FastQCFastQC Report
Fri 27 May 2016
SRR1233134_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233134_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14414651
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC1488691.032761736652521No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA530070.36773002690110224No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT513730.35639433795518183No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA452350.31381266185355444No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT412320.2860423051518902No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG402550.2792644788971998No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT399770.27733588555144345No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT352340.24443186310927678No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC343490.23829227637908126No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC333580.2314173267184894No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT329780.22878111998688003No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA325660.22592291689892455No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC324850.2253609886219236No Hit
CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT324270.2249586202260464No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC320500.22234322565284448No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA310970.21573189666541354No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG307590.21338706015150832No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG298750.20725441080744864No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG278230.1930188944567579No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA276090.1915342938236937No Hit
GTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTG271510.1883569709734908No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC256030.17761789723525045No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG242070.16793330618965385No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT237130.16450623743856163No Hit
ATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACT235260.1632089462311644No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA221180.15344110655193804No Hit
TCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCA220980.15330235882922175No Hit
CACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGG220260.15280286702744314No Hit
CTGGTATCTTTCAGAGTGCCAAGTGATTTCAGAATGCAAAGAATTCTTTG204110.14159898841810323No Hit
GGACACTTGACTGCTGGAGGAAGATTGCAAAAGATGAAGGAGCCAACGCT187210.12987480584857727No Hit
CTCAGCGTCAGTTCAGTTGCTCACAGCTTCTCACAGCGCATGTTTGACTG186280.12922962893794654No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT181650.12601761915706455No Hit
CTTTTGTATTGGTATTGTATGATGAGATCAAAAAATATGTGTAATACCCA180400.1251504458900878No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT179930.12482438874170453No Hit
AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA172230.1194826014171276No Hit
GGAAGATTGCAAAAGATGAAGGAGCCAACGCTTTCTTCAAAGGTGCTTGG164410.11405756545892093No Hit
CCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCT159730.11081086874735989No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT158350.10985350946061755No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC155470.10785554225350305No Hit
CTGAGAGGCATGGGTGGTGCTTTTGTATTGGTATTGTATGATGAGATCAA149490.10370698534428618No Hit
GTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCAT147680.10245131845370381No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC147550.10236113243393821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT1700.053.1439321
CCCGAAA60400.051.0924531
CGGCAAT65600.044.7229351
TACGCAT44700.044.2004629
CTCTACG49150.040.3959126
CTACGCA49300.039.9799428
GGCAATC77500.037.6914672
TCGTACC29750.037.678592
CTCGTAC31000.037.1195451
ACTCGTC84100.036.8272642-43
TCCACTC413850.036.1753582
CTCCACT421500.035.772361
TCCGCTC16300.035.5501822
CTAACGA84400.035.42188394-95
CTCCGCT21900.034.522481
TCTACGC65150.034.411357
TCGTCTA91300.033.8710644-45
CGTACCA33500.033.6026233
CCACTCA449600.033.436213
TAACGGC16300.033.216839
Produced by FastQC (version 0.11.5)