 28.964490146735088 22.289665604008217 34-35 20.53878749626222 30.12646915183662 26.076304629046813 23.258438722854343 36-37 20.487520484988053 29.704047805149735 26.500799571742988 23.307632138119217 38-39 20.195864306363784 28.9598684835484 26.786392040385014 24.057875169702804 40-41 20.309349928656957 29.44628255735897 26.02143948888896 24.22292802509512 42-43 19.911658127454 29.117023578149293 27.620717949981188 23.35060034441552 44-45 19.669248598877434 28.105633080348774 27.245110513537984 24.980007807235808 46-47 20.07796310878274 29.301852319159394 27.167374050058335 23.452810521999535 48-49 19.776419768956437 28.46128867662543 27.313987434264913 24.448304120153217 50-51 19.479201621806247 29.129964039893412 27.66653811952306 23.724296218777283 52-53 21.582463971044287 28.557714861715993 26.67077257811728 23.18904858912244 54-55 20.723500273972036 28.36805818446023 26.76649228629368 24.14194925527405 56-57 21.676015639355935 28.50492662618434 26.16662613656993 23.652431597889795 58-59 19.78638526612036 30.64520881889233 26.735546870829573 22.83285904415774 60-61 19.772088820867584 30.733327065688858 26.145805554474016 23.348778558969542 62-63 20.71359759462151 29.63589921840077 27.12924864714079 22.521254539836928 64-65 21.604956124042612 28.925697247390403 26.176152407012893 23.293194221554096 66-67 21.936981833758306 28.7454842674013 26.168524903131267 23.149008995709128 68-69 20.04060841423967 29.721793310371837 25.953370571334762 24.284227704053734 70-71 19.997337803651465 30.758228398259398 25.69163075512188 23.552803042967263 72-73 19.538066866479063 29.942091624146155 26.960657428310963 23.559184081063812 74-75 19.75902982720707 28.545835971290185 28.16740766400384 23.527726537498907 76-77 18.999929920626116 29.293130796747803 27.40863455592341 24.298304726702675 78-79 21.072306306592292 30.643277844570694 26.066225872294346 22.21818997654267 80-81 19.518034351436107 30.09823390937963 26.095679160673996 24.288052578510264 82-83 19.918520939065388 30.24473647367154 25.53941495254297 24.2973276347201 84-85 19.657295621832148 29.7330357869161 26.3563292703822 24.253339320869554 86-87 20.191373268839786 30.167334162229675 26.76875508668569 22.872537482244848 88-89 19.24524923324459 29.796081668404973 27.417531865917645 23.54113723243279 90-91 19.864584321761324 31.57956455723074 26.657196753026096 21.898654367981838 92-93 20.860568667753 29.874670775985436 27.293630484438815 21.971130071822753 94-95 19.284966834452163 29.183047129004393 28.171403839698712 23.36058219684473 96-97 19.386221481535795 30.178137708336944 28.59345618843055 21.84218462169671 98-99 20.49267019068913 29.481556263732735 26.427790036129295 23.597983509448845 100-101 20.470707604190245 31.057902379276037 25.661475280935818 22.809914735597893 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 60.0 1 61.0 2 48.0 3 29.5 4 28.5 5 40.5 6 42.5 7 43.0 8 61.0 9 66.0 10 71.5 11 98.5 12 142.5 13 210.5 14 289.5 15 421.0 16 662.0 17 1128.0 18 1946.0 19 3490.5 20 9004.5 21 16029.0 22 24871.5 23 53474.5 24 88747.5 25 101233.5 26 120140.5 27 180732.5 28 266161.5 29 355670.5 30 435869.5 31 514070.5 32 618926.0 33 771496.5 34 916217.0 35 1105407.0 36 1295558.0 37 1532821.0 38 1804159.5 39 1767290.5 40 1734532.0 41 1752092.0 42 1445017.0 43 1189623.5 44 1179907.5 45 1162424.5 46 1122991.5 47 1096596.5 48 1141995.5 49 1179953.0 50 1064445.5 51 922608.5 52 887749.0 53 846851.5 54 809639.0 55 794129.0 56 711770.5 57 595492.0 58 503126.5 59 477101.0 60 429681.0 61 327267.5 62 240045.5 63 183391.5 64 153632.5 65 124045.5 66 82165.5 67 53465.0 68 30629.5 69 20739.0 70 37725.5 71 72853.0 72 129734.5 73 157261.5 74 78550.5 75 8238.5 76 5985.0 77 5501.5 78 1871.5 79 1039.0 80 420.0 81 214.5 82 207.5 83 168.0 84 99.5 85 50.5 86 37.0 87 18.0 88 5.0 89 2.5 90 2.0 91 4.5 92 3.5 93 2.0 94 1.0 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.03746198154198845 2 6.906495291194677E-5 3 0.0 