##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2032137_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21458618
Sequences flagged as poor quality	0
Sequence length	101
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26952336818709	34.0	31.0	34.0	30.0	34.0
2	32.49847175619604	34.0	31.0	34.0	31.0	34.0
3	32.67585270402782	34.0	31.0	34.0	31.0	34.0
4	35.95069165218375	37.0	37.0	37.0	35.0	37.0
5	36.040079841115585	37.0	37.0	37.0	35.0	37.0
6	35.973454301670316	37.0	36.0	37.0	35.0	37.0
7	36.080642611746946	37.0	36.0	37.0	35.0	37.0
8	36.08411147446681	37.0	37.0	37.0	35.0	37.0
9	37.90221630302567	39.0	39.0	39.0	35.0	39.0
10-11	37.840859509219094	39.0	38.5	39.0	35.0	39.0
12-13	37.78649487119814	39.0	38.5	39.0	35.0	39.0
14-15	39.140789262384	41.0	39.0	41.0	36.0	41.0
16-17	38.939658229621315	41.0	39.0	41.0	36.0	41.0
18-19	38.77025598759435	41.0	39.0	41.0	35.0	41.0
20-21	38.525154345913606	41.0	39.0	41.0	34.0	41.0
22-23	38.304722512885036	41.0	39.0	41.0	34.0	41.0
24-25	38.17390418618757	41.0	39.0	41.0	34.0	41.0
26-27	37.95051717682844	41.0	39.0	41.0	33.5	41.0
28-29	37.74642430374593	41.0	39.0	41.0	33.0	41.0
30-31	37.6039068312787	41.0	39.0	41.0	33.0	41.0
32-33	37.48553443656064	41.0	38.0	41.0	32.0	41.0
34-35	37.34812735843474	40.5	38.0	41.0	32.0	41.0
36-37	37.25402451360101	40.0	38.0	41.0	31.0	41.0
38-39	37.17976821713309	40.0	38.0	41.0	31.0	41.0
40-41	37.13070555149451	40.0	38.0	41.0	31.0	41.0
42-43	37.01620258117275	40.0	38.0	41.0	31.0	41.0
44-45	36.927210037477714	40.0	38.0	41.0	31.0	41.0
46-47	36.853634912555876	40.0	38.0	41.0	30.0	41.0
48-49	36.769354461689936	40.0	38.0	41.0	30.0	41.0
50-51	36.658239943504284	40.0	38.0	41.0	30.0	41.0
52-53	36.57804948109892	40.0	37.5	41.0	30.0	41.0
54-55	36.4507500669428	40.0	37.0	41.0	29.5	41.0
56-57	36.34081607678556	40.0	37.0	41.0	29.0	41.0
58-59	36.19880919171962	40.0	37.0	41.0	29.0	41.0
60-61	36.13316705670421	40.0	36.0	41.0	29.0	41.0
62-63	36.064645891920904	40.0	36.0	41.0	29.0	41.0
64-65	35.838694388427065	39.0	35.5	41.0	28.5	41.0
66-67	35.62233707221965	39.0	35.0	41.0	28.0	41.0
68-69	35.36479301695943	39.0	35.0	41.0	28.0	41.0
70-71	35.02972430470592	38.0	35.0	40.0	28.0	41.0
72-73	34.7188033497777	37.0	35.0	40.0	27.5	41.0
74-75	34.3218987122097	37.0	34.5	39.5	26.0	41.0
76-77	34.06492498724755	36.0	34.0	39.0	27.0	41.0
78-79	33.904635168024335	36.0	35.0	39.0	28.0	40.0
80-81	33.56726786878819	36.0	34.0	38.0	28.0	39.0
82-83	33.209309984454734	35.0	34.0	37.0	28.0	39.0
84-85	32.90017008551063	35.0	34.0	37.0	27.0	39.0
86-87	32.60962525638883	35.0	34.0	36.0	27.0	37.0
88-89	32.37515955128144	35.0	34.0	36.0	26.5	37.0
90-91	32.16929927640261	35.0	34.0	36.0	26.0	36.5
92-93	31.966410022304323	35.0	34.0	35.0	26.0	36.0
94-95	31.773603220859798	35.0	34.0	35.0	25.5	36.0
96-97	31.665371320743954	35.0	34.0	35.0	25.0	36.0
98-99	31.52821446842476	35.0	34.0	35.0	25.0	36.0
100-101	31.182188503472126	35.0	33.5	35.0	22.5	35.5
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.13179929847405703
1101	2	0.11367738874489675
1101	3	0.10513861352002607
1101	4	0.2061581025720045
1101	5	0.11299242063233095
1101	6	0.10903562093467656
1101	7	0.08098280118942114
1101	8	0.08685664385376413
1101	9	0.09992849631795053
1101	10-11	0.12287139772980282
1101	12-13	0.11017836517326884
1101	14-15	0.14392267034708084
1101	16-17	0.16691034513540615
1101	18-19	0.11659480409214495
1101	20-21	0.09085222684338845
1101	22-23	0.04044360653423951
1101	24-25	0.07923528091988885
1101	26-27	0.04364819776947826
1101	28-29	5.298963231012976E-4
1101	30-31	0.024165180937039565
1101	32-33	0.03825459303708101
1101	34-35	0.0173890161028325
1101	36-37	-0.01666668278074468
1101	38-39	0.02728165453722653
1101	40-41	0.02984995859650752
1101	42-43	0.002695416333054368
1101	44-45	-0.014913375633859971
1101	46-47	-0.019794838797579928
1101	48-49	0.010103867437706526
1101	50-51	0.01601404835806619
1101	52-53	-0.04336510018386974
1101	54-55	-0.06767808363393613
1101	56-57	-0.06112253748584351
1101	58-59	0.01609431494065916
1101	60-61	-0.008532794831289436
1101	62-63	-0.05113709217372531
1101	64-65	-0.05469793301928405
1101	66-67	-0.1145694213819084
