##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2032138_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31711279
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75559919863213	34.0	31.0	34.0	31.0	34.0
2	32.90264271586145	34.0	31.0	34.0	31.0	34.0
3	32.94319283053831	34.0	31.0	34.0	31.0	34.0
4	36.350258467972864	37.0	37.0	37.0	35.0	37.0
5	36.320138206976765	37.0	37.0	37.0	35.0	37.0
6	36.2342080242175	37.0	37.0	37.0	35.0	37.0
7	36.20902395642888	37.0	37.0	37.0	35.0	37.0
8	36.20680228634108	37.0	37.0	37.0	35.0	37.0
9	38.02172655981489	39.0	39.0	39.0	35.0	39.0
10-11	38.064946166315146	39.0	39.0	39.0	36.0	39.0
12-13	37.976462901417506	39.0	38.5	39.0	35.0	39.0
14-15	39.45724857076878	41.0	39.0	41.0	36.0	41.0
16-17	39.42844911742601	41.0	39.0	41.0	36.0	41.0
18-19	39.4016391927932	41.0	39.0	41.0	36.0	41.0
20-21	39.42022768933413	41.0	39.0	41.0	36.0	41.0
22-23	39.40008741053933	41.0	39.0	41.0	36.0	41.0
24-25	39.415968116580856	41.0	39.0	41.0	36.0	41.0
26-27	39.38352289417276	41.0	39.0	41.0	36.0	41.0
28-29	29.59427448511301	21.5	20.5	41.0	19.0	41.0
30-31	30.584234461183357	26.5	23.0	40.5	20.5	41.0
32-33	37.5709802338783	38.5	37.0	40.0	33.5	41.0
34-35	38.8322752450319	40.0	38.5	41.0	35.0	41.0
36-37	39.073753726552624	41.0	39.0	41.0	35.0	41.0
38-39	39.06140693662971	41.0	39.0	41.0	35.0	41.0
40-41	38.98731296836056	41.0	39.0	41.0	35.0	41.0
42-43	38.894809761536266	41.0	38.5	41.0	35.0	41.0
44-45	38.799047398876596	40.5	38.0	41.0	35.0	41.0
46-47	38.68973345414419	40.0	38.0	41.0	34.5	41.0
48-49	38.58417951228016	40.0	38.0	41.0	34.0	41.0
50-51	38.53116380137175	40.0	38.0	41.0	34.0	41.0
52-53	38.439891150401095	40.0	38.0	41.0	34.0	41.0
54-55	38.31585042659427	40.0	37.5	41.0	34.0	41.0
56-57	38.17304212170061	40.0	37.0	41.0	34.0	41.0
58-59	37.99692877414374	40.0	37.0	41.0	34.0	41.0
60-61	37.76480415690581	39.0	36.0	41.0	33.0	41.0
62-63	37.456044204334994	39.0	36.0	41.0	33.0	41.0
64-65	37.07619416738126	38.5	35.0	40.5	33.0	41.0
66-67	36.705716978492106	37.5	35.0	40.0	32.0	41.0
68-69	36.25213762270516	37.0	35.0	39.0	32.0	41.0
70-71	35.88954781672477	36.5	35.0	39.0	32.0	41.0
72-73	35.56882250003224	36.0	35.0	39.0	32.0	40.5
74-75	35.21319269399383	35.5	35.0	37.5	31.0	39.5
76-77	34.90213589303667	35.0	34.5	37.0	31.0	39.0
78-79	34.5732953092179	35.0	34.0	37.0	31.0	39.0
80-81	34.21405863194606	35.0	34.0	36.0	31.0	38.0
82-83	33.989910782217265	35.0	34.0	36.0	31.0	37.0
84-85	33.79004691043839	35.0	34.0	35.5	31.0	37.0
86-87	33.54452482033285	35.0	34.0	35.0	30.0	36.5
88-89	33.424816908204804	35.0	34.0	35.0	30.0	36.0
90-91	33.4034011526309	35.0	34.0	35.0	30.5	36.0
92-93	33.424631642892734	35.0	34.0	35.0	31.0	36.0
94-95	33.33508422980984	35.0	34.0	35.0	31.0	36.0
96-97	33.26754332425381	35.0	34.0	35.0	31.0	36.0
98-99	33.143004512684584	35.0	34.0	35.0	31.0	35.0
100-101	32.84946516979022	35.0	34.0	35.0	30.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.09187857562734791
1101	2	0.09237943108168167
1101	3	0.09261891993188698
1101	4	0.0852160103772377
1101	5	0.0675500467429373
1101	6	0.08467537155716087
1101	7	0.08612511490650121
1101	8	0.04263008781829569
1101	9	0.11074057721071284
1101	10-11	0.08722456207969032
1101	12-13	0.08406811446290163
1101	14-15	0.15295794774165472
1101	16-17	0.0654080328711899
1101	18-19	0.11126192629972564
1101	20-21	0.10545705328308941
1101	22-23	0.12656406640451934
1101	24-25	0.11834544840397143
1101	26-27	0.09070544035692762
1101	28-29	9.396990196458674
1101	30-31	8.239384892113435
1101	32-33	1.4627728028459046
1101	34-35	0.32713827736022694
1101	36-37	0.13327322898933147
1101	38-39	0.1268995757801079
