##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2032138_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31711279
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16762095909156	34.0	31.0	34.0	30.0	34.0
2	32.418757250377695	34.0	31.0	34.0	31.0	34.0
3	32.48530234305592	34.0	31.0	34.0	31.0	34.0
4	35.82995044129251	37.0	35.0	37.0	35.0	37.0
5	35.94143162752912	37.0	35.0	37.0	35.0	37.0
6	35.86567154859947	37.0	35.0	37.0	35.0	37.0
7	35.99246129429217	37.0	35.0	37.0	35.0	37.0
8	35.92001943535611	37.0	35.0	37.0	35.0	37.0
9	37.71729024237717	39.0	38.0	39.0	35.0	39.0
10-11	37.69093607041205	39.0	38.0	39.0	35.0	39.0
12-13	37.61768254443474	39.0	38.0	39.0	35.0	39.0
14-15	39.05071958781606	41.0	39.0	41.0	36.0	41.0
16-17	38.880161440350605	41.0	39.0	41.0	36.0	41.0
18-19	38.77241372068279	40.5	38.5	41.0	35.0	41.0
20-21	38.693516981765384	41.0	39.0	41.0	35.0	41.0
22-23	38.57582343178274	41.0	39.0	41.0	34.5	41.0
24-25	38.53103105680474	41.0	39.0	41.0	34.5	41.0
26-27	38.39868823960081	41.0	39.0	41.0	34.0	41.0
28-29	38.30435670853894	41.0	39.0	41.0	34.0	41.0
30-31	38.16556408841157	40.0	39.0	41.0	34.0	41.0
32-33	38.07564445760765	40.0	38.5	41.0	34.0	41.0
34-35	38.06799189651102	40.0	38.0	41.0	34.0	41.0
36-37	37.96442656885583	40.0	38.0	41.0	34.0	41.0
38-39	37.85313586689455	40.0	38.0	41.0	33.0	41.0
40-41	37.84239317499619	40.0	38.0	41.0	33.0	41.0
42-43	37.75132247109932	40.0	38.0	41.0	33.0	41.0
44-45	37.63011359459831	40.0	38.0	41.0	33.0	41.0
46-47	37.53198089235063	40.0	38.0	41.0	33.0	41.0
48-49	37.41915706080477	40.0	38.0	41.0	32.0	41.0
50-51	37.314927805340176	40.0	38.0	41.0	32.0	41.0
52-53	37.23641305984536	40.0	37.5	41.0	32.0	41.0
54-55	37.07164977483248	40.0	37.0	41.0	31.0	41.0
56-57	36.96872207519603	40.0	37.0	41.0	31.0	41.0
58-59	36.81713422848697	40.0	37.0	41.0	31.0	41.0
60-61	36.701489429045104	40.0	36.0	41.0	31.0	41.0
62-63	36.59310980487416	39.0	36.0	41.0	31.0	41.0
64-65	36.32085151784638	39.0	35.5	41.0	30.5	41.0
66-67	36.03810786061325	39.0	35.0	41.0	30.0	41.0
68-69	35.73761561304418	38.0	35.0	40.5	30.0	41.0
70-71	35.36392964471726	37.0	35.0	40.0	29.0	41.0
72-73	34.988752850365955	37.0	35.0	39.5	29.0	41.0
74-75	34.56493632754453	36.5	34.0	39.0	28.5	41.0
76-77	34.34538154389799	36.0	34.0	39.0	29.0	40.0
78-79	34.20726743629609	36.0	34.0	38.0	29.5	39.5
80-81	33.890919458026275	35.0	34.0	37.0	29.0	39.0
82-83	33.56554885408438	35.0	34.0	37.0	29.0	39.0
84-85	33.29476605153643	35.0	34.0	36.0	29.0	37.5
86-87	33.032515670528454	35.0	34.0	36.0	29.0	37.0
88-89	32.813888096408846	35.0	34.0	36.0	29.0	37.0
90-91	32.62123743100996	35.0	34.0	35.0	29.0	36.0
92-93	32.44670837779832	35.0	34.0	35.0	29.0	36.0
94-95	32.27220592395532	35.0	34.0	35.0	28.5	36.0
96-97	32.16721331233597	35.0	34.0	35.0	28.5	36.0
98-99	32.01415956764153	35.0	34.0	35.0	27.0	35.5
100-101	31.677410188974086	35.0	33.5	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.05207894819223924
1101	2	0.062456407583191265
1101	3	0.01748499139063142
1101	4	0.16155756653650144
1101	5	0.05462858568125739
1101	6	0.0637623859751173
1101	7	0.0332847955187745
1101	8	0.03724771321135023
1101	9	-0.0018732270063139822
1101	10-11	0.02642940820326345
1101	12-13	0.026699982982428594
1101	14-15	0.05228548537203892
1101	16-17	0.04324484253258731
1101	18-19	-0.018242023360649284
1101	20-21	0.017201770177592834
1101	22-23	0.012306784819784866
1101	24-25	-0.01564844536370913
1101	26-27	-0.008442451543153595
1101	28-29	-0.03839629475649531
1101	30-31	4.021257711457338E-4
1101	32-33	-0.012045502454697044
1101	34-35	-0.02641691696503301
1101	36-37	-0.0548570446920138
1101	38-39	-0.04529818343983294
1101	40-41	-0.011018196047665185
1101	42-43	-0.03389193699641879
1101	44-45	-0.03470399140913116
1101	46-47	-0.06543210845464387
1101	48-49	-0.04685299407769605
1101	50-51	-0.04828306251694414
1101	52-53	-0.1457556721986748
1101	54-55	-0.1643549351673954
1101	56-57	-0.16225008293783816
1101	58-59	-0.12015986781180032
1101	60-61	-0.11307755204603609
1101	62-63	-0.15944529807978824
1101	64-65	-0.1402958297633532
1101	66-67	-0.21201806547369273