1101	68-69	-0.07332415540538761
1101	70-71	-0.07650358066049279
1101	72-73	-0.03475747489187597
1101	74-75	-0.10901993906294649
1101	76-77	-0.032249586254863516
1101	78-79	-0.03538528494304671
1101	80-81	-0.017197236332570753
1101	82-83	0.002875079425137983
1101	84-85	-0.004575371076107615
1101	86-87	-0.03717987110654519
1101	88-89	-0.0368779532821506
1101	90-91	-0.07144289103411694
1101	92-93	-0.0510630519622417
1101	94-95	-0.02156600921081875
1101	96-97	-0.00921632852621812
1101	98-99	-0.05148737992928787
1101	100-101	-0.04084487955472227
1102	1	0.010096127755829798
1102	2	0.027381803804246374
1102	3	0.029767664599049226
1102	4	0.14706786211186085
1102	5	0.06025421869840386
1102	6	0.07759000403095939
1102	7	0.030941809888837213
1102	8	0.02122732930994431
1102	9	0.01857996826854702
1102	10-11	0.0073062806894625965
1102	12-13	0.03878899955871873
1102	14-15	0.06511743411287085
1102	16-17	0.058889256653984035
1102	18-19	-0.0064634395676606005
1102	20-21	-0.020750231326978508
1102	22-23	0.005833944642716915
1102	24-25	-0.033346662827383966
1102	26-27	-0.05291329001021694
1102	28-29	-0.08648239939920188
1102	30-31	-0.08969208459905786
1102	32-33	-0.08693130097894652
1102	34-35	-0.11442717030083571
1102	36-37	-0.10428905031064772
1102	38-39	-0.07985182900605281
1102	40-41	-0.0921544166774737
1102	42-43	-0.06352144346418953
1102	44-45	-0.09569536412401902
1102	46-47	-0.08781697324546656
1102	48-49	-0.0840532590109504
1102	50-51	-0.018621600313068143
1102	52-53	-0.03469000153743451
1102	54-55	-0.05928587724141465
1102	56-57	-0.06481400176404861
1102	58-59	-0.11439802046075442
1102	60-61	-0.08737422640654557
1102	62-63	-0.1035035993044815
1102	64-65	-0.10526751358138142
1102	66-67	-0.10772178893105888
1102	68-69	-0.10043037403195854
1102	70-71	-0.11867549018388956
1102	72-73	-0.09441583570411183
1102	74-75	-0.08713614006953208
1102	76-77	-0.0772119911181477
1102	78-79	-0.05558679916966014
1102	80-81	-0.09342782357916235
1102	82-83	-0.09520765919865681
1102	84-85	-0.07613816083284775
1102	86-87	-0.10782946972233276
1102	88-89	-0.07333035758581019
1102	90-91	-0.09114388045082222
1102	92-93	-0.08024963700295018
1102	94-95	-0.06627334910164961
1102	96-97	-0.09964340060299648
1102	98-99	-0.08209524936359358
1102	100-101	-0.05585894880570663
1103	1	-0.019009040558763957
1103	2	0.056661779365065
1103	3	0.030447446127674027
1103	4	0.16587797295003526
1103	5	0.06997122990253501
1103	6	0.06948267342000491
1103	7	0.05369713536775578
1103	8	0.028699801140909642
1103	9	0.04754248899258329
1103	10-11	0.025342837043872635
1103	12-13	0.034722260709408204
1103	14-15	0.0395052802584388
1103	16-17	0.031883520435087576
1103	18-19	0.029226870942650862
1103	20-21	-0.010456575968802895
1103	22-23	0.022118743839399713
1103	24-25	-0.01717652127692304
1103	26-27	0.011155157173845964
1103	28-29	0.0156531716197037
1103	30-31	0.044975547232532165
1103	32-33	0.042040487873862276
1103	34-35	0.027965587433875783
1103	36-37	0.030506679144799875
1103	38-39	0.05026292591816883
1103	40-41	0.030634571242856623
1103	42-43	0.04399824376957184
1103	44-45	-0.01052618665988092
1103	46-47	-0.08390344291431973
1103	48-49	0.008976856938545552
1103	50-51	0.013760398731761825
1103	52-53	-0.035497087767872415
1103	54-55	-0.005685135874166747
1103	56-57	-0.0808910874499702
1103	58-59	-0.06832654561941354
1103	60-61	-0.07107069887992878
1103	62-63	-0.03902918977868097
1103	64-65	-0.07221667919996122
1103	66-67	-0.023950826165396677
1103	68-69	0.001960457319526654
1103	70-71	-0.018495740254586224
1103	72-73	-0.016982024950053187
1103	74-75	-0.05572608785934108
1103	76-77	-0.025264828604875333
1103	78-79	-0.026049021141730577
1103	80-81	-0.016942557263050162
1103	82-83	-0.006355014831505912
1103	84-85	0.006984993280404694
1103	86-87	0.006410372242257267
1103	88-89	-0.004520084956929793
1103	90-91	0.014373349866680485
1103	92-93	-0.005124391277401941
1103	94-95	-0.011860815883188991
1103	96-97	-0.029016116589922092
1103	98-99	-0.009636475250641041
1103	100-101	-0.06719230969825318
1104	1	-0.035440033988486164
1104	2	0.0012077285504830115
1104	3	-0.00892446710747663
1104	4	0.08442624706231783
1104	5	0.024371500358832066
