##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2032135_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31006692
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79049348443878	34.0	31.0	34.0	31.0	34.0
2	32.92500547946231	34.0	31.0	34.0	31.0	34.0
3	32.95971801829102	34.0	31.0	34.0	31.0	34.0
4	36.36237803116824	37.0	37.0	37.0	35.0	37.0
5	36.332386118454686	37.0	37.0	37.0	35.0	37.0
6	36.24189087955594	37.0	37.0	37.0	35.0	37.0
7	36.194931661849	37.0	37.0	37.0	35.0	37.0
8	36.20229723312632	37.0	37.0	37.0	35.0	37.0
9	38.019728063864406	39.0	39.0	39.0	35.0	39.0
10-11	38.086353116933594	39.0	39.0	39.0	37.0	39.0
12-13	38.00442865688478	39.0	39.0	39.0	35.0	39.0
14-15	39.51130388240061	41.0	39.0	41.0	37.0	41.0
16-17	39.47835918775212	41.0	39.0	41.0	36.0	41.0
18-19	39.45242667292596	41.0	39.5	41.0	36.5	41.0
20-21	39.47472582692794	41.0	39.0	41.0	37.0	41.0
22-23	39.46972222963998	41.0	39.0	41.0	37.0	41.0
24-25	39.50772829942646	41.0	39.0	41.0	37.0	41.0
26-27	39.49118677348748	41.0	39.0	41.0	37.0	41.0
28-29	29.701053533862947	21.5	20.5	41.0	19.5	41.0
30-31	30.747225373154933	26.5	23.0	41.0	21.0	41.0
32-33	37.722229365841415	38.5	37.0	40.5	34.0	41.0
34-35	38.98167106958717	40.0	38.5	41.0	36.0	41.0
36-37	39.21973887120883	41.0	39.0	41.0	36.0	41.0
38-39	39.21091005128828	41.0	39.0	41.0	36.0	41.0
40-41	39.15527664157144	41.0	39.0	41.0	35.0	41.0
42-43	39.12165809238857	41.0	39.0	41.0	35.0	41.0
44-45	39.101559737491506	41.0	39.0	41.0	35.0	41.0
46-47	39.05255731569172	41.0	39.0	41.0	35.0	41.0
48-49	38.99606494623806	41.0	39.0	41.0	35.0	41.0
50-51	38.94913636707844	40.5	39.0	41.0	35.0	41.0
52-53	38.843871929324166	40.0	38.5	41.0	35.0	41.0
54-55	38.72854216438181	40.0	38.0	41.0	35.0	41.0
56-57	38.55897672025122	40.0	38.0	41.0	34.5	41.0
58-59	38.390073794392514	40.0	37.5	41.0	34.0	41.0
60-61	38.154653421267895	40.0	37.0	41.0	34.0	41.0
62-63	37.84959687089484	39.0	36.5	41.0	34.0	41.0
64-65	37.489612419796345	39.0	36.0	41.0	33.5	41.0
66-67	37.08886296222764	38.5	35.5	40.5	33.0	41.0
68-69	36.6405885058619	37.0	35.0	40.0	32.5	41.0
70-71	36.29711128165494	37.0	35.0	39.0	32.0	41.0
72-73	35.9611846210489	36.5	35.0	39.0	32.0	41.0
74-75	35.54207991616778	36.0	35.0	38.5	32.0	40.5
76-77	35.19211275424028	35.5	35.0	37.0	32.0	39.5
78-79	34.834458525920795	35.0	35.0	37.0	31.5	39.0
80-81	34.48855645420028	35.0	34.0	36.5	31.0	39.0
82-83	34.21813012171695	35.0	34.0	36.0	31.0	37.5
84-85	33.98674074938403	35.0	34.0	36.0	31.0	37.0
86-87	33.763911287279534	35.0	34.0	35.5	31.0	37.0
88-89	33.62290859663456	35.0	34.0	35.0	31.0	36.0
90-91	33.56216990190376	35.0	34.0	35.0	31.0	36.0
92-93	33.55823007497865	35.0	34.0	35.0	31.0	36.0
94-95	33.44718590103066	35.0	34.0	35.0	31.0	36.0
96-97	33.367773511601946	35.0	34.0	35.0	31.0	36.0
98-99	33.23448850977073	35.0	34.0	35.0	31.0	36.0
100-101	32.968093613468994	35.0	34.0	35.0	30.5	35.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.09233489371294468
1101	2	0.09289502647484227
1101	3	0.09498963974783692
1101	4	0.0739224458636869
1101	5	0.06270368565301254
1101	6	0.0792224345186554
1101	7	0.07046813313132105
1101	8	0.01918683209650851
1101	9	0.06857036474251288
1101	10-11	0.05410853851017805
1101	12-13	0.062005449209095787
1101	14-15	0.13237724718198507
1101	16-17	0.05319923719292774
1101	18-19	0.12396368326710672
1101	20-21	0.104944077559054
1101	22-23	0.136652844519773
1101	24-25	0.1296801891642687
1101	26-27	0.11591939708972632
1101	28-29	9.462463333663528
1101	30-31	8.257985600406219
1101	32-33	1.4789538086384866
