##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2032136_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29784394
Sequences flagged as poor quality	0
Sequence length	101
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16999664320852	34.0	31.0	34.0	30.0	34.0
2	32.47278403582762	34.0	31.0	34.0	30.0	34.0
3	32.547781499264346	34.0	31.0	34.0	31.0	34.0
4	35.88212964816407	37.0	35.0	37.0	35.0	37.0
5	35.94049071470113	37.0	35.0	37.0	35.0	37.0
6	35.91634743349151	37.0	36.0	37.0	35.0	37.0
7	36.012700006587345	37.0	36.0	37.0	35.0	37.0
8	36.01292294212869	37.0	36.0	37.0	35.0	37.0
9	37.81568632888754	39.0	39.0	39.0	35.0	39.0
10-11	37.75161467109252	39.0	38.0	39.0	35.0	39.0
12-13	37.691901453492726	39.0	38.0	39.0	35.0	39.0
14-15	39.02841263112488	41.0	39.0	41.0	36.0	41.0
16-17	38.80051465878406	41.0	39.0	41.0	35.0	41.0
18-19	38.589745455287755	41.0	39.0	41.0	34.5	41.0
20-21	38.31929432574656	41.0	39.0	41.0	34.0	41.0
22-23	38.082241659843746	40.5	38.0	41.0	34.0	41.0
24-25	37.935199185184025	41.0	38.5	41.0	33.0	41.0
26-27	37.696951514272875	41.0	38.0	41.0	32.5	41.0
28-29	37.47471296545432	41.0	38.0	41.0	32.0	41.0
30-31	37.316738289185935	40.5	38.0	41.0	31.5	41.0
32-33	37.18877468180148	41.0	38.0	41.0	31.0	41.0
34-35	37.051378567581395	40.0	38.0	41.0	30.5	41.0
36-37	36.94668778555642	40.0	38.0	41.0	30.0	41.0
38-39	36.87017375609523	40.0	38.0	41.0	30.0	41.0
40-41	36.81745346237362	40.0	38.0	41.0	30.0	41.0
42-43	36.70046169144821	40.0	38.0	41.0	30.0	41.0
44-45	36.608808559274365	40.0	38.0	41.0	30.0	41.0
46-47	36.533936849613255	40.0	38.0	41.0	29.5	41.0
48-49	36.442059942532325	40.0	38.0	41.0	29.0	41.0
50-51	36.32208348774865	40.0	37.0	41.0	29.0	41.0
52-53	36.240726250801	40.0	37.0	41.0	28.5	41.0
54-55	36.104113600565455	40.0	37.0	41.0	28.0	41.0
56-57	35.988162894971104	40.0	37.0	41.0	28.0	41.0
58-59	35.84157011890186	40.0	36.0	41.0	27.0	41.0
60-61	35.76568155121773	40.0	36.0	41.0	27.5	41.0
62-63	35.68891458728353	39.0	36.0	41.0	28.0	41.0
64-65	35.45216919639191	39.0	35.0	41.0	27.0	41.0
66-67	35.21904946261455	39.0	35.0	41.0	26.5	41.0
68-69	34.94963263311652	38.5	35.0	41.0	26.0	41.0
70-71	34.61463862585218	37.5	35.0	40.0	26.0	41.0
72-73	34.302167185271585	37.0	34.5	40.0	26.0	41.0
74-75	33.9033469675428	36.5	34.0	39.0	25.5	41.0
76-77	33.6520171100342	36.0	34.0	39.0	25.5	41.0
78-79	33.4973130559581	36.0	34.0	39.0	26.0	39.5
80-81	33.17875952084169	35.0	34.0	37.0	26.0	39.0
82-83	32.83344304403171	35.0	34.0	37.0	26.0	39.0
84-85	32.54553438287178	35.0	34.0	36.5	26.0	38.0
86-87	32.27437081647523	35.0	34.0	36.0	26.0	37.0
88-89	32.045796029961195	35.0	34.0	36.0	25.0	37.0
90-91	31.851439247009694	35.0	34.0	35.5	25.0	36.5
92-93	31.664228286800128	35.0	34.0	35.0	25.0	36.0
94-95	31.478396102334663	35.0	34.0	35.0	24.0	36.0
96-97	31.373574161018688	35.0	34.0	35.0	24.0	36.0
98-99	31.244367486543457	35.0	34.0	35.0	23.0	36.0
100-101	30.899172751340856	35.0	33.5	35.0	18.5	35.5
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12878560826613494
1101	2	0.13174300561682628
1101	3	0.10946346750974811
1101	4	0.20456183674092898
1101	5	0.11268304649637884
1101	6	0.13515842542214784
1101	7	0.09083768501945144
1101	8	0.10664897380653571
1101	9	0.09428842138311211
1101	10-11	0.11019238797414488
1101	12-13	0.11282776505719738
1101	14-15	0.12268373754089623
1101	16-17	0.12963309630688258
1101	18-19	0.0923213851911413
1101	20-21	0.07460234768142726
1101	22-23	0.04327471580248954
1101	24-25	0.025540106277496477
1101	26-27	-0.0015359206600749076
1101	28-29	-0.046443335496725524
1101	30-31	-0.041341280388948576
1101	32-33	-0.041682524215261196
1101	34-35	-0.04836040923788687
1101	36-37	-0.08144220881270314
1101	38-39	-0.05617205337463105
1101	40-41	-0.03786908979098769
1101	42-43	-0.0509931366639762
1101	44-45	-0.07265472936840922
1101	46-47	-0.09703547472053486
1101	48-49	-0.07804940646047953
1101	50-51	-0.06709181438519352
1101	52-53	-0.13140292834136602
1101	54-55	-0.13916239538786357
1101	56-57	-0.1456826370366926
1101	58-59	-0.10741599298460613