1101	40-41	0.09316815251249722
1101	42-43	0.11436253773312899
1101	44-45	0.08930968926745209
1101	46-47	0.10017041391242287
1101	48-49	0.05568154512500456
1101	50-51	0.10044095524669672
1101	52-53	0.07801449695472229
1101	54-55	0.06698989731962257
1101	56-57	0.07574671286182877
1101	58-59	0.14571561351004192
1101	60-61	0.12703846020091447
1101	62-63	0.15083832984453238
1101	64-65	0.2226366484789395
1101	66-67	0.23565057287617464
1101	68-69	0.24615144343388806
1101	70-71	0.28765370863321493
1101	72-73	0.2587716399923323
1101	74-75	0.24658476231756055
1101	76-77	0.26119697472597636
1101	78-79	0.2905871342856088
1101	80-81	0.28274577535472645
1101	82-83	0.23461072444219866
1101	84-85	0.20103209070313
1101	86-87	0.21742238458635654
1101	88-89	0.19370859443785804
1101	90-91	0.1703249083682863
1101	92-93	0.1345017923584777
1101	94-95	0.13328211271122115
1101	96-97	0.1031824192329367
1101	98-99	0.12456115178110139
1101	100-101	0.06873566090124683
1102	1	0.011964644668736923
1102	2	0.03265678846445752
1102	3	0.02483051739001496
1102	4	0.03983888100457733
1102	5	0.01788030869513335
1102	6	0.04682712827423785
1102	7	0.05429059930920488
1102	8	0.038110761901378964
1102	9	0.049130361304854375
1102	10-11	0.0431738557536292
1102	12-13	0.0803643706629984
1102	14-15	0.06996913321855658
1102	16-17	0.05266228079901225
1102	18-19	0.12918923436057383
1102	20-21	0.11160421312892765
1102	22-23	0.11707030416666697
1102	24-25	0.0913106470397338
1102	26-27	0.06596726850733603
1102	28-29	9.397399761558603
1102	30-31	8.270886960117245
1102	32-33	1.4672978703511248
1102	34-35	0.3292883649805489
1102	36-37	0.11959183298137077
1102	38-39	0.08485485114469782
1102	40-41	0.06757310184291754
1102	42-43	0.07198531614128001
1102	44-45	0.07728704533170827
1102	46-47	0.09337249846032591
1102	48-49	0.09155533850976383
1102	50-51	0.023876482955735412
1102	52-53	0.04873090966068361
1102	54-55	0.07286727655954195
1102	56-57	0.08862120955001274
1102	58-59	0.13441174248247734
1102	60-61	0.10806822654936354
1102	62-63	0.17475764903986146
1102	64-65	0.20440120939962725
1102	66-67	0.20963221146163846
1102	68-69	0.11979760368609504
1102	70-71	0.18353534583870612
1102	72-73	0.1982635382707798
1102	74-75	0.2059493227271929
1102	76-77	0.20027124166013266
1102	78-79	0.1902058662532582
1102	80-81	0.18535218373917672
1102	82-83	0.19173710058286275
1102	84-85	0.16487729123945627
1102	86-87	0.14833697544288782
1102	88-89	0.11131933307797226
1102	90-91	0.08616695494137616
1102	92-93	0.0685114114595251
1102	94-95	0.07486699076140724
1102	96-97	0.06638524449121519
1102	98-99	0.07489973937510541
1102	100-101	-0.006991305590752006
1103	1	-0.002257577259982213
1103	2	0.017762830763139448
1103	3	0.03884384278127584
1103	4	0.02909799063530727
1103	5	0.008045034168326026
1103	6	0.052464081619099545
1103	7	0.02693858669023541
1103	8	0.00825320060123147
1103	9	0.038825000418654554
1103	10-11	0.017178837955327708
1103	12-13	0.06859647631679167
1103	14-15	0.06407853890022608
1103	16-17	0.04836647947934836
1103	18-19	0.10375163043364921
1103	20-21	0.07869591568779555
1103	22-23	0.06877605667177988
1103	24-25	0.07214722919056982
1103	26-27	0.042914921001028006
1103	28-29	9.375733013814013
1103	30-31	8.21624215131132
1103	32-33	1.3848209247925212
1103	34-35	0.29743822520170937
1103	36-37	0.05936742523404348
1103	38-39	0.06359020035435492
1103	40-41	0.042126023631432474
1103	42-43	0.0734775135681005
1103	44-45	0.08884859116695054
1103	46-47	0.08157007882194023
1103	48-49	0.06765510235300098
1103	50-51	0.0425544822602788
1103	52-53	0.029923424718411695
1103	54-55	0.019596589073593407