1101	34-35	0.3599950157611005
1101	36-37	0.15699921527356508
1101	38-39	0.1701384818383005
1101	40-41	0.11711492166978132
1101	42-43	0.12260130421995541
1101	44-45	0.08900238146944162
1101	46-47	0.09998648313855085
1101	48-49	0.09540040394615801
1101	50-51	0.1302358473524734
1101	52-53	0.10973704222147518
1101	54-55	0.09822596910645842
1101	56-57	0.11290954347563797
1101	58-59	0.1828472262699421
1101	60-61	0.15054372954786288
1101	62-63	0.20226578284160723
1101	64-65	0.27123364147302453
1101	66-67	0.2690269461510155
1101	68-69	0.30206610720888705
1101	70-71	0.3081686187881587
1101	72-73	0.288251720123462
1101	74-75	0.2847941655635111
1101	76-77	0.32749687535507377
1101	78-79	0.3665843681872829
1101	80-81	0.3613048263310503
1101	82-83	0.296401204170202
1101	84-85	0.2678748933481856
1101	86-87	0.27851574731877093
1101	88-89	0.2506091555236125
1101	90-91	0.21472273648232232
1101	92-93	0.1770933674135975
1101	94-95	0.16146236738340747
1101	96-97	0.1208391717319941
1101	98-99	0.14286805470758424
1101	100-101	0.09525941500675827
1102	1	0.011095645940265797
1102	2	0.044816297817710904
1102	3	0.04682613168577632
1102	4	0.041866809191823506
1102	5	0.03268861364993825
1102	6	0.053755591289217364
1102	7	0.04801740705157442
1102	8	0.05541178216854803
1102	9	0.044297668691989145
1102	10-11	0.04963992385940941
1102	12-13	0.06556518878247175
1102	14-15	0.07774771518339207
1102	16-17	0.06688779803893397
1102	18-19	0.1307799901409794
1102	20-21	0.11696615547560896
1102	22-23	0.11238223045414486
1102	24-25	0.08791975512303196
1102	26-27	0.08184285703471517
1102	28-29	9.417933938574286
1102	30-31	8.259001988204151
1102	32-33	1.463953722223863
1102	34-35	0.3321148585938758
1102	36-37	0.12301418800760189
1102	38-39	0.09186313111496247
1102	40-41	0.07101824263851597
1102	42-43	0.07153242835796902
1102	44-45	0.05525477641342036
1102	46-47	0.06493400310850461
1102	48-49	0.08448152965864608
1102	50-51	0.040718128916346075
1102	52-53	0.046304498404900585
1102	54-55	0.0705388785630845
1102	56-57	0.08222821034063088
1102	58-59	0.13175548482507082
1102	60-61	0.11902562071752243
1102	62-63	0.17835250596804286
1102	64-65	0.214894027331745
1102	66-67	0.24516236225043997
1102	68-69	0.16108450893776194
1102	70-71	0.2241231816280589
1102	72-73	0.22510998305908458
1102	74-75	0.24654063374801183
1102	76-77	0.24883056068917853
1102	78-79	0.2382488446711477
1102	80-81	0.2219441987994415
1102	82-83	0.21255192525905642
1102	84-85	0.16627544102484393
1102	86-87	0.15064546855755623
1102	88-89	0.10655531664816209
1102	90-91	0.09469698198618204
1102	92-93	0.08709675374979042
1102	94-95	0.05547202066071577
1102	96-97	0.049489293944020574
1102	98-99	0.053938651807719395
1102	100-101	0.004041365465461411
1103	1	0.02252718493893724
1103	2	0.0277943514504031
1103	3	0.037744137662748756
1103	4	0.02757721843349259
1103	5	0.015970348364945153
1103	6	0.05651724425318605
1103	7	0.039866325498756794
1103	8	0.013898645601194914
1103	9	0.02249107384609772
1103	10-11	9.48339944997656E-4
1103	12-13	0.022613879904227474
1103	14-15	0.056050091267330515
1103	16-17	0.05102457158072582
1103	18-19	0.09077055919951249
1103	20-21	0.05398237767545311
1103	22-23	0.043641790776888456
1103	24-25	0.0358797909574875
1103	26-27	0.004222154688605428
1103	28-29	9.388808377397716
1103	30-31	8.198049936217448
1103	32-33	1.362226708376724
1103	34-35	0.28739069592576527
1103	36-37	0.05319148532798579
1103	38-39	0.03706385067199136
1103	40-41	0.03138036302311065
1103	42-43	0.043811523893687365