1101	60-61	-0.09960572755434072
1101	62-63	-0.1452034075224944
1101	64-65	-0.15481647917626162
1101	66-67	-0.18947794058479417
1101	68-69	-0.1464861026291544
1101	70-71	-0.1558057396010497
1101	72-73	-0.1198645808520098
1101	74-75	-0.18250169135711758
1101	76-77	-0.13699391484897916
1101	78-79	-0.12190584715788333
1101	80-81	-0.10134623029139789
1101	82-83	-0.08305494173226435
1101	84-85	-0.109014223833924
1101	86-87	-0.12393722753807879
1101	88-89	-0.11871821894305867
1101	90-91	-0.14670611791540367
1101	92-93	-0.11264610906820849
1101	94-95	-0.08795082705751867
1101	96-97	-0.0857496851273396
1101	98-99	-0.14191276670987563
1101	100-101	-0.122803602907819
1102	1	0.00832972164543122
1102	2	0.03884566408844137
1102	3	0.011151225837103595
1102	4	0.12354009136398503
1102	5	0.02342493056986683
1102	6	0.04379813858837878
1102	7	0.019448691707367516
1102	8	0.02309173207449078
1102	9	-0.0031000635840143786
1102	10-11	-0.010468400855152993
1102	12-13	0.009616580434787636
1102	14-15	0.04759008265218512
1102	16-17	0.043574295958400455
1102	18-19	0.020847905694267865
1102	20-21	-0.003133620639950152
1102	22-23	-0.0028724787050435907
1102	24-25	-0.010684299478803894
1102	26-27	-0.02744014445740106
1102	28-29	-0.058447275254955855
1102	30-31	-0.07496061194341053
1102	32-33	-0.06575710667911494
1102	34-35	-0.09700529101215238
1102	36-37	-0.10489007522295424
1102	38-39	-0.09337539677629536
1102	40-41	-0.0831737555051717
1102	42-43	-0.05894005879304132
1102	44-45	-0.0798297026037531
1102	46-47	-0.07584554881992034
1102	48-49	-0.08838494816151865
1102	50-51	-0.0035038492632537555
1102	52-53	-0.027310929621982893
1102	54-55	-0.08359645753987621
1102	56-57	-0.0845158429988615
1102	58-59	-0.07945556065339332
1102	60-61	-0.08362851681748396
1102	62-63	-0.08055563940690291
1102	64-65	-0.09738146377100776
1102	66-67	-0.12304853843333774
1102	68-69	-0.12932022905854268
1102	70-71	-0.13185504112840363
1102	72-73	-0.11158607125707931
1102	74-75	-0.13431212176793395
1102	76-77	-0.11269573772821673
1102	78-79	-0.09968422296594781
1102	80-81	-0.13682473252556804
1102	82-83	-0.11844917067787009
1102	84-85	-0.11162698310915431
1102	86-87	-0.14173373893479635
1102	88-89	-0.09211998387706899
1102	90-91	-0.11130912852216923
1102	92-93	-0.11971261488009333
1102	94-95	-0.1089464947602714
1102	96-97	-0.13878289747254868
1102	98-99	-0.1265214447741343
1102	100-101	-0.09003730729557802
1103	1	-0.06212692796410835
1103	2	0.02734710303532495
1103	3	0.004457896391883764
1103	4	0.1308527836650839
1103	5	0.03493471329122144
1103	6	0.03707940391848297
1103	7	0.014010641778483546
1103	8	0.00847537749076821
1103	9	-0.009870382102739939
1103	10-11	-0.03148787910918571
1103	12-13	0.015273738704316031
1103	14-15	0.024099087024410437
1103	16-17	0.029862437600677083
1103	18-19	-0.021039200832461802
1103	20-21	-0.06905814480682437
1103	22-23	-0.06182995402100033
1103	24-25	-0.12118445195572036
1103	26-27	-0.10332086353577097
1103	28-29	-0.12511391152301599
1103	30-31	-0.09351488593404866
1103	32-33	-0.061075483854153845
1103	34-35	-0.10337960634201693
1103	36-37	-0.1284453056816801
1103	38-39	-0.08972041092374639
1103	40-41	-0.11706609513518629
1103	42-43	-0.09837268802291277
1103	44-45	-0.13533347376048255
1103	46-47	-0.20194565512110074
1103	48-49	-0.10224653281301954
1103	50-51	-0.1048319849074133
1103	52-53	-0.16585364062655117
1103	54-55	-0.1292257989165222
1103	56-57	-0.19854003473340498
1103	58-59	-0.19714026609748458
1103	60-61	-0.22812691065247037
1103	62-63	-0.19333267179283808
1103	64-65	-0.24244287504320994
1103	66-67	-0.19063248364906826
1103	68-69	-0.16549039717097713
1103	70-71	-0.16994650029863578
1103	72-73	-0.20470844998766324
1103	74-75	-0.2400750651760717
1103	76-77	-0.2117817824788304
1103	78-79	-0.18436452962092886
1103	80-81	-0.17334116470438232
1103	82-83	-0.14385843278142119
1103	84-85	-0.13588592938776856
1103	86-87	-0.15835823739082855
1103	88-89	-0.14271282110635752
1103	90-91	-0.142613002556093
1103	92-93	-0.16192289149072536
