##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2032137_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21458618 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.78044042724466 34.0 31.0 34.0 31.0 34.0 2 32.91358702596784 34.0 31.0 34.0 31.0 34.0 3 32.943973605383164 34.0 31.0 34.0 31.0 34.0 4 36.34733644077172 37.0 37.0 37.0 35.0 37.0 5 36.316599978619315 37.0 37.0 37.0 35.0 37.0 6 36.21784734692607 37.0 37.0 37.0 35.0 37.0 7 36.15232779669222 37.0 37.0 37.0 35.0 37.0 8 36.15756270977003 37.0 37.0 37.0 35.0 37.0 9 37.97157799258088 39.0 39.0 39.0 35.0 39.0 10-11 38.06249113992337 39.0 39.0 39.0 35.0 39.0 12-13 37.98266617169847 39.0 38.5 39.0 35.0 39.0 14-15 39.48807754068785 41.0 39.0 41.0 36.0 41.0 16-17 39.455958370664874 41.0 39.0 41.0 36.0 41.0 18-19 39.432085980560345 41.0 39.0 41.0 36.5 41.0 20-21 39.460180776786274 41.0 39.0 41.0 36.0 41.0 22-23 39.45732702823639 41.0 39.0 41.0 37.0 41.0 24-25 39.49911995730574 41.0 39.0 41.0 37.0 41.0 26-27 39.48625831821975 41.0 39.0 41.0 37.0 41.0 28-29 29.6602358315899 21.5 20.5 41.0 19.5 41.0 30-31 30.701424574499626 26.5 23.0 41.0 20.5 41.0 32-33 37.69418333463972 38.5 37.0 40.0 34.0 41.0 34-35 38.97747096294831 40.0 38.5 41.0 35.5 41.0 36-37 39.21884214537954 41.0 39.0 41.0 36.0 41.0 38-39 39.20888251051396 41.0 39.0 41.0 36.0 41.0 40-41 39.13519701035733 41.0 39.0 41.0 35.0 41.0 42-43 39.0723974162735 41.0 39.0 41.0 35.0 41.0 44-45 39.019604897202605 41.0 39.0 41.0 35.0 41.0 46-47 38.953881396276316 41.0 38.5 41.0 35.0 41.0 48-49 38.897049521082856 40.0 38.0 41.0 35.0 41.0 50-51 38.87585454011997 40.0 38.0 41.0 35.0 41.0 52-53 38.800051825331906 40.0 38.0 41.0 35.0 41.0 54-55 38.70692665296526 40.0 38.0 41.0 35.0 41.0 56-57 38.56640297152407 40.0 38.0 41.0 34.5 41.0 58-59 38.4095786830261 40.0 37.5 41.0 34.0 41.0 60-61 38.18880193030138 40.0 37.0 41.0 34.0 41.0 62-63 37.88523787971807 39.0 36.5 41.0 34.0 41.0 64-65 37.52550413544805 39.0 36.0 41.0 33.5 41.0 66-67 37.15285133926146 38.5 35.5 40.5 33.0 41.0 68-69 36.73312263166248 37.5 35.0 40.0 32.5 41.0 70-71 36.405463063837566 37.0 35.0 39.0 32.0 41.0 72-73 36.10386761626494 36.5 35.0 39.0 32.0 41.0 74-75 35.72144121769631 36.0 35.0 39.0 32.0 40.5 76-77 35.36569279531422 36.0 35.0 37.5 32.0 39.5 78-79 34.98576704706706 35.0 35.0 37.0 31.5 39.0 80-81 34.602813144816686 35.0 34.0 37.0 31.0 39.0 82-83 34.33586328812042 35.0 34.0 36.0 31.0 38.0 84-85 34.10744629500371 35.0 34.0 36.0 31.0 37.0 86-87 33.8686061469569 35.0 34.0 36.0 31.0 37.0 88-89 33.731565378534626 35.0 34.0 35.0 31.0 36.5 90-91 33.679727324471685 35.0 34.0 35.0 31.0 36.0 92-93 33.674192461974954 35.0 34.0 35.0 31.0 36.0 94-95 33.57083862530197 35.0 34.0 35.0 31.0 36.0 96-97 33.48792033112291 35.0 34.0 35.0 31.0 36.0 98-99 33.35538351071817 35.0 34.0 35.0 31.0 36.0 100-101 33.08206448802994 35.0 34.0 35.0 30.5 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09698259112465024 1101 2 0.11290419658727302 1101 3 0.09567859596005235 1101 4 0.07324636351506086 1101 5 0.06894637520031921 1101 6 0.09977252405290216 1101 7 0.09098172906002588 1101 8 0.05331312124777554 1101 9 0.07290794229237463 1101 10-11 0.06793791957377948 1101 12-13 0.0754776919878779 1101 14-15 0.15230677731869946 1101 16-17 0.06667004784665664 1101 18-19 0.14006359165129822 1101 20-21 0.13398017192989187 1101 22-23 0.13530372471453944 1101 24-25 0.13752696297544276 1101 26-27 0.13126072084774165 1101 28-29 9.469976278869026 1101 30-31 8.246830226607383 1101 32-33 1.5016608874687165 1101 34-35 0.37392290971445874 1101 36-37 0.17194349693072297 1101 38-39 0.16684109564085503 1101 40-41 0.12365202296977884 1101 42-43 0.13311427915052576 1101 44-45 0.11396680110733115 1101 46-47 0.13467627883338906 1101 48-49 0.12443352997738089 1101 50-51 0.16465738196513513 1101 52-53 0.14393717255524763 1101 54-55 0.13538209160586945 1101 56-57 0.12969346126052983 1101 58-59 0.20648843715636644 1101 60-61 0.18452387343436527 1101 62-63 0.25015376483928975 1101 64-65 