1103	56-57	0.06269244522559347
1103	58-59	0.09453689348426764
1103	60-61	0.044731588821207424
1103	62-63	0.1269626910298598
1103	64-65	0.15578249254273402
1103	66-67	0.1688051011547813
1103	68-69	0.1917729443792382
1103	70-71	0.22877789076581934
1103	72-73	0.19135955991757214
1103	74-75	0.23619148576139537
1103	76-77	0.20976282165531757
1103	78-79	0.16261481649667076
1103	80-81	0.14217180576255117
1103	82-83	0.1627584631779797
1103	84-85	0.10089966154806262
1103	86-87	0.12189894965954551
1103	88-89	0.14883203232444941
1103	90-91	0.12693085414046834
1103	92-93	0.0833484205657129
1103	94-95	0.07198106209273902
1103	96-97	0.05584376297549909
1103	98-99	0.019772184473396237
1103	100-101	-0.06170596391618943
1104	1	0.0028979446679358034
1104	2	0.006949599063169387
1104	3	0.014167145380810098
1104	4	0.023095299880985465
1104	5	-0.0024496847286741286
1104	6	0.04018474263864391
1104	7	0.04225371020599056
1104	8	0.03659232118311451
1104	9	0.026250111356247885
1104	10-11	0.013908834087608568
1104	12-13	0.029214097326253352
1104	14-15	0.014150129374932874
1104	16-17	0.04843608294157775
1104	18-19	0.09502301894612941
1104	20-21	0.054627100903033465
1104	22-23	0.03660858952485313
1104	24-25	0.057705971970122505
1104	26-27	0.03269078423591054
1104	28-29	9.385438249210736
1104	30-31	8.22868178482657
1104	32-33	1.324378818786954
1104	34-35	0.22776744114501213
1104	36-37	0.029490368534240474
1104	38-39	0.0498399186147509
1104	40-41	-0.033059586902389526
1104	42-43	0.007012087073768214
1104	44-45	0.041922584845707433
1104	46-47	0.030101153318987883
1104	48-49	0.01587399412822066
1104	50-51	0.0317718642394027
1104	52-53	-0.005844776155818465
1104	54-55	0.06165286748713328
1104	56-57	0.08308613516944519
1104	58-59	0.09195959742960724
1104	60-61	0.07813102058051413
1104	62-63	0.12173177291211346
1104	64-65	0.17734356901782178
1104	66-67	0.16508734954744142
1104	68-69	0.2065583322410518
1104	70-71	0.2158867543661529
1104	72-73	0.13650766321545404
1104	74-75	0.12507825547978513
1104	76-77	0.17592365252241393
1104	78-79	0.18423369716274607
1104	80-81	0.20721353089331984
1104	82-83	0.202067591172856
1104	84-85	0.15255592439579857
1104	86-87	0.13626118566370593
1104	88-89	0.10076915397980457
1104	90-91	0.05193426404967738
1104	92-93	0.050334495671499724
1104	94-95	0.04599826498289872
1104	96-97	0.020324323036774672
1104	98-99	0.02219222021216183
1104	100-101	-0.07757838707255615
1105	1	-0.012669543630636326
1105	2	-0.020298654760658508
1105	3	-0.019338713451091394
1105	4	-7.85284126749275E-4
1105	5	-0.015180695089227925
1105	6	0.003193153771249513
1105	7	-0.0073304835578440475
1105	8	-0.008962236032395765
1105	9	-0.011208404810041372
1105	10-11	-0.023268767988504635
1105	12-13	-0.010618851615383562
1105	14-15	-0.005603244637576665
1105	16-17	-0.03068625184518936
1105	18-19	0.0593023441336058
1105	20-21	0.012497483090243122
1105	22-23	0.03003441274674401
1105	24-25	0.015250008054692898
1105	26-27	-0.018010687536403225
1105	28-29	9.320870799470818
1105	30-31	8.166992750970458
1105	32-33	1.380469410334129
1105	34-35	0.20811517991671025
1105	36-37	0.0023967279614041104
1105	38-39	0.02285732497762183
1105	40-41	-0.0434773888492046
1105	42-43	-0.01586217806367074
1105	44-45	-0.05031011323868029
1105	46-47	-0.007394220197873835
1105	48-49	-0.04834129681135124
1105	50-51	-0.05098991413797904
1105	52-53	-0.08394080441953378
1105	54-55	-0.0443231538375386
1105	56-57	-0.021482987241149942
1105	58-59	-0.0013032442035694203
1105	60-61	-0.017488914504951936
1105	62-63	0.05711551118409375
1105	64-65	0.06878991123022615