1103	94-95	-0.14750656658122097
1103	96-97	-0.14755745098248596
1103	98-99	-0.17544865663645126
1103	100-101	-0.20856930400936946
1104	1	-0.10073729841690238
1104	2	-0.03413811202196371
1104	3	-0.05104754464723271
1104	4	0.0797965064791839
1104	5	-0.026403675772549207
1104	6	0.010840794270954746
1104	7	-0.013842390437282859
1104	8	-0.006666000783816628
1104	9	-0.055248922194572
1104	10-11	-0.05678894384062261
1104	12-13	-0.055896519458194405
1104	14-15	-0.06024937538268205
1104	16-17	-0.06924444542990926
1104	18-19	-0.07456211101590071
1104	20-21	-0.07491330873021695
1104	22-23	-0.11127970606745663
1104	24-25	-0.08384769881468657
1104	26-27	-0.06989305371593701
1104	28-29	-0.09471073965807619
1104	30-31	-0.1626464453040697
1104	32-33	-0.18292030073131116
1104	34-35	-0.1945876707076124
1104	36-37	-0.15724651532040212
1104	38-39	-0.1447486920005545
1104	40-41	-0.13035164130994303
1104	42-43	-0.17504220544495297
1104	44-45	-0.14001598438982654
1104	46-47	-0.19587524409713808
1104	48-49	-0.2147328359867373
1104	50-51	-0.11986501979325936
1104	52-53	-0.19220878580436107
1104	54-55	-0.1747995150925945
1104	56-57	-0.18525348761352944
1104	58-59	-0.19078169326665773
1104	60-61	-0.17450570170196755
1104	62-63	-0.18723669212756278
1104	64-65	-0.1725664417829762
1104	66-67	-0.19999439091331794
1104	68-69	-0.19094933731349784
1104	70-71	-0.22709638712534996
1104	72-73	-0.19273708109490428
1104	74-75	-0.17785089872255355
1104	76-77	-0.17667100165136418
1104	78-79	-0.17370073392878993
1104	80-81	-0.14849936152693033
1104	82-83	-0.13522616214220307
1104	84-85	-0.12197977952239825
1104	86-87	-0.1368042783774115
1104	88-89	-0.15481725562852944
1104	90-91	-0.1696877700300483
1104	92-93	-0.16124594837538808
1104	94-95	-0.1796597401738822
1104	96-97	-0.18804898804922132
1104	98-99	-0.1817206691500175
1104	100-101	-0.18073136658004785
1105	1	-0.06744917009607576
1105	2	-0.05861484518766247
1105	3	-0.03947378707513849
1105	4	0.09025185391106305
1105	5	0.0017345179074013117
1105	6	-8.578114178163787E-4
1105	7	-0.011495507741834388
1105	8	-0.0058563193050602536
1105	9	-0.02439495084140475
1105	10-11	-0.04100560118030927
1105	12-13	-0.030281751356902475
1105	14-15	-0.09328894888417949
1105	16-17	-0.042229827102204354
1105	18-19	-0.10620758902454241
1105	20-21	-0.1109220814126175
1105	22-23	-0.09361756833094148
1105	24-25	-0.11640458212324489
1105	26-27	-0.10262191425159983
1105	28-29	-0.13661556549635634
1105	30-31	-0.14435953265189738
1105	32-33	-0.15037821065021006
1105	34-35	-0.20505054645484222
1105	36-37	-0.1978551237997408
1105	38-39	-0.1775410051214763
1105	40-41	-0.1974252609590792
1105	42-43	-0.19517397680212412
1105	44-45	-0.19629093682924292
1105	46-47	-0.1845111354321176
1105	48-49	-0.1770961786249785
1105	50-51	-0.1526036488260729
1105	52-53	-0.15361716377325507
1105	54-55	-0.19372447818353322
1105	56-57	-0.19403477452447504
1105	58-59	-0.1805541670565276
1105	60-61	-0.14501851218037842
1105	62-63	-0.17213758076479024
1105	64-65	-0.184643853936997
1105	66-67	-0.18442146479235788
1105	68-69	-0.18439420159761255
1105	70-71	-0.2541662222971368
1105	72-73	-0.24085484672502844
1105	74-75	-0.21606396765723446
1105	76-77	-0.20269513601049738
1105	78-79	-0.19854469636035788
1105	80-81	-0.18325087293469267
1105	82-83	-0.16383288997260337
1105	84-85	-0.18422745803678708
1105	86-87	-0.21919384042462298
1105	88-89	-0.18836920110476285
1105	90-91	-0.22010929799158063
1105	92-93	-0.24659494612356525
1105	94-95	-0.24969601155206433
1105	96-97	-0.23888781196029285
1105	98-99	-0.2221581182065755
1105	100-101	-0.24912369320728445
1106	1	-0.10832515691797084
1106	2	-0.0754080237418151
1106	3	-0.11663900063344812
1106	4	0.05838751118822927
1106	5	-0.012736114890252281
1106	6	-0.030172840791337308
1106	7	-0.020627127100958376
1106	8	-0.0197948444965661
1106	9	-0.04361249863028149
1106	10-11	-0.047186271289866966
1106	12-13	-0.043074776004026205
1106	14-15	-0.0677075735144399
1106	16-17	-0.06332314958567764
1106	18-19	-0.08562824459627905
1106	20-21	-0.11787025107765459
