##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233130_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34297129
Sequences flagged as poor quality	0
Sequence length	101
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68554166735064	34.0	31.0	34.0	31.0	34.0
2	32.861014955508374	34.0	33.0	34.0	31.0	34.0
3	32.94260738267626	34.0	33.0	34.0	31.0	34.0
4	36.21378515385355	37.0	37.0	37.0	35.0	37.0
5	36.18749799728135	37.0	37.0	37.0	35.0	37.0
6	36.22772964465918	37.0	37.0	37.0	35.0	37.0
7	36.277008375832274	37.0	37.0	37.0	35.0	37.0
8	36.307539648581084	37.0	37.0	37.0	35.0	37.0
9	38.174910471369195	39.0	39.0	39.0	37.0	39.0
10-11	38.12283821190981	39.0	39.0	39.0	37.0	39.0
12-13	38.040263180629495	39.0	39.0	39.0	37.0	39.0
14-15	39.529437901347364	41.0	40.0	41.0	37.5	41.0
16-17	39.438930034056206	41.0	40.0	41.0	37.0	41.0
18-19	39.302617953823486	41.0	40.0	41.0	37.0	41.0
20-21	39.22589710059988	41.0	40.0	41.0	37.0	41.0
22-23	39.1196982114742	41.0	40.0	41.0	36.5	41.0
24-25	39.05895652373702	41.0	40.0	41.0	36.0	41.0
26-27	38.929542207454155	41.0	39.5	41.0	36.0	41.0
28-29	38.84053207485676	41.0	39.0	41.0	36.0	41.0
30-31	38.72622637014311	41.0	39.0	41.0	36.0	41.0
32-33	38.66189094136713	41.0	39.0	41.0	36.0	41.0
34-35	38.60688267813904	41.0	39.0	41.0	35.0	41.0
36-37	38.50407764451654	41.0	39.0	41.0	35.0	41.0
38-39	38.4025054400326	41.0	39.0	41.0	35.0	41.0
40-41	38.3645729938503	41.0	39.0	41.0	35.0	41.0
42-43	38.374967216643704	41.0	39.0	41.0	35.0	41.0
44-45	38.44498675093183	41.0	39.0	41.0	35.0	41.0
46-47	38.40501271986935	41.0	39.0	41.0	35.0	41.0
48-49	38.350029517047915	41.0	39.0	41.0	35.0	41.0
50-51	38.30097924523071	41.0	39.0	41.0	34.5	41.0
52-53	38.17993672298343	41.0	39.0	41.0	34.0	41.0
54-55	38.0543091813895	41.0	39.0	41.0	34.0	41.0
56-57	37.9622025651185	40.0	38.0	41.0	34.0	41.0
58-59	37.80289516361559	40.0	38.0	41.0	33.5	41.0
60-61	37.65974547024038	40.0	38.0	41.0	33.0	41.0
62-63	37.463476724247094	40.0	37.0	41.0	33.0	41.0
64-65	37.19752087703901	40.0	37.0	41.0	32.5	41.0
66-67	36.95618267348267	39.0	36.0	41.0	32.0	41.0
68-69	36.67016933108307	39.0	36.0	41.0	32.0	41.0
70-71	36.3110927302399	39.0	35.0	41.0	31.0	41.0
72-73	35.971455905245016	37.5	35.0	40.0	31.0	41.0
74-75	35.54334849718762	37.0	35.0	39.5	31.0	41.0
76-77	35.33684420057433	37.0	35.0	39.0	31.0	41.0
78-79	34.99930236143089	36.0	35.0	39.0	31.0	40.5
80-81	34.60753837442195	36.0	35.0	38.0	31.0	39.0
82-83	34.23425988513499	35.5	35.0	37.0	31.0	39.0
84-85	33.871268393923	35.0	35.0	37.0	31.0	39.0
86-87	33.57041539832678	35.0	35.0	36.0	30.0	37.0
88-89	33.32172163740003	35.0	34.5	36.0	30.0	37.0
90-91	33.0958215482118	35.0	34.0	36.0	30.0	36.5
92-93	32.883601379579034	35.0	34.0	35.0	30.0	36.0
94-95	32.72595695983766	35.0	34.0	35.0	29.5	36.0
96-97	32.63573920429316	35.0	34.0	35.0	29.5	36.0
98-99	32.501853726590355	35.0	34.0	35.0	29.0	36.0
100-101	31.81272543249903	34.5	33.0	35.0	27.0	35.5
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.13588978020425913
1101	2	0.08751637022341185
1101	3	0.08509696236019693
1101	4	0.04583554957662983
1101	5	0.036819863014187604
1101	6	0.10250722686450331
1101	7	0.06753272440321467
1101	8	0.10313671344133013
1101	9	0.12635667765312775
1101	10-11	0.1686851358030097
1101	12-13	0.16353634801823347
1101	14-15	0.2231963434851849
1101	16-17	0.2349486285279525
1101	18-19	0.1776462078903407
1101	20-21	0.16556854276132071
1101	22-23	0.17972367400171407
1101	24-25	0.15860329409562013
1101	26-27	0.10325907585632166
1101	28-29	0.09473702610556956
1101	30-31	0.02837168582436078
1101	32-33	0.01484763306024206
1101	34-35	0.008529057174712307
1101	36-37	-0.06788691600124253
1101	38-39	-0.06695349520408911
1101	40-41	-0.09384305757226485
1101	42-43	-0.0827297125385158
1101	44-45	-0.06987523260080764
1101	46-47	-0.07049949853691828
1101	48-49	-0.09434493454904214
1101	50-51	-0.060642185748690736
1101	52-53	-0.03586471741447639
1101	54-55	-0.0731161080288274
1101	56-57	-0.09538742997208516
1101	58-59	-0.09544076007497182
1101	60-61	-0.13530458433628922
1101	62-63	-0.13509221819364825
1101	64-65	-0.19601840479257504
1101	66-67	-0.18562114015958286