1103	44-45	0.044249296974314234
1103	46-47	0.037214561404660174
1103	48-49	0.02658981183395781
1103	50-51	0.020382554182198476
1103	52-53	0.011706907848207493
1103	54-55	0.021026763791667236
1103	56-57	0.07350201541649426
1103	58-59	0.08596896907064888
1103	60-61	0.04412589501696118
1103	62-63	0.1280674549166676
1103	64-65	0.15177532289985862
1103	66-67	0.17808810530246433
1103	68-69	0.20751373629533987
1103	70-71	0.2284357583866523
1103	72-73	0.21286670018148612
1103	74-75	0.23975242080392434
1103	76-77	0.22686181500885283
1103	78-79	0.1932802338777293
1103	80-81	0.16932320021950886
1103	82-83	0.18335779576361944
1103	84-85	0.11107787651541656
1103	86-87	0.13136770385045082
1103	88-89	0.1349331306235726
1103	90-91	0.11794252182151155
1103	92-93	0.06767794420164819
1103	94-95	0.05204611265271808
1103	96-97	0.028090818466431244
1103	98-99	0.01649574875661841
1103	100-101	-0.06693408981636395
1104	1	-0.006528283713144845
1104	2	-0.0034829244419185557
1104	3	-0.020985597681075774
1104	4	0.010024665875803862
1104	5	0.004815036396124128
1104	6	0.03559263399370849
1104	7	0.028654374169803987
1104	8	-3.25750741808406E-4
1104	9	-0.008305461323608654
1104	10-11	-0.009762243981185748
1104	12-13	-0.009260286796916262
1104	14-15	-0.004268111045441003
1104	16-17	0.006871242130927158
1104	18-19	0.05653779524877223
1104	20-21	0.020754763722166558
1104	22-23	0.01196066679696628
1104	24-25	0.036563932318287584
1104	26-27	0.014029760008810399
1104	28-29	9.378726810613152
1104	30-31	8.206045248733144
1104	32-33	1.3447822463580295
1104	34-35	0.24037472602839216
1104	36-37	0.016251357219481122
1104	38-39	0.034277680975328906
1104	40-41	-0.03539536981320168
1104	42-43	-0.02407965514783683
1104	44-45	0.014212770177465472
1104	46-47	0.014781585873649306
1104	48-49	-0.008261348136841207
1104	50-51	-0.004409069678544597
1104	52-53	-0.022939105063947807
1104	54-55	0.034957947843139436
1104	56-57	0.06972660789492124
1104	58-59	0.07831755973531784
1104	60-61	0.08227756833420585
1104	62-63	0.11569387100513495
1104	64-65	0.13050600589376415
1104	66-67	0.1227038182190725
1104	68-69	0.17112848510886636
1104	70-71	0.18337290072201284
1104	72-73	0.14492629596733053
1104	74-75	0.1613919112217843
1104	76-77	0.18878814673852418
1104	78-79	0.18562280654534646
1104	80-81	0.19770041709805497
1104	82-83	0.16567951062764763
1104	84-85	0.1207415375326164
1104	86-87	0.11046260502143213
1104	88-89	0.056702352768887465
1104	90-91	0.024113504971055022
1104	92-93	0.025735065647616295
1104	94-95	-0.013352187495719647
1104	96-97	-0.05036471459855818
1104	98-99	-0.036802346252706286
1104	100-101	-0.12254640123891392
1105	1	0.013378135138260916
1105	2	-2.4281383920055077E-4
1105	3	-3.7270498992825196E-4
1105	4	0.020157939500023758
1105	5	0.007948152199197978
1105	6	0.03949873057337783
1105	7	-6.23630626648719E-4
1105	8	-0.005531912683764517
1105	9	-0.0076330827213269
1105	10-11	0.002082417232742273
1105	12-13	-0.0041015757904716565
1105	14-15	2.2989282358309993E-4
1105	16-17	-0.029232565859501847
1105	18-19	0.06218155521671065
1105	20-21	0.011304607337635275
1105	22-23	0.040372453484408766
1105	24-25	0.034078201319516666
1105	26-27	-0.0027599048238613477
1105	28-29	9.362403900109477
1105	30-31	8.161208647904257
1105	32-33	1.3839298543553156
1105	34-35	0.21356300303308728
1105	36-37	0.032839533870010484
1105	38-39	-0.0027050573890008422
1105	40-41	-0.04061573269774499
1105	42-43	-0.019996057443989912
1105	44-45	-0.03977720818885189
1105	46-47	-7.488272308648902E-4
1105	48-49	-0.030059178228832195
