FastQCFastQC Report
Fri 27 May 2016
SRR1233129_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233129_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18125858
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC2157441.1902553799108433No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA1114380.6148012414088205No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT1105640.6099794006992663No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA807490.44549063553294965No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT751550.41462864819971557TruSeq Adapter, Index 15 (97% over 40bp)
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT686870.3789448201569272No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT661260.36481583382149413No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC629540.3473159725735466No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT605490.334047635151947No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT560720.30934811472096935No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA557380.30750544332853097No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC508370.28046672328559563No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA495570.27340498860798756No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC474500.2617807112910186No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG455780.2514529243250168No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC427110.23563574204321802No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG419180.23126077673123113No Hit
CTGGTATCTTTCAGAGTGCCAAGTGATTTCAGAATGCAAAGAATTCTTTG400030.22069575961590343No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG397590.2193496164429844No Hit
CTATATTCATAGCACTTATACTAAATAACTCTATAACCATCAACTCAATC359840.1985230161242574No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT346910.19138956070382987No Hit
AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA342630.18902829317100464No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT326190.17995837769445175No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA304050.16774378349427652No Hit
CTTTTGTATTGGTATTGTATGATGAGATCAAAAAATATGTGTAATACCCA290900.16048895450907757No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCC290460.16024620737953482No Hit
CTAACACTAGCATTATGTATGTGACATATTTCTTTACCAATTTTTACAGC270070.1489970847173138No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT269610.14874330362733726No Hit
CTAACTTTGTGTCCGTCTCCATCCATGATTGTCTGTCTCACTGAGCCAAC269310.1485777942208308No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC267830.14776128114873238No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA266210.14686753035359762No Hit
ATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACT259080.14293392345896122No Hit
CACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGG243090.134112272092168No Hit
CCTAACACTAGCATTATGTATGTGACATATTTCTTTACCAATTTTTACAG241470.1332185212970332No Hit
CTCAGCGTCAGTTCAGTTGCTCACAGCTTCTCACAGCGCATGTTTGACTG240870.13288750248402034No Hit
CCAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACC239130.131927547926283No Hit
CTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCA236960.13073036321921977No Hit
CTGCAGTATAGATTTGTGGTGCTAACTTTGTGTCCGTCTCCATCCATGAT228280.12594162439096676No Hit
CCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTT216250.11930469719005853No Hit
GGAAGATTGCAAAAGATGAAGGAGCCAACGCTTTCTTCAAAGGTGCTTGG213900.11800820683909143No Hit
TCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCA213550.1178151125315006No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG207530.11449389044093801No Hit
ATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTG201090.11094095518126645No Hit
CACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCT192270.10607497862997713No Hit
GTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCAT191890.10586533338173565No Hit
CTCACTTCTATGAAATAAAACTCCAGCAATACTAACTATAATCTCACTGA189800.10471228451640745No Hit
CCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTA189370.10447505436708154No Hit
CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT188930.10423230723753878No Hit
CTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGA188400.10393990728604406No Hit
CGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTAT185860.1025385943109562No Hit
CTTTTGTTTGATGGTTGCGTTCTTGATCTTGTTTGCCATGTAACAGAAAT185120.102130337774907No Hit
GTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATC182720.10080626252285547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA113350.055.9465561
TACGCAT71500.049.8311239
CTCTACG71850.049.5251356
CTACGCA72800.048.679998
CGGCAAT87650.048.2881431
GTCGCGA1400.047.6091731
TCCGCTC13400.046.4381372
TCGTACC37400.045.0884252
CTCGTAC38500.044.270351
TCTACGC90950.043.1467367
TCCACTC496300.042.85962
CGTACCA40150.042.4734153
CCGCAAA46250.041.1755031
CTCCACT521250.041.1014441
TAACGGC26600.040.7191439
GGCAATC105800.040.27312
CCACTCA542000.039.1844443
CGCAAAC47150.038.5856132
CTGGTAT137850.038.163321
CTCCGCT21100.037.4555631
Produced by FastQC (version 0.11.5)