1105	66-67	0.10064875043283195
1105	68-69	0.1151341445423455
1105	70-71	0.16455893741645866
1105	72-73	0.08951262042130281
1105	74-75	0.13127914032160248
1105	76-77	0.10899813372606104
1105	78-79	0.09623905640712138
1105	80-81	0.06757694968823102
1105	82-83	0.03909609802599334
1105	84-85	0.05767347242187526
1105	86-87	0.032107033858146394
1105	88-89	0.03989894603132882
1105	90-91	-0.0020077567988110445
1105	92-93	-0.003726735909644674
1105	94-95	-0.03238464394370055
1105	96-97	-0.041417041795824616
1105	98-99	-0.04620317163691823
1105	100-101	-0.13019819382457598
1106	1	-0.03608373763585604
1106	2	-0.03572934156603225
1106	3	-0.03054764848417335
1106	4	7.687722720106649E-5
1106	5	-0.012804591136770682
1106	6	-0.041237704081460436
1106	7	-0.031449562676996834
1106	8	-0.024694672007164797
1106	9	-0.01466268479730104
1106	10-11	-0.022744649006931184
1106	12-13	0.008669045921799068
1106	14-15	-0.007785185123402982
1106	16-17	-0.07715058720461343
1106	18-19	-0.020351636064610545
1106	20-21	-0.06082386742674828
1106	22-23	-0.08845423164001431
1106	24-25	-0.07601836999462819
1106	26-27	-0.11194186763670189
1106	28-29	9.254196246786499
1106	30-31	8.143686894693381
1106	32-33	1.3638787432707105
1106	34-35	0.16599070207167443
1106	36-37	-0.0726942654679874
1106	38-39	-0.10443574703533898
1106	40-41	-0.10240592578934837
1106	42-43	-0.12537252255744846
1106	44-45	-0.12581698209324088
1106	46-47	-0.09005549232448118
1106	48-49	-0.07108390923391283
1106	50-51	-0.07052795158627845
1106	52-53	-0.05572397478051272
1106	54-55	-0.022296977737532586
1106	56-57	0.006769329185118522
1106	58-59	0.039519481542185986
1106	60-61	0.034606707537676584
1106	62-63	0.014054430463360745
1106	64-65	0.07592309574452116
1106	66-67	0.09267547513827168
1106	68-69	0.09212499733634871
1106	70-71	0.16100980041888135
1106	72-73	0.13532560111840297
1106	74-75	0.1138828688871314
1106	76-77	0.13052297973052873
1106	78-79	0.14282414645397523
1106	80-81	0.17219356615983372
1106	82-83	0.12958889675553564
1106	84-85	0.12690572318308568
1106	86-87	0.10063553024051686
1106	88-89	0.07662197713902685
1106	90-91	0.07999602669163153
1106	92-93	0.048145490096089816
1106	94-95	0.018310061362825536
1106	96-97	1.6543590007245257E-4
1106	98-99	0.012982744526127021
1106	100-101	-0.10424403367365898
1107	1	-0.09684194964764714
1107	2	-0.07465303682140956
1107	3	-0.059615392363618014
1107	4	-0.02560760670991158
1107	5	-0.025862212690149988
1107	6	-0.005970941452964951
1107	7	-0.02586403822752459
1107	8	-0.0649777051200644
1107	9	-0.05001869462540043
1107	10-11	-0.06632634610807742
1107	12-13	-0.03490757391119104
1107	14-15	-0.05404180056365959
1107	16-17	-0.07808246950636999
1107	18-19	-0.018957369668321178
1107	20-21	-0.04726808917028791
1107	22-23	-0.0702302196837934
1107	24-25	-0.0815664062248942
1107	26-27	-0.05878279808295872
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2216	40-41	-0.34804075765585196
2216	42-43	-0.3489104508241354
2216	44-45	-0.3028761903217614
2216	46-47	-0.29537182500686043
2216	48-49	-0.4010220223456571
2216	50-51	-0.3802740151722688
2216	52-53	-0.38213217592777937
2216	54-55	-0.41666988553765094
2216	56-57	-0.4261550201316453
2216	58-59	-0.45643419915605676
2216	60-61	-0.4452489060599589
2216	62-63	-0.4209679394674879
2216	64-65	-0.4883903247976562
2216	66-67	-0.4752565599879901
2216	68-69	-0.46268002380389106
2216	70-71	-0.5020729228699139
2216	72-73	-0.5590290656009387
2216	74-75	-0.5641848923689636
2216	76-77	-0.5384039635224127
2216	78-79	-0.538598540491698
2216	80-81	-0.5466506417752086
2216	82-83	-0.507715277546879
2216	84-85	-0.4700308212231121