1101	68-69	-0.13797184256960549
1101	70-71	-0.15458772166952173
1101	72-73	-0.13001349144716556
1101	74-75	-0.22230155187379097
1101	76-77	-0.12124788497045813
1101	78-79	-0.09440618997528816
1101	80-81	-0.07501686093154092
1101	82-83	-0.07517305290814846
1101	84-85	-0.06760419687222452
1101	86-87	-0.07732189989160076
1101	88-89	-0.09067550927753132
1101	90-91	-0.11740683382668493
1101	92-93	-0.08848625074294603
1101	94-95	-0.06485913676880983
1101	96-97	-0.06849521081583276
1101	98-99	-0.11984457554009609
1101	100-101	-0.15241070700034598
1102	1	-0.009776022757101543
1102	2	-0.002531804823256323
1102	3	-0.019103415590038253
1102	4	0.12454710829195648
1102	5	0.029214645245680515
1102	6	0.05683190173043329
1102	7	-0.0012321316734755783
1102	8	-0.015147312506705646
1102	9	-0.023764817712944364
1102	10-11	-0.046177651118753715
1102	12-13	0.0013974844819273358
1102	14-15	0.036351066447963376
1102	16-17	0.025618597393680886
1102	18-19	-0.044314667320954015
1102	20-21	-0.01625436445677053
1102	22-23	-0.02605873206228182
1102	24-25	-0.05042627872023786
1102	26-27	-0.06159185561489977
1102	28-29	-0.09538574921213439
1102	30-31	-0.11832141872889679
1102	32-33	-0.11857598421865134
1102	34-35	-0.11865413713208284
1102	36-37	-0.1207137485518075
1102	38-39	-0.09450383089880177
1102	40-41	-0.08051382749579261
1102	42-43	-0.09864413498871727
1102	44-45	-0.10631176924514563
1102	46-47	-0.10438105714206358
1102	48-49	-0.12560655894453987
1102	50-51	-0.0322236021658
1102	52-53	-0.06136489697882297
1102	54-55	-0.09094036819988105
1102	56-57	-0.07174056350758917
1102	58-59	-0.10867166102038794
1102	60-61	-0.08029280237436609
1102	62-63	-0.11698852398474457
1102	64-65	-0.11832414961047988
1102	66-67	-0.13123857289783558
1102	68-69	-0.1673416894363342
1102	70-71	-0.16388803922789918
1102	72-73	-0.1579622773022038
1102	74-75	-0.14231173724044055
1102	76-77	-0.11633263312954512
1102	78-79	-0.08292865162987084
1102	80-81	-0.13346624294458564
1102	82-83	-0.11282137310870155
1102	84-85	-0.111891237987642
1102	86-87	-0.1168268166795059
1102	88-89	-0.07401863240446716
1102	90-91	-0.08854427206350124
1102	92-93	-0.11035047792243802
1102	94-95	-0.11178341560707139
1102	96-97	-0.13460911644109075
1102	98-99	-0.14846136620580097
1102	100-101	-0.11574032655427757
1103	1	-0.04775573106288533
1103	2	0.03580903796211743
1103	3	7.721312740684994E-4
1103	4	0.12530355751051303
1103	5	0.03143441154588089
1103	6	0.04509615788413868
1103	7	0.021152416113906725
1103	8	-0.015221849160269585
1103	9	-0.03706522113595412
1103	10-11	-0.0583188707043405
1103	12-13	-0.014458310982519151
1103	14-15	0.018081635017765052
1103	16-17	0.013531101231961884
1103	18-19	-0.058327322506364965
1103	20-21	-0.06291749647546396
1103	22-23	-0.05211613379889002
1103	24-25	-0.0963231625412817
1103	26-27	-0.0444111768280564
1103	28-29	-0.04065987181773778
1103	30-31	-0.03926431519681017
1103	32-33	-0.04179031887723994
1103	34-35	-0.05186336824845483
1103	36-37	-0.07198624874240522
1103	38-39	-0.04817486385493197
1103	40-41	-0.06498148495833078
1103	42-43	-0.03722630380467962
1103	44-45	-0.09050968882998234
1103	46-47	-0.2140416881300382
1103	48-49	-0.08048884841214488
1103	50-51	-0.08836879993638291
1103	52-53	-0.1532622495898437
1103	54-55	-0.11948979629929113
1103	56-57	-0.17751259503623373
1103	58-59	-0.1641911078496534
1103	60-61	-0.16786462150589188
1103	62-63	-0.14683533710672236
1103	64-65	-0.20367419062786496
1103	66-67	-0.16801034724783648
1103	68-69	-0.12419807420099716
1103	70-71	-0.11393445463235707
1103	72-73	-0.1268660025851034
1103	74-75	-0.15751512570745518
1103	76-77	-0.1333830560563598
1103	78-79	-0.11891758534200392
1103	80-81	-0.10780615890775636
1103	82-83	-0.09899748005076248
1103	84-85	-0.09168751612850201
1103	86-87	-0.10137417552013517
1103	88-89	-0.08269518058531844
1103	90-91	-0.07785386247983439
1103	92-93	-0.1352269936898267
1103	94-95	-0.13323742072746825
1103	96-97	-0.15945664825775907
1103	98-99	-0.19100456241484665
1103	100-101	-0.21967055366938482
1104	1	-0.06093274729054343
1104	2	-0.012413198065310382
1104	3	-0.056463640929948156