0.29716363041638516 1101 66-67 0.3090603971119137 1101 68-69 0.32699644074084233 1101 70-71 0.34312521706311827 1101 72-73 0.3069426697688158 1101 74-75 0.3227452408988398 1101 76-77 0.3511463361518352 1101 78-79 0.3800460143671245 1101 80-81 0.38147339715101936 1101 82-83 0.33857294376473135 1101 84-85 0.2983590851811897 1101 86-87 0.283712701792588 1101 88-89 0.26640981651802775 1101 90-91 0.2577388589847729 1101 92-93 0.21636746664940176 1101 94-95 0.1922976353183401 1101 96-97 0.1633627789979002 1101 98-99 0.20862854971422706 1101 100-101 0.16248026830975704 1102 1 0.061925060294839795 1102 2 0.07753416257961021 1102 3 0.05931721838016557 1102 4 0.049239476233189805 1102 5 0.05400616575455075 1102 6 0.07318670796350801 1102 7 0.07649248508292317 1102 8 0.04766429478218015 1102 9 0.05773100322706881 1102 10-11 0.06840588650536006 1102 12-13 0.09103828066628239 1102 14-15 0.1087653751133999 1102 16-17 0.07919280447637078 1102 18-19 0.1399449927448586 1102 20-21 0.1388831751057964 1102 22-23 0.12785453245696488 1102 24-25 0.11861146624556795 1102 26-27 0.10691043833547553 1102 28-29 9.46233740376384 1102 30-31 8.309111931588543 1102 32-33 1.5518815553928533 1102 34-35 0.3869666318403233 1102 36-37 0.157016690928117 1102 38-39 0.1309125505140898 1102 40-41 0.10526424724352523 1102 42-43 0.10977823097195483 1102 44-45 0.11571116861897224 1102 46-47 0.094277790421323 1102 48-49 0.08225946184703048 1102 50-51 0.05982600509704383 1102 52-53 0.0833989058704887 1102 54-55 0.09894140801547024 1102 56-57 0.1379488691390236 1102 58-59 0.16888875118645075 1102 60-61 0.1545254882608731 1102 62-63 0.21945510687586278 1102 64-65 0.25532437482552695 1102 66-67 0.2618926438238205 1102 68-69 0.20239533711631452 1102 70-71 0.26374904260856624 1102 72-73 0.2655244219317723 1102 74-75 0.2907965528076346 1102 76-77 0.3267718115717031 1102 78-79 0.32378035669339056 1102 80-81 0.3102781238023553 1102 82-83 0.28350971007361636 1102 84-85 0.24771999634830166 1102 86-87 0.22560488566777792 1102 88-89 0.21159793503640145 1102 90-91 0.20621430076960223 1102 92-93 0.16578370146815757 1102 94-95 0.15182712862739578 1102 96-97 0.11308188010020359 1102 98-99 0.13038349196163068 1102 100-101 0.08105044058627442 1103 1 0.02529796583593935 1103 2 0.050390103197976543 1103 3 0.04959916385756458 1103 4 0.042523985390396035 1103 5 0.033614513385735734 1103 6 0.058205918932571876 1103 7 0.045878855370546034 1103 8 0.036991999811711196 1103 9 0.050037325487316764 1103 10-11 -4.159201364331011E-4 1103 12-13 0.05041184165973789 1103 14-15 0.06999283516066157 1103 16-17 0.05865012285266147 1103 18-19 0.08764969928709831 1103 20-21 0.06134995136962118 1103 22-23 0.0604886940437126 1103 24-25 0.075247441003782 1103 26-27 0.02680176209002383 1103 28-29 9.400067172454499 1103 30-31 8.201946144595933 1103 32-33 1.3989948735026445 1103 34-35 0.30703164725373 1103 36-37 0.07822055664881589 1103 38-39 0.06912317450881034 1103 40-41 0.057503893758436675 1103 42-43 0.0809065161071203 1103 44-45 0.08177774635750978 1103 46-47 0.06502071951793909 1103 48-49 0.060852581213026724 1103 50-51 0.0680993806058865 1103 52-53 0.04052796820564453 1103 54-55 0.04960019904525126 1103 56-57 0.09556348425602579 1103 58-59 0.11536354047813546 1103 60-61 0.08358938147177497 1103 62-63 0.14154414179916586 1103 64-65 0.1651263575501858 1103 66-67 0.22131676046777926 1103 68-69 0.23652840116612595 1103 70-71 0.26944600626406157 1103 72-73 0.2258587474216185 1103 74-75 0.2625229627518024 1103 76-77 0.28791523053493506 1103 78-79 0.25625416786535027 1103 80-81 0.2395309899796345 1103 82-83 0.248743946149645 1103 84-85 0.16582865170906302 1103 86-87 0.19209816182987538 1103 88-89 0.2343718173338729 1103 90-91 0.19706336057745943 1103 92-93 0.16449613451042921 1103 94-95 0.1458213279456615 1103 96-97 0.12505731292039002 1103 98-99 0.13110007158716286 1103 100-101 0.012091322546190497 1104 1 0.029201875216571693 1104 2 0.021695431603291127 1104 3 0.02397504904245551 1104 4 