2216	86-87	-0.4610112411906684
2216	88-89	-0.46664272621874403
2216	90-91	-0.4815394902904373
2216	92-93	-0.49171002870000535
2216	94-95	-0.45262530880772545
2216	96-97	-0.5109457149301591
2216	98-99	-0.506501045656421
2216	100-101	-0.6054546749029939
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2095.0
3	965.0
4	1149.0
5	1704.0
6	2813.0
7	5191.0
8	7759.0
9	8677.0
10	10823.0
11	15112.0
12	17107.0
13	17985.0
14	19698.0
15	22416.0
16	24930.0
17	26831.0
18	28862.0
19	31482.0
20	35782.0
21	41455.0
22	49640.0
23	61942.0
24	77397.0
25	99669.0
26	128831.0
27	167412.0
28	214911.0
29	274458.0
30	354324.0
31	463003.0
32	626768.0
33	903444.0
34	1443099.0
35	2441857.0
36	4852774.0
37	9841321.0
38	7814309.0
39	1569199.0
40	4085.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.77872806529133	10.387090758048213	7.098079265617035	55.73610191104342
2	19.464972378896327	14.968906943464248	42.74692648604944	22.81919419158999
3	22.645582980112533	19.998461746055717	22.360141954539266	34.99581331929249
4	23.385010316444223	30.059560046359906	20.116645243516743	26.438784393679132
5	22.376021277162188	34.79157384909683	23.98132835395855	18.851076519782428
6	18.874365497287833	36.14960811073102	25.271295578124064	19.704730813857076
7	16.14347281290665	23.807027161944426	40.99801347131028	19.051486553838647
8	18.07019515043843	22.07523386237433	32.45038460921113	27.404186377976114
9	17.74939446624023	22.478396409050546	34.8542737743249	24.917935350384322
10-11	22.52436901078635	33.54041317601854	23.50071405193086	20.43450376126425
12-13	22.328465527990847	27.24165272551763	26.97597438438229	23.453907362109234
14-15	21.8515910380026	29.559329663114504	26.219019737425285	22.370059561457612
16-17	22.61533191392249	26.801296787808525	27.324916475302054	23.258454822966932
18-19	22.31005252106041	27.672258189270764	25.995712755704364	24.021976533964462
20-21	22.72724162276772	26.747356989290783	26.104317646727527	24.42108374121397
22-23	22.220688418149265	28.630046741413363	26.3560593062172	22.793205534220174
24-25	21.59622133184852	29.324719132268363	26.324162138020355	22.754897397862763
26-27	21.41320148354973	30.29272523851875	26.3737187941474	21.920354483784124
28-29	21.57089762168613	28.802836501577016	26.006367743585372	23.61989813315148
30-31	21.293113890103616	27.733358885253867	27.328437714255603	23.645089510386914
32-33	21.798008880711713	27.081660349992713	28.60591927759871	22.514411491696865
34-35	21.40141682711694	29.087325679926064	26.073562028198232	23.43769546475877
36-37	21.61027222777266	28.72551893474843	26.110443171614612	23.553765665864304
38-39	20.977861648861364	28.337062022270025	26.516398366042253	24.16867796282636
40-41	21.222926042878107	28.492821186457768	25.860634491217745	24.423618279446384
42-43	20.814771846097617	28.528803797514257	27.005947727732128	23.650476628655994
44-45	20.59711608553242	27.732854293391068	26.710958372946042	24.95907124813047
46-47	20.886232624045217	29.152192190040648	26.384489253807768	23.577085932106364
48-49	20.63831294852535	28.17679318453223	26.79027547264808	24.394618394294344
50-51	20.42017289810354	28.574525486657286	27.201015764769377	23.804285850469796
52-53	22.54176029922981	27.805138354715996	26.4468692669255	23.206232079128693
54-55	21.754392183298567	28.072439146967238	26.325633223434476	23.847535446299723
56-57	22.563214657044558	28.06660860740487	25.64520343977318	23.7249732957774
58-59	20.773715875666827	29.908098314167653	26.26181838960201	23.056367420563518
60-61	20.705856739490073	30.16378336553376	25.568975000976778	23.56138489399939
