##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233128_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31564515
Sequences flagged as poor quality	0
Sequence length	101
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.735650048796884	34.0	31.0	34.0	31.0	34.0
2	32.885024655059645	34.0	33.0	34.0	31.0	34.0
3	32.98081776323824	34.0	33.0	34.0	31.0	34.0
4	36.24855021532883	37.0	37.0	37.0	35.0	37.0
5	36.24014153235049	37.0	37.0	37.0	35.0	37.0
6	36.27409310740241	37.0	37.0	37.0	35.0	37.0
7	36.31864310286409	37.0	37.0	37.0	35.0	37.0
8	36.333174198938266	37.0	37.0	37.0	35.0	37.0
9	38.21285082314745	39.0	39.0	39.0	37.0	39.0
10-11	38.169947581960315	39.0	39.0	39.0	37.0	39.0
12-13	38.08441568007619	39.0	39.0	39.0	37.0	39.0
14-15	39.58764386210274	41.0	40.0	41.0	37.5	41.0
16-17	39.50059970824833	41.0	40.0	41.0	37.0	41.0
18-19	39.38552193816379	41.0	40.0	41.0	37.0	41.0
20-21	39.33358678249927	41.0	40.0	41.0	37.0	41.0
22-23	39.24183915070452	41.0	40.0	41.0	37.0	41.0
24-25	39.19583426198692	41.0	40.0	41.0	37.0	41.0
26-27	39.08717615334815	41.0	40.0	41.0	36.0	41.0
28-29	39.02017917905597	41.0	40.0	41.0	36.0	41.0
30-31	38.91183034176194	41.0	39.0	41.0	36.0	41.0
32-33	38.86246908910211	41.0	39.0	41.0	36.0	41.0
34-35	38.81622660763202	41.0	39.0	41.0	36.0	41.0
36-37	38.70741769673952	41.0	39.0	41.0	35.5	41.0
38-39	38.60428780546763	41.0	39.0	41.0	35.0	41.0
40-41	38.574413546351025	41.0	39.0	41.0	35.0	41.0
42-43	38.59087647948971	41.0	39.0	41.0	35.0	41.0
44-45	38.65632910881095	41.0	39.0	41.0	35.0	41.0
46-47	38.618212144238555	41.0	39.0	41.0	35.0	41.0
48-49	38.565722204190365	41.0	39.0	41.0	35.0	41.0
50-51	38.52034802372221	41.0	39.0	41.0	35.0	41.0
52-53	38.405266531103045	41.0	39.0	41.0	35.0	41.0
54-55	38.28492758402909	41.0	39.0	41.0	34.5	41.0
56-57	38.196728905861534	41.0	39.0	41.0	34.0	41.0
58-59	38.04936258326795	40.0	38.0	41.0	34.0	41.0
60-61	37.91961750402311	40.0	38.0	41.0	34.0	41.0
62-63	37.733296012943654	40.0	37.5	41.0	33.5	41.0
64-65	37.48284396259534	40.0	37.0	41.0	33.0	41.0
66-67	37.25486450528386	40.0	37.0	41.0	33.0	41.0
68-69	36.98035057722255	39.0	36.0	41.0	32.5	41.0
70-71	36.62419159616424	39.0	35.5	41.0	32.0	41.0
72-73	36.2902185412955	38.5	35.0	40.5	32.0	41.0
74-75	35.86287964506979	37.0	35.0	40.0	31.0	41.0
76-77	35.641871718922346	37.0	35.0	39.0	32.0	41.0
78-79	35.296038716260966	36.5	35.0	39.0	32.0	40.5
80-81	34.894501737156425	36.0	35.0	38.5	31.5	39.0
82-83	34.50528625261627	36.0	35.0	37.0	31.0	39.0
84-85	34.12524491505731	35.0	35.0	37.0	31.0	39.0
86-87	33.816065065469886	35.0	35.0	36.5	31.0	37.0
88-89	33.55743121666846	35.0	35.0	36.0	31.0	37.0
90-91	33.32092161086587	35.0	35.0	36.0	31.0	37.0
92-93	33.10565856944103	35.0	34.0	35.5	30.0	36.0
94-95	32.94318244712457	35.0	34.0	35.0	30.0	36.0
96-97	32.85455371324413	35.0	34.0	35.0	30.0	36.0
98-99	32.725175897681304	35.0	34.0	35.0	30.0	36.0
100-101	32.04842344322414	34.5	33.0	35.0	27.5	35.5
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.14979812623226252
1101	2	0.10227578387350889
1101	3	0.10030226377294582
1101	4	0.0783396755543393
1101	5	0.06714053262078323
1101	6	0.13085080952129147
1101	7	0.09413230454464383
1101	8	0.12023956621479925
1101	9	0.15731674595052425
1101	10-11	0.18035290253688174
1101	12-13	0.18111233073699395
1101	14-15	0.2480672169536149
1101	16-17	0.2556263940763941
1101	18-19	0.21193368739927365
1101	20-21	0.18534975588252678
1101	22-23	0.2110242553704751
1101	24-25	0.19665045648272184
1101	26-27	0.12425909040002381
1101	28-29	0.12537175111032894
1101	30-31	0.07254211174513614
1101	32-33	0.061475901279543166
1101	34-35	0.036127969149937655
1101	36-37	-0.04011452803630533
1101	38-39	-0.033071269265903425
1101	40-41	-0.07296184455132959
1101	42-43	-0.06796600672047504
1101	44-45	-0.06589921123982379
1101	46-47	-0.06785067774257669
1101	48-49	-0.09108627778612544
1101	50-51	-0.06175166564912615
1101	52-53	-0.022114982944074768
1101	54-55	-0.029538530539909402
1101	56-57	-0.05571409971811647