0.038100691385324126 1104 5 0.011582312187798038 1104 6 0.05404317100303757 1104 7 0.06585180731285334 1104 8 0.03397345294003884 1104 9 0.02783193670919104 1104 10-11 0.028347634634855012 1104 12-13 0.0418881020582802 1104 14-15 0.024656445826984452 1104 16-17 0.040263686542296284 1104 18-19 0.10195881673779184 1104 20-21 0.05032383248503436 1104 22-23 0.05552187918695495 1104 24-25 0.056490643560167086 1104 26-27 0.04312322057202067 1104 28-29 9.3937209268353 1104 30-31 8.226302389045298 1104 32-33 1.3564981765815673 1104 34-35 0.2730302560334721 1104 36-37 0.05273745905492433 1104 38-39 0.08080355122788063 1104 40-41 -0.006900673336375007 1104 42-43 0.016994319689786153 1104 44-45 0.02772294545554388 1104 46-47 0.042192881061687615 1104 48-49 0.037988214523167585 1104 50-51 0.035294335182314285 1104 52-53 -0.00387663399495608 1104 54-55 0.06604498602634834 1104 56-57 0.09877243577055594 1104 58-59 0.08092023420124406 1104 60-61 0.06347839301689362 1104 62-63 0.13821643160842 1104 64-65 0.19409781065427723 1104 66-67 0.14120336081811757 1104 68-69 0.21650088349881003 1104 70-71 0.23548855400269986 1104 72-73 0.19001521756381123 1104 74-75 0.20815956422357118 1104 76-77 0.22989659263498652 1104 78-79 0.22426735109337415 1104 80-81 0.2341393149337776 1104 82-83 0.22722808369093173 1104 84-85 0.16978301870066304 1104 86-87 0.14450931650272736 1104 88-89 0.11051573032124651 1104 90-91 0.06113018248682067 1104 92-93 0.07353386888274116 1104 94-95 0.0417285944788901 1104 96-97 5.609103864543385E-4 1104 98-99 0.015003337341703116 1104 100-101 -0.05961552845783302 1105 1 0.007866707547336205 1105 2 0.006717412969891257 1105 3 -0.008823191241759787 1105 4 0.0026014117235888534 1105 5 -1.8355113713397486E-4 1105 6 0.021021128385356747 1105 7 0.022179697742402027 1105 8 0.014538685208677293 1105 9 0.0032120018093522162 1105 10-11 0.007595980207902642 1105 12-13 -0.0020541435448677703 1105 14-15 0.003054830144044729 1105 16-17 -0.02087787685371012 1105 18-19 0.0627789743533782 1105 20-21 0.01741292442882525 1105 22-23 0.04182924199641036 1105 24-25 0.018068216839409956 1105 26-27 0.017962755493776683 1105 28-29 9.371800791983922 1105 30-31 8.176299662908061 1105 32-33 1.3864267030633926 1105 34-35 0.23363500240468937 1105 36-37 0.02582190198733514 1105 38-39 0.02938732293117141 1105 40-41 -0.009152654107097646 1105 42-43 -0.00852665577084366 1105 44-45 -0.020203180440113044 1105 46-47 0.015075443864255078 1105 48-49 -0.052253735763926557 1105 50-51 -0.021986854505520625 1105 52-53 -0.055205623966521955 1105 54-55 -0.006225784210279528 1105 56-57 0.02997938044470061 1105 58-59 0.041656558263092336 1105 60-61 0.04627634618665155 1105 62-63 0.1115175202806924 1105 64-65 0.12538325551366825 1105 66-67 0.14510274535686563 1105 68-69 0.14255124484347448 1105 70-71 0.2081644852695419 1105 72-73 0.14598862798122525 1105 74-75 0.183661956515337 1105 76-77 0.1889941775983388 1105 78-79 0.1729442677524986 1105 80-81 0.16641860792613272 1105 82-83 0.13447969962328443 1105 84-85 0.13774375822551832 1105 86-87 0.10316508549055214 1105 88-89 0.08199609283331455 1105 90-91 0.027549712889765487 1105 92-93 0.042125348594076684 1105 94-95 0.02403128778260566 1105 96-97 -0.007336444320777957 1105 98-99 -0.015221231210347241 1105 100-101 -0.05907928469522261 1106 1 -0.029064735113024653 1106 2 -0.014044968183462458 1106 3 0.006279275194586376 1106 4 0.023732127182285012 1106 5 -0.0057536732612035735 1106 6 -0.0025915515354597574 1106 7 -0.0011596662117909773 1106 8 -0.00786956020182572 1106 9 0.0014364086764544481 1106 10-11 -0.020299444504004782 1106 12-13 0.013145627195250142 1106 14-15 -0.018361602749280337 1106 16-17 -0.05645917062238226 1106 18-19 -0.0215013061071474 1106 20-21 -0.045371902253677376 1106 22-23 -0.07962908480314468 1106 24-25 -0.09994438014240359 1106 26-27 -0.10572309012503212 1106 28-29 9.276707594244556 1106 30-31 8.164823736901749 1106 32-33 1.3791501039577128 1106 34-35 0.18104195380507093 1106 36-37 -0.029385000696130703 1106 38-39 -0.09434208363258989 1106 40-41 -0.0843235592690661 1106 42-43 -0.13996520684580105 1106 44-45 -0.10310919252057005 1106 46-47 -0.07763019551644135 1106 48-49 -0.08679544641538683 1106 50-51 -0.08934016970018632 1106 52-53 -0.05654574003811064 1106 54-55 -0.03404351239650083 1106 56-57 0.007646240672016802 1106 58-59 0.022233032865649704 1106 60-61 0.026938881184541685 1106 62-63 0.03235130689594001 1106 64-65 0.09451620908653524 1106 66-67 0.1026541597125572 1106 68-69 0.10170339822876429 1106 70-71 0.15611540506914423 1106 72-73 0.15480670355957926 1106 74-75 0.17081594932109567 1106 76-77 0.1698954551932843 1106 78-79 0.20554444322934984 1106 80-81 0.20531874635247505 1106 82-83 0.17066522514485882 1106 84-85 0.1738253805891219 1106 86-87 0.12176724113027859 1106 88-89 0.08438074391087724 1106 90-91 0.09104704711421618 1106 92-93 0.07406234328671246 1106 94-95 0.03371896014702003 1106 96-97 0.004322999323981946 1106 98-99 0.03582594725637733 1106 100-101 -0.08894551669174433 1107 1 -0.10255300476479334 1107 2 -0.06921744574481892 1107 3 -0.07592024128175012 1107 4 -0.024180092641358897 1107 5 -0.03230393767363182 1107 6 -0.02663845728283576 1107 7 -0.02432831085542375 1107 8 -0.06329699711200476 1107 9 -0.06803461502116193 1107 10-11 -0.06620011702969464 1107 12-13 -0.05589264383614534 1107 14-15 -0.07449852965912385 1107 16-17 -0.10140892286292313 1107 18-19 -0.024461457597851677 1107 20-21 -0.052573175072495815 1107 22-23 -0.09033070538581 1107 24-25 -0.08748804879473937 1107 26-27 -0.08285165258801186 1107 28-29 9.27302614148698 1107 30-31 8.106930077316559 1107 32-33 1.2610263252447709 1107 34-35 0.13628546072255432 1107 36-37 -0.15069262229171443 1107 38-39 -0.11492292139173088 1107 40-41 -0.16184236083611836 1107 42-43 -0.1449064677249865 1107 44-45 -0.17067753661420682 1107 46-47 -0.14634229338791727 1107 48-49 -0.15996720598337077 1107 50-51 -0.12862714979976175 1107 52-53 -0.14927804876470674 1107 54-55 -0.1380179108777 1107 56-57 -0.11721254272366366 1107 58-59 -0.1134575840315506 1107 60-61 -0.10933720858895413 1107 62-63 -0.0791561227662001 1107 64-65 -0.0719411872568898 1107 66-67 -0.027612796871935075 1107 68-69 0.024478712492047805 1107 70-71 0.003510734269866589 1107 72-73 0.009081412247702758 1107 74-75 0.03724792918715991 1107 76-77 0.025445641217075377 1107 78-79 0.05890427177059365 1107 80-81 0.06775027039717685 1107 82-83 0.018349033420918204 1107 84-85 -0.0016705793596685226 1107 86-87 -0.022921767915420332 1107 88-89 -0.015966739001804342 1107 90-91 -0.02224343174632537 1107 92-93 -0.0859524705292074 1107 94-95 -0.07717370613391239 1107 96-97 -0.08060317796205396 1107 98-99 -0.08524474712003638 1107 100-101 -0.1591569436247724 1108 1 -0.08735385444557409 1108 2 -0.0730475509042563 1108 3 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-0.18191561488470143 2214 72-73 -0.1756093909492904 2214 74-75 -0.1984791786005502 2214 76-77 -0.2568017153871267 2214 78-79 -0.29518649843178224 2214 80-81 -0.2504740550517326 2214 82-83 -0.21230201365864332 2214 84-85 -0.17321755017897544 2214 86-87 -0.15649931959239183 2214 88-89 -0.11250014744390313 2214 90-91 -0.07042466587546414 2214 92-93 -0.11300429295044268 2214 94-95 -0.08001712906954594 2214 96-97 -0.05715894117147258 2214 98-99 -0.04125969514714001 2214 100-101 -0.09035020080695944 2215 1 -0.07390000931542318 2215 2 -0.08386929207902227 2215 3 -0.10026142456186449 2215 4 -0.05388061123630905 2215 5 -0.01717251547476195 2215 6 -0.21656079831749508 2215 7 -0.09578586125454791 2215 8 -0.08545455503115562 2215 9 -0.06995140557541646 2215 10-11 -0.06406841247144257 2215 12-13 -0.04072529510173695 2215 14-15 -0.15543823562268955 2215 16-17 -0.11151084021216917 2215 18-19 -0.07411233018524399 2215 20-21 -0.0889666171361938 2215 22-23 -0.09407605351377413 2215 24-25 -0.11153688060264244 2215 26-27 -0.0915421809214294 2215 28-29 -9.373512857307496 2215 30-31 -8.256236482532142 2215 32-33 -1.6130010663344905 