4 8.97844387855308E-4 5 0.03071088239484566 6 0.04989079535976754 7 6.647501717774876E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.1654710803891016E-4 28-29 26.041281503654528 30-31 0.18849264502855936 32-33 0.057152687387821616 34-35 0.0 36-37 0.001928063268791847 38-39 5.064763213542763E-4 40-41 5.410087978102497E-4 42-43 3.1654770084642266E-5 44-45 2.3597192244915145E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.4388531856655576E-6 58-59 0.0 60-61 0.0 62-63 0.0012618742438286938 64-65 0.0 66-67 0.0015884939169747755 68-69 0.004125192083303153 70-71 0.0011338163103044594 72-73 0.004228789512671073 74-75 0.0045223155625468475 76-77 0.010307944222108054 78-79 0.010395714266433653 80-81 0.0014129538283235775 82-83 0.003142455357493578 84-85 0.009420171806552404 86-87 0.0013741047923106074 88-89 0.007211532166555774 90-91 0.011706509518574977 92-93 0.014755439419000291 94-95 0.016214436549265167 96-97 0.016833143419101356 98-99 3.4100820500273713E-4 100-101 0.04958575848440644 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.4749897E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.095539882044722 #Duplication Level Percentage of deduplicated Percentage of total 1 69.75306391653001 14.714785417459735 2 11.105273098279394 4.685434630915028 3 4.995915178702055 3.1617458369886537 4 2.882982142771661 2.432722590882493 5 1.9762274863587577 2.0844792877237084 6 1.4084049506313578 1.7826637683667823 7 1.0001688609658979 1.4769371466699739 8 0.8092887910607841 1.3657907174331625 9 0.6335262155272464 1.2028119791378296 >10 4.591060684366716 19.02591490802194 >50 0.48000953159817294 7.021319103869845 >100 0.3034561182313042 12.144134397071898 >500 0.030834779019544185 4.489488130326507 >1k 0.024937828843967648 10.566002945784987 >5k 0.0029490536050841088 4.165781018845856 >10k+ 0.0019013635085410683 9.679988120501738 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC 175642 0.5054461024733398 No Hit CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 121576 0.34986002980095166 No Hit CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT 82664 0.2378827194797153 No Hit CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC 66360 0.1909645948015328 No Hit CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA 64984 0.18700487083458117 No Hit CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 64381 0.1852696138926685 No Hit CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC 60173 0.1731602254821072 No Hit ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG 46333 0.13333276930288454 No Hit GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC 45926 0.13216154280975279 No Hit CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC 44249 0.1273356292250305 No Hit CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA 43204 0.12432842606698949 No Hit CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT 42888 0.12341907085364887 No Hit CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT 40016 0.11515429815518591 No Hit CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 35476 0.10208951122934265 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.75541274266223E-6 0.0 0.0 2.877706371331115E-6 2.877706371331115E-6 2 5.75541274266223E-6 0.0 0.0 2.877706371331115E-6 2.877706371331115E-6 3 8.633119113993346E-6 0.0 0.0 2.877706371331115E-6 2.877706371331115E-6 4 1.151082548532446E-5 0.0 0.0 2.877706371331115E-6 2.877706371331115E-6 5 1.151082548532446E-5 0.0 0.0 2.877706371331115E-6 2.877706371331115E-6 6 1.151082548532446E-5 0.0 0.0 2.877706371331115E-6 2.877706371331115E-6 7 1.4388531856655575E-5 0.0 0.0 5.75541274266223E-6 2.877706371331115E-6 8 1.4388531856655575E-5 0.0 0.0 1.151082548532446E-5 2.877706371331115E-6 9 1.4388531856655575E-5 0.0 0.0 1.726623822798669E-5 2.877706371331115E-6 10-11 2.0143944599317805E-5 0.0 0.0 2.302165097064892E-5 2.877706371331115E-6 12-13 2.733821052764559E-5 0.0 0.0 