1104	6	0.024391013649157856
1104	7	0.0136814039925639
1104	8	0.029607933656805585
1104	9	-0.03516393860851963
1104	10-11	-0.02032690606701948
1104	12-13	-0.015200318281820557
1104	14-15	-0.018038798078805485
1104	16-17	-0.01557281843732028
1104	18-19	0.006989641668667446
1104	20-21	-0.004655858241626731
1104	22-23	-0.042631176486963795
1104	24-25	0.007208088911319521
1104	26-27	-0.003087076180079862
1104	28-29	-0.010909034544667406
1104	30-31	-0.085364226367183
1104	32-33	-0.0748125229519232
1104	34-35	-0.0692637293049927
1104	36-37	-0.035027765088372576
1104	38-39	-0.032242825013618415
1104	40-41	-3.3366658858824394E-4
1104	42-43	-0.04952568943117086
1104	44-45	-0.013122079892752936
1104	46-47	-0.03393459054841941
1104	48-49	-0.06387014770808719
1104	50-51	0.033554948470737145
1104	52-53	-0.050043912627877774
1104	54-55	-0.03900311194914252
1104	56-57	-0.024335874005174674
1104	58-59	-0.03525825551179196
1104	60-61	-0.011463640301563771
1104	62-63	0.0021033550615712215
1104	64-65	-0.005511377686801211
1104	66-67	-0.019160532346987225
1104	68-69	-0.011413495259652962
1104	70-71	-0.0534117279144013
1104	72-73	0.004710619750824208
1104	74-75	0.006425632680119975
1104	76-77	0.019374178891325755
1104	78-79	-0.019459149981145174
1104	80-81	0.014745084623605464
1104	82-83	0.029847763803097394
1104	84-85	0.01913419551659956
1104	86-87	0.005672853578317927
1104	88-89	-0.006866220577983029
1104	90-91	-0.00409736920195769
1104	92-93	3.564821336929924E-4
1104	94-95	-0.017335257212614863
1104	96-97	-0.030394286608636634
1104	98-99	0.008973087325973239
1104	100-101	-0.002870872084695719
1105	1	-0.0526461709913022
1105	2	-0.06264695139771703
1105	3	-0.024733710120258934
1105	4	0.11193805094655573
1105	5	0.03066700195783767
1105	6	0.015486944794581348
1105	7	0.020192765391861656
1105	8	6.091166456982933E-4
1105	9	-0.02193070581229506
1105	10-11	-0.027346050162783797
1105	12-13	-0.022872482452356735
1105	14-15	-0.058250407038428875
1105	16-17	-0.02444964042020814
1105	18-19	-0.06725793671909486
1105	20-21	-0.11597426577377234
1105	22-23	-0.11881981582624235
1105	24-25	-0.08362545539152677
1105	26-27	-0.08535399134930088
1105	28-29	-0.08971375541779736
1105	30-31	-0.0734695462906032
1105	32-33	-0.0754853858256368
1105	34-35	-0.11394208899943692
1105	36-37	-0.09573095498215878
1105	38-39	-0.06851457886376267
1105	40-41	-0.07444819787839663
1105	42-43	-0.04605594702174898
1105	44-45	-0.06512142507845198
1105	46-47	-0.05897712505638708
1105	48-49	-0.056902353597415356
1105	50-51	-0.023349977241636566
1105	52-53	-0.02820355402598551
1105	54-55	-0.05631482973385005
1105	56-57	-0.04907829753808812
1105	58-59	-0.0364658879072266
1105	60-61	-0.04012341187755197
1105	62-63	-0.06370949476612253
1105	64-65	-0.06684572538809164
1105	66-67	-0.0694106370712646
1105	68-69	-0.04991910880158912
1105	70-71	-0.09070323303487271
1105	72-73	-0.09047020087217561
1105	74-75	-0.06929231683004389
1105	76-77	-0.06064831908794588
1105	78-79	-0.04336323314309709
1105	80-81	-0.03288252344385967
1105	82-83	-0.041250483205580224
1105	84-85	-0.06089066028137324
1105	86-87	-0.09449062861847324
1105	88-89	-0.046783043230611554
1105	90-91	-0.07590561352046876
1105	92-93	-0.12410652296588509
1105	94-95	-0.13701058722119797
1105	96-97	-0.11692068197811523
1105	98-99	-0.09703674795849437
1105	100-101	-0.13365641684444896
1106	1	-0.08903391337421596
1106	2	-0.0646203897167652
1106	3	-0.058730040551303375
1106	4	0.07963954304274523
1106	5	0.0033107772835734295
1106	6	-0.014733520023028746
1106	7	-0.011949650719024874
1106	8	-0.003921042880442371
1106	9	-0.03511606244105536
1106	10-11	-0.030397885689502857
1106	12-13	-0.027596235451248674
1106	14-15	-0.049895940277508544
1106	16-17	-0.021196897575180174
1106	18-19	-0.032802154074865086
1106	20-21	-0.032164605443135486
1106	22-23	-0.039940171557006465
1106	24-25	-0.02946140107997053
1106	26-27	-0.07857683713597652
1106	28-29	0.021195227356237467
1106	30-31	0.01222289804858434
1106	32-33	0.02721953789458098
1106	34-35	0.04861087592210822
1106	36-37	0.013736131551404185
1106	38-39	0.051115488232539974
1106	40-41	0.03872551146444181