2215 34-35 -0.46980486451580106 2215 36-37 -0.19989769979667216 2215 38-39 -0.1488590185838703 2215 40-41 -0.17026975751771545 2215 42-43 -0.17395995764689332 2215 44-45 -0.1812825614246023 2215 46-47 -0.26478005198147514 2215 48-49 -0.16465484387830287 2215 50-51 -0.12526545643057574 2215 52-53 -0.15754685499462795 2215 54-55 -0.16299814794587064 2215 56-57 -0.19492447949493652 2215 58-59 -0.2387296287174081 2215 60-61 -0.19854167895449137 2215 62-63 -0.18444364209096165 2215 64-65 -0.253017494644844 2215 66-67 -0.32535693032552615 2215 68-69 -0.24924940088599357 2215 70-71 -0.2573845901979439 2215 72-73 -0.2754135721033748 2215 74-75 -0.33579842793432135 2215 76-77 -0.3363577225429424 2215 78-79 -0.3165954971653946 2215 80-81 -0.308418990875829 2215 82-83 -0.30415484364382905 2215 84-85 -0.2720362058353416 2215 86-87 -0.27688233658356154 2215 88-89 -0.2006929282719767 2215 90-91 -0.17313230511064148 2215 92-93 -0.16731531014596612 2215 94-95 -0.15574487372580137 2215 96-97 -0.13794761690368063 2215 98-99 -0.11975424744282037 2215 100-101 -0.20221279891833888 2216 1 -0.20928515603777953 2216 2 -0.17188502410123618 2216 3 -0.17228721268694613 2216 4 -0.14130721889494424 2216 5 -0.10289333623970975 2216 6 -0.1408741647563545 2216 7 -0.12440716196353208 2216 8 -0.12052367663043384 2216 9 -0.19431286306852513 2216 10-11 -0.19929831556856925 2216 12-13 -0.19949679786622454 2216 14-15 -0.25191797003054006 2216 16-17 -0.2729156793147567 2216 18-19 -0.2669138790882073 2216 20-21 -0.3018194688489615 2216 22-23 -0.26287924933372864 2216 24-25 -0.29158883911340183 2216 26-27 -0.3099286107667538 2216 28-29 -9.474270828880353 2216 30-31 -8.455235743678859 2216 32-33 -1.9002458159416875 2216 34-35 -0.7280493702670938 2216 36-37 -0.4014563719462103 2216 38-39 -0.37707337198744284 2216 40-41 -0.32180424727256707 2216 42-43 -0.35036577872168095 2216 44-45 -0.31248873471651706 2216 46-47 -0.3122327369137352 2216 48-49 -0.38197487107241557 2216 50-51 -0.36731449372405933 2216 52-53 -0.37278101193595603 2216 54-55 -0.4013377328635741 2216 56-57 -0.4014447212300212 2216 58-59 -0.41796053654886123 2216 60-61 -0.4066472988613228 2216 62-63 -0.394694393804528 2216 64-65 -0.4448631277722441 2216 66-67 -0.44996469786357807 2216 68-69 -0.4176555333450409 2216 70-71 -0.47114408114826745 2216 72-73 -0.5082175416673209 2216 74-75 -0.5286524739745886 2216 76-77 -0.5110384025962205 2216 78-79 -0.5175050316785672 2216 80-81 -0.521938603504374 2216 82-83 -0.5131203456664082 2216 84-85 -0.45776243497511615 2216 86-87 -0.4558780595981986 2216 88-89 -0.43212051434425547 2216 90-91 -0.433400677997966 2216 92-93 -0.4546933929854262 2216 94-95 -0.4355235740191077 2216 96-97 -0.4587555194521187 2216 98-99 -0.4495383150506811 2216 100-101 -0.5174702170303149 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1005.0 3 390.0 4 498.0 5 723.0 6 1261.0 7 2389.0 8 3558.0 9 4053.0 10 5179.0 11 7238.0 12 8720.0 13 9098.0 14 10634.0 15 12166.0 16 13847.0 17 14722.0 18 16007.0 19 17839.0 20 20465.0 21 23979.0 22 28580.0 23 35945.0 24 45171.0 25 58107.0 26 76042.0 27 100317.0 28 129803.0 29 165168.0 30 215436.0 31 285793.0 32 392970.0 33 568588.0 34 894429.0 35 1509611.0 36 2944710.0 37 6401569.0 38 5921189.0 39 1503218.0 40 8201.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.943464722915564 11.216236949070097 6.92010524891211 58.920193079102226 2 16.567014331407577 15.528801407584576 47.695162276166705 20.20902198484114 3 20.64287178233006 21.569021826102688 24.250387420103195 33.53771897146405 4 20.710074282691032 31.333446513400727 21.4739325535963 26.482546650311946 5 20.47510230665991 36.076619130766204 24.8468727308274 18.60140583174649 6 17.10967706320574 36.73463907027146 26.949803914730403 19.205879951792394 7 14.655516703275337 23.9414141333177 42.52949159600542 18.87357756740154 8 16.327398157700557 22.462625505519508 33.807111902546566 27.402864434233372 9 16.07570440929607 23.570436828690458 36.34612909368162 24.007729668331855 