1101	68-69	-0.18435047160267004
1101	70-71	-0.19971703777027727
1101	72-73	-0.2265879314832162
1101	74-75	-0.2563277611780279
1101	76-77	-0.2372829418616007
1101	78-79	-0.2599031061906558
1101	80-81	-0.2795377187197019
1101	82-83	-0.24055117278949467
1101	84-85	-0.23772393200513164
1101	86-87	-0.2776141246424757
1101	88-89	-0.2799153323863095
1101	90-91	-0.29412747377593007
1101	92-93	-0.26708584436578064
1101	94-95	-0.26263258721319716
1101	96-97	-0.2704140416110974
1101	98-99	-0.2954841497227676
1101	100-101	-0.5387842847166375
1102	1	0.11329868741388793
1102	2	0.07513097427057858
1102	3	0.06521397543284735
1102	4	0.048302353396913134
1102	5	0.04761292272839768
1102	6	0.08154207916403777
1102	7	0.05209049788141584
1102	8	0.09026645185492299
1102	9	0.09054926331324964
1102	10-11	0.126627755028224
1102	12-13	0.14150719201666107
1102	14-15	0.16468819720915917
1102	16-17	0.19770132997697232
1102	18-19	0.10189856053627722
1102	20-21	0.0976938837701411
1102	22-23	0.09633865576049772
1102	24-25	0.04817958554667001
1102	26-27	-0.01710278064134485
1102	28-29	-0.01978696817810288
1102	30-31	-0.06471862712510301
1102	32-33	-0.06767514123622931
1102	34-35	-0.11003448760700252
1102	36-37	-0.1526105969778655
1102	38-39	-0.20614842973486702
1102	40-41	-0.2111546589280735
1102	42-43	-0.17901935709871708
1102	44-45	-0.1013766611289526
1102	46-47	-0.08457773463933904
1102	48-49	-0.1118487108445052
1102	50-51	-0.10238616359338693
1102	52-53	-0.12901998608300858
1102	54-55	-0.12338593949630194
1102	56-57	-0.15247745236182197
1102	58-59	-0.16635375949839215
1102	60-61	-0.20637795098780742
1102	62-63	-0.1991261972717595
1102	64-65	-0.25374559244797723
1102	66-67	-0.2774022976109549
1102	68-69	-0.2663750783221843
1102	70-71	-0.29109787225552
1102	72-73	-0.275570270679772
1102	74-75	-0.30242456966369247
1102	76-77	-0.33006678021234137
1102	78-79	-0.30709154508335246
1102	80-81	-0.33953063967361174
1102	82-83	-0.31976095265485327
1102	84-85	-0.3203481681266638
1102	86-87	-0.32945519146052504
1102	88-89	-0.3177066773742183
1102	90-91	-0.32634641196095515
1102	92-93	-0.2944513849757797
1102	94-95	-0.3158452531187024
1102	96-97	-0.32538706580546517
1102	98-99	-0.3561653726621046
1102	100-101	-0.6040502094577711
1103	1	0.15485081713465831
1103	2	0.11908226003471611
1103	3	0.0998843283558557
1103	4	0.06863957936213438
1103	5	0.0868922889006214
1103	6	0.11676049673329203
1103	7	0.09054197239566264
1103	8	0.08106516618295245
1103	9	0.0921497684840844
1103	10-11	0.10516390890401794
1103	12-13	0.1338357582037304
1103	14-15	0.19511119641749985
1103	16-17	0.23023748826987145
1103	18-19	0.14951143413365742
1103	20-21	0.13657577419181877
1103	22-23	0.1379197278479296
1103	24-25	0.09959456157780977
1103	26-27	0.08208938855337067
1103	28-29	0.05004767953084155
1103	30-31	0.006971899027199413
1103	32-33	0.0384312860037781
1103	34-35	-0.020429991724533636
1103	36-37	-0.03667794635335042
1103	38-39	-0.10245537727882947
1103	40-41	-0.12115465362379751
1103	42-43	-0.08695907648047552
1103	44-45	-0.04764237822034545
1103	46-47	-0.03800517473748499
1103	48-49	-0.03540192986116608
1103	50-51	-0.007018409704102169
1103	52-53	-0.015143705360081583
1103	54-55	-0.05669431607807951
1103	56-57	-0.08365320537566845
1103	58-59	-0.10514280042219326
1103	60-61	-0.17586058885978417
1103	62-63	-0.15525934465876645
1103	64-65	-0.22306504930024573
1103	66-67	-0.2672828760516168
1103	68-69	-0.26289957994079316
1103	70-71	-0.255159633843256
1103	72-73	-0.2551139487935572
1103	74-75	-0.2696721820457313
1103	76-77	-0.27190390652909713
1103	78-79	-0.25795130276083
1103	80-81	-0.2962275772282794
1103	82-83	-0.28698199852662754
1103	84-85	-0.25223502589829394
1103	86-87	-0.24757092405774017
1103	88-89	-0.29395315510326014
1103	90-91	-0.2804476576595363
1103	92-93	-0.23546026651273166
1103	94-95	-0.27625325430044256
1103	96-97	-0.2626935306235936
1103	98-99	-0.3038993690018117
1103	100-101	-0.6376274721921682
1104	1	0.12546593475646262
1104	2	0.05211171287352556
1104	3	0.07396041101509354
1104	4	0.05063509373623276
1104	5	0.054405514571747915
1104	6	0.08854179764729508
1104	7	0.07551191667496227