1101	58-59	-0.06762602532889161
1101	60-61	-0.09001727060679343
1101	62-63	-0.08747497851783947
1101	64-65	-0.14681681965857507
1101	66-67	-0.15066550165191472
1101	68-69	-0.1549270048827509
1101	70-71	-0.17935171906369618
1101	72-73	-0.19763890606962065
1101	74-75	-0.24529022258519007
1101	76-77	-0.230410201023048
1101	78-79	-0.26239844794015
1101	80-81	-0.24458147979711953
1101	82-83	-0.22913637194240266
1101	84-85	-0.2086161405587461
1101	86-87	-0.2283946462224975
1101	88-89	-0.23021708723109668
1101	90-91	-0.2536942012641603
1101	92-93	-0.21480904891425467
1101	94-95	-0.20580115680262878
1101	96-97	-0.23812659038094353
1101	98-99	-0.2560184675712236
1101	100-101	-0.4824944095386634
1102	1	0.11348929571774136
1102	2	0.0808916690581043
1102	3	0.07200155367790728
1102	4	0.07022022181291021
1102	5	0.07146750828786708
1102	6	0.10005284040794749
1102	7	0.07817143691335104
1102	8	0.1053956258392077
1102	9	0.12653645100843391
1102	10-11	0.1443759273251075
1102	12-13	0.15307751285583038
1102	14-15	0.20087112072145175
1102	16-17	0.2087455812149912
1102	18-19	0.13712737747177073
1102	20-21	0.1361906423478345
1102	22-23	0.13316223941222916
1102	24-25	0.11319933700733031
1102	26-27	0.0645289423624078
1102	28-29	0.053027564279965134
1102	30-31	0.027491843490061285
1102	32-33	0.02842434598733945
1102	34-35	-0.0121620576189585
1102	36-37	-0.07400277008843403
1102	38-39	-0.13034246464729904
1102	40-41	-0.14679140664735968
1102	42-43	-0.12049037274675811
1102	44-45	-0.04326248101158825
1102	46-47	-0.03474775176177047
1102	48-49	-0.05088440341334177
1102	50-51	-0.04554663591915187
1102	52-53	-0.06023281634268329
1102	54-55	-0.0756403943465287
1102	56-57	-0.11217150999807757
1102	58-59	-0.12026141154097303
1102	60-61	-0.16288589300675937
1102	62-63	-0.15314726181468785
1102	64-65	-0.20680887145017834
1102	66-67	-0.2189221860515005
1102	68-69	-0.223448524246308
1102	70-71	-0.24316700068907693
1102	72-73	-0.24393097567639188
1102	74-75	-0.25286270006654377
1102	76-77	-0.2671038382913338
1102	78-79	-0.2502103037098067
1102	80-81	-0.27697236944531767
1102	82-83	-0.24495352490621514
1102	84-85	-0.2505039108299769
1102	86-87	-0.23361791417983113
1102	88-89	-0.22696474217772078
1102	90-91	-0.2287745474505556
1102	92-93	-0.2071194418968787
1102	94-95	-0.24851533734435094
1102	96-97	-0.27408016141331615
1102	98-99	-0.31429884532951746
1102	100-101	-0.5383878493400722
1103	1	0.14210451164711202
1103	2	0.11120405443919879
1103	3	0.08644071795849584
1103	4	0.07333119947121958
1103	5	0.08011255166153575
1103	6	0.12302973655338434
1103	7	0.09203127044524706
1103	8	0.08251141312616284
1103	9	0.09893511182563941
1103	10-11	0.11738551918128337
1103	12-13	0.14163227315984273
1103	14-15	0.18624168883341952
1103	16-17	0.20450650422798589
1103	18-19	0.13406724829544459
1103	20-21	0.1324086142287797
1103	22-23	0.1396431254135706
1103	24-25	0.10253584378687464
1103	26-27	0.09838586396084281
1103	28-29	0.04910238274394629
1103	30-31	0.031830556427706824
1103	32-33	0.06487489669371627
1103	34-35	-0.011271220996320608
1103	36-37	-0.02898333063816949
1103	38-39	-0.10408122878845916
1103	40-41	-0.10218395614683828
1103	42-43	-0.07936562931202218
1103	44-45	-0.02045721372264353
1103	46-47	-0.004873630191930545
1103	48-49	-0.017637813399851154
1103	50-51	-0.004033984808891944
1103	52-53	0.006983487001285482
1103	54-55	-0.036190374417863325
1103	56-57	-0.06424080046228653
1103	58-59	-0.07548115799309585
1103	60-61	-0.10149361386329758
1103	62-63	-0.1006314894514233
1103	64-65	-0.14493047828228356
1103	66-67	-0.18299899510232365
1103	68-69	-0.19607061014355054
1103	70-71	-0.17943520277297154
1103	72-73	-0.17758811355314919
1103	74-75	-0.20300768392797863
1103	76-77	-0.20970485299248764
1103	78-79	-0.19268604050839855
1103	80-81	-0.23303479871002253
1103	82-83	-0.22483006289247953
1103	84-85	-0.18816011513635544
1103	86-87	-0.17823132801207464
1103	88-89	-0.23310964880172236
1103	90-91	-0.2194030242168239
1103	92-93	-0.18530605955582047