1106	42-43	0.03854925017980548
1106	44-45	0.01701730184900896
1106	46-47	-0.013832401100557945
1106	48-49	-0.0052245073364858285
1106	50-51	0.02016278368636648
1106	52-53	-0.024865782648163304
1106	54-55	5.4251756594680955E-5
1106	56-57	0.00412237806757787
1106	58-59	0.011118996456346508
1106	60-61	0.019560402592240678
1106	62-63	0.025507677334601908
1106	64-65	-0.012267313980963479
1106	66-67	-0.03752252370054521
1106	68-69	-0.05131477626166259
1106	70-71	-0.037989478024165635
1106	72-73	-0.025890601508791633
1106	74-75	0.002285658231613752
1106	76-77	-0.01687754568512645
1106	78-79	0.0084941094077422
1106	80-81	-0.005514631410385107
1106	82-83	-0.006031535689515977
1106	84-85	-9.4914925206524E-4
1106	86-87	-0.010386139970506747
1106	88-89	0.011716199724475018
1106	90-91	0.026253239362667102
1106	92-93	0.04079445498267731
1106	94-95	-6.14246276029462E-5
1106	96-97	-0.04842142392026361
1106	98-99	-0.0326045008611473
1106	100-101	-0.009611464419236881
1107	1	-0.12830782933158957
1107	2	-0.09676861399279346
1107	3	-0.10449439659016946
1107	4	0.043245776194609675
1107	5	-0.05136631474071862
1107	6	-0.04024843106564191
1107	7	-0.05030931982495446
1107	8	-0.05890671768104028
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2216	4	-0.01913076531941016
2216	5	-0.07529674620484172
2216	6	-0.1042121698507259
2216	7	-0.07943734053046114
2216	8	-0.1329430241452627
2216	9	-0.15972540486141895
2216	10-11	-0.14936847541714826
2216	12-13	-0.1378284409243804
2216	14-15	-0.20261705954803233
2216	16-17	-0.1821578725200652
2216	18-19	-0.17227603868647634
2216	20-21	-0.15830951694348272
2216	22-23	-0.13127324439373922
2216	24-25	-0.14281823550750516
2216	26-27	-0.21317606421337842
2216	28-29	-0.11630496022492309
2216	30-31	-0.11090935911899891
2216	32-33	-0.15053249505311328
2216	34-35	-0.12369657304023463
2216	36-37	-0.12478318018727208
2216	38-39	-0.14416152455317643
2216	40-41	-0.20766279898290207
2216	42-43	-0.21666855709734278
2216	44-45	-0.17608788227624217
2216	46-47	-0.1799543046865253
2216	48-49	-0.19749307042742004
2216	50-51	-0.150619432748762
2216	52-53	-0.15980817538142134
2216	54-55	-0.1920060578753251
2216	56-57	-0.20379490770756092
2216	58-59	-0.2910250466253075
2216	60-61	-0.33693403461773386
2216	62-63	-0.3172220127179912
2216	64-65	-0.26297943208722074
2216	66-67	-0.2301243417163903
2216	68-69	-0.2241224272798874
2216	70-71	-0.24391483579375262
2216	72-73	-0.2561849472367683
2216	74-75	-0.23078008536614902
2216	76-77	-0.2039347667794189
2216	78-79	-0.21936934725209056
2216	80-81	-0.17118203002729615
2216	82-83	-0.17590425392157982
2216	84-85	-0.1776804361324551
2216	86-87	-0.14081213715567742
2216	88-89	-0.1599491171837073
2216	90-91	-0.17049843225688477
2216	92-93	-0.15679322814572672
2216	94-95	-0.19896208119851622
2216	96-97	-0.18100922301159983
2216	98-99	-0.23687907392955054
2216	100-101	-0.179263060268692
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41394.0
3	11632.0
4	12524.0
5	28844.0
6	109594.0
7	100611.0
8	78163.0
9	101971.0
10	153570.0
11	159294.0
12	124322.0
13	82920.0
14	56699.0
15	45909.0
16	39746.0
17	35491.0
18	33108.0
19	32391.0
20	33822.0
21	37832.0
22	47171.0
23	60071.0
24	75662.0
25	89000.0
26	103742.0
27	120976.0
28	142861.0
29	171575.0
30	208706.0
31	261990.0
32	339581.0
33	451309.0
34	592624.0
35	849608.0
36	1528336.0
37	3761872.0
38	7465872.0
39	3854722.0
40	13103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.692230552424533	18.25061587857451	16.687060789182162	38.37009277981879
2	16.205742877660224	20.57652034656921	55.27023942631909	7.947497349451477
3	12.776384330699909	21.880852934096183	48.072205908158644	17.270556827045265
4	17.33821631395362	29.661376273885367	40.12368514971954	12.876722262441472
5	18.21100240177945	33.86289985228423	37.535596071550806	10.390501674385517
6	16.16916313241088	35.36985880779791	36.405038995422224	12.055939064368989
7	12.931836467544619	16.628878424819124	57.724363851068674	12.714921256567582
8	13.79888490488996	21.6971195442316	47.92478248133221	16.57921306954623
9	16.270597696766618	22.837282325720185	45.38325963638323	15.508860341129974