1106	22-23	-0.12211847055250757
1106	24-25	-0.10424979592203698
1106	26-27	-0.1397520334098985
1106	28-29	-0.11957791416174501
1106	30-31	-0.14004838442406253
1106	32-33	-0.13941136651027364
1106	34-35	-0.10652768419840442
1106	36-37	-0.1621479498581806
1106	38-39	-0.16119511783244178
1106	40-41	-0.19636062731174775
1106	42-43	-0.14406405163574476
1106	44-45	-0.1924813941693202
1106	46-47	-0.18457247692432333
1106	48-49	-0.18086566094507361
1106	50-51	-0.1647066249125544
1106	52-53	-0.2003042190461386
1106	54-55	-0.18564324246093378
1106	56-57	-0.18309598282391448
1106	58-59	-0.20760117369984954
1106	60-61	-0.17164960944666774
1106	62-63	-0.20599675407080298
1106	64-65	-0.23127037741667067
1106	66-67	-0.22439718390029384
1106	68-69	-0.22717325426845747
1106	70-71	-0.2193001144255362
1106	72-73	-0.193900139740947
1106	74-75	-0.1994912957317041
1106	76-77	-0.20860568060303564
1106	78-79	-0.1702745511912056
1106	80-81	-0.19133006193187896
1106	82-83	-0.19606768826781718
1106	84-85	-0.18727420223684987
1106	86-87	-0.19034875437449017
1106	88-89	-0.15989764985451416
1106	90-91	-0.18248418350179563
1106	92-93	-0.15258354046332556
1106	94-95	-0.158098612560174
1106	96-97	-0.18410952347931797
1106	98-99	-0.18709626078443264
1106	100-101	-0.19707055243944538
1107	1	-0.15757637264691482
1107	2	-0.09544445140962665
1107	3	-0.14705745617293786
1107	4	0.019625139896632504
1107	5	-0.07822836048435278
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2216	14-15	-0.1743707380724686
2216	16-17	-0.14886462550722257
2216	18-19	-0.08240250788519887
2216	20-21	-0.10092323430323091
2216	22-23	-0.07223035190801852
2216	24-25	-0.08284883634836859
2216	26-27	-0.12316474984909576
2216	28-29	-0.03520726005715602
2216	30-31	-0.034852537750552415
2216	32-33	-0.04853078108960318
2216	34-35	0.006912652719336165
2216	36-37	0.01133170935236194
2216	38-39	-0.01343411418036311
2216	40-41	-0.05149572890303489
2216	42-43	-0.08332104581360511
2216	44-45	-0.07186829596294331
2216	46-47	-0.05918150086407792
2216	48-49	-0.08187797241620132
2216	50-51	-0.023971248863816186
2216	52-53	-0.02244277437258546
2216	54-55	-0.0658088581569416
2216	56-57	-0.08858439814633101
2216	58-59	-0.16648112846451824
2216	60-61	-0.22196040173452047
2216	62-63	-0.18199066552507048
2216	64-65	-0.10990581687331513
2216	66-67	-0.08948091251430412
2216	68-69	-0.0778115963553958
2216	70-71	-0.1164696732100623
2216	72-73	-0.14195448520818843
2216	74-75	-0.10959897471143165
2216	76-77	-0.07738984487169631
2216	78-79	-0.09702844973788416
2216	80-81	-0.039112767723054276
2216	82-83	-0.041561868879732344
2216	84-85	-0.02125612876961469
2216	86-87	-0.01148243853697295
2216	88-89	-0.014720747379321608
2216	90-91	-0.04084406869649726
2216	92-93	-0.016839906581246566
2216	94-95	-0.05899517661590892
2216	96-97	-0.03613259803196911
2216	98-99	-0.07577922459991626
2216	100-101	-0.035710056435910076
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	74836.0
3	23344.0
4	20458.0
5	48071.0
6	175844.0
7	160390.0
8	126443.0
9	162386.0
10	235042.0
11	241854.0
12	187999.0
13	126487.0
14	86429.0
15	69310.0
16	60106.0
17	53476.0
18	50074.0
19	48031.0
20	50670.0
21	56303.0
22	69304.0
23	88587.0
24	112348.0
25	134468.0
26	156325.0
27	181851.0
28	212074.0
29	251914.0
30	302348.0
31	374950.0
32	478557.0
33	629490.0
34	837361.0
35	1217661.0
36	2189827.0
37	5406452.0
38	1.018713E7
39	4880619.0
40	15575.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.27672810907485	17.3908837289768	16.212339788765913	39.12004837318243
2	16.84680720100969	20.509841775037035	54.10418632858548	8.539164695367793
3	13.061256753344827	21.625713396630108	47.24654865979435	18.066481190230718
4	18.151593742139926	29.326042643431116	39.37369758444189	13.148666029987066
5	18.67655357666112	33.100037776991115	37.489924932542166	10.7334837138056
6	16.768244385535777	34.6152751032907	36.36248864683731	12.25399186433621
7	12.988617897970927	16.13641077056699	57.28164747221809	13.593323859244