1103	94-95	-0.2222281524413674
1103	96-97	-0.21642946559262555
1103	98-99	-0.2543618373370009
1103	100-101	-0.6097033798674616
1104	1	0.12174416532393906
1104	2	0.06437191184019753
1104	3	0.07164143115851118
1104	4	0.06037252684479455
1104	5	0.05945204003128168
1104	6	0.09071949618064679
1104	7	0.07173215212334583
1104	8	0.0626902010821695
1104	9	0.11135325100978122
1104	10-11	0.1321870808392589
1104	12-13	0.13499693892899245
1104	14-15	0.15905994905978815
1104	16-17	0.1683212433790331
1104	18-19	0.12413416821599554
1104	20-21	0.13068351480568197
1104	22-23	0.14313133087899388
1104	24-25	0.11438756890859025
1104	26-27	0.10459488995086019
1104	28-29	0.08432406792284297
1104	30-31	0.05445899048496017
1104	32-33	0.04443864260520769
1104	34-35	0.024097577999064868
1104	36-37	-0.022922009406450172
1104	38-39	-0.05918935078634746
1104	40-41	-0.11126004784708243
1104	42-43	-0.07118045569640685
1104	44-45	0.0023424110103178464
1104	46-47	0.004670833308075828
1104	48-49	-0.004114394794022758
1104	50-51	0.010662107865648807
1104	52-53	0.01537887488711931
1104	54-55	-0.02984718198544556
1104	56-57	-0.0508544494187646
1104	58-59	-0.05609417977665032
1104	60-61	-0.08489884881014831
1104	62-63	-0.10726638400792154
1104	64-65	-0.1643094848740887
1104	66-67	-0.13958093910554226
1104	68-69	-0.16014006226188826
1104	70-71	-0.18743650141264112
1104	72-73	-0.23363747600818385
1104	74-75	-0.22599011453401374
1104	76-77	-0.21009010758564273
1104	78-79	-0.17286803065768908
1104	80-81	-0.2549886298301729
1104	82-83	-0.21004209387027117
1104	84-85	-0.22847346083274545
1104	86-87	-0.19916524644210654
1104	88-89	-0.2245065948081617
1104	90-91	-0.2156583807017256
1104	92-93	-0.17948587887334355
1104	94-95	-0.2218934455385977
1104	96-97	-0.23324212131331024
1104	98-99	-0.2974134738534957
1104	100-101	-0.6158945460665279
1105	1	0.12865485764847762
1105	2	0.0862419171445552
1105	3	0.04177749196315261
1105	4	0.04892930155560293
1105	5	0.04834045575518786
1105	6	0.09888848890491886
1105	7	0.07702570881551196
1105	8	0.08006768943877773
1105	9	0.10007133304771543
1105	10-11	0.1134470671616441
1105	12-13	0.12804866381715385
1105	14-15	0.121183821993327
1105	16-17	0.16051047091882964
1105	18-19	0.10553044751329566
1105	20-21	0.10969631936869462
1105	22-23	0.11452566377340645
1105	24-25	0.11073541924285735
1105	26-27	0.10421116335970737
1105	28-29	0.09351031786997765
1105	30-31	0.09526214456984405
1105	32-33	0.09132138575241555
1105	34-35	0.06133549163250507
1105	36-37	0.02480768487392737
1105	38-39	-0.018296539517365318
1105	40-41	-0.08259618806160773
1105	42-43	-0.04365698601914403
1105	44-45	0.05023954684012466
1105	46-47	0.049042820355197136
1105	48-49	0.048410827983779825
1105	50-51	0.04952257527817494
1105	52-53	0.04079347173490788
1105	54-55	0.02911586582008141
1105	56-57	0.009103192174009678
1105	58-59	-8.241583718699985E-4
1105	60-61	-0.026194760107308923
1105	62-63	-0.08012789536255127
1105	64-65	-0.10317508914169338
1105	66-67	-0.1132114968511928
1105	68-69	-0.11149793970513144
1105	70-71	-0.15610120982143627
1105	72-73	-0.1560184584853772
1105	74-75	-0.16094203525376827
1105	76-77	-0.14273595325397537
1105	78-79	-0.16312142158512444
1105	80-81	-0.189651663769709
1105	82-83	-0.1676997338745494
1105	84-85	-0.1678619680006932
1105	86-87	-0.152764351036069
1105	88-89	-0.1491019475571278
1105	90-91	-0.19259926122208526
1105	92-93	-0.15186036081156828
1105	94-95	-0.1713319944097833
1105	96-97	-0.18182287347985238
1105	98-99	-0.22336601371075915
1105	100-101	-0.5451002784749761
1106	1	0.13299050863867024
1106	2	0.09314130824083122
1106	3	0.0994985629551195
1106	4	0.08911659556745377
1106	5	0.08405698641598036
1106	6	0.10740508547541339
1106	7	0.09511826475869611
1106	8	0.08393838709947232
1106	9	0.09628439728232507
1106	10-11	0.12234182612574784
1106	12-13	0.12465897085186839
1106	14-15	0.10861208862220906
1106	16-17	0.14361722663819165
1106	18-19	0.1281380526790059
1106	20-21	0.15402072524379662
1106	22-23	0.16206012474657427
