FastQCFastQC Report
Fri 27 May 2016
SRR2032136_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2032136_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29784394
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC1289090.4328071942642177No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT631740.21210436579639658No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA509450.17104595111117588No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT501050.1682256822146524No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA489730.16442503413028983No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC469610.15766981863052174No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC404440.13578923244166055No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT403120.1353460473293497No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT360320.12097610580896828No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT343250.11524491651567596No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA342780.11508711575598954No Hit
CTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA341000.11448948734696432No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG334630.11235078343376735No Hit
CTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGA333760.11205868415519887No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA327970.1101147130943809No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC323210.10851656071968428No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT305000.10240262064757806No Hit
CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT298670.1002773465862693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA186550.054.3616181
CCGAAAC252150.040.186042
CCCGATC13050.039.941141
CCAAACG259500.038.8068058
TACGCAT77150.038.537349
CGACCGT1950.037.5155641
TCCACTC690350.036.9762042
TCGTGTT49400.036.730942
CTACGCA82750.035.9845628
ACTCGTC213100.035.97300342-43
CTCGTAC48700.035.8641471
TCCGCTC21050.035.8261682
CTCCACT736850.035.772261
CTAACGA210150.035.37185394-95
TCGTCTA220650.034.6385944-45
CCACTCA749700.034.3415573
CGGCAAT146100.034.1429181
CGTACCA48950.034.0806543
TCGTACC49550.033.9447562
CTCTACG88100.033.8151866
Produced by FastQC (version 0.11.5)