1105	50-51	-0.01806884583701418
1105	52-53	-0.04512877204040677
1105	54-55	-0.01963514240026143
1105	56-57	0.009896520039077927
1105	58-59	0.03582559222195414
1105	60-61	0.03998943821748924
1105	62-63	0.09152914532047163
1105	64-65	0.12214933436899855
1105	66-67	0.1734092040613291
1105	68-69	0.16868714242430372
1105	70-71	0.21248676472634997
1105	72-73	0.16956744462405737
1105	74-75	0.19495687914417204
1105	76-77	0.19472161844719693
1105	78-79	0.174755856942987
1105	80-81	0.12589149982113668
1105	82-83	0.11385530019833112
1105	84-85	0.1308592811784024
1105	86-87	0.0883168275073416
1105	88-89	0.10113339933030119
1105	90-91	0.038004708531723
1105	92-93	0.031314603480382175
1105	94-95	0.017301378664470235
1105	96-97	0.0026467907873382046
1105	98-99	-0.01521605065824616
1105	100-101	-0.10467242782531372
1106	1	-0.03253252765592407
1106	2	-0.02751844132724557
1106	3	-0.01747240566599828
1106	4	0.014251357188790337
1106	5	-0.01952132132890938
1106	6	-0.021153716829665825
1106	7	-0.018580415517071458
1106	8	-0.028583553607816725
1106	9	-0.00878049244916923
1106	10-11	-0.024511703291729248
1106	12-13	0.008639756686079636
1106	14-15	-0.024256843248537052
1106	16-17	-0.07981003054973712
1106	18-19	-0.015410853209672837
1106	20-21	-0.06109037507401638
1106	22-23	-0.08856116245965495
1106	24-25	-0.1017797138191412
1106	26-27	-0.11032593653840195
1106	28-29	9.268294278184811
1106	30-31	8.126644589280016
1106	32-33	1.3477135156082056
1106	34-35	0.16743933079861506
1106	36-37	-0.03577890892039903
1106	38-39	-0.09224085521932324
1106	40-41	-0.08522670507534258
1106	42-43	-0.1280893503419378
1106	44-45	-0.1139135404398317
1106	46-47	-0.08106578707315748
1106	48-49	-0.08056715987433449
1106	50-51	-0.06860123666992024
1106	52-53	-0.04784901624747562
1106	54-55	-0.0313867595210624
1106	56-57	0.026962240534714965
1106	58-59	0.023644432679077454
1106	60-61	0.02874904808179224
1106	62-63	0.012804094283822565
1106	64-65	0.0750040629084765
1106	66-67	0.1016563194861817
1106	68-69	0.09644031118456553
1106	70-71	0.14502382166223526
1106	72-73	0.12905165442604272
1106	74-75	0.11354463712088148
1106	76-77	0.13193680582842404
1106	78-79	0.12851727356144949
1106	80-81	0.15191119719588642
1106	82-83	0.11893333563258324
1106	84-85	0.10437706561661741
1106	86-87	0.08596175382891857
1106	88-89	0.05968309698988605
1106	90-91	0.06101536912124317
1106	92-93	0.021329975333991058
1106	94-95	0.003015514401482733
1106	96-97	-0.01614663530690308
1106	98-99	0.006722901101795742
1106	100-101	-0.10254804371472659
1107	1	-0.0868600656943741
1107	2	-0.062170992372024614
1107	3	-0.06682113373224041
1107	4	-0.014799180812531176
1107	5	-0.015546000536502902
1107	6	-0.007075108127907015
1107	7	-0.032859952644784585
1107	8	-0.036057075184167786
1107	9	-0.048924021898123726
1107	10-11	-0.02858142186175172
1107	12-13	-0.04142695572383559
1107	14-15	-0.053111485773222
1107	16-17	-0.07969896363503892
1107	18-19	-0.013026424944598602
1107	20-21	-0.013636069076241597
1107	22-23	-0.05779376385535784
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2216	28-29	-9.476339351046935
2216	30-31	-8.438457692684892
2216	32-33	-1.8907584275058227
2216	34-35	-0.7280131161273076
2216	36-37	-0.38846868317072136
2216	38-39	-0.36574472917232015
2216	40-41	-0.30845277429111206
2216	42-43	-0.322558624267721
2216	44-45	-0.3234624920842961
2216	46-47	-0.3131672644148722
2216	48-49	-0.3850121702804259
2216	50-51	-0.3651750552194599
2216	52-53	-0.3551457116785244
2216	54-55	-0.3685787757087482