1106	24-25	0.12129014390808379
1106	26-27	0.11301588171632915
1106	28-29	0.0683977415480328
1106	30-31	0.05400033212379185
1106	32-33	0.04511230803188937
1106	34-35	0.0035981122581176805
1106	36-37	-0.0303737650084841
1106	38-39	-0.10824120218544664
1106	40-41	-0.10502581194634075
1106	42-43	-0.07987727463843441
1106	44-45	-0.006946383293232827
1106	46-47	0.022004410376602834
1106	48-49	0.01451166745519572
1106	50-51	0.025435486794535223
1106	52-53	0.016765984897602948
1106	54-55	-0.006229163091880707
1106	56-57	-0.04474083430905296
1106	58-59	-0.07547556444366421
1106	60-61	-0.09392969796836326
1106	62-63	-0.12501078100475382
1106	64-65	-0.12573055911870057
1106	66-67	-0.13378921815028377
1106	68-69	-0.14061205943519184
1106	70-71	-0.1702411234012331
1106	72-73	-0.1623735020155621
1106	74-75	-0.18957600013479237
1106	76-77	-0.21154240113484235
1106	78-79	-0.1962628417656873
1106	80-81	-0.20732720124065906
1106	82-83	-0.2149762621906106
1106	84-85	-0.18327218774378196
1106	86-87	-0.21088806634682555
1106	88-89	-0.2183824558202616
1106	90-91	-0.21808468973048178
1106	92-93	-0.19940524752464484
1106	94-95	-0.23415713625183088
1106	96-97	-0.21180207700944464
1106	98-99	-0.2646539820499072
1106	100-101	-0.6249786716397487
1107	1	0.10181774579198333
1107	2	0.07697269931279749
1107	3	0.0617549977080003
1107	4	0.02876438571286144
1107	5	0.03540163865876167
1107	6	0.08817885014437365
1107	7	0.05174180565566644
1107	8	0.031226458033870585
1107	9	0.08739407144828704
1107	10-11	0.08217129304819082
1107	12-13	0.08984893035228936
1107	14-15	0.10077635479768077
1107	16-17	0.146551906297411
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2216	2	-0.3305151060897913
2216	3	-0.2889959138300071
2216	4	-0.18004567807124516
2216	5	-0.30742799748315264
2216	6	-0.2288588865383403
2216	7	-0.18873689627376677
2216	8	-0.1980240985089594
2216	9	-0.3089662063473497
2216	10-11	-0.305651613299581
2216	12-13	-0.31454607774037413
2216	14-15	-0.46886665833184793
2216	16-17	-0.5464211508393859
2216	18-19	-0.4203661578844091
2216	20-21	-0.48428471400400497
2216	22-23	-0.4776050741373439
2216	24-25	-0.5141900607527461
2216	26-27	-0.4337070481579417
2216	28-29	-0.44048438811061175
2216	30-31	-0.4231954783395935
2216	32-33	-0.4058357754140971
2216	34-35	-0.4160007878216234
2216	36-37	-0.41626648014641887
2216	38-39	-0.3802920727095227
2216	40-41	-0.3916233797389168
2216	42-43	-0.36967456143207755
2216	44-45	-0.4521219755814485
2216	46-47	-0.484950152778147
2216	48-49	-0.42766466738530085
2216	50-51	-0.37763509693522934
2216	52-53	-0.346762542734389
2216	54-55	-0.3895566430493318
2216	56-57	-0.3590164172560151
2216	58-59	-0.3570671413643325
2216	60-61	-0.32236048021528774
2216	62-63	-0.37777432361883
2216	64-65	-0.34629249703110787
2216	66-67	-0.40041351963198224
2216	68-69	-0.41707665598788424
2216	70-71	-0.3624568239575936
2216	72-73	-0.3434386292872347
2216	74-75	-0.32459743527194007
2216	76-77	-0.23440932776164658
2216	78-79	-0.2234697917578714
2216	80-81	-0.1584592118705288
2216	82-83	-0.18333170786242903
2216	84-85	-0.1337546011448154
2216	86-87	-0.11982305237224011
2216	88-89	-0.15964210728760264
2216	90-91	-0.2116878071355046
2216	92-93	-0.2131726647057377
2216	94-95	-0.1949865568944702
2216	96-97	-0.2258101639513299
2216	98-99	-0.22106777190839466
2216	100-101	-0.0731207358333279
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40470.0
3	12913.0
4	12823.0
5	21183.0
6	71545.0
7	65996.0
8	49801.0
9	58772.0
10	78478.0
11	76237.0
12	64027.0
13	44649.0
14	42219.0
15	38797.0
16	37225.0
17	37542.0
18	40249.0
19	37657.0
20	43996.0
21	54483.0
22	60523.0
23	72160.0
24	83940.0
25	96255.0
26	115638.0
27	152301.0
28	154774.0
29	171459.0
30	202213.0
31	258392.0
32	346388.0
33	495170.0
34	739984.0
35	1028949.0
36	1845312.0
37	5099682.0
38	1.2318728E7
39	7372433.0
40	21152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.53026071854665	18.862676849644068	18.42422051625147	37.182841915557816