10-11	18.085436288467363	31.197909614515453	37.064933604546034	13.651720492471153
12-13	18.32312909501465	22.753031661996836	43.21706441025393	15.706774832734585
14-15	18.74162098398513	26.45793072773538	41.093812310320885	13.7066359779586
16-17	19.578023304647882	27.539251214272596	38.5418527185047	14.340872762574822
18-19	19.430811002405797	28.111607679745443	35.59493135700938	16.86264996083938
20-21	21.627979600926142	27.79601699745732	34.75541071262217	15.820592688994372
22-23	20.814130710234803	30.22236177903871	33.66194769143833	15.301559819288164
24-25	20.72604938368847	29.97442760752017	32.389825088619936	16.909697920171425
26-27	21.030802319991405	30.196769263541878	32.21055728317441	16.561871133292307
28-29	21.64565128872111	29.95575789225619	31.653816029198232	16.74477478982447
30-31	20.867586629650887	31.891973733738094	30.312820258027877	16.927619378583138
32-33	20.795269339579605	30.235180185255768	31.678617492737395	17.290932982427236
34-35	21.85835883312648	30.734980850219312	30.06306992340647	17.34359039324773
36-37	21.955781728317607	31.07293461761159	29.992648427137453	16.97863522693335
38-39	22.231508476074236	28.769090232088445	31.75730774494547	17.242093546891844
40-41	22.217791783650288	30.74563325626561	29.257052507758747	17.77952245232536
42-43	22.298707481014414	30.43128856971525	29.886413260747315	17.383590688523025
44-45	22.532502327708713	29.719528026978647	30.218820350857357	17.529149294455284
46-47	20.78412722200864	30.8374483206039	29.96984161926463	18.40858283812283
48-49	21.296820371959303	30.502103334356622	30.362006138328468	17.839070155355607
50-51	21.08563253599046	30.542726519133524	31.021309410249742	17.350331534626275
52-53	23.512510927632963	28.861055799552155	29.190782205934784	18.435651066880094
54-55	22.54439608635349	29.015234511877946	30.02188992438558	18.41847947738298
56-57	23.06797071970869	29.10272884675809	29.942810030024308	17.886490403508915
58-59	22.24190533070237	29.950399359154993	29.381252220672422	18.42644308947022
60-61	22.600604221889682	29.56702589990867	28.879201934289455	18.953167943912188
62-63	23.21495193696598	28.96655631073261	29.432639471000112	18.385852281301297
64-65	22.82581237663472	29.085855464753468	29.729510550104177	18.358821608507636
66-67	23.197561972871963	28.980063946035816	28.834578811174083	18.987795269918138
68-69	23.755831285037424	28.16568311630232	29.53852975970096	18.539955838959298
70-71	22.3952520437325	28.093582825059155	30.138987913430622	19.372177217777725
72-73	23.195485124521664	27.944934220808804	29.641523017559575	19.21805763710996
74-75	24.285072715129246	28.035017818985292	29.50582887489619	18.174080590989274
76-77	22.434517944411088	28.426136724279853	29.754480651135356	19.384864680173703
78-79	22.08893423627556	28.808607395669938	29.497649528845077	19.604808839209422
80-81	22.47996015716324	28.089785041890554	30.549684841743897	18.880569959202308
82-83	22.558671733447703	29.219341111437195	28.897542883504972	19.324444271610126
84-85	22.290809351831577	28.979574537073592	29.26407303058214	19.465543080512692
86-87	21.870287958020594	30.13442324180725	29.979828692501243	18.015460107670915
88-89	23.278934692586045	27.36952067684027	31.124181697483564	18.22736293309012
90-91	23.41182152541271	27.982859368549917	29.422655161571008	19.18266394446637
92-93	22.892767913517993	27.88035740656006	30.085732773395208	19.14114190652674
94-95	23.568936216381008	28.44930863818155	28.868426829198896	19.113328316238547
96-97	23.040371755151277	28.181015807648834	29.947694180437413	18.830918256762477
98-99	25.073297610357525	27.150180607848995	29.1190911161608	18.657430665632685
100-101	25.27185034018625	27.56843386920594	28.47686984281561	18.682845947792202
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	44.0
1	158.0
2	340.0
3	546.5
4	840.5
5	1221.5
6	1727.5
7	2386.0
8	3230.5
9	4208.5
10	5403.5
11	6741.5
12	8363.0
13	10430.5
14	12746.5
15	15474.0
16	18630.5
17	22691.5
18	28071.5
19	34741.5
20	47444.0
21	65163.5
22	85236.5
23	109212.5
24	138613.5
25	183891.0
26	225623.5
27	313866.0
28	419097.0
29	562168.5
30	713549.0
31	724700.0
32	792529.5
33	935566.5
34	1128302.5
35	1144674.5