8	13.923254574190766	21.31284255775021	47.749415348185366	17.014487519873665
9	16.348556313768572	22.28211411158132	45.15637863674762	16.212950937902487
10-11	18.196826758579437	30.309919204764462	37.120566211957744	14.372687824698357
12-13	18.556094399701614	22.424662343494546	42.81129787981225	16.20794537699159
14-15	18.533171380102882	26.120886272220833	40.928862504635184	14.417079843041103
16-17	19.861650342858972	26.92241591783076	38.329146930184514	14.886786809125752
18-19	19.864426156338226	27.47078051795687	35.43782391779102	17.226969407913877
20-21	21.711480090916314	27.26097219738337	34.573639530153685	16.453908181546634
22-23	21.11664547991559	29.51930625693519	33.43690380259175	15.927144460557471
24-25	20.950099598658834	29.431068317653615	32.228433631128944	17.390398452558607
26-27	21.4031113619646	29.59945295029892	31.916932676154197	17.080503011582277
28-29	22.119665667100413	29.252416073659383	31.36444907402075	17.263469185219456
30-31	21.26846059460036	30.937166663624904	30.008531429999337	17.785841311775403
32-33	21.22435981892939	29.650954660099043	31.299722291169463	17.824963229802105
34-35	22.321998354572777	30.034686613274232	29.70478852902164	17.93852650313135
36-37	22.25606826979186	30.300586729623326	29.679921882663272	17.763423117921537
38-39	22.493536618468266	28.314400975739098	31.384315146868396	17.807747258924245
40-41	22.647329050654978	30.064582040912853	28.943106677888007	18.34498223054416
42-43	22.60747046233657	29.81556629132521	29.593706468265303	17.983256778072924
44-45	22.996409844754602	28.962413145840266	29.871631443080197	18.169545566324928
46-47	21.299547875601032	30.15418313680388	29.597241051946337	18.94902793564875
48-49	21.630764249498664	29.637716170639468	29.933023355170185	18.79849622469168
50-51	21.669291344401444	29.654911001406454	30.523118768125872	18.152678886066234
52-53	23.869129679131138	28.23550759746621	28.838688257099488	19.05667446630317
54-55	22.744817344262717	28.365954463712995	29.73761817662869	19.1516100153956
56-57	23.198607771132927	28.391977304158704	29.627688397089162	18.781726527619202
58-59	22.473422645834177	29.342716638043647	28.87629326492179	19.307567451200377
60-61	22.836483531216487	28.987639629972094	28.50501103525303	19.670865803558392
62-63	23.47447901220304	28.606669369272847	28.8433780382908	19.07547358023331
64-65	23.181290590930846	28.65321001729756	29.104069725095	19.06142966667659
66-67	23.55665416963662	28.328893831771357	28.25425229022448	19.860199708367546
68-69	23.909599066671912	28.000188578951285	28.77380903734224	19.316403317034556
70-71	22.77630361003837	27.786281995940527	29.42827088527243	20.009143508748675
72-73	23.463509470942277	27.443210017033266	29.174329263066024	19.91895124895843
74-75	24.418734299503438	27.646470636612854	28.858290884118652	19.076504179765056
76-77	22.73376431455531	27.947302421320757	29.217107245408208	20.101826018715727
78-79	22.431086470756227	28.348884825308478	28.890115173818167	20.329913530117132
80-81	22.821677285060908	27.82162555519748	29.771362737259523	19.585334422482095
82-83	22.8393635553006	28.68029699283476	28.456727810323596	20.02361164154104
84-85	22.509718830808296	28.535797049608735	28.730501198871917	20.223982920711055
86-87	22.305514856227145	29.59847974823887	29.275251239460548	18.820754156073438
88-89	23.618821005097807	27.14002390866741	30.212662188801893	19.02849289743289
90-91	23.56751775939055	27.455257889643153	28.932676623669522	20.04454772729677
92-93	23.330808781409385	27.52700276448766	29.355507576620465	19.78668087748249
94-95	23.940940820547286	27.970221159982025	28.373954880286767	19.71488313918392
96-97	23.383090018781512	27.643012982404585	29.343737469785726	19.630159529028177
98-99	25.295780071259514	26.742299819499195	28.69715512989204	19.264764979349255
100-101	25.68096004285777	27.204462327794076	27.79740060368502	19.31717702566313
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	41.0
1	170.0
2	361.0
3	582.5
4	933.5
5	1456.5
6	2079.5
7	2969.0
8	4075.5
9	5205.5
10	6722.5
11	8573.0
12	10800.5