2216	56-57	-0.39774572657090346
2216	58-59	-0.419657200300648
2216	60-61	-0.4143502860731658
2216	62-63	-0.39745034046780603
2216	64-65	-0.4365047048587698
2216	66-67	-0.45226203051863223
2216	68-69	-0.4243766653497403
2216	70-71	-0.4894891574603548
2216	72-73	-0.5114280091845345
2216	74-75	-0.5293794613066751
2216	76-77	-0.4963482236943193
2216	78-79	-0.4946586928385628
2216	80-81	-0.5015230773525161
2216	82-83	-0.4947685507422648
2216	84-85	-0.438594789384112
2216	86-87	-0.41530067967275386
2216	88-89	-0.4032515527832672
2216	90-91	-0.40329568926140524
2216	92-93	-0.43676774988713163
2216	94-95	-0.40856965950413837
2216	96-97	-0.45132159318002607
2216	98-99	-0.4307630602592596
2216	100-101	-0.5045503786376528
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1527.0
3	678.0
4	772.0
5	1159.0
6	1955.0
7	3422.0
8	5251.0
9	6246.0
10	8132.0
11	11443.0
12	13384.0
13	14001.0
14	15930.0
15	18730.0
16	21165.0
17	22390.0
18	24262.0
19	27200.0
20	31092.0
21	36859.0
22	43931.0
23	55307.0
24	70122.0
25	90895.0
26	121037.0
27	172561.0
28	203482.0
29	242354.0
30	312466.0
31	410703.0
32	551395.0
33	786139.0
34	1222708.0
35	2107641.0
36	4279492.0
37	9496171.0
38	8562296.0
39	2003466.0
40	8928.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.25781975291873	10.968713890420943	7.108812322219682	57.664654034440645
2	17.28381165877197	16.045662291018008	44.38417606735633	22.286349982853697
3	20.38737960179693	20.69057866605054	23.33280183516513	35.5892398969874
4	20.51655381849902	30.88670597832171	20.676536862005733	27.92020334117354
5	20.332851816055257	35.350341968458594	24.35598529245763	19.96082092302852
6	17.2299932836217	36.07274016779813	26.38767748670962	20.309589061870543
7	14.416460075682155	23.689888280256962	41.77954724097348	20.114104403087403
8	16.13479116056624	23.073506196662322	32.69809949413501	28.093603148636433
9	16.31076285080653	23.490074336211034	34.93931568062791	25.259847132354523
10-11	20.867321157639132	33.750822241856696	24.110819367638445	21.27103723286573
12-13	20.62308033375505	27.971806860273908	27.56056337773794	23.844549428233105
14-15	19.772072106240806	30.6561870579422	27.198989818068952	22.37275101774804
16-17	20.811463860769152	27.773040090829426	28.221412655048788	23.194083393352635
18-19	20.04002877830373	28.305583194750344	27.074489274766876	24.579898752179048
20-21	21.004094857974533	27.483439703919398	27.153114237403976	24.359351200702093
22-23	20.067922756803597	29.501433109988	26.90373097523593	23.52691315797248
24-25	19.51710456568537	29.41782857713425	27.537536090596188	23.527530766584196
26-27	19.570253162301423	30.582321363605523	27.560674443052147	22.286751031040904
28-29	19.74715936246898	29.43080613189903	27.055769354506566	23.766265151125424
30-31	19.288976065504905	28.84926602978759	27.856426601694352	24.005331303013154
32-33	19.994881704645024	28.3712361804654	28.716575096583984	22.917307018305593
34-35	19.740585354929188	29.94679664634976	26.366730768957876	23.945887229763173
36-37	18.969025868746048	30.149128637933266	26.892543522950906	23.989301970369784
38-39	18.90767045049114	28.839993196911095	27.848007757469333	24.404328595128437
40-41	19.091939211312148	29.64854124013347	26.682293054731332	24.57722649382305
42-43	18.690133460927353	28.40556153742107	28.69229331823293	24.21201168341865
44-45	18.57295128797516	28.514047728202513	27.65574055729721	25.257260426525114
46-47	18.381235250764576	30.061765376325862	28.148076873211757	23.408922499697805