36	985267.0
37	1041567.0
38	1069655.5
39	1128480.0
40	1127754.0
41	952602.0
42	779728.5
43	635492.5
44	563635.0
45	492391.0
46	427637.0
47	388304.0
48	388499.5
49	379526.0
50	342078.0
51	316472.0
52	313581.0
53	302995.5
54	328484.5
55	295189.5
56	226948.5
57	232969.0
58	220281.0
59	168531.5
60	115005.0
61	76347.0
62	54268.5
63	40509.5
64	31365.0
65	25610.5
66	18831.5
67	12222.0
68	7214.0
69	4932.0
70	3477.0
71	5106.5
72	15663.5
73	32752.5
74	35139.0
75	22525.0
76	13435.5
77	27968.5
78	29271.5
79	8241.5
80	1966.0
81	490.5
82	172.0
83	91.0
84	64.0
85	59.0
86	41.0
87	30.5
88	21.0
89	11.5
90	7.5
91	4.5
92	5.5
93	8.5
94	8.0
95	9.0
96	7.5
97	6.0
98	5.0
99	6.0
100	20.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01439980897185457
2	0.016892979780897353
3	0.014134181427713565
4	0.005741283059328425
5	0.005564198029901087
6	0.006915636412372876
7	1.3980397060052982E-5
8	0.0
9	0.0032481122502856425
10-11	8.551342868399074E-4
12-13	6.174675368190067E-4
14-15	0.004646151956290941
16-17	0.005706332066678292
18-19	0.00687136515501604
20-21	0.0027261774267103313
22-23	0.008413868963975219
24-25	0.012428572986387103
26-27	0.014481361288038215
28-29	0.019432751913473646
30-31	0.015201351736630944
32-33	0.01717491778827509
34-35	0.01650618879556922
36-37	0.018759362788414426
38-39	0.026851682620008425
40-41	0.022182230001950732
42-43	0.030542507443862416
44-45	0.026350718392023197
46-47	0.028149529480416678
48-49	0.03799405907687065
50-51	0.04603278738640112
52-53	0.03919171309168186
54-55	0.03672883314293586
56-57	0.028741366289292254
58-59	0.038164153907767964
60-61	0.029822516995269686
62-63	0.03599952242963643
64-65	0.04125848179039303
66-67	0.05637362107848697
68-69	0.04800402337186859
70-71	0.04571822845254993
72-73	0.040242572937362504
74-75	0.042053034356639374
76-77	0.04025655333442256
78-79	0.04830227184248305
80-81	0.048213729327769385
82-83	0.05713322265208318
84-85	0.055602369174007384
86-87	0.04929721009992349
88-89	0.042803315665528884
90-91	0.03650281672379834
92-93	0.05907649784343055
94-95	0.0928111959493384
96-97	0.08513828802954598
98-99	0.07727431468326618
100-101	0.21612295815135904
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.1458618E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.73329066849012
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.12107762228187	11.215139626077777
2	8.517120215521226	2.5097041558749487
3	3.639386427758904	1.6086041428538957
4	2.1759789905796354	1.2823732382695
5	1.4850284229580255	1.0939677703205037
6	1.0502191107836574	0.9283910054867309
7	0.7532011180249087	0.7767991702584899
8	0.6014591961605957	0.7089178529816378
9	0.4982903983969182	0.6607311549209586
>10	3.94710022337049	11.889510984233363
>50	0.5797105562282798	6.006484684736142
>100	0.50802418225355	15.177499555964994
>500	0.06367569858960132	6.52079438993318
>1k	0.04885089720604385	14.246763048890445
>5k	0.005629293450081278	5.786191818100997
>10k+	0.005247646436516446	19.58812740109624
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA	166237	0.7746864220240092	No Hit
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA	152482	0.7105863015036662	No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT	137713	0.6417608067770255	No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC	100837	0.4699137661148542	No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT	94916	0.4423211224506629	No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA	85843	0.40003974160870936	No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG	82820	0.3859521615045293	No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA	81666	0.3805743687687623	No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT	73584	0.34291117908897956	No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG	66622	0.31046733764494994	No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA	66128	0.3081652322623945	No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG	61730	0.28766997017235685	No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA	61633	0.2872179373340818	No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA	59599	0.2777392281273659	No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG	58676	0.2734379259652229	No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA	51793	0.24136223497710804	No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT	50826	0.2368558869914176	No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT	47879	0.22312247694609225	No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA	47101	0.21949689397518518	No Hit
CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA	46340	0.2159505332542851	No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT	45239	0.2108197275332456	No Hit
CAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCA	42509	0.1980975662085974	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	41662	0.1941504341053091	No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC	38569	0.17973664473639447	No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG	36235	0.16885989582367328	No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT	35846	0.16704710433821973	No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC	34448	0.16053223930823504	No Hit
GTGAAATAAATGCTTTTTATTTGTCAGAAGACAGATTTTCAAATGTCTTT	34423	0.16041573599940126	No Hit
CTCAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCT	34115	0.15898041523456916	No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC	33090	0.15420377957238438	No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA	32732	0.15253545218988473	No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA	32119	0.14967879105728057	No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC	31600	0.14726018236589142	No Hit
CGCAGGATATTTTATTGAGACAAGGTGGGTCAACGGGAAGCTGAGCAGTG	31385	0.14625825390992095	No Hit
CAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTGTGTAGAAAT	30286	0.14113676845358822	No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA	29961	0.13962222543874914	No Hit
GTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCT	29728	0.13853641460041835	No Hit
TTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAGAATTG	29669	0.13826146679157064	No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT	29401	0.1370125513208726	No Hit
CGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAG	27665	0.12892256155545523	No Hit
AATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAA	27510	0.12820024104068584	No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG	27432	0.12783675071712447	No Hit
CAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTG	26626	0.12408068404032356	No Hit
GTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAAT	26161	0.12191372249601536	No Hit
TATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAAT	24874	0.11591613215725263	No Hit
TGTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAA	24692	0.11506798806894274	No Hit
CATAAATTAAGTTGAGAGCGCTTATTTGTAAGGTGGCTCTATTTCTCTTG	24371	0.11357208558351707	No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA	24357	0.11350684373057016	No Hit
CGAACGTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGA	24262	0.11306413115700183	No Hit
GTTTCTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGT	24134	0.11246763421577288	No Hit
TTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGAT	23746	0.1106595028626727	No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT	23687	0.110384555053825	No Hit
CTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAGAATTGTGACTAGA	22851	0.10648868440642358	No Hit
CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT	22726	0.10590616786225468	No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT	22604	0.10533763171514587	No Hit
GTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTG	22435	0.10455006934742955	No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG	21970	0.10238310780312135	No Hit
GATTTATTTACCTGAAATCATGTCTGTGCAATACCTGTTACCAGCACAGA	21792	0.10155360424422485	No Hit
CTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTC	21495	0.10016954493527962	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4446410295388082E-4	0.0	0.0	0.0	0.0
2	1.4446410295388082E-4	0.0	0.0	0.0	0.0
3	1.4446410295388082E-4	0.0	0.0	1.3980397060052982E-5	0.0
