FastQCFastQC Report
Fri 27 May 2016
SRR2032135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2032135_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31006692
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC1631950.5263218662603544No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT926260.29872906145550776No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA711540.2294794942975536No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA588430.18977516208436554No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC543550.17530086730954725No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC539740.17407210030660478No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC536740.17310456723342174No Hit
CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT462260.14908394613653078No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA444240.14327229747694464No Hit
CTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA414550.1336969451626765No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT412990.13319382796462131No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT408080.13161029883484507No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG394120.12710804493429997No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG335420.10817664780235182No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT330520.10659634378281951No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC320350.10331640666472902TruSeq Adapter, Index 2 (100% over 50bp)
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT317680.10245530222959612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA107900.053.5188871
TCCGCTC20150.044.4584962
CCCGATC17850.043.5447541
CCAAACG139150.040.7312858
TACGCAT76150.039.643429
CGGCAAT179650.039.321171
ACTCGTC121300.036.93588342-43
TCCACTC848100.036.9323922
CTCCACT871300.036.7224271
CCGAAAC158300.036.264572
CTACGCA84750.035.5128338
CTAACGA124100.035.4088194-95
CTCCGCT32800.035.267271
CTCTACG85200.035.128466
TCGTCTA128650.034.64814444-45
CCACTCA914600.034.5618933
CTCGTAC54450.033.672211
ACTCATG959350.033.4068155
TCATGAG1003100.032.959267
TCGTACC54550.032.9284672
Produced by FastQC (version 0.11.5)