2	14.642417950126829	22.49482857831459	54.47489900839516	8.38785446316342
3	13.75269267954784	23.186129011230797	46.75261884191866	16.308559467302704
4	17.238564327491705	31.878807960592415	37.127245823980836	13.755381887935037
5	19.854229055725163	36.81909191348064	31.83877717397617	11.487901856818032
6	15.706469789379017	37.95009922009745	33.158223692093244	13.185207298430294
7	13.447169092579939	16.483175559879395	55.718473103374066	14.351182244166601
8	14.892781442791266	22.53617475889541	43.7101077469288	18.860936051384524
9	17.526923908034853	23.73363935017624	41.08844621316991	17.650990528618998
10-11	19.068558367502035	33.15161939133569	32.625118711352115	15.154703529810156
12-13	19.178550274843886	23.745984208410924	40.04974823653249	17.025717280212703
14-15	18.827398061361226	28.525386283647986	37.557017981214294	15.090197673776492
16-17	20.42100776944136	28.97752342161769	35.26222727689394	15.33924153204701
18-19	19.936589966546936	29.386618468688365	32.61241594856125	18.064375616203456
20-21	21.54159079638077	29.202926088445956	32.11701630736941	17.13846680780386
22-23	21.052503896692887	31.04811495071868	31.75794469509201	16.141436457496418
24-25	20.75422992622338	30.18350762348556	30.95802008895078	18.10424236134028
26-27	20.512600014712422	30.809454332551244	31.263738090813526	17.414207561922808
28-29	21.347111051236396	30.07535112652631	31.126012875347563	17.45152494688973
30-31	20.514436443381477	31.856315766810788	30.13512312893302	17.49412466087471
32-33	20.334252201589525	30.43060335492087	31.267805120441714	17.967339323047888
34-35	21.403458592589537	30.964913194342884	29.58134204101583	18.050286172051745
36-37	21.475928965117912	31.114132365381696	29.78098026290082	17.62895840659957
38-39	22.187748182871996	29.138362513139548	30.936278968815916	17.73761033517254
40-41	21.86883208772094	31.066899661111435	28.88084550888869	18.18342274227893
42-43	22.24707703720965	30.6711445848313	29.36796910167285	17.713809276286206
44-45	22.405090498303	29.833641932772686	29.749431854110973	18.011835714813337
46-47	20.764538794073164	30.880637141270846	29.796107993157094	18.558716071498896
48-49	21.22764867264771	30.12101643523882	30.084433456884902	18.566901435228576
50-51	20.609438167397236	30.31885351381898	31.076613415501125	17.99509490328266
52-53	22.934156127079135	28.818864168543335	28.90449695261017	19.342482751767363
54-55	21.92188048564984	29.169119296786977	30.36381191165276	18.545188305910425
56-57	22.50524217215589	29.54546519756784	29.52541149297797	18.423881137298302
58-59	21.811515043038295	30.26791644741007	29.122796605319007	18.79777190423263
60-61	22.47037027548702	29.726440330440223	28.84620060484796	18.956988789224795
62-63	23.004244364777964	28.83560733223181	29.207903853882712	18.952244449107514
64-65	22.480212573298214	29.406810548468993	29.72239021655604	18.390586661676757
66-67	22.992958275797644	28.94292053906617	28.9949239741467	19.06919721098949
68-69	23.295079563274772	28.397863768049813	29.556224543825238	18.750832124850177
70-71	22.119617595888023	28.488170123114404	30.001881991490915	19.390330289506654
72-73	23.054198044467213	27.731128458686438	29.90636339445498	19.308310102391367
74-75	23.676345595110433	27.925467791528895	29.714294486832994	18.68389212652768
76-77	21.908346152600306	28.149043302543646	29.984084826825185	19.958525718030863
78-79	22.000243645523827	28.81649984070558	29.693095934149376	19.49016057962122
80-81	21.934941701707032	28.25202316179413	30.76281616429128	19.050218972207556
82-83	22.273415852351754	29.08393034240138	29.117682390244582	19.524971415002284
84-85	22.367982890660425	29.095972438189154	28.934096908021363	19.601947763129054
86-87	22.169584484841543	29.954140589312907	29.748159469802278	18.128115456043272
88-89	23.358884646703515	27.74979028093124	30.388405358047066	18.502919714318182
90-91	23.48305478382319	28.078950291904782	29.013451224123454	19.424543700148572
92-93	22.98469251021367	28.10613339603253	29.283121657459	19.6260524362948