4	1.4446410295388082E-4	0.0	0.0	1.3980397060052982E-5	0.0
5	1.4446410295388082E-4	0.0	0.0	1.8640529413403976E-5	0.0
6	1.491242353072318E-4	0.0	0.0	2.330066176675497E-5	0.0
7	1.491242353072318E-4	0.0	0.0	2.330066176675497E-5	0.0
8	1.491242353072318E-4	0.0	0.0	2.330066176675497E-5	0.0
9	1.491242353072318E-4	0.0	0.0	2.330066176675497E-5	0.0
10-11	1.491242353072318E-4	0.0	0.0	2.330066176675497E-5	0.0
12-13	1.491242353072318E-4	0.0	0.0	2.330066176675497E-5	0.0
14-15	1.5145430148390731E-4	0.0	0.0	2.330066176675497E-5	0.0
16-17	1.607745661906093E-4	0.0	0.0	2.330066176675497E-5	0.0
18-19	1.677647647206358E-4	0.0	0.0	3.495099265013246E-5	0.0
20-21	1.9339549266406624E-4	0.0	0.0	5.126145588686093E-5	0.0
22-23	2.563072794343047E-4	0.0	0.0	6.75719191235894E-5	0.0
24-25	3.4484979414797356E-4	0.0	0.0	7.922225000696689E-5	0.0
26-27	4.776635662184769E-4	0.0	0.0	8.854251471366888E-5	4.660132353350994E-6
28-29	7.54941441242861E-4	0.0	0.0	9.320264706701989E-5	4.660132353350994E-6
30-31	0.0013444481839417618	0.0	0.0	9.786277942037087E-5	4.660132353350994E-6
32-33	0.002479190411982729	0.0	0.0	1.0019284559704637E-4	4.660132353350994E-6
34-35	0.004278001500376213	0.0	0.0	1.1650330883377484E-4	4.660132353350994E-6
36-37	0.006908646213842849	0.0	0.0	1.5145430148390731E-4	4.660132353350994E-6
38-39	0.011184317648042386	0.0	0.0	1.7009483089731127E-4	4.660132353350994E-6
40-41	0.01694657130296089	0.0	0.0	1.7941509560401327E-4	4.660132353350994E-6
42-43	0.02355929911236595	0.0	0.0	2.469870147276027E-4	4.660132353350994E-6
44-45	0.032306367539605765	0.0	0.0	2.586373456109802E-4	4.660132353350994E-6
46-47	0.04246778613608761	0.0	0.0	2.6562754414100666E-4	4.660132353350994E-6
48-49	0.054255590923888944	0.0	0.0	2.7261774267103313E-4	4.660132353350994E-6
50-51	0.06746240601328567	0.0	0.0	2.842680735544106E-4	4.660132353350994E-6
52-53	0.08408276805151199	0.0	0.0	2.865981397310861E-4	4.660132353350994E-6
54-55	0.10572675276665067	0.0	0.0	3.0756873532116564E-4	4.660132353350994E-6
56-57	0.12975905531288176	0.0	0.0	3.122288676745166E-4	4.660132353350994E-6
58-59	0.15650588495493978	0.0	0.0	3.634903235613775E-4	4.660132353350994E-6
60-61	0.18536608461924248	0.0	0.0	3.867909853281325E-4	4.660132353350994E-6
62-63	0.22116056122533148	2.330066176675497E-6	0.0	3.89121051504808E-4	4.660132353350994E-6
64-65	0.26243069334660785	4.660132353350994E-6	0.0	3.9844131621151E-4	4.660132353350994E-6
66-67	0.31092170054940166	4.660132353350994E-6	0.0	4.03101448564861E-4	4.660132353350994E-6
68-69	0.3689916098045084	4.660132353350994E-6	0.0	4.1009164709488745E-4	4.660132353350994E-6
70-71	0.4328563936410071	4.660132353350994E-6	0.0	4.1009164709488745E-4	4.660132353350994E-6
72-73	0.508488011669717	4.660132353350994E-6	0.0	4.1009164709488745E-4	4.660132353350994E-6
74-75	0.608238144693195	4.660132353350994E-6	0.0	4.17081845624914E-4	4.660132353350994E-6
76-77	0.7206102461957242	4.660132353350994E-6	0.0	4.287321765082914E-4	4.660132353350994E-6
78-79	0.8463312968244274	4.660132353350994E-6	0.0	4.4504263974501997E-4	4.660132353350994E-6
80-81	0.9910214161974458	4.660132353350994E-6	0.0	4.7533350004180135E-4	4.660132353350994E-6
82-83	1.160275093204977	4.660132353350994E-6	0.0	4.7999363239515237E-4	9.320264706701988E-6
84-85	1.3972964148949387	4.660132353350994E-6	0.0	4.7999363239515237E-4	9.320264706701988E-6
86-87	1.6413312357766934	4.660132353350994E-6	0.0	4.823236985718279E-4	9.320264706701988E-6
88-89	1.92005841196297	4.660132353350994E-6	0.0	5.009642279852318E-4	9.320264706701988E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	45485	0.0	88.661674	1
GCGTGTT	49385	0.0	81.034325	2
CGTGTTT	65665	0.0	60.840794	3
GTAAACG	6050	0.0	53.84717	1
CGAACGT	4610	0.0	51.81568	1
TAAACGC	6640	0.0	49.062214	2
GTGTTTT	82105	0.0	48.09417	4
GATCGGG	560	0.0	42.401	1
GTTTTTT	281485	0.0	40.53408	1
TCGAACG	1985	0.0	39.235073	2
CTTACGG	4435	0.0	36.834854	1
TTACGGT	4355	0.0	36.747925	2
TCGCAGG	5725	0.0	36.332035	2
TCATCAA	46240	0.0	35.48308	2
AAACGCT	9225	0.0	35.467518	3
TAGATAT	29555	0.0	35.268055	3
GAACGTT	6895	0.0	34.919243	2
CGCAGGA	8890	0.0	34.66866	1
ATCAATA	47220	0.0	34.63257	4
GTAGATA	30380	0.0	34.31135	2
>>END_MODULE