13	13634.0
14	16849.0
15	20314.0
16	24459.0
17	29713.5
18	36139.0
19	44064.5
20	58287.0
21	77472.0
22	100226.0
23	132596.5
24	167161.5
25	214731.5
26	266243.0
27	365850.0
28	494132.0
29	661522.0
30	839955.0
31	874596.5
32	962445.5
33	1156201.0
34	1444052.5
35	1482035.0
36	1299220.0
37	1379360.5
38	1416040.5
39	1514477.5
40	1557579.0
41	1366362.5
42	1126582.5
43	925015.5
44	832592.5
45	745343.0
46	666499.0
47	624010.5
48	623018.5
49	621072.0
50	571488.0
51	524311.0
52	529045.5
53	518020.0
54	555196.0
55	494792.0
56	379321.5
57	394423.0
58	385493.5
59	300879.0
60	207754.0
61	144170.0
62	104394.0
63	79406.0
64	62133.5
65	47697.0
66	32924.5
67	20698.0
68	11945.5
69	7494.0
70	5003.5
71	5523.5
72	14835.0
73	30500.5
74	33342.0
75	22020.0
76	12569.0
77	26442.5
78	28109.5
79	7666.5
80	1693.0
81	467.0
82	186.0
83	112.0
84	80.5
85	62.5
86	46.0
87	40.5
88	30.5
89	20.5
90	17.5
91	13.5
92	15.0
93	18.5
94	13.0
95	10.0
96	11.0
97	13.0
98	14.0
99	15.0
100	61.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014292719872024254
2	0.016649658878404576
3	0.013936828796986772
4	0.0058856325900067
5	0.005758048997068733
6	0.006526908017668582
7	3.3574629720517397E-6
8	0.0
9	0.0030687211564552896
10-11	7.117821500749688E-4
12-13	5.657325107907181E-4
14-15	0.004398276493387779
16-17	0.005598569505896276
18-19	0.006557125184417048
20-21	0.002486201330804313
22-23	0.008114988003449054
24-25	0.012159052153285374
26-27	0.01438505010375568
28-29	0.019510217330592657
30-31	0.015140479272467321
32-33	0.01740005185265814
34-35	0.016431423785221215
36-37	0.018805150106461794
38-39	0.026831165341151476
40-41	0.02219618770823405
42-43	0.030423650721246837
44-45	0.02627718395076294
46-47	0.028227869937525
48-49	0.03803669801037416
50-51	0.04618190318057168
52-53	0.03907751153171019
54-55	0.03677093446991065
56-57	0.028681127438751987
58-59	0.03842112752067409
60-61	0.029914995080980996
62-63	0.03580398513395975
64-65	0.041204464324504975
66-67	0.056365088374804605
68-69	0.04824506417689747
70-71	0.04548355088238491
72-73	0.04051114822077629
74-75	0.04178530541866993
76-77	0.040103216469672
78-79	0.04787742198145781
80-81	0.048199738426774774
82-83	0.05665550892188709
84-85	0.05509596737136905
86-87	0.048978669836290775
88-89	0.042854657375268404
90-91	0.03595507096770208
92-93	0.05840474713032604
94-95	0.09117862193200908
96-97	0.08419342021865545
98-99	0.07639571246606527
100-101	0.21408023275544902
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.9784394E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.024103725842263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.69029366384822	11.522029267709597
2	8.440321272774792	2.5361652456320294
3	3.511958273713653	1.5829207615531158
4	2.062936606162612	1.239750942032964
5	1.4291884026344304	1.073613740247525
6	0.9880990561781328	0.8907181626856265
7	0.7831135567515399	0.8235905514023877
8	0.615943094200959	0.7403194349193153
9	0.4685787341890678	0.6335977955562383
>10	3.823575164734368	11.688649390774305
>50	0.5722327580623661	6.022680994870585
>100	0.48979232914081966	14.963825509116072
>500	0.0646570302640637	6.705272479039477
>1k	0.0488470140298515	14.481336736617465
>5k	0.005735629740138897	6.060037632613422
>10k+	0.004727413574880107	19.0354913552299
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA	203129	0.6819980960498978	No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA	180657	0.6065491881419511	No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT	165101	0.5543204941487143	No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT	138311	0.46437406112744817	No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC	111852	0.3755389483499312	No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA	109521	0.3677127021620786	No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG	105179	0.3531345979374299	No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA	86445	0.2902358866190126	No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA	75476	0.2534078752785771	