1104	8	0.0696600161507277
1104	9	0.1042574748436138
1104	10-11	0.11919233264286078
1104	12-13	0.12731577183326692
1104	14-15	0.15442661167026728
1104	16-17	0.1599304680066851
1104	18-19	0.09936884416644176
1104	20-21	0.10483466323249502
1104	22-23	0.11799051500651814
1104	24-25	0.08920894674871249
1104	26-27	0.0688210630106525
1104	28-29	0.03464424453348158
1104	30-31	0.01812902464711641
1104	32-33	0.007794647972808377
1104	34-35	-0.01612173305679221
1104	36-37	-0.04784430691813668
1104	38-39	-0.09385105248902903
1104	40-41	-0.1420998490447758
1104	42-43	-0.10391546330946966
1104	44-45	-0.020191993397908448
1104	46-47	-0.02668128283818305
1104	48-49	-0.04612534123127432
1104	50-51	-0.04461892983254501
1104	52-53	-0.04106205046082323
1104	54-55	-0.06984465719941113
1104	56-57	-0.10136081176069922
1104	58-59	-0.09440671406679257
1104	60-61	-0.102513530240536
1104	62-63	-0.1359270465882858
1104	64-65	-0.1724423800099828
1104	66-67	-0.16174337296168773
1104	68-69	-0.182534041821377
1104	70-71	-0.21070818940997782
1104	72-73	-0.231140682604412
1104	74-75	-0.2378668513694393
1104	76-77	-0.22509232437784732
1104	78-79	-0.19863773787453454
1104	80-81	-0.28213663377790255
1104	82-83	-0.24482844709452678
1104	84-85	-0.2440180627943036
1104	86-87	-0.22024247615890147
1104	88-89	-0.24631236288448122
1104	90-91	-0.23981858432331649
1104	92-93	-0.20042312231563386
1104	94-95	-0.2292597238247538
1104	96-97	-0.23916671392236566
1104	98-99	-0.30824995934752764
1104	100-101	-0.6113360090493423
1105	1	0.14867085122451584
1105	2	0.10901960222614804
1105	3	0.07219439391704441
1105	4	0.06234626772165086
1105	5	0.041270702422984584
1105	6	0.10970584148288509
1105	7	0.09432133915162666
1105	8	0.09322886886626236
1105	9	0.10513749151471785
1105	10-11	0.1344721096231396
1105	12-13	0.14306054793789258
1105	14-15	0.1538515781053107
1105	16-17	0.16339559663281733
1105	18-19	0.12058524810424842
1105	20-21	0.12037000055624247
1105	22-23	0.10143846707059367
1105	24-25	0.08864085095428464
1105	26-27	0.09154043810668355
1105	28-29	0.09434388322141984
1105	30-31	0.0880863866056103
1105	32-33	0.08823986115844207
1105	34-35	0.04333398027806368
1105	36-37	0.0013578109690399742
1105	38-39	-0.036874088648282566
1105	40-41	-0.09836965081182569
1105	42-43	-0.05590549094851127
1105	44-45	0.022569340256509918
1105	46-47	0.016825908766996633
1105	48-49	0.015260169996160755
1105	50-51	0.021747922609868908
1105	52-53	0.025478674411701263
1105	54-55	0.0021153529052071463
1105	56-57	-0.030298267760237252
1105	58-59	-0.053081002774533204
1105	60-61	-0.049301756653640894
1105	62-63	-0.08335242252680786
1105	64-65	-0.11321364442967052
1105	66-67	-0.11302364420948408
1105	68-69	-0.0938957884781928
1105	70-71	-0.1255803185254578
1105	72-73	-0.1463848855822718
1105	74-75	-0.15121366934743463
1105	76-77	-0.12392575051488564
1105	78-79	-0.14199990834178777
1105	80-81	-0.18192221946863185
1105	82-83	-0.15722939941085912
1105	84-85	-0.1572369158212723
1105	86-87	-0.1544123509875135
1105	88-89	-0.16215833992134066
1105	90-91	-0.18727749078435352
1105	92-93	-0.14157523313708253
1105	94-95	-0.17269629441238266
1105	96-97	-0.19518543929630283
1105	98-99	-0.2478338259292059
1105	100-101	-0.5942027134290022
1106	1	0.1308569085443665
1106	2	0.08633667481737461
1106	3	0.08455887128774009
1106	4	0.0699410167441954
1106	5	0.06746564353402107
1106	6	0.09386653369882225
1106	7	0.08524489967684445
1106	8	0.08297707707068014
1106	9	0.08231433194193016
1106	10-11	0.11715159053867552
1106	12-13	0.1280184193237588
1106	14-15	0.12647150614137104
1106	16-17	0.1486307599425274
1106	18-19	0.11224377199014413
1106	20-21	0.14099981199225198
1106	22-23	0.15126974573275476
1106	24-25	0.1384668998639924
1106	26-27	0.107651730970872
1106	28-29	0.05310102236129666
1106	30-31	0.05647263740238628
1106	32-33	0.03401132195277512
1106	34-35	-0.002423437058475031
1106	36-37	-0.010240438581789135
1106	38-39	-0.07170434177925245
1106	40-41	-0.06736576542419215
1106	42-43	-0.051835748668189296
1106	44-45	0.026821934109563017
1106	46-47	0.04410620823436773
1106	48-49	0.018166592715296304
1106	50-51	0.02310836116156878