62-63	21.461229340383074	29.226836824946528	26.529379872688757	22.782553961981648
64-65	22.646727683232204	28.434742414520713	25.72571260843815	23.192817293808933
66-67	22.85289248181685	28.27023703239649	25.529537826734373	23.34733265905229
68-69	20.838627000891233	29.110660381342107	25.48509452379729	24.56561809396937
70-71	20.694198257945104	30.212135615906195	25.5310856275126	23.562580498636102
72-73	20.254855752832164	29.67686928950146	26.527996706151143	23.540278251515232
74-75	20.562823242071413	28.21463130841741	27.6106887112755	23.611856738235677
76-77	20.009404826583115	28.720055801361333	27.023713150171595	24.246826221883957
78-79	21.850958565887904	30.013841236837884	25.51218773445274	22.623012462821464
80-81	20.35272178232206	29.674926477073765	25.64805328345431	24.324298457149865
82-83	20.953342333555028	29.75263581299848	25.029835703381202	24.264186150065296
84-85	20.722113721351874	29.292875997933542	25.937179925104388	24.0478303556102
86-87	21.100893317088424	29.405826676425466	26.43288900127942	23.060391005206686
88-89	20.394770671407976	29.37698504611536	26.739732610353073	23.48851167212359
90-91	20.711813163839786	31.034762468624947	25.944528361100993	22.30889600643427
92-93	21.494203739796287	29.649960834087207	26.587601664571398	22.268233761545105
94-95	20.10179814402988	28.707212867055294	27.52283061439998	23.66815837451485
96-97	20.221778578576355	29.88737382249892	27.9344248531388	21.956422745785925
98-99	21.215860693142425	29.068442877579997	26.004535499064147	23.71116093021343
100-101	21.478257368943495	30.627180523313292	24.99620065261383	22.898361455129386
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	113.0
1	91.0
2	58.0
3	49.5
4	47.5
5	68.5
6	81.0
7	81.0
8	96.0
9	112.5
10	124.5
11	132.5
12	173.5
13	210.5
14	279.0
15	366.0
16	476.0
17	716.5
18	1201.0
19	2083.0
20	5008.5
21	8523.5
22	12952.0
23	26906.0
24	44904.5
25	52883.5
26	64860.5
27	104205.5
28	166009.0
29	231210.5
30	290460.0
31	355105.5
32	459926.5
33	600255.5
34	719471.5
35	884252.5
36	1072183.5
37	1303205.0
38	1552982.0
39	1571999.0
40	1589653.0
41	1636466.0
42	1374792.5
43	1117415.5
44	1085406.0
45	1069323.5
46	1037129.0
47	1010806.5
48	1042640.0
49	1065804.5
50	999122.5
51	924393.5
52	897359.5
53	864803.5
54	847084.0
55	834042.5
56	764788.5
57	657402.5
58	564768.5
59	546481.5
60	515800.0
61	386337.0
62	254236.0
63	189091.5
64	156950.5
65	123196.5
66	81993.0
67	54532.0
68	30794.5
69	19450.0
70	29785.5
71	55293.5
72	110819.0
73	143179.0
74	72483.0
75	8022.0
76	5455.5
77	5259.5
78	2370.0
79	1484.5
80	575.0
81	176.5
82	121.5
83	101.5
84	76.5
85	70.5
86	56.5
87	27.5
88	10.5
89	6.0
90	9.0
91	9.0
92	4.5
93	1.5
94	1.0
95	1.5
96	0.5
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.03668410851545912
2	5.991559028571506E-5
3	0.0
4	8.072837427970028E-4
5	0.029349178883639474
6	0.049165472007609665
7	5.802351901353459E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.2613808481203172E-4
28-29	26.0137063535028
30-31	0.18315565259919034
32-33	0.05565212301906839
34-35	0.0
36-37	0.001805351338872204
38-39	5.597377513533907E-4
40-41	4.872083525864725E-4
42-43	2.8381069082707136E-5
44-45	2.3650890902255945E-4
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	6.306904240601585E-6
58-59	0.0
60-61	0.0
62-63	0.001297645547503776
64-65	0.0
66-67	0.0014695086880601694
68-69	0.0038535184910075686
70-71	0.001065866816661668
72-73	0.003982810027939901
74-75	0.004397488981759456
76-77	0.009750473955970052
78-79	0.00987030513654148
80-81	0.0013307567947669343
82-83	0.002992626062165452
84-85	0.009081942106466283
86-87	0.0013323335208270848