1104	4	0.07440784406549028
1104	5	-0.0011444060774223885
1104	6	0.005577888931647124
1104	7	-0.028097884172133547
1104	8	-0.03446010235941088
1104	9	-0.09612658874202396
1104	10-11	-0.06787156872904632
1104	12-13	-0.07447262447760039
1104	14-15	-0.05662204182154795
1104	16-17	-0.021298623615400913
1104	18-19	-0.05015852510836538
1104	20-21	-0.03871395787035681
1104	22-23	-0.08463787589388261
1104	24-25	-0.08392474348102041
1104	26-27	-0.08200416285395562
1104	28-29	-0.1018173388867396
1104	30-31	-0.1636935801348045
1104	32-33	-0.18493377662984756
1104	34-35	-0.1891590492125701
1104	36-37	-0.16025355643866845
1104	38-39	-0.1624867446262641
1104	40-41	-0.10635403935064147
1104	42-43	-0.14169767815860013
1104	44-45	-0.11126982928131213
1104	46-47	-0.16841660421513893
1104	48-49	-0.20450836790100624
1104	50-51	-0.08462284219081084
1104	52-53	-0.1707390676827245
1104	54-55	-0.16971953386393324
1104	56-57	-0.16632076258969875
1104	58-59	-0.1575798944381006
1104	60-61	-0.15441727494290802
1104	62-63	-0.1574313380053738
1104	64-65	-0.1622403413564868
1104	66-67	-0.17721454757852229
1104	68-69	-0.15838625446782117
1104	70-71	-0.16931908815021757
1104	72-73	-0.13479487724757178
1104	74-75	-0.1278659645890059
1104	76-77	-0.11915514648411119
1104	78-79	-0.1277137746657857
1104	80-81	-0.10485324638811733
1104	82-83	-0.10531939046710903
1104	84-85	-0.12216694897675495
1104	86-87	-0.13801341736344597
1104	88-89	-0.14006249961228434
1104	90-91	-0.12828909557870105
1104	92-93	-0.1000779971330985
1104	94-95	-0.1369909724603886
1104	96-97	-0.15946356000302586
1104	98-99	-0.14678383443219545
1104	100-101	-0.18393970519730374
1105	1	-0.09404168453704642
1105	2	-0.09614085632684777
1105	3	-0.06463898645539246
1105	4	0.08316497661547828
1105	5	-0.010684658155000193
1105	6	-0.011752937896069682
1105	7	-0.019840649631156282
1105	8	-0.043890337750241315
1105	9	-0.07085578003137272
1105	10-11	-0.07696993111801476
1105	12-13	-0.06694273723675082
1105	14-15	-0.10047101435270633
1105	16-17	-0.0306299765989948
1105	18-19	-0.0809322291116743
1105	20-21	-0.07998152116053348
1105	22-23	-0.0735801743425668
1105	24-25	-0.03635594850668156
1105	26-27	-0.04885514329871654
1105	28-29	-0.1009302568139887
1105	30-31	-0.09929015810013198
1105	32-33	-0.09598512671696113
1105	34-35	-0.14271798083677822
1105	36-37	-0.1218426654292557
1105	38-39	-0.12715004538134878
1105	40-41	-0.11539847440312911
1105	42-43	-0.10597604146028772
1105	44-45	-0.13750052180892425
1105	46-47	-0.10292902075401855
1105	48-49	-0.11112212663348942
1105	50-51	-0.11026846397540169
1105	52-53	-0.11156757792137029
1105	54-55	-0.12222108550436417
1105	56-57	-0.12655365515289674
1105	58-59	-0.10240012390240594
1105	60-61	-0.09292308389026971
1105	62-63	-0.11521504141113326
1105	64-65	-0.10802141423715028
1105	66-67	-0.11454225221689995
1105	68-69	-0.11772640018951108
1105	70-71	-0.17467526602351313
1105	72-73	-0.1593940802478997
1105	74-75	-0.1289542929677765
1105	76-77	-0.10869925093368948
1105	78-79	-0.08536804990320945
1105	80-81	-0.09168658887937653
1105	82-83	-0.06565930042594914
1105	84-85	-0.06961399431554582
1105	86-87	-0.11971591750163668
1105	88-89	-0.08299534226323146
1105	90-91	-0.14538853297323584
1105	92-93	-0.1836520227778493
1105	94-95	-0.18262896497942194
1105	96-97	-0.1651163711510648
1105	98-99	-0.14486857328002856
1105	100-101	-0.20651398977978985
1106	1	-0.08206745378278413
1106	2	-0.0703704139966419
1106	3	-0.11485400902913057
1106	4	0.07149503609423391
1106	5	0.0013171413189994041
1106	6	-0.020121522991871643
1106	7	-0.013291341122346978
1106	8	-0.054366318648249035
1106	9	-0.06661112767054078
1106	10-11	-0.05688376606460821
1106	12-13	-0.048054393072540336
1106	14-15	-0.03904813421440423
1106	16-17	-0.05042961616459962
1106	18-19	-0.08266851994602575
1106	20-21	-0.08945464633185907
1106	22-23	-0.09372729525532009
1106	24-25	-0.0767154367367695
1106	26-27	-0.09921249105692453
1106	28-29	-0.052749796182830266
1106	30-31	-0.07092656349504978
1106	32-33	-0.08530508038525397
1106	34-35	-0.06337681195284972
1106	36-37	-0.10804982965160548