1106	52-53	0.020080826246982042
1106	54-55	-0.022568455613182437
1106	56-57	-0.06998061077377571
1106	58-59	-0.08237251140593571
1106	60-61	-0.08666112653071423
1106	62-63	-0.10060434363408177
1106	64-65	-0.13469988441271852
1106	66-67	-0.13944554020987
1106	68-69	-0.1300240575888978
1106	70-71	-0.154467692437116
1106	72-73	-0.143400701700223
1106	74-75	-0.17658030500061983
1106	76-77	-0.20557005968178288
1106	78-79	-0.177588033588421
1106	80-81	-0.20035833605484754
1106	82-83	-0.19373662622425059
1106	84-85	-0.1730776685722688
1106	86-87	-0.192871005065129
1106	88-89	-0.16121739662169432
1106	90-91	-0.1732954272796121
1106	92-93	-0.15793935271735648
1106	94-95	-0.19527164136367503
1106	96-97	-0.165416638711811
1106	98-99	-0.2184168850742978
1106	100-101	-0.560928227741659
1107	1	0.13847782339161796
1107	2	0.10061861009688755
1107	3	0.08600115681660725
1107	4	0.06724111301684843
1107	5	0.07476413991185638
1107	6	0.10335965266443736
1107	7	0.07368199614553106
1107	8	0.04422895471996924
1107	9	0.09375652719951688
1107	10-11	0.08772593425738506
1107	12-13	0.12341321991777932
1107	14-15	0.11668800391279888
1107	16-17	0.14330600603383203
1107	18-19	0.07490063120348367
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2216	3	-0.2955203296623736
2216	4	-0.16845566208916551
2216	5	-0.30356102138618724
2216	6	-0.2088788376299462
2216	7	-0.17673037492059507
2216	8	-0.20424142745565632
2216	9	-0.32243442210309325
2216	10-11	-0.28886327010042834
2216	12-13	-0.31279532951690925
2216	14-15	-0.44607093135476106
2216	16-17	-0.5257185597837335
2216	18-19	-0.38414531502191807
2216	20-21	-0.4390574913255776
2216	22-23	-0.4164348114112002
2216	24-25	-0.4516499445686719
2216	26-27	-0.3608873333909415
2216	28-29	-0.37145810252465594
2216	30-31	-0.37080075860958317
2216	32-33	-0.36245060966894016
2216	34-35	-0.3649096951837123
2216	36-37	-0.37049521218102655
2216	38-39	-0.3444068248086154
2216	40-41	-0.3600228023594454
2216	42-43	-0.33966367478878823
2216	44-45	-0.4109382703579314
2216	46-47	-0.45283180712748106
2216	48-49	-0.39668112255642995
2216	50-51	-0.33514212038324587
2216	52-53	-0.29051880419631004
2216	54-55	-0.32524814024900195
2216	56-57	-0.31246185223096035
2216	58-59	-0.3178172024832122
2216	60-61	-0.2674358782731332
2216	62-63	-0.34244428268723226
2216	64-65	-0.30316924039667725
2216	66-67	-0.3532170687308138
2216	68-69	-0.3818270778072659
2216	70-71	-0.34647918333853767
2216	72-73	-0.31526711459159174
2216	74-75	-0.3118949078320341
2216	76-77	-0.22261440792726006
2216	78-79	-0.20663173295449866
2216	80-81	-0.1426636947914801
2216	82-83	-0.17207302141225256
2216	84-85	-0.1369482846315364
2216	86-87	-0.13133535553256337
2216	88-89	-0.1380810714249776
2216	90-91	-0.2030364889798193
2216	92-93	-0.19311004530077724
2216	94-95	-0.17414332017605716
2216	96-97	-0.1873526283805873
2216	98-99	-0.16481816998231125
2216	100-101	-0.020632131942651455
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	55760.0
3	19477.0
4	17167.0
5	25711.0
6	88093.0
7	83011.0
8	62171.0
9	77084.0
10	106080.0
11	103314.0
12	82806.0
13	55972.0
14	50980.0
15	46496.0
16	44115.0
17	43572.0
18	46106.0
19	43136.0
20	50059.0
21	62125.0
22	69189.0
23	83309.0
24	97198.0
25	111454.0
26	134850.0
27	173889.0
28	176443.0
29	196996.0
30	233133.0
31	296295.0
32	395764.0
33	564394.0
34	841705.0
35	1169126.0
36	2102277.0
37	5793314.0
38	1.3239859E7
39	7434073.0
40	20626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.000917217469688	18.114413672093875	18.702917704430906	37.181751406005525
2	15.01922530146702	21.77648537182155	54.27776901536715	8.926520311344275
3	13.681428244548712	22.432756599172077	47.261413309297176	16.624401846982035
4	17.269963153427838	31.156879502485896	37.8247169522906	13.748440391795667
5	19.65555278562418	36.14052275790196	32.493065929781615	11.710858526692245
6	15.672438929388829	37.01665924917565	33.945085750785836	13.365816070649677
7	13.384290712751648	16.069829682222476	55.97694530075881	14.568934304267067
8	14.881830896076762	22.122985429651603	43.954668290470764	19.040515383800873
9	17.37376366286021	23.29206865173942	41.409239364716136	17.924928320684234
