FastQCFastQC Report
Fri 27 May 2016
SRR1233132_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233132_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32625056
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT1868380.5726825419088936No Hit
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC1802650.5525354500540934No Hit
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA1634350.500949331703829No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG1119410.3431135872992831No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC1073090.32891591051981645No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT984780.30184775774791006No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT966950.2963826330290437No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA941390.2885481637180945No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA867350.26585394979858423No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT851650.26104169752229694No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA777380.23827698563950359No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA739560.2266846683726765No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT719250.22045939170188705No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT695410.21315212455114255No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA692930.21239197259921944No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA680030.20843795639768403No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG623780.19119660668168661No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT618410.18955063249546605No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC595260.1824548592345711No Hit
CGAACGTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGA567710.1740104292847804No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA541530.16598592198585038No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG524200.16067405370890397No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT515540.15801965213484997No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA501750.15379283946669703No Hit
CTCAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCT486890.1492380580128353No Hit
CAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTG474750.14551699160301823No Hit
CTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTT474370.14540051670715906No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG471300.14445952215377042No Hit
CATAAATTAAGTTGAGAGCGCTTATTTGTAAGGTGGCTCTATTTCTCTTG471190.144425805736548No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA468500.14360128607901854No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA453750.13908022104237922No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC447850.1372717950277235No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG445720.13661892258514438No Hit
TGTGGATTGGCCACAGCGAGGGTCTGCTGGAGAGGTTATTCCTCGTCGTG444460.13623271635150605No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT442860.1357422957373621No Hit
GTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGAGGTTATTCCTC439570.13473386834952866No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG431880.13237678427279942No Hit
GAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGT428260.13126720763329877No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT424370.13007487251516134No Hit
GTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCT420470.12887947226818552No Hit
GTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGTGGGTCACCTGA416420.12763809508863372No Hit
GATTTATTTACCTGAAATCATGTCTGTGCAATACCTGTTACCAGCACAGA405390.12425725797987903No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC390530.1197024765260173No Hit
CAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGT385260.11808715362818074No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA372610.11420976564760534No Hit
GTTTATTTTACACTGGGCGAATTGCTTCTGTACAATAGAAAGCAGTCTGT369710.11332087828446946No Hit
TGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACA366210.11224808319102962No Hit
GTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCTTCTT363200.11132547941067135No Hit
GTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAAT359640.11023429354420113No Hit
CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA352950.10818372235131182No Hit
CGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACG347140.1064028824962017No Hit
CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT345520.10590633162438096No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT343350.10524119866644827No Hit
CAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCA341620.10471093137740516No Hit
GTCGAACGTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGA328290.10062511463581857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT352400.065.631581
GCGTGTT425250.052.924532
TCGAACG64500.051.7728272
GTAAACG61350.048.159811
GTCGAAC70700.048.1063651
CGAACGT120800.046.007421
CGTGTTT512550.043.5673333
TAAACGC71250.041.2012022
CTTACGG81800.035.8295021
GTAGATA298600.035.7930762
TAGATAT297350.035.6883
TTACGGT84600.034.6434442
AGATATA314150.034.4000664
CCAAGAC187050.034.3596761
GTTTTTT2143700.034.2684821
GATATAG321600.033.087225
CGCGTTT10850.032.3974571
GTGTTTT685350.032.0840874
ATTGAAG627150.030.9725726
TACGGTC95700.030.3771113
Produced by FastQC (version 0.11.5)