1106	38-39	-0.08784164161809827
1106	40-41	-0.11157511964682243
1106	42-43	-0.09919387523972034
1106	44-45	-0.12715084970803758
1106	46-47	-0.13009904388522386
1106	48-49	-0.10251339147229999
1106	50-51	-0.0853654934759902
1106	52-53	-0.1397977226348459
1106	54-55	-0.11619660642310237
1106	56-57	-0.1048583412236539
1106	58-59	-0.1471299819638645
1106	60-61	-0.09660006744873328
1106	62-63	-0.11320399902836442
1106	64-65	-0.16103317377162796
1106	66-67	-0.15916941296526232
1106	68-69	-0.1653202136039198
1106	70-71	-0.12700367866649032
1106	72-73	-0.11260928858224162
1106	74-75	-0.12576017600086686
1106	76-77	-0.14594262561285376
1106	78-79	-0.09708195873636782
1106	80-81	-0.09243283404028801
1106	82-83	-0.0937884269600957
1106	84-85	-0.09249232102170879
1106	86-87	-0.09321849049059949
1106	88-89	-0.06895925864203889
1106	90-91	-0.09631508176865822
1106	92-93	-0.08018848056201477
1106	94-95	-0.10796323560517607
1106	96-97	-0.14114840979994625
1106	98-99	-0.13431627326913542
1106	100-101	-0.12110704717091281
1107	1	-0.11926906232584855
1107	2	-0.08688187523541302
1107	3	-0.13879719247819367
1107	4	0.04459585374593189
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2216	34-35	-0.16979559080894546
2216	36-37	-0.16600017623422048
2216	38-39	-0.16973202683684718
2216	40-41	-0.2288654826933012
2216	42-43	-0.2721428626697744
2216	44-45	-0.2141898894792149
2216	46-47	-0.18090984503044183
2216	48-49	-0.20864966328738888
2216	50-51	-0.17572963249027396
2216	52-53	-0.19098714024952557
2216	54-55	-0.21986502637805927
2216	56-57	-0.22151477056827673
2216	58-59	-0.3307048830382726
2216	60-61	-0.3763914440111549
2216	62-63	-0.3444471837759977
2216	64-65	-0.2816812885713702
2216	66-67	-0.2357881322257711
2216	68-69	-0.26418181122998163
2216	70-71	-0.30465423006116765
2216	72-73	-0.30582260017387597
2216	74-75	-0.2862788019319993
2216	76-77	-0.24119108354166485
2216	78-79	-0.2822816289467909
2216	80-81	-0.23717479996685853
2216	82-83	-0.25146484442754513
2216	84-85	-0.22655677340937075
2216	86-87	-0.22479389784821535
2216	88-89	-0.22919796007474247
2216	90-91	-0.2288652853981361
2216	92-93	-0.2133428095401726
2216	94-95	-0.2510148157274372
2216	96-97	-0.22403298657658155
2216	98-99	-0.25777801612410656
2216	100-101	-0.2174191062737485
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	58215.0
3	16719.0
4	18818.0
5	36352.0
6	104234.0
7	96356.0
8	81002.0
9	95528.0
10	122071.0
11	118711.0
12	96256.0
13	69544.0
14	55060.0
15	49779.0
16	47199.0
17	45061.0
18	45260.0
19	45487.0
20	48271.0
21	53502.0
22	64162.0
23	78989.0
24	98784.0
25	119464.0
26	143484.0
27	173522.0
28	213711.0
29	265264.0
30	333990.0
31	436526.0
32	584301.0
33	780324.0
34	999118.0
35	1461572.0
36	2724093.0
37	6688628.0
38	1.1059879E7
39	4176957.0
40	5086.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.254521544567922	17.715119172036516	16.59796480938842	39.43239447400714
2	17.57040493965904	21.901415611529824	50.48196003325033	10.046219415560815
3	14.1328965585822	23.054899521906247	44.00766128295929	18.80454263655227
4	18.417404461296886	30.83244603293545	36.21747616856146	14.532673337206203
5	20.421029865719873	33.77630108195857	33.600183692398865	12.202485359922688
6	16.9525660332517	35.63908125521773	33.43942144696538	13.968931264565185
7	14.410974821700648	16.6316486287086	53.66157766719951	15.295798882391235
8	15.425029056696198	21.810671843289576	43.56003427045626	19.204264829557964
9	17.68910599307954	23.016638902313364	41.12968883163449	18.164566272972603
10-11	19.665250288026705	30.780251562583	33.101604329508575	16.452893819881727
12-13	20.076675014161044	22.96765841942631	38.71380103741236	18.241865529000282
14-15	20.18453229175559	26.428109404389037	37.39727125107494	15.990087052780424
16-17	21.237121295895538	27.262431810787625	34.80888256424473	16.691564329072108
18-19	21.380351466939075	27.837076458294884	32.14444591335071	18.638126161415332
20-21	22.874956995638644	27.10821968400855	31.886116772275656	18.130706548077146
22-23	22.558208487486095	29.045325315673516	30.649662526847347	17.74680366999304
