FastQCFastQC Report
Fri 27 May 2016
SRR1233133_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233133_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24365205
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC1318560.5411651574448071No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT1313800.5392115518831054No Hit
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA1231030.5052409778616679No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT842060.34559939060639955No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG803870.3299253997657725No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC753530.30926478968676846No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA744310.30548070496431284No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA556560.22842409903795188No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA536320.2201171711873551No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA506330.20780863530596191No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT491310.20164410683185305No Hit
TGTGGATTGGCCACAGCGAGGGTCTGCTGGAGAGGTTATTCCTCGTCGTG466400.19142051133983892No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA451180.18517389859843167No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT438660.18003542346555262No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT428690.17594352274072803No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA427830.17559056039134496No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA410660.16854362604377843No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC406110.1666762089627401No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT401790.16490318878909493No Hit
CGAACGTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGA382970.1571790592363167No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG380010.1559642120803006No Hit
GTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGAGGTTATTCCTC378900.15550864439679454No Hit
CTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTT362110.14861767015709493No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA350890.1440127427616554No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT344910.14155842316943362No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA336380.13805752916915742No Hit
GAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGT333310.13679753566612718No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG326180.13387123153693967No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT325730.1336865419355183No Hit
GTCGAACGTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGA316140.12975060131856062No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG312110.12809660333249814No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA311530.12785855895733278No Hit
CGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACG296790.12180894845744167No Hit
GTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCT294490.12086497938351021No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT288340.11834088816408482No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG277620.11394117143689127No Hit
CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA277470.11387960823641746No Hit
TTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATCAC275080.11289870124220173No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC271490.1114252886441957No Hit
GTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGTGGGTCACCTGA268060.11001754346002834No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG267430.10975897801803843No Hit
GGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGG254560.10447685541738722No Hit
GTTTATTTTACACTGGGCGAATTGCTTCTGTACAATAGAAAGCAGTCTGT253040.10385301498591946No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT245900.10092260664336704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT277900.064.679991
TCGAACG61750.055.001182
GTCGAAC67200.051.106531
GCGTGTT356750.049.0122642
GTAAACG40400.047.7366641
TAAACGC42900.044.954342
CGTGTTT407200.042.3332983
CGAACGT102450.039.3643531
TAGATAT217450.036.1964533
GTAGATA218400.036.1695022
ACTCGCA51800.033.287393
GATCGGG14000.032.572461
AGATATA248700.032.0305024
GTGTTTT546850.030.7931794
CTTACGG46800.030.754181
CGCCGGA19350.030.56251346-47
ATTGAAG470000.030.0082196
GTTTTTT1565450.029.876381
TTACGGT48600.029.5170942
GATATAG276050.028.6513755
Produced by FastQC (version 0.11.5)