48-49	18.901603563514612	29.029649470507852	27.881789195700073	24.186957770277463
50-51	18.701538687196944	29.071271130761062	28.4163141298659	23.81087605217609
52-53	20.54232196069158	29.164488104696883	27.0788431735962	23.21434676101533
54-55	19.113248198163156	29.333633849105862	27.488072574784823	24.065045377946152
56-57	20.55525659678976	29.049353749980067	27.218110323057203	23.177279330172972
58-59	18.975776261459945	30.41649363950208	27.752889602025267	22.85484049701271
60-61	18.979994705659024	30.865130662761448	27.058913604843752	23.095961026735775
62-63	20.021092175004323	29.939914924669065	27.570696915630222	22.468295984696393
64-65	20.688293675442708	29.892561579932487	26.147563564665333	23.271581179959476
66-67	21.263667168184732	29.528428585455725	26.525125487424777	22.682778758934766
68-69	18.862676813305008	30.248432301738582	26.314242884430953	24.574648000525457
70-71	18.495517312738453	31.808713155183028	26.091360879662517	23.604408652416005
72-73	18.495909242598294	31.309718057667602	26.74603310012329	23.448339599610815
74-75	19.187426941805885	29.838221908812923	27.68896324619099	23.2853879031902
76-77	18.539040391109314	30.45355257563451	27.100122160377605	23.90728487287857
78-79	19.32965036841207	31.65966653467066	26.59742880980747	22.413254287109798
80-81	18.195207706521074	31.73394934226267	25.776755632947097	24.29408731826916
82-83	18.843378397849495	31.509184632228894	25.47359427809035	24.17384269183126
84-85	18.378821141424346	30.934468311462272	26.40533945122472	24.28137109588867
86-87	18.869263818484036	31.59995151305849	26.867994979018206	22.66278968943927
88-89	18.259213255779642	30.66288106546861	27.81815193688748	23.259753741864266
90-91	19.13498133337092	31.94579688739369	26.938193362147377	21.981028417088012
92-93	19.42845980469402	31.283970814659916	27.59620272471148	21.691366655934587
94-95	18.477234413968787	31.040686837384452	28.153301183997947	22.328777564648814
96-97	18.85798364880032	31.310778111759163	28.158637700013177	21.67260053942734
98-99	19.69402696955799	30.696209441161038	26.58090511020093	23.028858479080043
100-101	19.67540765468972	32.26106808211733	25.428392888943442	22.635131374249507
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	70.0
1	73.5
2	56.0
3	36.5
4	43.5
5	58.5
6	61.0
7	58.0
8	66.0
9	79.5
10	103.5
11	132.5
12	183.5
13	280.0
14	420.0
15	607.0
16	954.0
17	1619.5
18	2910.0
19	5114.0
20	11149.5
21	18116.0
22	27020.5
23	57365.5
24	95166.5
25	107053.0
26	118166.5
27	175958.0
28	269653.0
29	362165.5
30	435200.5
31	503653.0
32	604279.0
33	751510.0
34	880465.0
35	1055624.0
36	1234337.5
37	1402992.5
38	1600097.5
39	1647553.0
40	1720911.0
41	1742446.5
42	1401562.5
43	1093345.0
44	1044224.0
45	979332.5
46	906690.0
47	894566.5
48	952021.5
49	994580.0
50	901537.5
51	793212.5
52	759938.5
53	726942.5
54	705296.5
55	686260.5
56	617303.5
57	515276.5
58	424386.5
59	399211.0
60	367874.5
61	268589.0
62	179079.0
63	137880.0
64	124014.0
65	100225.5
66	61630.0
67	38881.0
68	20601.0
69	10444.0
70	7954.0
71	9032.0
72	15496.0
73	19440.5
74	10297.5
75	1962.5
76	1294.0
77	1132.5
78	530.0
79	332.5
80	200.0
81	120.0
82	88.0
83	59.0
84	32.0
85	14.5
86	12.5
87	7.0
88	2.0
89	1.0
90	0.5
91	0.5
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.037498356806330714
2	4.19264331712651E-5
3	0.0
4	9.062559785481146E-4
5	0.030751426176000976
6	0.049302260299163805
7	5.676194029340505E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.3384207512365394E-4
28-29	25.992809874719946
30-31	0.1887834406843529