94-95	23.751692202743417	28.395216021214353	28.275861451186813	19.577230324855417
96-97	22.96330734980311	28.156981002777375	29.73174727851941	19.147964368900105
98-99	24.846677245328696	27.14345435046196	28.84434165750122	19.16552674670812
100-101	24.816894457014406	27.201637107426933	28.407339457176047	19.574128978382614
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	59.0
1	133.5
2	289.5
3	504.0
4	902.5
5	1519.5
6	2336.0
7	3301.0
8	4506.5
9	5735.5
10	6741.0
11	8132.5
12	9585.5
13	10783.0
14	12274.5
15	14640.0
16	19061.0
17	22793.5
18	28436.5
19	34783.0
20	44681.0
21	63023.5
22	88164.0
23	124692.0
24	165584.0
25	233613.0
26	297956.0
27	447234.0
28	600274.5
29	797956.5
30	994827.0
31	993831.0
32	1070026.0
33	1260940.0
34	1615644.0
35	1756310.0
36	1542841.0
37	1547984.0
38	1574564.0
39	1573403.0
40	1599092.5
41	1544162.0
42	1325155.5
43	1074957.0
44	939796.5
45	787091.5
46	680806.0
47	644603.5
48	648262.0
49	618053.0
50	540204.0
51	476194.0
52	478509.0
53	466087.5
54	440358.5
55	382667.0
56	314219.0
57	295658.5
58	273602.0
59	211180.0
60	141303.0
61	97978.5
62	70844.0
63	54367.5
64	43872.5
65	34885.5
66	25082.0
67	15483.5
68	8444.5
69	5955.5
70	4473.5
71	7182.5
72	23315.5
73	54447.5
74	65216.0
75	46243.5
76	24932.5
77	52730.0
78	52277.5
79	9415.0
80	2621.0
81	663.0
82	358.0
83	273.5
84	269.0
85	306.0
86	262.5
87	152.0
88	78.0
89	58.5
90	51.0
91	37.5
92	28.0
93	21.5
94	16.5
95	15.5
96	17.5
97	19.0
98	21.0
99	24.5
100	45.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.24539265057612955
2	0.4125487117416504
3	0.41400287633122196
4	0.4483737513470427
5	0.44269332191544836
6	0.052182015152141575
7	0.016249259651225433
8	0.01674665363937954
9	0.02560470198892649
10-11	0.01677991884240895
12-13	0.15404640305735728
14-15	0.3181658264034787
16-17	0.2043513103242676
18-19	0.32860001175370507
20-21	0.17644180498258885
22-23	0.16237854438758206
24-25	0.12316520624505081
26-27	0.10562969207668803
28-29	0.10140184317737815
30-31	0.0847819141209678
32-33	0.092968322180778
34-35	0.07923296144420404
36-37	0.11299714251905978
38-39	0.11596566587511324
40-41	0.12903889066567314
42-43	0.1688430821762983
44-45	0.17495120707541365
46-47	0.20692698747311658
48-49	0.2272377700085048
50-51	0.22670394270274705
52-53	0.2444469683757219
54-55	0.2952619420890833
56-57	0.2978677163263874
58-59	0.33864135089672687
60-61	0.387772471713885
62-63	0.3689712957731174
64-65	0.46399572431257063
66-67	0.4211612312116945
68-69	0.49967344659026125
70-71	0.511998996341303
72-73	0.5572159116019999
74-75	0.6350073809149293
76-77	0.6787479547840352
78-79	0.657336252434102
80-81	0.7469384528797607
82-83	0.7712854133827179
84-85	0.799929921305618
86-87	0.8704521517279767
88-89	0.9034528171904431
90-91	0.8862531231669487
92-93	0.9366182246107694
94-95	0.9720076484622052
96-97	0.9917846036918356
98-99	1.003364062460646
100-101	1.0188814876452243
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.1564515E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.65736303995677
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.3575791326556	9.031987847344654
2	10.220660558779562	2.587332224012807
3	4.631851438331217	1.7588107560631245
4	2.6007293069543382	1.316735000271049
5	1.7741450031276942	1.1227998695056232
6	1.2478619557274364	0.9476785078435576
7	0.9183664607447376	0.8136868388156449
8	0.7337833796048204	0.7430210102675698
9	0.5944434864343239	0.6771678313083169
>10	4.612540114115004	11.778444001381825
>50	0.624870604225189	5.534813138671109
>100	0.5371929892525176	13.847011657477303
>500	0.07260810149723487	6.3757902253520085
>1k	0.059546290582883916	15.246008355508147
>5k	0.007761893423849691	6.861114731466751
>10k+	0.006059284543779436	21.357598004710624