No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG	74707	0.25082598625306934	No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT	73019	0.24515858875624597	No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG	69637	0.23380364898476697	No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA	69166	0.2322222839249306	No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT	65190	0.2188730111480529	No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA	64254	0.21573042580621246	No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT	63522	0.2132727629106706	No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA	62086	0.20845144608280433	No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT	61160	0.20534243537068442	No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG	60915	0.2045198569425317	No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC	59298	0.19909083931672406	No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA	57251	0.19221811261293414	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	50087	0.16816524788115547	No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG	49308	0.16554978422592717	No Hit
CAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCA	48106	0.16151411373352098	No Hit
CGCAGGATATTTTATTGAGACAAGGTGGGTCAACGGGAAGCTGAGCAGTG	47622	0.15988910165504794	No Hit
CTCAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCT	43288	0.1453378571341757	No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG	42164	0.14156406875358957	No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC	41590	0.13963688500763183	No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC	41015	0.13770634379870209	No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA	39780	0.1335598770282182	No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT	39357	0.1321396701910403	No Hit
CAAAGACCAAGAGGTACAGGTGCAAGGGAGAGAAGAAGGGCATGGCCAGA	38473	0.1291716729237466	No Hit
CGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAG	38239	0.12838602658828646	No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT	38186	0.12820808105076772	No Hit
GTGAAATAAATGCTTTTTATTTGTCAGAAGACAGATTTTCAAATGTCTTT	37856	0.12710011826999065	No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG	37840	0.12704639886243782	No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA	37439	0.1257000562106451	No Hit
CGAACGTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGA	37160	0.12476332404144264	No Hit
CAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTG	36722	0.12329275525968397	No Hit
CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA	35054	0.11769250702230168	No Hit
AATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAA	34783	0.11678263455687567	No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT	34649	0.11633273451862074	No Hit
AGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATAATAAAT	34213	0.11486888066280616	No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA	33814	0.11352925293695752	No Hit
CTCAGGCTTTATTCAAAGACCAAGAGGTACAGGTGCAAGGGAGAGAAGAA	33787	0.11343860143671212	No Hit
GTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCT	33381	0.11207547147005911	No Hit
CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT	33338	0.1119311005622609	No Hit
CATAAATTAAGTTGAGAGCGCTTATTTGTAAGGTGGCTCTATTTCTCTTG	32910	0.11049410641022275	No Hit
TTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGAT	31401	0.10542769478539668	No Hit
GATTTATTTACCTGAAATCATGTCTGTGCAATACCTGTTACCAGCACAGA	31303	0.1050986634141356	No Hit
TATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAAT	31069	0.10431301707867549	No Hit
GTTTATTTTACACTGGGCGAATTGCTTCTGTACAATAGAAAGCAGTCTGT	30970	0.10398062824444236	No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA	30160	0.10126108323708045	No Hit
CAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGT	29785	0.10000203462256106	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001171754577246057	0.0	0.0	0.0	0.0
2	0.0011818269661622123	0.0	0.0	0.0	0.0