2.5899357341980036E-5 2.877706371331115E-6 14-15 4.172674238430117E-5 0.0 0.0 3.0215916898976708E-5 2.877706371331115E-6 16-17 5.323756786962562E-5 0.0 0.0 3.1654770084642266E-5 2.877706371331115E-6 18-19 6.906495291194675E-5 0.0 0.0 3.5971329641638935E-5 2.877706371331115E-6 20-21 7.625921884027455E-5 0.0 0.0 3.741018282730449E-5 2.877706371331115E-6 22-23 9.640316343959235E-5 0.0 0.0 3.741018282730449E-5 2.877706371331115E-6 24-25 1.3956875900955908E-4 0.0 0.0 3.741018282730449E-5 2.877706371331115E-6 26-27 1.8561206095085692E-4 0.0 0.0 4.6043301941297844E-5 2.877706371331115E-6 28-29 2.7482095846212147E-4 0.0 0.0 5.467642105529119E-5 2.877706371331115E-6 30-31 6.575559058491598E-4 0.0 0.0 5.467642105529119E-5 2.877706371331115E-6 32-33 0.0014805799280498586 0.0 0.0 5.467642105529119E-5 2.877706371331115E-6 34-35 0.0028287853630184863 0.0 0.0 5.75541274266223E-5 2.877706371331115E-6 36-37 0.004717999595797363 0.0 0.0 6.474839335495009E-5 2.877706371331115E-6 38-39 0.007997146005929169 0.0 0.0 6.762609972628121E-5 2.877706371331115E-6 40-41 0.012143920887017306 0.0 0.0 7.482036565460899E-5 2.877706371331115E-6 42-43 0.016902208372013304 0.0 0.0 8.345348476860234E-5 2.877706371331115E-6 44-45 0.02306050000666189 0.0 0.0 8.777004432559901E-5 2.877706371331115E-6 46-47 0.03017994556933507 0.0 0.0 1.0071972299658903E-4 2.877706371331115E-6 48-49 0.03843896285505537 0.0 0.0 1.0359742936792014E-4 2.877706371331115E-6 50-51 0.04786345122116477 0.0 0.0 1.0647513573925126E-4 2.877706371331115E-6 52-53 0.05999154472313976 0.0 0.0 1.0791398892491682E-4 2.877706371331115E-6 54-55 0.07519590633606771 0.0 0.0 1.151082548532446E-4 2.877706371331115E-6 56-57 0.09255135346156565 0.0 2.877706371331115E-6 1.1798596122457571E-4 2.877706371331115E-6 58-59 0.1117370218392302 0.0 2.877706371331115E-6 1.2661908033856906E-4 2.877706371331115E-6 60-61 0.13344068329180947 0.0 2.877706371331115E-6 1.3669105263822796E-4 2.877706371331115E-6 62-63 0.16028392832358612 0.0 2.877706371331115E-6 1.4388531856655574E-4 2.877706371331115E-6 64-65 0.1931574070564871 0.0 2.877706371331115E-6 1.4532417175222128E-4 2.877706371331115E-6 66-67 0.23188557940186125 0.0 2.877706371331115E-6 1.4676302493788686E-4 2.877706371331115E-6 68-69 0.27845694046229835 0.0 2.877706371331115E-6 1.4820187812355243E-4 2.877706371331115E-6 70-71 0.33089306710750827 0.0 2.877706371331115E-6 1.5107958449488352E-4 2.877706371331115E-6 72-73 0.3930975680302017 0.0 2.877706371331115E-6 1.5827385042321132E-4 2.877706371331115E-6 74-75 0.4719150678345896 0.0 2.877706371331115E-6 1.8129550139386023E-4 2.877706371331115E-6 76-77 0.5632649788861244 0.0 2.877706371331115E-6 2.50360454305807E-4 2.877706371331115E-6 78-79 0.6673847119604412 0.0 2.877706371331115E-6 2.6187127979113145E-4 2.877706371331115E-6 80-81 0.7888901080771549 0.0 2.877706371331115E-6 2.805763712047837E-4 2.877706371331115E-6 82-83 0.9292574306047583 0.0 2.877706371331115E-6 2.9640375624710486E-4 2.877706371331115E-6 84-85 1.121577425107188 0.0 2.877706371331115E-6 2.9928146261843595E-4 2.877706371331115E-6 86-87 1.3316399182420597 0.0 2.877706371331115E-6 2.9928146261843595E-4 2.877706371331115E-6 88-89 1.5738622189297424 0.0 2.877706371331115E-6 3.0359802217543264E-4 2.877706371331115E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCGAAA 40395 0.0 60.666748 1 CCGAAAC 54485 0.0 45.096928 2 CCAAACG 56820 0.0 43.135075 8 CCCGATC 2060 0.0 43.057423 1 TACGCAT 12490 0.0 39.633923 9 ACAATTT 49205 0.0 38.513634 26-27 CTACGCA 13190 0.0 37.772884 8 CGAAACC 64835 0.0 37.637005 3 CGGCAAT 17270 0.0 37.381428 1 ACTCGTC 49350 0.0 37.283276 42-43 TCGTACC 7430 0.0 36.354927 2 CTAACGA 49520 0.0 36.152363 94-95 CTCGTAC 7705 0.0 36.13751 1 TCGTCTA 51095 0.0 35.996464 44-45 TCCACTC 95210 0.0 35.878574 2 CTCCACT 97835 0.0 35.624207 1 CGTACCA 7730 0.0 35.00313 3 CAAACGA 70435 0.0 34.926785 9 CTCTACG 14190 0.0 34.710854 6 CTCCGAT 2340 0.0 34.192883 1 >>END_MODULE