FastQCFastQC Report
Fri 27 May 2016
SRR2032137_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2032137_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21458618
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC991650.46212202482005127No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT430250.2005021945029265No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT423380.1973006835761744No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA346040.1612592199553578No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT343900.16026195163174067No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA335020.156123754101965No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT317860.1481269669836147No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC312580.14566641710104536No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC294630.13730147952678032No Hit
CTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA294260.13712905462970634No Hit
CTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCA290180.13522772062953914No Hit
CTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGA249300.11617709956904029No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT240280.11197366018631767No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG238390.11109289517153434No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA227560.10604597183285522No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT223530.10416793849445476No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA220140.10258815362666877No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA218490.10181923178836587No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC216710.1009897282294694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA164250.053.8320051
CCCGATC8100.046.282661
CGACCGT1500.042.6703531
CCAAACG215350.041.0293858
CCGAAAC216250.040.8938832
TACGCAT54400.039.115239
TCCACTC510050.038.573932
CTCCACT536250.037.665861
TCGTGTT26650.037.0416222
CTAACGC16000.036.847092
ACTCGTC176950.036.6563842-43
CCACTCA551450.035.7442973
CTAACGA180600.035.14235394-95
CTACGCA60650.034.933828
CGCCTAT5500.034.9121061
ACTCATG572000.034.7872245
TCGTCTA186650.034.7269244-45
CTCGTAC40250.034.3028761
CTCTACG62200.034.0813876
TCATGAG612850.033.52167
Produced by FastQC (version 0.11.5)