24-25	21.99176601632163	29.093503612125055	29.663399435096704	19.25133093645661
26-27	22.891682997746486	28.759214878774486	29.764153256015952	18.584948867463076
28-29	23.249167770053646	28.66507189340417	29.52821097359332	18.557549362948865
30-31	22.26074506438613	30.386676006934994	28.168609337137525	19.183969591541352
32-33	22.385538877493044	28.944441300080086	29.282427264110684	19.38759255831619
34-35	23.31482495952108	29.36594763472123	27.96756917592498	19.35165822983271
36-37	23.331318001101202	29.61392515195499	28.131706592565013	18.92305025437879
38-39	23.968562537075922	27.39318566561183	29.656851940823753	18.981399856488498
40-41	23.570122266587	29.528946877410316	27.315620635880627	19.58531022012206
42-43	23.742797201703084	29.160182234519127	27.930646837405643	19.166373726372147
44-45	24.345351658041032	28.076772963162544	28.271211137146075	19.306664241650353
46-47	22.599459518411646	28.756073005153493	28.055625016905328	20.58884245952953
48-49	22.996382408773567	28.403421715197464	28.55748271841454	20.04271315761443
50-51	22.575528189304432	28.695763446505822	29.201757112755168	19.52695125143458
52-53	24.82538215256335	27.176949608144383	27.415785200401793	20.58188303889047
54-55	23.528195194547504	27.28237983821402	28.706820381234007	20.48260458600447
56-57	24.012194790165466	27.570218814627594	28.43271001689559	19.98487637831135
58-59	23.442479178035637	27.82900654857402	27.879392934899272	20.849121338491074
60-61	23.72761967140967	27.600093610050987	27.46475712172625	21.207529596813092
62-63	24.44771775156703	27.293920808102335	27.923751933625297	20.33460950670534
64-65	24.48211207369426	27.331847681651944	27.69331889103695	20.49272135361684
66-67	24.392811664917208	27.09098913400931	27.129719897697147	21.386479303376337
68-69	25.16190876092479	26.541756138786642	27.74454727297907	20.5517878273095
70-71	23.92192611538853	26.447180504903546	27.99562559698704	21.635267782720884
72-73	24.332961615708353	25.999367254021738	28.068592036407452	21.599079093862457
74-75	25.227216127799046	26.34201152160511	27.999689507361563	20.43108284323428
76-77	23.511377599064765	26.622552267349864	28.27823032031475	21.58783981327062
78-79	23.179930490284878	26.99723539256577	28.058339283873696	21.764494833275656
80-81	23.12620992785285	26.7683385581767	28.889069108786856	21.216382405183595
82-83	23.067476013705527	27.567070138035593	27.538644484749618	21.826809363509266
84-85	22.830977529112488	27.54770212283078	27.68787332981813	21.9334470182386
86-87	23.29516924201705	28.326728787462923	27.8371753005954	20.540926669924627
88-89	24.081929153006303	26.12564503292802	29.132758781720575	20.6596670323451
90-91	24.39678864779969	26.2698734341893	27.84588214818942	21.48745576982159
92-93	23.807758355578184	26.444024262899518	28.357202680113247	21.39101470140905
94-95	24.604382107325414	26.824450213385177	27.252593557455295	21.318574121834118
96-97	24.353125191436654	26.58214612493709	27.76904312018998	21.29568556343628
98-99	26.193162607841113	25.61343295152755	27.59619831784671	20.597206122784627
100-101	26.64139839297597	26.23460524653015	26.290611017813802	20.83338534268008
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	42.0
1	83.5
2	161.0
3	274.0
4	432.0
5	653.5
6	902.0
7	1110.5
8	1372.0
9	1651.5
10	1975.5
11	2463.0
12	3070.0
13	3863.5
14	4772.0
15	5850.0
16	7302.0
17	9085.0
18	11416.5
19	14286.5
20	20234.5
21	29043.5
22	39564.0
23	55079.5
24	69865.5
25	91463.5
26	121851.0
27	190458.0
28	278680.0
29	397908.5
30	550925.5
31	624935.0
32	796448.5
33	1056410.5
34	1388383.5
35	1468577.5
36	1293829.0
37	1433652.5
38	1530153.5
39	1595721.5
40	1660997.5
41	1539909.0
42	1301947.5
43	1097875.0
44	1006897.0
45	895476.5
46	803364.0
47	777297.5
48	793905.0
49	779838.5
50	713443.5
51	666676.0
52	697251.0
53	723028.0
54	790984.0
55	703351.5
56	538122.0
57	557542.0
58	545307.0
59	420839.5
60	293555.5
61	207661.0
62	148212.5
63	108805.0
64	82543.5
65	64651.0
66	48553.0
67	30697.5
68	16564.5
69	11483.0
70	8441.0
71	13677.0
72	40027.5
73	91607.5
74	105488.5
75	70002.5
76	42434.5
77	82860.0
78	88865.0