10-11 20.69066842981221 34.16374717141616 24.934662614339842 20.210921784431786 12-13 20.642512952138855 27.929790259559116 28.25547758947011 23.172219198831908 14-15 19.929428819693797 30.69211400286822 27.54149871161321 21.83695846582478 16-17 20.93025515436269 28.027627408251547 28.499957452991616 22.54215998439415 18-19 20.394929440470026 28.732274371070865 27.27060055778056 23.602195630678548 20-21 20.79885573246143 28.1120759966928 27.29412956603263 23.79493870481314 22-23 20.09306470714936 29.98463833971041 27.317581682100872 22.604715271039353 24-25 19.32299647628752 30.083990031417677 27.893732485475063 22.69928100681973 26-27 19.54911722310964 30.925774117622716 27.940203255637496 21.584905403630145 28-29 19.675160644210884 29.683292065537103 27.716794833175 22.92475245707701 30-31 19.564366084936385 29.287220711209383 28.551400814301157 22.597012389553072 32-33 19.77426694879898 28.482188312991923 29.71201721908279 22.03152751912631 34-35 19.60304013986362 30.67698488318306 26.778292525641678 22.94168245131164 36-37 19.29633296186724 30.36328228016335 27.57216268248196 22.768222075487447 38-39 19.182412468533734 29.259317899587334 28.17880655437316 23.379463077505775 40-41 19.31300110082221 30.111648965682885 27.255014864501753 23.320335068993153 42-43 18.80230505133813 29.273290335520784 29.107983736691995 22.816420876449087 44-45 18.724871783753173 28.947346285375986 28.318814232345336 24.008967698525506 46-47 18.917306324200375 30.234134835710297 28.390514244673167 22.458044595416162 48-49 19.069599449507884 29.124790328995093 28.314223217916457 23.491387003580567 50-51 18.723710445845114 29.532647442626548 29.123126661744948 22.620515449783394 52-53 20.6150368117835 29.219286162790166 27.778447801251694 22.387229224174643 54-55 19.32937619747926 29.251065935373845 28.163318812050246 23.25623905509665 56-57 20.57580226572389 29.391829617720227 27.519800728998533 22.512567387557347 58-59 18.81665445556652 31.055893254635503 28.10987408415584 22.017578205642135 60-61 18.97380110872005 30.908931320740223 27.41447049385939 22.702797076680334 62-63 19.64934138645892 30.487654331055975 28.14220674623637 21.720797536248735 64-65 20.419332223538348 29.741137103983117 27.143823987173825 22.69570668530471 66-67 21.09923731997507 29.68345596044566 27.130194230869787 22.087112488709483 68-69 19.17320200739532 30.21497610898884 27.247081958451712 23.364739925164123 70-71 18.857249974864118 31.617232764794434 26.883997582761133 22.64151967758032 72-73 18.633346766709785 30.76171205761462 27.996461417956382 22.60847975771921 74-75 19.123583103865744 29.50094423236253 28.805138514287826 22.5703341494839 76-77 18.390077194799275 30.37577891851313 27.861968666039893 23.3721752206477 78-79 19.71094536460659 31.494689450023373 27.419393664643593 21.374971520726444 80-81 18.55014478070622 31.199395199483277 26.971106538421118 23.279353481389382 82-83 18.91294500963128 31.230024156316716 26.678720009044664 23.17831082500734 84-85 18.545258851960867 30.87633411125395 27.22035422314561 23.358052813639578 86-87 19.045992193042 31.395892888782306 27.493147870935125 22.064967047240568 88-89 18.308257629144602 30.992614516105654 28.1463689556054 22.552758899144344 90-91 19.015135967180584 32.28232269936932 27.50548754750315 21.197053785946952 92-93 19.55569406483665 30.901349664606066 28.403880665890746 21.139075604666534 94-95 18.438147507594405 30.678772472127903 28.83200224767014 22.05107777260755 96-97 18.666462300787547 31.13973890635388 28.895211480931426 21.29858731192715 98-99 19.544922547171765 30.900256750865445 27.04263965706901 22.51218104489377 100-101 19.443294313853045 32.10134395683337 26.47449377385051 21.98086795546308 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 45.0 1 37.5 2 32.0 3 33.5 4 30.0 5 38.5 6 47.0 7 54.0 8 66.0 9 69.0 10 76.0 11 109.5 12 159.5 13 242.0 14 371.0 15 585.5 16 926.0 17 1536.0 18 2757.5 19 4836.5 20 10846.0 21 18343.0 22 27887.5 23 59357.0 24 98625.0 25 