10-11	19.064952014404668	32.436029230607474	32.86404198698947	15.634976767998388
12-13	19.230584076076674	23.306254544720765	40.12336365777831	17.33979772142424
14-15	18.869887583308635	27.81675079809881	38.03709565657984	15.276265962012722
16-17	20.503941347710143	28.375899331522263	35.52056910809141	15.599590212676183
18-19	20.127200480135606	28.756086677161345	32.94637036695527	18.17034247574778
20-21	21.647318289701335	28.501454620585665	32.38269730489222	17.46852978482078
22-23	21.273731017927275	30.203456485627516	31.97946400812377	16.543348488321442
24-25	20.922569028127977	29.599081502588593	31.086701716473858	18.39164775280957
26-27	20.866757128083425	30.230427347154304	31.117821563614957	17.78499396114732
28-29	21.690701195377613	29.513952658623506	30.922668447284263	17.872677698714618
30-31	20.680601034839988	31.29731528182407	30.039001322990543	17.9830823603454
32-33	20.703392481904558	29.99001053724235	31.025245387090987	18.281351593762103
34-35	21.753475947719274	30.52517430438589	29.384126837579284	18.33722291031555
36-37	21.74474721005395	30.54035159924312	29.730248904125684	17.984652286577248
38-39	22.478346255752243	28.573171013285215	30.803683198074573	18.144799532887973
40-41	22.144837652239254	30.624284641704424	28.810315838113677	18.42056186794265
42-43	22.375518598428272	30.074471866739387	29.41631653896874	18.133692995863594
44-45	22.58966428671379	29.210634051234884	29.72191302543087	18.47778863662046
46-47	21.127491193412904	30.210731842167416	29.50495150170492	19.156825462714767
48-49	21.463312150328203	29.338272402880232	30.197213237623178	19.001202209168383
50-51	20.97333108597811	29.608217371771445	30.903085845092743	18.5153656971577
52-53	23.219775327951066	28.230510843845774	28.698912992299242	19.85080083590392
54-55	22.16445129149231	28.55549538095486	30.27084871905079	19.009204608502035
56-57	22.590203652117662	29.001753394755127	29.495136004404586	18.912906948722625
58-59	22.08571715053155	29.480992590435367	29.031498164338558	19.401792094694517
60-61	22.65193653958005	28.946474573310592	28.79097194709735	19.610616940012008
62-63	23.226388098297342	28.362029319055747	29.057524065099404	19.354058517547504
64-65	22.843111445841583	28.744679939616706	29.498586528579363	18.91362208596235
66-67	23.14417091628332	28.42940876186781	28.885249070899505	19.54117125094937
68-69	23.474731149382556	27.78344956791596	29.421137215703475	19.320682066998014
70-71	22.38590637953695	27.95983905731898	29.72240904115731	19.93184552198676
72-73	23.09292960882396	27.259893426221538	29.67659998132733	19.97057698362717
74-75	23.749721853968875	27.395378656505375	29.509939847693335	19.344959641832414
76-77	22.15842196383738	27.652375301813553	29.745350930888137	20.443851803460927
78-79	22.04702576268011	28.22909497535648	29.586874343589812	20.13700491837359
80-81	22.00040837201101	27.844729400818274	30.35717923238428	19.797682994786435
82-83	22.36599388667076	28.645063780515507	28.809952413150903	20.178989919662826
84-85	22.29316969921934	28.658493748113905	28.74934226808871	20.298994284578047
86-87	22.346795337780716	29.422159715099504	29.351039286541248	18.88000566057853
88-89	23.46583048613555	27.142854432162444	30.160643101348082	19.23067198035393
90-91	23.67453830387288	27.45677083703298	28.765341565651394	20.10334929344275
92-93	23.10001279679813	27.547201049499304	29.063189771072096	20.289596382630474
94-95	23.93640862779615	27.78302630468989	28.073105811642012	20.207459255871946
96-97	23.27135385847211	27.649660938723812	29.361664620241935	19.71732058256214
98-99	25.003120394363076	26.636515247232634	28.741568548662876	19.618795809741417
100-101	25.054582507417283	26.871598059509193	28.0783810387764	19.99543839429712
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	93.0
1	184.5
2	361.5
3	643.0
4	1120.5
5	1765.0
6	2613.5
7	3704.0
8	5062.0
9	6468.5
10	7820.0
11	9379.5
12	11011.5
13	12593.5
14	14351.0
15	18679.0
16	29068.5
17	34087.5
18	39227.0
19	45871.5
20	54982.5
21	73656.5
22	95205.0
23	128029.5
24	173584.0
25	246052.5
26	309719.0
27	446141.5
28	579856.5