79	27428.5
80	6323.5
81	1676.5
82	549.0
83	291.5
84	205.0
85	170.0
86	131.5
87	87.0
88	47.0
89	33.0
90	33.5
91	28.0
92	19.0
93	14.5
94	10.0
95	10.0
96	11.0
97	10.0
98	12.0
99	11.5
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014095930977744545
2	0.016155135212300963
3	0.013616606255458822
4	0.005676213816541427
5	0.005509080854165485
6	0.006631709808992566
7	9.460356360902378E-6
8	0.0
9	0.0030083933227669562
10-11	7.678655912932431E-4
12-13	5.25049778030082E-4
14-15	0.004528357244751938
16-17	0.005706171611684284
18-19	0.006694778851398583
20-21	0.0026252488901504097
22-23	0.008225779855804619
24-25	0.012314230529774595
26-27	0.014532684096406201
28-29	0.019707499025819804
30-31	0.015256401358015235
32-33	0.01742124623860173
34-35	0.016618692673985178
36-37	0.018927019626045358
38-39	0.027099190795804865
40-41	0.022305943572947656
42-43	0.030623173540240998
44-45	0.026487421084466507
46-47	0.02837003200028608
48-49	0.038306559631353876
50-51	0.04646927044475248
52-53	0.039374003174075696
54-55	0.03704044860505311
56-57	0.029063791466752253
58-59	0.038599830678541856
60-61	0.030131235009474074
62-63	0.03605184136533881
64-65	0.041564075671624595
66-67	0.05716104985863232
68-69	0.048621501516857776
70-71	0.04607508892971488
72-73	0.0407883264500306
74-75	0.041830542375790015
76-77	0.040318462084105784
78-79	0.04817844149395551
80-81	0.04866564984654199
82-83	0.057001800526557136
84-85	0.05530366655977515
86-87	0.04906613826582019
88-89	0.04280968925914341
90-91	0.036220551053774905
92-93	0.0579115714632639
94-95	0.09076423565255756
96-97	0.08361693642189581
98-99	0.07598400556470775
100-101	0.20915586533107036
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.1711279E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.34630540530101
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.86510396070061	9.2430743769842
2	8.810510215489197	2.1755449979390806
3	3.878678580082473	1.4366205095609244
4	2.1662530837433374	1.069808886282814
5	1.4644219680638233	0.9040100429973962
6	1.0402695529068033	0.7706091362414
7	0.847006467761093	0.7320180371870589
8	0.6443575636560445	0.636434821689057
9	0.5291422549940784	0.5879656694705901
>10	4.324686092940837	11.047090784252454
>50	0.6697026114791821	5.8179322362747
>100	0.603933739675839	15.203079481689905
>500	0.08063332028848212	6.907137842256944
>1k	0.06191798296785984	15.207261261307575
>5k	0.007075860247482833	6.084950333597639
>10k+	0.006306745003191221	22.17646158226838
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA	206026	0.6496931265370911	No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT	171223	0.5399435323942626	No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA	164898	0.51999794773336	No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT	150554	0.47476483051976553	No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC	125547	0.3959064533474036	No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA	110885	0.3496705383595534	No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG	97510	0.3074931162505303	No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC	93088	0.2935485509745602	No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA	88663	0.27959452534222917	No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT	82888	0.2613833393474921	No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG	81469	0.25690859078878525	No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG	81176	0.2559846293175371	No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA	78597	0.24785187629928143	No Hit
CAAAGACCAAGAGGTACAGGTGCAAGGGAGAGAAGAAGGGCATGGCCAGA	76340	0.24073453486376253	No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG	74936	0.2363070880868602	No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA	72286	0.2279504399680631	No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA	70521	0.2223845969757322	No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA	65675	0.2071029680007546	No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA	64955	0.204832482474138	No Hit