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA	236039	0.7477985959866641	No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT	177150	0.5612314968248364	No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT	170606	0.5404993550510755	No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT	145919	0.46228811055706065	No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA	134027	0.4246128920403181	No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA	118091	0.37412581818538954	No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC	112750	0.3572049182444273	No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA	108996	0.34531181613276807	No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG	98292	0.31140031773021065	No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA	96970	0.3072120702630787	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	96423	0.30547911159097485	No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA	92967	0.29453010762243614	No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG	91501	0.289885651656615	No Hit
CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA	82288	0.2606978120842345	No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC	77168	0.2444770654641771	No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT	74971	0.2375167177445939	No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT	72255	0.22891211856098534	No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA	67900	0.21511497959021386	No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT	64888	0.20557261849263328	No Hit
CAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTGTGTAGAAAT	64551	0.20450496388111777	No Hit
CTCAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCT	62161	0.1969331700487082	No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG	60052	0.1902516164116572	No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT	58048	0.1839027148049004	No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA	57247	0.1813650550309422	No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA	56048	0.17756648565644045	No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC	55415	0.1755610691309529	No Hit
GTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCTTCTT	54781	0.1735524844908911	No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG	54582	0.17292202969061934	No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC	52119	0.16511896349429098	No Hit
CAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTG	48922	0.1549905012004778	No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG	48753	0.15445508983743295	No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT	47027	0.14898692408231207	No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT	46302	0.14669004101599534	No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT	45396	0.14381972921174302	No Hit
GTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTG	44862	0.1421279560291042	No Hit
CGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAG	43080	0.13648237585782644	No Hit
GTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCT	42294	0.13399223780248168	No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG	42283	0.13395738854216516	No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA	41271	0.13075125659304443	No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA	41172	0.13043761325019568	No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA	40858	0.12944282527388745	No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGC	40131	0.12713960597842228	No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA	39355	0.12468114906881984	No Hit
GTTTATTTTACACTGGGCGAATTGCTTCTGTACAATAGAAAGCAGTCTGT	39173	0.12410455221630999	No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC	39136	0.12398733197706349	No Hit
CAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCA	38876	0.12316362218776371	No Hit
GTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAAT	38705	0.12262187459557038	No Hit
GTTCTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTT	37343	0.11830690254546916	No Hit
GAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGT	36124	0.11444497087948287	No Hit
CTTAATTATTATGATGAAGTTGGAGTAATTAATCTTGATGGTTTGGGAGA	32927	0.1043165085856697	No Hit
CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT	32635	0.10339141912999454	No Hit
TGGAAATATTGTTAGTGAAGTGGAATAAATTAGGCGAGTATAAAAGAATA	32538	0.10308411201629425	No Hit
TTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGAT	32006	0.10139867506280391	No Hit
GATTTATTTACCTGAAATCATGTCTGTGCAATACCTGTTACCAGCACAGA	31996	0.10136699391706161	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.168114574229954E-6	0.0	0.0	3.168114574229954E-6	0.0
2	3.168114574229954E-6	0.0	0.0	3.168114574229954E-6	0.0
3	3.168114574229954E-6	0.0	0.0	3.168114574229954E-6	0.0
4	9.504343722689862E-6	0.0	0.0	3.168114574229954E-6	0.0
5	9.504343722689862E-6	0.0	0.0	3.168114574229954E-6	0.0
6	1.584057287114977E-5	0.0	0.0	6.336229148459908E-6	0.0
7	1.584057287114977E-5	0.0	0.0	6.336229148459908E-6	0.0
8	1.9008687445379723E-5	0.0	0.0	9.504343722689862E-6	0.0
9	1.9008687445379723E-5	0.0	0.0	1.2672458296919817E-5	0.0
10-11	1.9008687445379723E-5	0.0	0.0	1.2672458296919817E-5	0.0
12-13	2.217680201960968E-5	0.0	0.0	1.2672458296919817E-5	0.0
14-15	3.0097088455184563E-5	0.0	0.0	1.2672458296919817E-5	0.0
16-17	3.960143217787442E-5	0.0	0.0	1.2672458296919817E-5	0.0
18-19	6.17782341974841E-5	0.0	0.0	1.584057287114977E-5	0.0
20-21	9.345937993978366E-5	0.0	0.0	1.7424630158264746E-5	0.0
22-23	2.0117527546360207E-4	0.0	0.0	2.0592744732494703E-5	0.0
24-25	3.6116506146221476E-4	0.0	0.0	2.217680201960968E-5	0.0
26-27	6.478794304300257E-4	0.0	0.0	3.168114574229954E-5	0.0
28-29	0.0012387327985239121	0.0	0.0	3.168114574229954E-5	0.0
30-31	0.002433111993008605	0.0	0.0	4.435360403921936E-5	0.0
32-33	0.004940674678511613	0.0	0.0	5.0689833187679266E-5	0.0
34-35	0.008883393266140792	0.0	0.0	5.5442005049024196E-5	0.0
36-37	0.014635105275655272	0.0	0.0	6.494634877171406E-5	0.0
38-39	0.02321119142809576	0.0	0.0	7.128257792017397E-5	0.0
40-41	0.0337705173040042	0.0	0.0	7.603474978151889E-5	0.0
42-43	0.04630199450237078	0.0	0.0	8.237097892997881E-5	0.0
44-45	0.06187644574928523	0.0	0.0	8.553909350420877E-5	0.0
46-47	0.07927414693366902	0.0	0.0	8.712315079132375E-5	0.0
48-49	0.10065100002328564	0.0	0.0	9.029126536555369E-5	0.0
50-51	0.12397149140419234	0.0	0.0	1.1880429653362328E-4	0.0
52-53	0.1530832962267914	0.0	0.0	1.3622892669188803E-4	0.0
54-55	0.19224277642156073	0.0	0.0	1.39397041266118E-4	0.0
56-57	0.2334140093709661	0.0	0.0	1.4573327041457788E-4	0.0
58-59	0.27569408242135196	0.0	0.0	1.5523761413726775E-4	0.0
60-61	0.3257217796630172	0.0	0.0	1.6315790057284263E-4	0.0
62-63	0.392084275649412	0.0	0.0	1.663260151470726E-4	0.0
64-65	0.46213920917207185	0.0	0.0	1.7424630158264747E-4	0.0
66-67	0.5405484608269762	0.0	0.0	1.7741441615687744E-4	0.0
68-69	0.6336149945595553	0.0	0.0	1.7741441615687744E-4	0.0
70-71	0.7249327290471594	0.0	0.0	1.789984734439924E-4	0.0
72-73	0.8280105048343052	0.0	0.0	1.8216658801822237E-4	0.0
74-75	0.9602919607667028	0.0	0.0	1.8850281716668225E-4	0.0
76-77	1.099527428189535	0.0	0.0	2.0117527546360207E-4	0.0
78-79	1.2559451650057034	0.0	0.0	2.2652019205744172E-4	0.0
80-81	1.435800613442025	0.0	0.0	2.3760859306724656E-4	0.0
82-83	1.6356326083261536	0.0	0.0	2.407767076414765E-4	0.0
84-85	1.9183789137897413	0.0	0.0	2.407767076414765E-4	0.0
86-87	2.2012392713780016	0.0	0.0	2.407767076414765E-4	0.0
88-89	2.530930698602529	0.0	0.0	2.4394482221570647E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	18580	0.0	78.64356	1
GCGTGTT	22630	0.0	63.87663	2
GTAAACG	9640	0.0	57.30931	1
TAAACGC	10125	0.0	54.798973	2
TCGAACG	2345	0.0	53.276726	2
CGAACGT	5140	0.0	50.737812	1
GTCGAAC	2910	0.0	43.74862	1
CGTGTTT	33935	0.0	42.541313	3
CTCAGTT	48190	0.0	37.5274	1
TAGATAT	31100	0.0	37.498955	3
CTTACGG	10305	0.0	36.324593	1
GTAGATA	32105	0.0	36.16206	2
TTACGGT	10440	0.0	35.90055	2
GATATAG	33435	0.0	34.389244	5
ACGGTCT	11210	0.0	33.985744	4
GTTTTTT	206325	0.0	33.955006	1
AAACGCT	16680	0.0	33.434906	3
AGATATA	35460	0.0	33.182976	4
CCAAGAC	13935	0.0	32.521004	1
GTAGTTG	26335	0.0	31.963804	3
>>END_MODULE