109619.0 26 119492.5 27 174991.5 28 258849.5 29 330493.0 30 374106.5 31 409929.5 32 475747.5 33 571442.0 34 661406.0 35 801296.0 36 927365.0 37 1056539.5 38 1185102.5 39 1149258.5 40 1120640.5 41 1091646.5 42 892689.5 43 732919.5 44 702858.0 45 667152.0 46 637301.0 47 624208.5 48 641974.5 49 656174.5 50 587150.0 51 500715.0 52 470429.5 53 444920.5 54 420211.0 55 400980.5 56 358910.5 57 299709.0 58 247413.5 59 227778.0 60 204979.5 61 156455.0 62 111406.5 63 84807.0 64 71925.5 65 58187.0 66 38768.0 67 24779.0 68 13366.5 69 8054.5 70 10681.0 71 18658.0 72 32748.0 73 39233.0 74 19658.0 75 2342.0 76 1642.0 77 1428.0 78 435.5 79 220.5 80 118.0 81 71.0 82 48.0 83 36.5 84 24.0 85 13.5 86 11.5 87 6.5 88 1.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.037910176694510335 2 6.524185294691391E-5 3 0.0 4 8.714447500766359E-4 5 0.031180945576271504 6 0.049169056460206334 7 6.151374706423312E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.1650330883377484E-4 28-29 26.1071169634503 30-31 0.1870087812737987 32-33 0.05675808199763843 34-35 0.0 36-37 0.0018687130736937487 38-39 5.615459485787947E-4 40-41 5.219348235753113E-4 42-43 3.2620926473456956E-5 44-45 2.166961544308212E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 6.990198530026491E-6 58-59 0.0 60-61 0.0 62-63 0.0013188174559983312 64-65 0.0 66-67 0.001584445000139338 68-69 0.004014704022411881 70-71 0.0011673631545144239 72-73 0.00405664521359204 74-75 0.00454828917687057 76-77 0.010184719258248598 78-79 0.010366464420029286 80-81 0.0014120201030653511 82-83 0.0031199586105684902 84-85 0.009592882449373022 86-87 0.001509882882485722 88-89 0.007116022103566968 90-91 0.01183440611133485 92-93 0.014802910420419433 94-95 0.01636871489114537 96-97 0.01693492097207751 98-99 3.4018966179462254E-4 100-101 0.049259929041096676 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.1458618E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.262237882344152 #Duplication Level Percentage of deduplicated Percentage of total 1 70.36644212488534 16.36880915643285 2 10.986528046566542 5.111424578405534 3 5.044556086369209 3.520429910758428 4 2.873919409232135 2.6741518780897557 5 1.9170294796927803 2.229719789203995 6 1.3531962319185 1.888702358902794 7 1.0032572506572492 1.6336606175382855 8 0.7962892225342975 1.4818775454191766 9 0.6253063473981021 1.3091422502262944 >10 4.29478043366453 19.44606534164592 >50 0.42667379404223327 6.864415291369608 >100 0.258331552209504 11.573342997895411 >500 0.02746874630118305 4.481871968918214 >1k 0.02233131384879006 10.612620425829181 >5k 0.0026221216434423704 4.323191465563543 >10k+ 0.0012678390363897174 6.480574423801098 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC 99165 0.46212202482005127 No Hit CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT 43025 0.2005021945029265 No Hit CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 42338 0.1973006835761744 No Hit CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA 34604 0.1612592199553578 No Hit CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT 34390 0.16026195163174067 No Hit CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA 33502 0.156123754101965 No Hit CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT 31786 0.1481269669836147 No Hit CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC 31258 0.14566641710104536 No Hit CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC 29463 0.13730147952678032 No Hit CTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA 29426 0.13712905462970634 No Hit CTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCA 29018 0.13522772062953914 No Hit CTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGA 24930 0.11617709956904029 No Hit CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 24028 0.11197366018631767 No Hit ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG 23839 0.11109289517153434 No Hit CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 22756 0.10604597183285522 