FastQCFastQC Report
Fri 27 May 2016
SRR1233130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233130_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34297129
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC3540601.0323313067983038No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA1906610.5559095048451431No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT1888480.5506233480942384No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA1514990.44172502019046544No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT1350260.3936947608646776No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT1088860.3174784688246063No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT1050700.3063521730929723No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC1033490.3013342603691405No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC1028200.2997918572134711No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1026430.2992757790309504No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA943360.27505509280383206No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC843830.24603517104886535No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA809860.23613055191879181No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG762130.22221393516641No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG761450.22201566784205173No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC738250.21525125324629943No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT699800.20404040233221854No Hit
CTGGTATCTTTCAGAGTGCCAAGTGATTTCAGAATGCAAAGAATTCTTTG692270.20184488328454547No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG680760.1984889172501873No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT632780.1844994080991444No Hit
CTATATTCATAGCACTTATACTAAATAACTCTATAACCATCAACTCAATC589180.17178697377264435No Hit
AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA583840.17022999213724274No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA567990.16560861406212748No Hit
CTTTTGTATTGGTATTGTATGATGAGATCAAAAAATATGTGTAATACCCA558890.16295533075086255No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCC518540.15119049760695713No Hit
CCAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACC491940.14343474638941353No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA483720.14103804432143577No Hit
CTAACTTTGTGTCCGTCTCCATCCATGATTGTCTGTCTCACTGAGCCAAC482490.14067941372002304No Hit
CTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCA478190.1394256644630517No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC471490.13747214817893358No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT456230.13302279616465856No Hit
CTAACACTAGCATTATGTATGTGACATATTTCTTTACCAATTTTTACAGC449250.13098764039403998No Hit
ATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACT448530.13077771028589594No Hit
CTGCAGTATAGATTTGTGGTGCTAACTTTGTGTCCGTCTCCATCCATGAT435100.12686193062982035No Hit
CACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGG406060.11839474960134418No Hit
CTCAGCGTCAGTTCAGTTGCTCACAGCTTCTCACAGCGCATGTTTGACTG402590.11738300310792779No Hit
CCTAACACTAGCATTATGTATGTGACATATTTCTTTACCAATTTTTACAG398880.1163012799117967No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG386540.11270331111388361No Hit
CCCAGATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGC382870.1116332507015383No Hit
GGAAGATTGCAAAAGATGAAGGAGCCAACGCTTTCTTCAAAGGTGCTTGG381580.1112571259244469No Hit
CCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTA359460.10480760649091066No Hit
CCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTT352320.10272579958514895No Hit
CTCGTACCAACACATGATCTAGGAGGCTGCTGACCTCCAACAGGAATTTC349900.10202020116610927No Hit
TCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCA349890.10201728547016282No Hit
CTCACTTCTATGAAATAAAACTCCAGCAATACTAACTATAATCTCACTGA349520.10190940472014436No Hit
CTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTA348570.10163241360523209No Hit
CTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGA344110.10033201321311763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA202050.055.894931
TACGCAT154300.049.9688389
CGGCAAT156300.049.4701961
CTACGCA158100.048.707228
CTCTACG161700.047.5951166
TCCGCTC26400.044.9814382
TCGTACC81150.044.485892
CTCGTAC83600.043.967081
CGTACCA88000.041.0770453
TCCACTC876850.040.942922
CCGCAAA80550.040.725861
TCTACGC207350.040.66797
TAACGGC44950.040.5833789
GGCAATC190650.040.3124282
CTCCACT916050.039.7299581
CTCCGCT34350.039.0875971
CCACTCA943400.038.1302223
CCGAAAC298550.037.9303132
CGCAAAC84300.037.8650862
CCCCTCT215950.036.5550961
Produced by FastQC (version 0.11.5)