29	758361.0
30	963653.5
31	987823.0
32	1075537.0
33	1286032.5
34	1685283.0
35	1840869.0
36	1615003.5
37	1650234.5
38	1684971.0
39	1664708.0
40	1695071.0
41	1635128.5
42	1408134.5
43	1159437.5
44	1014688.0
45	859341.0
46	758737.0
47	719814.5
48	724428.0
49	709669.5
50	635316.5
51	576653.0
52	596123.0
53	594942.5
54	580048.0
55	504186.0
56	417822.5
57	408591.0
58	389468.0
59	298574.0
60	189859.0
61	129581.0
62	91728.0
63	66389.5
64	52928.0
65	43124.0
66	30941.0
67	18976.5
68	10176.0
69	7237.5
70	5523.5
71	8324.5
72	26894.5
73	63069.5
74	74594.5
75	52315.0
76	29249.5
77	64457.5
78	63878.0
79	11576.0
80	3150.0
81	825.0
82	421.0
83	296.5
84	288.5
85	292.5
86	250.5
87	164.0
88	96.5
89	87.0
90	76.5
91	62.0
92	48.0
93	31.5
94	30.0
95	28.5
96	23.0
97	26.0
98	24.5
99	28.0
100	70.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.24771461191401764
2	0.41518927138186984
3	0.41704365400380883
4	0.45117479075289363
5	0.4451713727991634
6	0.05187897797509523
7	0.015934278347321723
8	0.016432862354163812
9	0.025209107152963154
10-11	0.01674921536435309
12-13	0.15573752543543806
14-15	0.32056910652783793
16-17	0.20725495711317413
18-19	0.3307944522120204
20-21	0.1785149421690661
22-23	0.16435049126123646
24-25	0.12514895926128394
26-27	0.10637041951820514
28-29	0.10163970284509821
30-31	0.0843583729705189
32-33	0.09191002547181135
34-35	0.07845263083099463
36-37	0.11066232395137213
38-39	0.11361300824917445
40-41	0.12629336992026358
42-43	0.16585499036960208
44-45	0.17137294494824915
46-47	0.20258984359886217
48-49	0.22269648284554663
50-51	0.22206815036908775
52-53	0.23964688123020444
54-55	0.2898857802354244
56-57	0.29168039109046123
58-59	0.332233348161591
60-61	0.3814459221936623
62-63	0.3627271542174857
64-65	0.45744791058166995
66-67	0.41456968599325034
68-69	0.49322495769252284
70-71	0.5049373083093923
72-73	0.5494847688271517
74-75	0.6248715453704594
76-77	0.667434874796663
78-79	0.6462261024822223
80-81	0.7353108185819285
82-83	0.7595956501198686
84-85	0.7876971276517052
86-87	0.8558660988795884
88-89	0.8886705939730408
90-91	0.8715846157268732
92-93	0.9212797374380811
94-95	0.9573352335118196
96-97	0.9769112160962511
98-99	0.9886585550644778
100-101	1.0035023631278293
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.4297129E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.99059589770796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.02378436673548	9.356318777319169
2	10.158468528146589	2.6392911917747304
3	4.5066268120270605	1.7563130333045833
4	2.5066548645009297	1.3025176159902194
5	1.6728358900696727	1.0865567525538868
6	1.221714454350808	0.9522479267316076
7	0.9371487590253818	0.8521884577176062
8	0.6760898641235138	0.702624817229188
9	0.6025583428827783	0.7044832743464455
>10	4.430708343729239	11.64915338925271
>50	0.6114054397493444	5.556858771335401
>100	0.5093230309026652	13.528169764456678
>500	0.07126103260645288	6.414842021121283
>1k	0.05833467937064469	15.245382880938674
>5k	0.007217310929789005	6.554849974554109
>10k+	0.005868280849454611	21.69820135137375
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA	238478	0.6953293379163019	No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT	192269	0.5605979439270267	No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT	150330	0.4383165716290713	No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT	139564	0.40692618906964484	No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA	126789	0.3696781733538104	No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC	121715	0.35488393212154873	No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA	114789	0.3346898219964709	No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG	111320	0.3245752727582533	No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA	110822	0.3231232561769237	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	104606	0.3049992901738218	No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA	100617	0.29336857904345287	No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA	90034	0.2625117688422258	No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG	82630	0.2409239560547473	No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA	78133	0.2278120713835843	No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT	78014	0.22746510356595737	No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT	77410	0.2257040232143046	No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT	75151	0.21911746607128543	No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC	74355	0.21679657209791528	No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT	74353	0.2167907407060224	No Hit
CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA	71150	0.20745176658955913	No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG	68580	0.1999584280071956	No Hit
CTCAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCT	67178	0.19587062229027974	No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC	63749	0.18587270088991997	No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA	62750	0.18295992063942146	No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG	61605	0.17962144878074196	No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC	59319	0.17295616784716877	No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA	58641	0.1709793259954791	No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT	57860	0.1687021674613056	No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG	54702	0.15949439966243237	No Hit
CAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTG	53972	0.15736594162152756	No Hit
CAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTGTGTAGAAAT	53965	0.15734553174990246	No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT	51880	0.1512663057015647	No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG	51823	0.15110011103261733	No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT	50678	0.14776163917393786	No Hit
GTTTATTTTACACTGGGCGAATTGCTTCTGTACAATAGAAAGCAGTCTGT	49861	0.14537951558569231	No Hit
GTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCTTCTT	49190	0.14342308360562775	No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA	45891	0.1338042026783058	No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA	45623	0.13302279616465856	No Hit
GTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCT	44509	0.1297747108803189	No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGC	43800	0.1277074824542894	No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA	43257	0.1261242595553698	No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC	42471	0.12383252254146405	No Hit
CAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGT	42220	0.12310068285890635	No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA	41441	0.12082935571662572	No Hit
GTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAAT	41321	0.12047947220305234	No Hit
CGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAG	41276	0.12034826588546231	No Hit
GTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTG	40843	0.11908576954065164	No Hit
GAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGT	39653	0.11561609136438213	No Hit
CAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCA	38845	0.11326020903965461	No Hit
CATAAATTAAGTTGAGAGCGCTTATTTGTAAGGTGGCTCTATTTCTCTTG	37861	0.11039116422835275	No Hit
CGAACGTTTATGTTTATTGTGGATTGGCCACAGCGAGGGTCTGCTGGAGA	37483	0.10928903116059656	No Hit
GTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGTGGGTCACCTGA	37164	0.10835892415368062	No Hit
CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT	36114	0.10529744340991341	No Hit
CTTTAATACCTTTTTAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAAT	36043	0.10509042899771583	No Hit
CTTAATTATTATGATGAAGTTGGAGTAATTAATCTTGATGGTTTGGGAGA	35733	0.10418656325431788	No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG	35702	0.10409617667997807	No Hit
TGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACA	35383	0.10316606967306213	No Hit
AACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCC	34298	0.10000253957116935	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.831391892889927E-6	0.0	0.0	0.0	0.0
2	5.831391892889927E-6	0.0	0.0	0.0	0.0
3	5.831391892889927E-6	0.0	0.0	0.0	2.9156959464449636E-6