No Hit CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT 22353 0.10416793849445476 No Hit CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA 22014 0.10258815362666877 No Hit CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA 21849 0.10181923178836587 No Hit GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC 21671 0.1009897282294694 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.3980397060052983E-4 0.0 0.0 4.660132353350994E-6 0.0 2 1.4446410295388082E-4 0.0 0.0 4.660132353350994E-6 0.0 3 1.4446410295388082E-4 0.0 0.0 9.320264706701988E-6 0.0 4 1.4446410295388082E-4 0.0 0.0 1.3980397060052982E-5 0.0 5 1.4446410295388082E-4 0.0 0.0 1.3980397060052982E-5 0.0 6 1.491242353072318E-4 0.0 0.0 1.3980397060052982E-5 0.0 7 1.491242353072318E-4 0.0 0.0 1.3980397060052982E-5 0.0 8 1.491242353072318E-4 0.0 0.0 1.3980397060052982E-5 0.0 9 1.491242353072318E-4 0.0 0.0 1.8640529413403976E-5 0.0 10-11 1.537843676605828E-4 0.0 0.0 1.8640529413403976E-5 0.0 12-13 1.5844450001393378E-4 0.0 0.0 1.8640529413403976E-5 0.0 14-15 1.607745661906093E-4 0.0 0.0 2.0970595590079474E-5 0.0 16-17 1.7009483089731127E-4 0.0 0.0 2.330066176675497E-5 0.0 18-19 1.7475496325066229E-4 0.0 0.0 2.330066176675497E-5 0.0 20-21 1.8640529413403977E-4 0.0 0.0 2.330066176675497E-5 0.0 22-23 2.143660882541457E-4 0.0 0.0 2.330066176675497E-5 0.0 24-25 2.6329747796433116E-4 0.0 0.0 2.5630727943430466E-5 0.0 26-27 3.1688900002786757E-4 0.0 0.0 3.029086029678146E-5 0.0 28-29 4.333923088616424E-4 0.0 0.0 3.9611125003483446E-5 0.0 30-31 9.599872647903047E-4 0.0 0.0 4.194119118015895E-5 0.0 32-33 0.002104049757537974 0.0 0.0 4.194119118015895E-5 0.0 34-35 0.0039261615076982125 0.0 0.0 5.126145588686093E-5 0.0 36-37 0.006601077478521683 0.0 0.0 7.68921838302914E-5 0.0 38-39 0.010911699905371353 0.0 0.0 8.38823823603179E-5 0.0 40-41 0.01667861369264321 0.0 0.0 8.85425147136689E-5 0.0 42-43 0.023258720575574814 0.0 0.0 1.0485297795039737E-4 0.0 44-45 0.031991808605754575 0.0 0.0 1.1184317648042385E-4 0.0 46-47 0.04212992654046967 0.0 0.0 1.1650330883377484E-4 0.0 48-49 0.053887440467974224 0.0 0.0 1.1650330883377484E-4 0.0 50-51 0.06689153980000016 0.0 0.0 1.1650330883377484E-4 0.0 52-53 0.08325792462496887 0.0 0.0 1.1650330883377484E-4 0.0 54-55 0.10456870987684296 0.0 0.0 1.1650330883377484E-4 0.0 56-57 0.12825849269510273 0.0 0.0 1.1650330883377484E-4 0.0 58-59 0.15438086460181172 0.0 0.0 1.1883337501045034E-4 0.0 60-61 0.18250476335428498 0.0 0.0 1.2116344118712585E-4 0.0 62-63 0.2172786709749901 0.0 0.0 1.2116344118712585E-4 0.0 64-65 0.25728357716233174 0.0 0.0 1.2815363971715234E-4 0.0 66-67 0.3038918908943717 0.0 0.0 1.351438382471788E-4 0.0 68-69 0.35953154112720587 0.0 0.0 1.351438382471788E-4 0.0 70-71 0.4206235462134607 0.0 0.0 1.3747390442385432E-4 0.0 72-73 0.4926435616683237 0.0 0.0 1.3980397060052983E-4 0.0 74-75 0.5880108402134752 0.0 0.0 1.4446410295388082E-4 0.0 76-77 0.6951123320243643 0.0 0.0 1.9572555884074175E-4 0.0 78-79 0.8143068672921994 0.0 0.0 2.0504582354744373E-4 0.0 80-81 0.9512565068263017 0.0 0.0 2.0970595590079474E-4 0.0 82-83 1.1115720499801058 0.0 0.0 2.1203602207747025E-4 0.0 84-85 1.3381872961250347 0.0 0.0 2.1436608825414573E-4 0.0 86-87 1.571620315902916 0.0 0.0 2.1436608825414573E-4 0.0 88-89 1.8386505598822813 0.0 0.0 2.1436608825414573E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCGAAA 16425 0.0 53.832005 1 CCCGATC 810 0.0 46.28266 1 CGACCGT 150 0.0 42.670353 1 CCAAACG 21535 0.0 41.029385 8 CCGAAAC 21625 0.0 40.893883 2 TACGCAT 5440 0.0 39.11523 9 TCCACTC 51005 0.0 38.57393 2 CTCCACT 53625 0.0 37.66586 1 TCGTGTT 2665 0.0 37.041622 2 CTAACGC 1600 0.0 36.84709 2 ACTCGTC 17695 0.0 36.65638 42-43 CCACTCA 55145 0.0 35.744297 3 CTAACGA 18060 0.0 35.142353 94-95 CTACGCA 6065 0.0 34.93382 8 CGCCTAT 550 0.0 34.912106 1 ACTCATG 57200 0.0 34.787224 5 TCGTCTA 18665 0.0 34.72692 44-45 CTCGTAC 4025 0.0 34.302876 1 CTCTACG 6220 0.0 34.081387 6 TCATGAG 61285 0.0 33.5216 7 >>END_MODULE