3	0.0011818269661622123	0.0	0.0	0.0	0.0
4	0.0011818269661622123	0.0	0.0	0.0	0.0
5	0.001185184429134264	0.0	0.0	0.0	0.0
6	0.001188541892106316	0.0	0.0	3.3574629720517397E-6	0.0
7	0.001188541892106316	0.0	0.0	3.3574629720517397E-6	0.0
8	0.001188541892106316	0.0	0.0	3.3574629720517397E-6	0.0
9	0.001188541892106316	0.0	0.0	3.3574629720517397E-6	0.0
10-11	0.001188541892106316	0.0	0.0	3.3574629720517397E-6	0.0
12-13	0.0011935780865643935	0.0	0.0	3.3574629720517397E-6	0.0
14-15	0.0012019717439945227	6.7149259441034794E-6	0.0	3.3574629720517397E-6	0.0
16-17	0.0012154015958827297	2.0144777832310438E-5	0.0	3.3574629720517397E-6	0.0
18-19	0.0012271527162849107	2.3502240804362178E-5	0.0	5.0361944580776096E-6	0.0
20-21	0.0012405825681731178	2.5180972290388046E-5	0.0	6.7149259441034794E-6	0.0
22-23	0.00126408480897748	2.6859703776413918E-5	0.0	8.39365743012935E-6	0.0
24-25	0.0013497001147647993	2.6859703776413918E-5	0.0	1.1751120402181089E-5	0.0
26-27	0.0014638538558145583	2.6859703776413918E-5	0.0	1.3429851888206959E-5	0.0
28-29	0.001752595671411008	2.6859703776413918E-5	0.0	1.3429851888206959E-5	0.0
30-31	0.002390513636100838	2.6859703776413918E-5	0.0	1.3429851888206959E-5	0.0
32-33	0.0036848156118267843	2.6859703776413918E-5	0.0	1.67873148602587E-5	0.0
34-35	0.005915849756755165	2.6859703776413918E-5	0.0	1.67873148602587E-5	0.0
36-37	0.009097045922774188	2.6859703776413918E-5	0.0	1.67873148602587E-5	0.0
38-39	0.014218855686639116	3.0217166748465657E-5	0.0	1.67873148602587E-5	0.0
40-41	0.020775980871056164	3.0217166748465657E-5	0.0	1.67873148602587E-5	0.0
42-43	0.028095250150128957	3.0217166748465657E-5	0.0	1.67873148602587E-5	0.0
44-45	0.03762876625926987	3.35746297205174E-5	0.0	1.67873148602587E-5	0.0
46-47	0.048825905271062425	3.35746297205174E-5	0.0	1.67873148602587E-5	0.0
48-49	0.06168666718550661	3.35746297205174E-5	0.0	1.67873148602587E-5	0.0
50-51	0.07589209302025751	3.35746297205174E-5	0.0	1.8466046346284567E-5	0.0
52-53	0.09370679154996406	3.35746297205174E-5	0.0	2.0144777832310438E-5	0.0
54-55	0.11659629536192678	3.35746297205174E-5	0.0	2.0144777832310438E-5	0.0
56-57	0.1418595254951301	3.35746297205174E-5	0.0	2.0144777832310438E-5	0.0
58-59	0.1691976677450614	3.35746297205174E-5	0.0	2.182350931833631E-5	0.0
60-61	0.19905222849254545	3.35746297205174E-5	0.0	2.3502240804362178E-5	0.0
62-63	0.23508619984009077	3.35746297205174E-5	0.0	2.3502240804362178E-5	0.0
64-65	0.2781742008919168	3.35746297205174E-5	0.0	2.6859703776413918E-5	0.0
66-67	0.32965753810535814	3.35746297205174E-5	0.0	2.6859703776413918E-5	0.0
68-69	0.39094802466016265	3.35746297205174E-5	0.0	2.6859703776413918E-5	0.0
70-71	0.45911291665024306	3.35746297205174E-5	0.0	2.853843526243979E-5	0.0
72-73	0.538657929384093	3.35746297205174E-5	0.0	3.0217166748465657E-5	0.0
74-75	0.6425747658320663	3.35746297205174E-5	0.0	3.6932092692569133E-5	0.0
76-77	0.7602320194931614	3.35746297205174E-5	0.0	3.6932092692569133E-5	0.0
78-79	0.8917236993305957	3.35746297205174E-5	0.0	3.6932092692569133E-5	0.0
80-81	1.0436925458345736	3.35746297205174E-5	0.0	3.8610824178595005E-5	0.0
82-83	1.2209665907589056	3.35746297205174E-5	0.0	4.0289555664620877E-5	0.0
84-85	1.4612048175296097	3.35746297205174E-5	0.0	4.196828715064674E-5	0.0
86-87	1.7164962295355077	3.35746297205174E-5	0.0	4.7004481608724356E-5	0.0
88-89	2.01126469116679	3.35746297205174E-5	0.0	5.036194458077609E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	72285	0.0	88.89951	1
GCGTGTT	80160	0.0	79.5727	2
GATCGGG	1860	0.0	60.25382	1
CGTGTTT	106625	0.0	59.55799	3
GTAAACG	5345	0.0	50.9976	1
TAAACGC	5610	0.0	48.418835	2
GTGTTTT	132165	0.0	47.632576	4
CGAACGT	7530	0.0	47.61431	1
GAGCGGC	3650	0.0	47.220837	9
GTTTTTT	417360	0.0	41.019657	1
ATCGGGA	2900	0.0	38.645164	2
CGCCGGA	1880	0.0	38.024807	46-47
CCAAGAC	16825	0.0	36.551083	1
TCGAACG	3460	0.0	36.50789	2
CGCAGGA	13855	0.0	35.646126	1
CGCGTTT	1050	0.0	34.824665	1
CGGACTT	3635	0.0	34.750637	1
ATCAATA	52785	0.0	33.751637	4
GTAGATA	34725	0.0	33.614048	2
GAACGTT	10890	0.0	33.271915	2
>>END_MODULE