88-89	0.006813033305909862
90-91	0.011234173178571574
92-93	0.014185804363173115
94-95	0.015778297683925017
96-97	0.016243431871669385
98-99	3.3111247263158323E-4
100-101	0.04744684060204572
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.1711279E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.14723738180055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.00185894378916	15.01493165633428
2	10.696563622057212	4.5240554017035235
3	4.645460955168286	2.94715996700489
4	2.744023888512519	2.3211409820682265
5	1.821213957320762	1.9256821939255264
6	1.2949201855704184	1.6430390732845717
7	0.9814080829907708	1.4527848789596551
8	0.7435251508383642	1.257880229129436
9	0.6233348010772733	1.186362810604667
>10	4.549619168444062	19.070040388929378
>50	0.5111587619999742	7.503540234205547
>100	0.3252011598694068	13.172321390706943
>500	0.03214703553061026	4.688509235454491
>1k	0.025100197949708837	10.57475378371574
>5k	0.0025631132334490185	3.724813940992902
>10k+	0.0019009756481413553	8.992983832980372
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC	138392	0.43641254583266725	No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT	112857	0.3558891459407866	No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT	66350	0.2092315481819576	No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA	62252	0.19630870139296497	No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC	57117	0.1801157247552204	No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA	50514	0.15929348040487423	No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC	43690	0.13777432313594162	No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC	42716	0.13470286077076865	No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA	42432	0.13380728036860323	No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG	39648	0.1250280696656858	No Hit
CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT	39185	0.12356802133398656	No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	38965	0.12287426186752039	No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT	38512	0.12144574805702413	No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC	38437	0.12120923914800157	No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG	32355	0.10202994335233215	No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT	31770	0.10018517386195618	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5767260601503962E-5	0.0	0.0	0.0	0.0
2	1.5767260601503962E-5	0.0	0.0	9.460356360902378E-6	0.0
3	1.5767260601503962E-5	0.0	0.0	1.2613808481203171E-5	0.0
4	1.5767260601503962E-5	0.0	0.0	1.2613808481203171E-5	0.0
5	2.2074164842105548E-5	0.0	0.0	1.2613808481203171E-5	0.0
6	2.2074164842105548E-5	0.0	0.0	1.2613808481203171E-5	0.0
7	2.2074164842105548E-5	0.0	0.0	1.5767260601503962E-5	0.0
8	2.2074164842105548E-5	0.0	0.0	1.5767260601503962E-5	0.0
9	2.2074164842105548E-5	0.0	0.0	1.8920712721804757E-5	0.0
10-11	2.6804343022556736E-5	0.0	0.0	2.2074164842105548E-5	0.0
12-13	4.25716036240607E-5	0.0	0.0	2.2074164842105548E-5	0.0
14-15	6.306904240601585E-5	0.0	0.0	2.2074164842105548E-5	0.0
16-17	8.19897551278206E-5	1.5767260601503964E-6	0.0	2.2074164842105548E-5	0.0
18-19	9.302683754887338E-5	3.1534521203007928E-6	0.0	2.3650890902255945E-5	0.0
20-21	1.0879409815037734E-4	3.1534521203007928E-6	0.0	3.1534521203007924E-5	0.0
22-23	2.0812783993985233E-4	3.1534521203007928E-6	0.0	3.1534521203007924E-5	0.0
24-25	3.8156770655639594E-4	3.1534521203007928E-6	0.0	3.311124726315832E-5	0.0
26-27	6.149231634586546E-4	3.1534521203007928E-6	0.0	4.730178180451189E-5	0.0
