FastQCFastQC Report
Fri 27 May 2016
SRR2032135_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2032135_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31006692
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA2921950.9423610877290619No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT2403950.7753003770927902No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA2138380.6896511243443835No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT1803200.5815518791878862No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA1684920.5434052752225229No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG1595700.5146308416260593No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC1499080.4834698264490775No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA1055450.3403942606970134No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG959630.3094912543395471No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA945930.30507285330534456No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA939970.3031506875999542No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG920650.29691977460865543No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT905080.29189827795883544No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT889190.2867735777812093No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT839500.2707480049790542No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA786100.2535259162763961No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA780820.2518230580675939No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT757580.2443279018606693No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA728030.23479770108981637No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG675780.21794650006521174No Hit
AATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAA622920.20089856731572656No Hit
CGCAGGATATTTTATTGAGACAAGGTGGGTCAACGGGAAGCTGAGCAGTG619540.19980848005327367No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG618780.19956337167473395No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA579920.18703059326676963No Hit
CGAACGTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGA566500.1827024953193975No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG565950.18252511425598061No Hit
TATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAAT558680.18018045910863373No Hit
CAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCA555450.17913874849983996No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC553230.17842277402568452No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC543080.17514928712808192No Hit
CGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAG531430.17139203369388775No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA513450.16559328547527738No Hit
GTTTATTTTACACTGGGCGAATTGCTTCTGTACAATAGAAAGCAGTCTGT497240.16036538176984505No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT492830.15894310815226595No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT488800.15764338872395675No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA472970.15253803920779424No Hit
TTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGAT456240.1471424297696768No Hit
ATAATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATT444350.14330777368962805No Hit
GTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAAT443890.14315941861840664No Hit
AGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATAATAAAT443750.14311426707499142No Hit
GTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCT437200.14100181986520843No Hit
GTGAAATAAATGCTTTTTATTTGTCAGAAGACAGATTTTCAAATGTCTTT434890.1402568193988575No Hit
TGTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAA434200.1400342867920254No Hit
GTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGAGGTTATTCCTC432620.13952471937348235No Hit
CTTCCACACCTTTATTGAGCTTGGAACTGTCCACTGGAACCTCAGGGGCT426460.13753805146321316No Hit
CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT425760.13731229374613713No Hit
GATTTATTTACCTGAAATCATGTCTGTGCAATACCTGTTACCAGCACAGA419120.13517082054415866No Hit
TTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATG399560.12886250490700524No Hit
CAAAGACCAAGAGGTACAGGTGCAAGGGAGAGAAGAAGGGCATGGCCAGA392680.12664362905917215No Hit
GTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATT390090.12580832550599078No Hit
CTCGCAGGATATTTTATTGAGACAAGGTGGGTCAACGGGAAGCTGAGCAG388620.1253342343001311No Hit
CACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTT369150.11905494465517315No Hit
CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA365580.11790358029808534No Hit
GTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGTGGGTCACCTGA363420.11720695648539353No Hit
CAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGT362950.11705537630392819No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG357500.115297691220979No Hit
GAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGT357160.11518803747268493No Hit
CTCAGGCTTTATTCAAAGACCAAGAGGTACAGGTGCAAGGGAGAGAAGAA350720.11311106647558533No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC345230.11134048095166037No Hit
GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC337930.10898615047358164No Hit
CTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGTGGGTC315780.10184253128324686No Hit
CTCAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCT312920.10092014975347902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT554200.085.292671
GCGTGTT614150.076.1233142
GAGCGGC40800.061.678429
GATCGGG24700.058.8308451
CGTGTTT841000.055.2547343
CGAACGT104150.050.9300961
TCGAACG48150.045.5640262
ATCGGGA32750.043.9347082
GTGTTTT1057850.043.3868874
GTCGAAC51350.042.909961
GTTTTTT3430700.041.384721
CGCCGGA23350.041.19370346-47
GAACGTT144100.037.040662
CCAAGAC186200.036.980171
GTAGATA417450.035.9808242
TCATCAA601400.035.89572
CGCAGGA174350.035.571471
TCGCAGG111050.035.4496842
TAGATAT432400.034.8453373
CCGGATC38400.034.51181448-49
Produced by FastQC (version 0.11.5)