CGCAGGATATTTTATTGAGACAAGGTGGGTCAACGGGAAGCTGAGCAGTG	62695	0.1977056806822582	No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT	62041	0.1956433229955815	No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT	61974	0.1954320417035213	No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT	61292	0.1932813873574762	No Hit
GTGAAATAAATGCTTTTTATTTGTCAGAAGACAGATTTTCAAATGTCTTT	60127	0.18960761563732575	No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT	59681	0.1882011759916716	No Hit
CCAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTC	59038	0.18617350627831822	No Hit
CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT	57134	0.18016933344126548	No Hit
CTCAGGCTTTATTCAAAGACCAAGAGGTACAGGTGCAAGGGAGAGAAGAA	55581	0.17527202229843836	No Hit
CGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGA	54851	0.17297000225061876	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	54315	0.17127975191413755	No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGC	53467	0.1686056245161225	No Hit
GTTTATTTTACACTGGGCGAATTGCTTCTGTACAATAGAAAGCAGTCTGT	51956	0.163840758362348	No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA	51929	0.16375561515509987	No Hit
CGAACGTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGA	51094	0.1611224826346487	No Hit
CTCAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCT	48986	0.15447500556505464	No Hit
CAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCA	46946	0.14804196323964103	No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGG	46714	0.14731036234773123	No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC	46671	0.14717476390655831	No Hit
CAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTG	46277	0.14593230377115976	No Hit
CTTTATTCAAAGACCAAGAGGTACAGGTGCAAGGGAGAGAAGAAGGGCAT	46120	0.14543721178827254	No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT	46091	0.14534576167678384	No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC	44537	0.14044529708183642	No Hit
CGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAG	43765	0.1380108320449642	No Hit
GATTTATTTACCTGAAATCATGTCTGTGCAATACCTGTTACCAGCACAGA	43528	0.13726346389245292	No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC	43192	0.13620390398003185	No Hit
CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT	42880	0.135220026918498	No Hit
CAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGT	42591	0.13430867925573106	No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA	42197	0.13306621912033256	No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG	42048	0.13259635475440773	No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA	41668	0.13139804294869342	No Hit
GTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGTGGGTCACCTGA	40279	0.12701789795359564	No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG	39762	0.12538756320740013	No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT	39394	0.12422709282712942	No Hit
GTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGAGGTTATTCCTC	39217	0.12366893180183619	No Hit
AGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATAATAAAT	39029	0.12307608280321965	No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA	39021	0.12305085518625723	No Hit
CTCGCAGGATATTTTATTGAGACAAGGTGGGTCAACGGGAAGCTGAGCAG	38543	0.12154350507275345	No Hit
AATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAA	38432	0.12119347188740007	No Hit
AACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCC	37999	0.11982802711930983	No Hit
GCCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCT	35255	0.11117495450120443	No Hit
GTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCT	34610	0.10914097788361043	No Hit
TGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACA	33732	0.10637224692198632	No Hit
CTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGTGGGTC	33690	0.1062398019329337	No Hit
TATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAAT	32953	0.103915707720272	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8920712721804757E-5	0.0	0.0	3.1534521203007928E-6	0.0
2	1.8920712721804757E-5	0.0	0.0	3.1534521203007928E-6	0.0