28-29	0.0011068616942255783	3.1534521203007928E-6	0.0	5.991559028571506E-5	0.0
30-31	0.0029815889797443996	3.1534521203007928E-6	0.0	6.149231634586545E-5	0.0
32-33	0.006920250678000089	3.1534521203007928E-6	0.0	6.306904240601585E-5	3.1534521203007928E-6
34-35	0.013075789216827238	3.1534521203007928E-6	0.0	6.622249452631664E-5	3.1534521203007928E-6
36-37	0.02211200626754916	3.1534521203007928E-6	0.0	6.937594664661744E-5	3.1534521203007928E-6
38-39	0.037352640364962886	3.1534521203007928E-6	0.0	7.095267270676783E-5	3.1534521203007928E-6
40-41	0.056137754645594715	3.1534521203007928E-6	0.0	7.410612482706863E-5	3.1534521203007928E-6
42-43	0.07730845545523407	3.1534521203007928E-6	0.0	7.725957694736942E-5	3.1534521203007928E-6
44-45	0.10257076039096374	3.1534521203007928E-6	0.0	7.883630300751982E-5	3.1534521203007928E-6
46-47	0.1314942232383626	3.1534521203007928E-6	0.0	8.829665936842219E-5	3.1534521203007928E-6
48-49	0.16394955246049836	1.1037082421052774E-5	0.0	9.302683754887338E-5	3.1534521203007928E-6
50-51	0.1989544477218973	1.2613808481203171E-5	0.0	9.618028966917418E-5	3.1534521203007928E-6
52-53	0.24063047094379258	1.2613808481203171E-5	0.0	1.0091046784962537E-4	3.1534521203007928E-6
54-55	0.2926892352717782	1.2613808481203171E-5	0.0	1.0406391996992616E-4	3.1534521203007928E-6
56-57	0.3488191062870722	1.2613808481203171E-5	0.0	1.0406391996992616E-4	3.1534521203007928E-6
58-59	0.40916198933508796	1.2613808481203171E-5	0.0	1.0721737209022696E-4	3.1534521203007928E-6
60-61	0.47323225278929937	1.4190534541353567E-5	0.0	1.1510100239097893E-4	3.1534521203007928E-6
62-63	0.5493849680424432	1.5767260601503962E-5	0.0	1.2140790663158052E-4	3.1534521203007928E-6
64-65	0.6370667042474067	1.5767260601503962E-5	0.0	1.245613587518813E-4	3.1534521203007928E-6
66-67	0.7359368885751976	1.5767260601503962E-5	0.0	1.292915369323325E-4	3.1534521203007928E-6
68-69	0.8504686928584622	1.5767260601503962E-5	0.0	1.308682629924829E-4	3.1534521203007928E-6
70-71	0.9756465514998622	1.5767260601503962E-5	0.0	1.371751672330845E-4	3.1534521203007928E-6
72-73	1.1147011761966459	1.5767260601503962E-5	0.0	1.4505879753383646E-4	3.1534521203007928E-6
74-75	1.2861196800040768	1.5767260601503962E-5	0.0	1.5609587995488926E-4	3.1534521203007928E-6
76-77	1.4741521463073122	1.5767260601503962E-5	0.0	1.8763040115789715E-4	3.1534521203007928E-6
78-79	1.6834341497231948	1.5767260601503962E-5	0.0	2.0024420963910033E-4	3.1534521203007928E-6
80-81	1.9170387293429572	1.5767260601503962E-5	0.0	2.112812920601531E-4	3.1534521203007928E-6
82-83	2.182012273929412	1.5767260601503962E-5	0.0	2.2074164842105548E-4	3.1534521203007928E-6
84-85	2.530973285561897	1.5767260601503962E-5	0.0	2.2389510054135627E-4	3.1534521203007928E-6
86-87	2.8986516122544286	1.5767260601503962E-5	0.0	2.2704855266165706E-4	3.1534521203007928E-6
88-89	3.3122993872306443	1.5767260601503962E-5	0.0	2.2704855266165706E-4	3.1534521203007928E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGAAA	33505	0.0	66.55261	1
CCGAAAC	48485	0.0	45.95553	2
CCAAACG	49850	0.0	44.752556	8
CGAAACC	55285	0.0	40.203835	3
CAAACGA	58155	0.0	38.502884	9
ACAATTT	46020	0.0	38.15686	26-27
ACTCGTC	44825	0.0	37.895187	42-43
CTAACGA	44180	0.0	36.902664	94-95
TACGCAT	6480	0.0	36.788654	9
TCGTCTA	46290	0.0	36.62681	44-45
CGGCAAT	22595	0.0	35.83672	1
TCCACTC	73730	0.0	35.768612	2
CGAGCTA	48120	0.0	35.765255	12-13
TAGGTAG	47630	0.0	34.29476	76-77
TCGTACC	4140	0.0	34.224087	2
TCGTGTT	6015	0.0	33.889862	2
TCCGCTC	2500	0.0	33.82223	2
CGCCTAT	775	0.0	33.627544	1
CTCCACT	80370	0.0	33.57018	1
CTACCTA	51655	0.0	33.43702	16-17
>>END_MODULE