4	1.1662783785779854E-5	0.0	0.0	0.0	2.9156959464449636E-6
5	1.4578479732224816E-5	0.0	0.0	0.0	2.9156959464449636E-6
6	1.749417567866978E-5	0.0	0.0	0.0	2.9156959464449636E-6
7	2.0409871625114743E-5	0.0	0.0	0.0	2.9156959464449636E-6
8	2.0409871625114743E-5	0.0	0.0	0.0	2.9156959464449636E-6
9	2.0409871625114743E-5	0.0	0.0	2.9156959464449636E-6	2.9156959464449636E-6
10-11	2.1867719598337226E-5	0.0	0.0	2.9156959464449636E-6	2.9156959464449636E-6
12-13	3.20726554108946E-5	0.0	0.0	2.9156959464449636E-6	4.373543919667445E-6
14-15	4.9566831089564375E-5	0.0	0.0	2.9156959464449636E-6	5.831391892889927E-6
16-17	6.41453108217892E-5	0.0	0.0	2.9156959464449636E-6	5.831391892889927E-6
18-19	8.747087839334889E-5	0.0	0.0	2.9156959464449636E-6	8.74708783933489E-6
20-21	1.3995340542935825E-4	0.0	0.0	2.9156959464449636E-6	1.1662783785779854E-5
22-23	2.8282250680516143E-4	0.0	0.0	2.9156959464449636E-6	1.1662783785779854E-5
24-25	6.210432365927772E-4	0.0	0.0	2.9156959464449636E-6	1.1662783785779854E-5
26-27	0.0011006752197829737	0.0	0.0	2.9156959464449636E-6	1.1662783785779854E-5
28-29	0.002067228426029479	0.0	0.0	2.9156959464449636E-6	1.1662783785779854E-5
30-31	0.004127167612192846	0.0	0.0	2.9156959464449636E-6	1.1662783785779854E-5
32-33	0.008398662173734717	0.0	0.0	2.9156959464449636E-6	1.1662783785779854E-5
34-35	0.014919616157958877	0.0	0.0	2.9156959464449636E-6	1.1662783785779854E-5
36-37	0.024366471024440558	0.0	0.0	2.9156959464449636E-6	1.1662783785779854E-5
38-39	0.0377990822497125	0.0	0.0	2.9156959464449636E-6	1.1662783785779854E-5
40-41	0.05355112960038142	0.0	0.0	5.831391892889927E-6	1.1662783785779854E-5
42-43	0.07208183518801238	0.0	0.0	5.831391892889927E-6	1.4578479732224816E-5
44-45	0.09429798045194979	0.0	0.0	5.831391892889927E-6	1.4578479732224816E-5
46-47	0.11882481475344481	0.0	0.0	5.831391892889927E-6	1.4578479732224816E-5
48-49	0.14824564470104773	0.0	0.0	8.74708783933489E-6	1.4578479732224816E-5
50-51	0.17999611570986013	0.0	0.0	8.74708783933489E-6	1.4578479732224816E-5
52-53	0.21858243586511278	0.0	0.0	1.0204935812557372E-5	1.4578479732224816E-5
54-55	0.2679670359580244	0.0	0.0	1.60363277054473E-5	1.4578479732224816E-5
56-57	0.31926724828775027	0.0	0.0	1.749417567866978E-5	1.4578479732224816E-5
58-59	0.37145820572911514	0.0	0.0	1.8952023651892264E-5	1.4578479732224816E-5
60-61	0.42971235289111226	0.0	0.0	2.332556757155971E-5	1.4578479732224816E-5
62-63	0.5053338429581089	0.0	0.0	2.332556757155971E-5	1.4578479732224816E-5
64-65	0.5862050435766796	0.0	0.0	2.7699111491227153E-5	1.4578479732224816E-5
66-67	0.6757431503960579	0.0	0.0	2.9156959464449632E-5	1.4578479732224816E-5
68-69	0.7790579205623888	0.0	0.0	3.061480743767212E-5	1.4578479732224816E-5
70-71	0.8832109533133226	0.0	0.0	3.20726554108946E-5	1.4578479732224816E-5
72-73	0.9996667651102809	0.0	0.0	3.4988351357339556E-5	1.4578479732224816E-5
74-75	1.145759168354879	0.0	0.0	3.790404730378452E-5	1.8952023651892264E-5
76-77	1.2996437690163511	0.0	0.0	4.373543919667445E-5	2.0409871625114743E-5
78-79	1.4754733552187416	0.0	0.0	4.373543919667445E-5	2.1867719598337226E-5
80-81	1.6737027755296952	0.0	0.0	4.373543919667445E-5	2.332556757155971E-5
82-83	1.8907092777357546	0.0	0.0	4.373543919667445E-5	2.624126351800467E-5
84-85	2.1897415961551765	0.0	0.0	4.373543919667445E-5	3.061480743767212E-5
86-87	2.4909300717269947	0.0	0.0	4.373543919667445E-5	3.20726554108946E-5
88-89	2.8413500733545365	0.0	0.0	4.373543919667445E-5	3.20726554108946E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	25910	0.0	78.7045	1
GCGTGTT	31505	0.0	63.671913	2
GTAAACG	10845	0.0	59.626026	1
TAAACGC	12080	0.0	53.569717	2
CGAACGT	7720	0.0	52.805267	1
TCGAACG	3435	0.0	49.24163	2
CGTGTTT	44520	0.0	44.96225	3
GTCGAAC	3915	0.0	43.568233	1
CTTACGG	11815	0.0	39.04746	1
TTACGGT	11930	0.0	38.51192	2
CCAAGAC	18965	0.0	36.576935	1
GTAGATA	34640	0.0	36.402584	2
TAGATAT	35195	0.0	36.15275	3
GAACGTT	11525	0.0	35.82515	2
GTTTTTT	245115	0.0	35.410202	1
GATATAG	35845	0.0	35.31709	5
TACGGTC	13230	0.0	34.404667	3
AAACGCT	19270	0.0	33.65601	3
AGATATA	38030	0.0	33.59512	4
CTCAGTT	46900	0.0	33.44105	1
>>END_MODULE