FastQCFastQC Report
Fri 27 May 2016
SRR1233128_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233128_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31564515
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC3605681.142320735800946No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA2180560.6908263915982868No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT2028980.6428041108821092No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT1469390.46551958742277527No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA1432170.4537278649774913No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC1147190.3634429358410861No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT1122040.3554751276868978No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC1106340.3505011878053568No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT915760.29012326024968227No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT903680.2862961778440125No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA897750.2844174859014941No Hit
CTATATTCATAGCACTTATACTAAATAACTCTATAACCATCAACTCAATC813010.2575708829994695No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC798350.25292642703364837No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT758170.24019694267439243No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT710240.22501216952010827No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG703700.2229402225885619No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC700180.2218250462584329No Hit
AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA640600.20294941962517085No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG639610.20263577628232213No Hit
CTAACTTTGTGTCCGTCTCCATCCATGATTGTCTGTCTCACTGAGCCAAC632620.20042126419493536No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA631570.2000886121646412No Hit
CTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCA582360.1844983203448556No Hit
CTGGTATCTTTCAGAGTGCCAAGTGATTTCAGAATGCAAAGAATTCTTTG549830.17419244363488556No Hit
CCAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACC519030.16443465074625732No Hit
CTTTTGTATTGGTATTGTATGATGAGATCAAAAAATATGTGTAATACCCA508320.161041600037257No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA491850.15582371533350028No Hit
CTAACACTAGCATTATGTATGTGACATATTTCTTTACCAATTTTTACAGC475590.1506723610358024No Hit
CTGCAGTATAGATTTGTGGTGCTAACTTTGTGTCCGTCTCCATCCATGAT456600.1446561114593397No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA450110.14260000510066445No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG447550.1417889677696616No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC440200.13946040355760259No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT437610.13863986188287702No Hit
CTCAGCGTCAGTTCAGTTGCTCACAGCTTCTCACAGCGCATGTTTGACTG430380.13634931504570877No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCC422520.13385917699036404No Hit
ATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTG412950.13082729134282595No Hit
ATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACT410530.1300606076158623No Hit
GTCCGTCTCCATCCATGATTGTCTGTCTCACTGAGCCAACTTAACTCTGA399530.12657568158420937No Hit
CCTAACACTAGCATTATGTATGTGACATATTTCTTTACCAATTTTTACAG390800.1238099175609066No Hit
CTTTTGTTTGATGGTTGCGTTCTTGATCTTGTTTGCCATGTAACAGAAAT387570.12278661655343033No Hit
CCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTT382300.12111702017281116No Hit
CTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTA380950.1206893247052901No Hit
CTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGA379180.1201285684256514No Hit
CCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTA377160.11948860928165696No Hit
CTCACTTCTATGAAATAAAACTCCAGCAATACTAACTATAATCTCACTGA376390.11924466445944124No Hit
CTCGTACCAACACATGATCTAGGAGGCTGCTGACCTCCAACAGGAATTTC364710.11554430663674065No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG361150.11441645784831479No Hit
GGAAGATTGCAAAAGATGAAGGAGCCAACGCTTTCTTCAAAGGTGCTTGG360160.11410281450546603No Hit
CTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAATAGA357510.1132632641432951No Hit
CACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGG356160.11283556867577405No Hit
CCCAAATAAATCTGTACTCAGAAAAAGCAAATCCATATGAATGCGGATTC350810.11114062737856102No Hit
CCGCAAACAAAGATCACCCAGCTACTACCATCATTCAAGTAGCACAACTA350540.11105508828505681No Hit
CGAGGACTTCAAATGGTCTTCCCACTTATAGCCACATGATGACTGATAGC342950.10865048932321628No Hit
CACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCT341990.10834635032409021No Hit
CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT332080.10520674878102831No Hit
CCCGTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGA331660.10507368796891066No Hit
TAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCA323750.10256770934069476No Hit
TAACAATTAAACCTAAACCTCCATAAATAGGTGAAGGCTTTAATGCTAAC319660.1012719504798347No Hit
CAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACA315670.10000787276471697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA162100.056.9566461
TACGCAT159950.050.2224359
TCGTACC81150.049.4033662
CTCTACG163050.049.065186
CTACGCA165150.048.582738
CGGCAAT96900.047.9104731
CTCGTAC86000.047.6710051
CGTACCA87100.045.9194373
CCGCAAA78350.045.7620621
TAACGGC54050.044.2099
TCCACTC829650.044.13732
TCCGCTC24600.044.0253032
TCTACGC200750.042.7616777
CGACCGT2900.042.689881
CTCCACT872900.042.4116821
CCACTCA892200.040.952433
CGCAAAC87950.040.020732
CCCCTCT208900.038.9541051
CTCCGCT36700.038.663381
ACTCATG950450.038.6286055
Produced by FastQC (version 0.11.5)