FastQCFastQC Report
Fri 27 May 2016
SRR1233133_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233133_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24365205
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC1806870.7415780002671843No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA795340.3264245057654963No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT767720.31508866845158906No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC751230.30832082061283705No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG640360.2628174070359761No Hit
CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT589660.24200904527583497No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT572840.2351057583960406No Hit
GTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTG556670.22846924538496596No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG547840.22484522498374218No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG544450.22345389665303453No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT512750.21044354028624013No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG483610.19848386254086517No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC468860.1924301478276091No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA464710.19072689928116754No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC437390.17951418836820784No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA417860.17149865966652036No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA412500.16929880130292357No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG409420.16803470358652842No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT400010.1641726388101393No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC393080.16132841894825017No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT386890.1587879108753651No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA370880.1522170652781292No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT366530.15043173246438926No Hit
TCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCA362010.14857662802344573No Hit
ATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACT351160.14412355652250822No Hit
CACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGG324700.13326380795893158No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC315840.12962747491761303No Hit
CCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCT315380.12943868110282675No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT306680.12586801547534693TruSeq Adapter, Index 14 (97% over 44bp)
GTCGTATTACAATCTATAGTGAGTCGTATTACAATCTATAGTGAGTCGTA297560.12212497288654046No Hit
AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA285060.11699470618039126No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC275530.11308339084362311No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA275320.11299720236295979No Hit
GTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCAT256310.10519509275624811No Hit
GTTGCTTAGAGGGAGTGGAGGGTGAAAACGGGGCGGTGAGCATCAAGTCT251830.10335640516876422No Hit
TAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCA243710.10002378391644971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAT116300.047.1429521
CCCGAAA83500.046.1850621
GGCAATC138050.039.0853542
TACGCAT62850.038.916769
CTACGCA66600.036.796818
CCGCAAA47950.036.5935821
CTCTACG72100.034.25796
ACTCGTC111800.034.2031742-43
CTCGTAC65650.033.7534641
CGCAAAC49650.033.674072
TCGTACC64550.033.4801452
CTAACGA111150.033.28458494-95
TCGTCTA122550.031.22236844-45
CGTACCA69700.031.2107963
TCCGCTC23750.030.9984682
CGACCGT3350.029.8086031
CCGAAAC138950.029.8075831
CGAGCTA132000.029.60996612-13
TCCACTC630550.029.3324662
CCAAACG139000.029.0451497
Produced by FastQC (version 0.11.5)