FastQCFastQC Report
Fri 27 May 2016
SRR2032138_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2032138_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31711279
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC1383920.43641254583266725No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT1128570.3558891459407866No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT663500.2092315481819576No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA622520.19630870139296497No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC571170.1801157247552204No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA505140.15929348040487423No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC436900.13777432313594162No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC427160.13470286077076865No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA424320.13380728036860323No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG396480.1250280696656858No Hit
CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT391850.12356802133398656No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT389650.12287426186752039No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT385120.12144574805702413No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC384370.12120923914800157No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG323550.10202994335233215No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT317700.10018517386195618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA335050.066.552611
CCGAAAC484850.045.955532
CCAAACG498500.044.7525568
CGAAACC552850.040.2038353
CAAACGA581550.038.5028849
ACAATTT460200.038.1568626-27
ACTCGTC448250.037.89518742-43
CTAACGA441800.036.90266494-95
TACGCAT64800.036.7886549
TCGTCTA462900.036.6268144-45
CGGCAAT225950.035.836721
TCCACTC737300.035.7686122
CGAGCTA481200.035.76525512-13
TAGGTAG476300.034.2947676-77
TCGTACC41400.034.2240872
TCGTGTT60150.033.8898622
TCCGCTC25000.033.822232
CGCCTAT7750.033.6275441
CTCCACT803700.033.570181
CTACCTA516550.033.4370216-17
Produced by FastQC (version 0.11.5)