3	1.8920712721804757E-5	0.0	0.0	3.1534521203007928E-6	0.0
4	1.8920712721804757E-5	0.0	0.0	3.1534521203007928E-6	0.0
5	2.5227616962406342E-5	0.0	0.0	3.1534521203007928E-6	0.0
6	2.5227616962406342E-5	0.0	0.0	3.1534521203007928E-6	0.0
7	2.5227616962406342E-5	0.0	0.0	3.1534521203007928E-6	0.0
8	2.5227616962406342E-5	0.0	0.0	3.1534521203007928E-6	0.0
9	2.5227616962406342E-5	0.0	0.0	3.1534521203007928E-6	0.0
10-11	2.680434302255674E-5	0.0	0.0	3.1534521203007928E-6	0.0
12-13	3.9418151503759903E-5	0.0	0.0	3.1534521203007928E-6	0.0
14-15	5.676213816541427E-5	0.0	0.0	3.1534521203007928E-6	0.0
16-17	7.252939876691823E-5	0.0	0.0	3.1534521203007928E-6	0.0
18-19	9.145011148872298E-5	0.0	0.0	3.1534521203007928E-6	0.0
20-21	1.3559844117293408E-4	0.0	0.0	3.1534521203007928E-6	0.0
22-23	3.42149555052636E-4	3.1534521203007928E-6	0.0	3.1534521203007928E-6	0.0
24-25	7.252939876691823E-4	3.1534521203007928E-6	0.0	3.1534521203007928E-6	0.0
26-27	0.0012913386432631744	3.1534521203007928E-6	0.0	3.1534521203007928E-6	0.0
28-29	0.0023635123641654442	3.1534521203007928E-6	0.0	4.730178180451189E-6	0.0
30-31	0.004422716598721862	3.1534521203007928E-6	0.0	6.3069042406015855E-6	0.0
32-33	0.0083566481187971	3.1534521203007928E-6	0.0	6.3069042406015855E-6	0.0
34-35	0.014480652136421239	3.1534521203007928E-6	0.0	6.3069042406015855E-6	0.0
36-37	0.02351056228290256	3.1534521203007928E-6	0.0	6.3069042406015855E-6	0.0
38-39	0.0387827309015193	3.1534521203007928E-6	0.0	6.3069042406015855E-6	0.0
40-41	0.057583612442752626	3.1534521203007928E-6	0.0	6.3069042406015855E-6	0.0
42-43	0.07881580556873787	3.1534521203007928E-6	0.0	6.3069042406015855E-6	0.0
44-45	0.10410649157355022	3.1534521203007928E-6	0.0	6.3069042406015855E-6	0.0
46-47	0.13310406054577617	3.1534521203007928E-6	0.0	6.3069042406015855E-6	0.0
48-49	0.1657659408817916	7.883630300751983E-6	0.0	6.3069042406015855E-6	0.0
50-51	0.20108775808128082	9.460356360902378E-6	0.0	6.3069042406015855E-6	0.0
52-53	0.24322260858667982	9.460356360902378E-6	0.0	6.3069042406015855E-6	0.0
54-55	0.2958978728041843	1.5767260601503965E-5	0.0	6.3069042406015855E-6	0.0
56-57	0.3527987628628918	1.8920712721804757E-5	0.0	9.460356360902378E-6	0.0
58-59	0.41435730170328355	1.8920712721804757E-5	0.0	9.460356360902378E-6	0.0
60-61	0.47983085135102876	2.680434302255674E-5	0.0	9.460356360902378E-6	0.0
62-63	0.5575886737334057	2.8381069082707133E-5	0.0	9.460356360902378E-6	0.0
64-65	0.6469606602748506	3.1534521203007924E-5	0.0	1.1037082421052774E-5	0.0
66-67	0.7478553608638743	3.468797332330872E-5	0.0	1.2613808481203171E-5	0.0
68-69	0.8652221186032893	4.4148329684211095E-5	0.0	1.2613808481203171E-5	0.0
70-71	0.9936354191201181	5.0455233924812684E-5	0.0	1.2613808481203171E-5	0.0
72-73	1.1359980150910975	5.360868604511348E-5	0.0	1.2613808481203171E-5	0.0
74-75	1.311262153759235	5.8338864225564663E-5	0.0	1.734398666165436E-5	0.0
76-77	1.5039680361047563	6.622249452631664E-5	0.0	1.8920712721804757E-5	0.0
78-79	1.719041354339571	7.252939876691823E-5	0.0	2.2074164842105548E-5	0.0
80-81	1.9593958982228372	7.883630300751982E-5	0.0	2.2074164842105548E-5	0.0
82-83	2.231956648610736	8.198975512782061E-5	0.0	2.2074164842105548E-5	0.0
84-85	2.589629071725552	8.198975512782061E-5	0.0	2.2074164842105548E-5	0.0
86-87	2.966977459344986	8.198975512782061E-5	0.0	2.2074164842105548E-5	0.0
88-89	3.3906358680770965	8.51432072481214E-5	0.0	2.2074164842105548E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	48555	0.0	83.686356	1
GCGTGTT	53655	0.0	74.85506	2
GTAAACG	16070	0.0	58.774097	1
TAAACGC	16955	0.0	55.621807	2
CGTGTTT	71585	0.0	55.554016	3
CGAACGT	9905	0.0	51.920757	1
GTGTTTT	85600	0.0	45.401215	4
TCGAACG	5235	0.0	38.18819	2
GTAGATA	33610	0.0	37.3698	2
TAGATAT	33745	0.0	37.233433	3
AAACGCT	25450	0.0	37.185375	3
GAACGTT	14185	0.0	36.488903	2
GTTTTTT	257625	0.0	35.961376	1
ACGTTTA	14750	0.0	35.731686	4
CGCAGGA	17620	0.0	35.601143	1
AACGTTT	15040	0.0	34.44527	3
CCAAGAC	25575	0.0	34.108337	1
AGATATA	38510	0.0	33.499527	4
ATTGAAG	55305	0.0	33.120827	6
AACGCTT	28725	0.0	32.893917	4
>>END_MODULE