FastQCFastQC Report
Fri 27 May 2016
SRR1233132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233132_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32625056
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC3018300.9251478372941336No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT1053840.32301553750589734No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA1044570.3201741630727009No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT906750.27793055742187844No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA893510.2738723268398374No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT796610.2441712283957459No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT778750.23869690829036433No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG773310.23702947820227496No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA762510.23371913905680347No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG755890.231690023765783No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA728570.22331609177927542No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT726660.2227306521711411No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC712250.21831380151500737No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC680940.2087168831219784No Hit
CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT670890.20563642863938686No Hit
GTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTG647730.1985375902496535No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG634940.19461729046534051No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC631660.19361192820634546No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC629770.19303261885588793No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG629690.19300809782518075No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC601020.18422037344548928No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA511360.15673842828039897No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT505040.15480126685453044No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA469440.1438894081898281No Hit
ATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACT454950.13944803650298715No Hit
TCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCA446440.13683961186150914No Hit
CACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGG437620.13413616822604074No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG429850.13175456311860428No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT422030.12935763236697587No Hit
CTGGTATCTTTCAGAGTGCCAAGTGATTTCAGAATGCAAAGAATTCTTTG388460.1190679948564686No Hit
CTCAGCGTCAGTTCAGTTGCTCACAGCTTCTCACAGCGCATGTTTGACTG384430.11783274793459358No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT372330.11412394204013013No Hit
CTTTTGTATTGGTATTGTATGATGAGATCAAAAAATATGTGTAATACCCA357760.109658049322582No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC349240.1070465595522656No Hit
CTGAGAGGCATGGGTGGTGCTTTTGTATTGGTATTGTATGATGAGATCAA347550.10652855277857606No Hit
CTGCAGTATAGATTTGTGGTGCTAACTTTGTGTCCGTCTCCATCCATGAT340620.10440441849356519No Hit
GGACACTTGACTGCTGGAGGAAGATTGCAAAAGATGAAGGAGCCAACGCT339430.10403966816179563No Hit
GTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCAT337840.10355231267649011No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC334570.10255001554633346No Hit
AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA329010.10084580391218333No Hit
CTGAAATTTGTGGATCTAACCATAGCTTTATGCCCATTGTCCTAGAAATG326290.10001208886813864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA150500.052.985081
CGGCAAT161850.045.6263541
TACGCAT88600.043.7949339
TCCGCTC27400.041.6025052
CTACGCA95750.040.6734778
CTCTACG97950.039.569136
CTCGTAC65450.039.450021
TCGTACC63300.039.392582
GGCAATC192950.038.2529562
ACTCGTC204850.036.4294242-43
CGTACCA69200.036.102713
CTAACGA199250.036.0747294-95
TCCACTC851750.036.0675542
CTCCGCT41550.036.049281
TCTACGC122450.035.568817
CTCCACT870550.035.5312581
TCGTCTA217900.034.28047644-45
CGCAAAC57500.033.701652
CCACTCA927000.033.2115753
CCGAAAC246750.032.6073152
Produced by FastQC (version 0.11.5)