FastQCFastQC Report
Fri 27 May 2016
SRR1233131_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233131_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50297435
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC4942510.9826564714482955No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA2004370.3985034226894473No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT1875350.37285201521707817No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA1686810.3353670023133386No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT1604520.31900632706220505No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT1514540.30111674680826167No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT1346260.2676597723124449No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC1305540.25956393203748857No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA1288360.2561482508998719No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA1285800.25563927862325386No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG1244380.2474042662414097No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC1184180.23543546504906265No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1150400.22871941680525062No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC1126730.22401341141948888No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG1080190.2147604544844086No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG1004470.19970600886506437No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC925850.18407499308861378No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA843580.16771829418339126No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG835840.16617944831580378No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT804440.1599365852354101No Hit
ATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACT770700.15322848968342023No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT740070.14713871592060312No Hit
CCAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACC728030.1447449556821337No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC718070.14276473541841647No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT702970.139762594255552No Hit
CTGGTATCTTTCAGAGTGCCAAGTGATTTCAGAATGCAAAGAATTCTTTG702200.13960950493797547No Hit
TCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCA690190.13722170921837265No Hit
CACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGG684580.1361063441903151No Hit
CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT671590.13352370752106943No Hit
AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA653690.12996487793065392No Hit
GTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTG648220.12887734732397388No Hit
GGACACTTGACTGCTGGAGGAAGATTGCAAAAGATGAAGGAGCCAACGCT625160.12429262048850005No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC604770.12023873583215526No Hit
CTATATTCATAGCACTTATACTAAATAACTCTATAACCATCAACTCAATC580410.11539554651246132No Hit
CTTTTGTATTGGTATTGTATGATGAGATCAAAAAATATGTGTAATACCCA579780.11527029161626234No Hit
GGAAGATTGCAAAAGATGAAGGAGCCAACGCTTTCTTCAAAGGTGCTTGG566060.1125425183212623No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCC544340.10822420666183076No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG527480.10487214705879137No Hit
CTCGTACCAACACATGATCTAGGAGGCTGCTGACCTCCAACAGGAATTTC505620.10052600097798228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA255000.052.5145031
CGGCAAT210000.047.780421
TACGCAT178250.043.190369
CTACGCA180550.042.6401638
CTCTACG179500.042.604526
CCGCAAA125400.040.9555741
GGCAATC245900.040.602232
TCCGCTC45050.039.748642
CTCGTAC124200.039.053991
CTCCGCT59550.038.5702251
TCGTACC124500.038.265492
CGCAAAC130750.038.1074142
TCTACGC236950.036.543347
CGTACCA130950.036.380853
ACTCGTC354800.036.28253642-43
TCCACTC1365400.035.7992022
CTCCACT1406250.035.1552771
CTAACGA357300.034.93715794-95
TCGTCTA374700.034.39373444-45
TAACGGC54000.034.3007979
Produced by FastQC (version 0.11.5)