FastQCFastQC Report
Fri 27 May 2016
SRR1233131_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233131_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50297435
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT3072330.6108323416492313No Hit
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA2897560.5760850429052694No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC2040450.40567675071303333No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG1906290.379003422341517No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA1727380.3434330199939619No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA1697680.33752814631600997No Hit
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC1627950.32366461629703386No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT1618240.32173410035720507No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA1562100.310572497384807No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA1540460.3062700911090198No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA1380290.2744255248801455No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT1341430.26669948477491945No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT1214670.2414974043905022No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT1155480.2297294086666646No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT1076500.21402681866381457No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA1073900.21350989369537432No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA995810.19798425108556728No Hit
CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA952230.18931979334532667No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC945510.18798374111920418No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG939080.18670534590879237No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT893570.17765717078813262No Hit
CTCAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCT882010.1753588428515291No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA866140.1722036123710881No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG837030.16641604089751297No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT819130.16285721130709746No Hit
CAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTG803230.15969601630778985No Hit
GTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCT797550.15856673406904348No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC785500.15617098565761853No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG782570.15558845098164548No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT779230.15492440121449536No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG763180.15173338362085462No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA758830.150868528385195No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA731420.14541894631406152No Hit
GTTTATTTTACACTGGGCGAATTGCTTCTGTACAATAGAAAGCAGTCTGT728270.14479267183306666No Hit
GAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGT687540.13669484338515472No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT658870.13099475152162332No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA657240.1306706793298704No Hit
CGAACGTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGA643270.12789320171098187No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA636350.1265173860257486No Hit
CAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTGTGTAGAAAT621440.1235530201490394No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC619660.12319912536295341No Hit
GTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAAT612320.12173980641358749No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG610640.12140579335705687No Hit
GTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTG605580.12039977784155395No Hit
CAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCA604580.120200960546No Hit
TGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACA594360.11816904778543875No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC593600.11801794664081777No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT584670.11624250819152111No Hit
TGTGGATTGGCCACAGCGAGGGTCTGCTGGAGAGGTTATTCCTCGTCGTG578410.11499791192135345No Hit
CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT568810.11308926588403563No Hit
GATTTATTTACCTGAAATCATGTCTGTGCAATACCTGTTACCAGCACAGA568590.11304552607901378No Hit
CATAAATTAAGTTGAGAGCGCTTATTTGTAAGGTGGCTCTATTTCTCTTG534550.10627778533835772No Hit
GTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGTGGGTCACCTGA528100.10499541378203481No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG522650.10391185952126583No Hit
CAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGT519400.10326570331071555No Hit
TGTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAA516110.1026115944083431No Hit
CTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTT514800.10235114375116744No Hit
GTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGAGGTTATTCCTC509350.10126758949039846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT451350.070.690181
GCGTGTT554300.056.36922
GTAAACG108350.056.3341031
TCGAACG72750.049.8177832
TAAACGC126950.048.2669532
CGAACGT139300.046.2728161
CGTGTTT714650.043.535313
GTCGAAC84750.043.492981
GTAGATA551200.039.011642
TAGATAT579700.037.4870723
GATATAG594300.036.1606375
AGATATA612950.035.500724
CTTACGG152250.033.5076681
TTACGGT155200.032.8398322
CCAAGAC254750.031.9590451
ATTGAAG1001350.031.7940256
GTGTTTT975950.031.4904354
AAACGCT198600.031.1883263
GTTTTTT3262550.030.0023171
ACGTTTA227450.029.2377554
Produced by FastQC (version 0.11.5)