32-33	0.05759724384658641
34-35	0.0
36-37	0.0019624795834396005
38-39	6.079332809833439E-4
40-41	5.369808556165875E-4
42-43	4.5151543415208565E-5
44-45	2.4833348878364707E-4
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	1.6125551219717344E-6
58-59	0.0
60-61	0.0
62-63	0.00131100731416302
64-65	0.0
66-67	0.0016044923463618757
68-69	0.004139428998101442
70-71	0.001117500699526412
72-73	0.004221669309322001
74-75	0.004653834082010425
76-77	0.010275201237203891
78-79	0.010413880977693461
80-81	0.0013916350702616067
82-83	0.0030944932790637585
84-85	0.009615666192317452
86-87	0.0013077822039190765
88-89	0.007074279320089999
90-91	0.01173456362258831
92-93	0.014901621882140798
94-95	0.016506114228502674
96-97	0.017083408962168554
98-99	3.321863551261773E-4
100-101	0.05022464182893164
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.1006692E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.973209406910204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.76650983069446	14.842008296753242
2	10.820333899951219	4.538742574727326
3	4.838212151619708	3.0441850983293315
4	2.7917513329009753	2.3420794126781135
5	1.796847243316212	1.884282675315012
6	1.286310921890235	1.6186840996319771
7	1.01823220416674	1.4948918069994144
8	0.758973586773753	1.2734489575775725
9	0.6149467366803784	1.160766601424428
>10	4.471268793718838	18.46800249911369
>50	0.47348106428608194	6.883655282037
>100	0.29981525350760035	12.061517161984815
>500	0.03236849767767797	4.728071019418681
>1k	0.02553334601970842	10.755313814705488
>5k	0.0034236195451317446	5.143646318507363
>10k+	0.0019915172517433024	9.760704380796575
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC	163195	0.5263218662603544	No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT	92626	0.29872906145550776	No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA	71154	0.2294794942975536	No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA	58843	0.18977516208436554	No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC	54355	0.17530086730954725	No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC	53974	0.17407210030660478	No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC	53674	0.17310456723342174	No Hit
CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT	46226	0.14908394613653078	No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA	44424	0.14327229747694464	No Hit
CTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA	41455	0.1336969451626765	No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT	41299	0.13319382796462131	No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT	40808	0.13161029883484507	No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG	39412	0.12710804493429997	No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG	33542	0.10817664780235182	No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	33052	0.10659634378281951	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC	32035	0.10331640666472902	TruSeq Adapter, Index 2 (100% over 50bp)
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT	31768	0.10245530222959612	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8060617366083426E-4	0.0	0.0	3.2251102439434687E-6	0.0
2	1.8060617366083426E-4	0.0	0.0	3.2251102439434687E-6	0.0
3	1.8060617366083426E-4	0.0	0.0	3.2251102439434687E-6	0.0
4	1.8060617366083426E-4	0.0	0.0	3.2251102439434687E-6	0.0
5	1.9028150439266464E-4	0.0	0.0	6.4502204878869375E-6	0.0
6	1.9028150439266464E-4	0.0	0.0	6.4502204878869375E-6	0.0
7	1.967317248805516E-4	0.0	0.0	9.675330731830406E-6	0.0
8	1.967317248805516E-4	0.0	0.0	9.675330731830406E-6	0.0
9	1.9995683512449505E-4	0.0	0.0	9.675330731830406E-6	0.0
10-11	2.1124472097829722E-4	0.0	0.0	9.675330731830406E-6	0.0
12-13	2.1769494146618414E-4	0.0	0.0	1.2900440975773875E-5	0.0
14-15	2.2898282731998628E-4	0.0	0.0	1.6125551219717344E-5	0.0
16-17	2.531711541495623E-4	0.0	0.0	1.935066146366081E-5	0.0
18-19	2.725218156132231E-4	0.0	0.0	1.935066146366081E-5	0.0
20-21	2.821971463450535E-4	0.0	0.0	2.096321658563255E-5	0.0
22-23	3.2251102439434683E-4	0.0	0.0	2.2575771707604282E-5	0.0
24-25	3.57987237077725E-4	0.0	0.0	2.2575771707604282E-5	0.0
26-27	4.4345265854222693E-4	0.0	0.0	2.580088195154775E-5	0.0
28-29	6.353467180568633E-4	0.0	0.0	2.9025992195491217E-5	0.0
30-31	0.001409373176603296	0.0	0.0	2.9025992195491217E-5	0.0
32-33	0.003112231385405447	0.0	0.0	3.063854731746295E-5	0.0
34-35	0.005761659450805007	0.0	0.0	3.225110243943469E-5	0.0
36-37	0.009825298358173779	0.0	0.0	3.5476212683378156E-5	0.0
38-39	0.016751222607042378	0.0	0.0	3.5476212683378156E-5	0.0
40-41	0.025850871160328873	0.0	0.0	4.515154341520856E-5	0.0
42-43	0.036169611385826	0.0	0.0	5.966453951295417E-5	0.0
44-45	0.049292584968431974	0.0	0.0	6.450220487886938E-5	0.0
46-47	0.06479407735594625	0.0	0.0	7.740264585464325E-5	0.0
48-49	0.08364484673179584	0.0	0.0	8.224031122055845E-5	0.0
50-51	0.10426781418669234	0.0	0.0	8.385286634253018E-5	0.0
52-53	0.12965426947189335	0.0	0.0	8.385286634253018E-5	0.0
54-55	0.16380657440013274	0.0	0.0	8.385286634253018E-5	0.0
56-57	0.20120817789914514	3.2251102439434687E-6	0.0	8.546542146450193E-5	0.0
58-59	0.24237187249771758	3.2251102439434687E-6	0.0	9.191564195238886E-5	0.0
60-61	0.2885377130846464	3.2251102439434687E-6	0.0	9.514075219633233E-5	0.0
62-63	0.3450174562317064	3.2251102439434687E-6	0.0	1.0481608292816274E-4	0.0
64-65	0.4110306252598632	3.2251102439434687E-6	0.0	1.080411931721062E-4	0.0
66-67	0.48829781648426085	3.2251102439434687E-6	0.0	1.0965374829407793E-4	0.0
68-69	0.5829193259313183	3.2251102439434687E-6	0.0	1.1126630341604967E-4	0.0
70-71	0.6856906889648209	3.2251102439434687E-6	0.0	1.1287885853802141E-4	0.0
72-73	0.8075127782092975	3.2251102439434687E-6	0.0	1.1449141365999314E-4	0.0
74-75	0.9699841569684378	3.2251102439434687E-6	0.0	1.2416674439182356E-4	0.0
76-77	1.1516578421200172	3.2251102439434687E-6	0.0	1.838312839047777E-4	0.0
78-79	1.3508438758962096	3.2251102439434687E-6	0.0	1.9350661463660813E-4	0.0
80-81	1.5803217576386412	3.2251102439434687E-6	0.0	2.031819453684385E-4	0.0
82-83	1.8480042953308273	3.2251102439434687E-6	0.0	2.0963216585632546E-4	0.0
84-85	2.214739643945249	3.2251102439434687E-6	0.0	2.209200517101276E-4	0.0
86-87	2.601315870780411	3.2251102439434687E-6	0.0	2.2253260683209933E-4	0.0
88-89	3.044457306184097	3.2251102439434687E-6	0.0	2.2575771707604282E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGAAA	10790	0.0	53.518887	1
TCCGCTC	2015	0.0	44.458496	2
CCCGATC	1785	0.0	43.544754	1
CCAAACG	13915	0.0	40.731285	8
TACGCAT	7615	0.0	39.64342	9
CGGCAAT	17965	0.0	39.32117	1
ACTCGTC	12130	0.0	36.935883	42-43
TCCACTC	84810	0.0	36.932392	2
CTCCACT	87130	0.0	36.722427	1
CCGAAAC	15830	0.0	36.26457	2
CTACGCA	8475	0.0	35.512833	8
CTAACGA	12410	0.0	35.40881	94-95
CTCCGCT	3280	0.0	35.26727	1
CTCTACG	8520	0.0	35.12846	6
TCGTCTA	12865	0.0	34.648144	44-45
CCACTCA	91460	0.0	34.561893	3
CTCGTAC	5445	0.0	33.67221	1
ACTCATG	95935	0.0	33.406815	5
TCATGAG	100310	0.0	32.95926	7
TCGTACC	5455	0.0	32.928467	2
>>END_MODULE