##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233127_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29655924
Sequences flagged as poor quality	0
Sequence length	101
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74720561733298	34.0	31.0	34.0	31.0	34.0
2	32.92055739015247	34.0	33.0	34.0	31.0	34.0
3	32.98838859311886	34.0	33.0	34.0	31.0	34.0
4	36.24701486286517	37.0	37.0	37.0	35.0	37.0
5	36.23457060383618	37.0	37.0	37.0	35.0	37.0
6	36.27266410582924	37.0	37.0	37.0	35.0	37.0
7	36.2999132315014	37.0	37.0	37.0	35.0	37.0
8	36.334761749456874	37.0	37.0	37.0	35.0	37.0
9	38.21497940849862	39.0	39.0	39.0	37.0	39.0
10-11	38.16568782345139	39.0	39.0	39.0	37.0	39.0
12-13	38.10459803916412	39.0	39.0	39.0	37.0	39.0
14-15	39.56196360632702	41.0	40.0	41.0	37.5	41.0
16-17	39.51292984497802	41.0	40.0	41.0	37.0	41.0
18-19	39.407072074368685	41.0	40.0	41.0	37.0	41.0
20-21	39.29866636426503	41.0	40.0	41.0	37.0	41.0
22-23	39.20585298573061	41.0	40.0	41.0	37.0	41.0
24-25	39.146998673856864	41.0	40.0	41.0	36.0	41.0
26-27	39.03331752536188	41.0	40.0	41.0	36.0	41.0
28-29	38.93174783560951	41.0	39.5	41.0	36.0	41.0
30-31	38.85031946736848	41.0	39.0	41.0	36.0	41.0
32-33	38.79576343667458	41.0	39.0	41.0	36.0	41.0
34-35	38.69369170557626	41.0	39.0	41.0	36.0	41.0
36-37	38.58523578628	41.0	39.0	41.0	35.0	41.0
38-39	38.52975909636132	41.0	39.0	41.0	35.0	41.0
40-41	38.49024881167081	41.0	39.0	41.0	35.0	41.0
42-43	38.489376372154176	41.0	39.0	41.0	35.0	41.0
44-45	38.56765767945723	41.0	39.0	41.0	35.0	41.0
46-47	38.53684483073263	41.0	39.0	41.0	35.0	41.0
48-49	38.49105591854093	41.0	39.0	41.0	35.0	41.0
50-51	38.43696444932891	41.0	39.0	41.0	35.0	41.0
52-53	38.32133277991945	41.0	39.0	41.0	34.5	41.0
54-55	38.20510155070535	41.0	39.0	41.0	34.0	41.0
56-57	38.10913293411461	40.5	39.0	41.0	34.0	41.0
58-59	37.9556975024619	40.0	38.0	41.0	34.0	41.0
60-61	37.82592095258944	40.0	38.0	41.0	34.0	41.0
62-63	37.63364183156121	40.0	37.5	41.0	33.0	41.0
64-65	37.3730575381836	40.0	37.0	41.0	33.0	41.0
66-67	37.14410522160766	39.5	36.5	41.0	33.0	41.0
68-69	36.868922006948765	39.0	36.0	41.0	32.0	41.0
70-71	36.51405322929746	39.0	35.5	41.0	32.0	41.0
72-73	36.18990423296202	38.5	35.0	40.5	31.5	41.0
74-75	35.764900210157	37.0	35.0	39.5	31.0	41.0
76-77	35.540016473605746	37.0	35.0	39.0	32.0	41.0
78-79	35.19992998700698	36.5	35.0	39.0	32.0	40.5
80-81	34.80017398884621	36.0	35.0	38.5	31.0	39.0
82-83	34.412864222338854	35.5	35.0	37.0	31.0	39.0
84-85	34.03972887508074	35.0	35.0	37.0	31.0	39.0
86-87	33.73368388386751	35.0	35.0	36.5	31.0	37.0
88-89	33.481511467995404	35.0	35.0	36.0	31.0	37.0
90-91	33.251638677655095	35.0	35.0	36.0	30.5	37.0
92-93	33.03684852308092	35.0	34.0	35.5	30.0	36.0
94-95	32.878599382032405	35.0	34.0	35.0	30.0	36.0
96-97	32.793303675178024	35.0	34.0	35.0	30.0	36.0
98-99	32.66597892886426	35.0	34.0	35.0	30.0	36.0
100-101	31.984740923938162	34.5	33.0	35.0	27.5	35.5
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1356497126318459
1101	2	0.07245820747233012
1101	3	0.0670170325024273
1101	4	0.03792301739372306
1101	5	0.02949749253922107
1101	6	0.09635443987727399
1101	7	0.05051946098512872
1101	8	0.08465191561106877
1101	9	0.11818388274037517
1101	10-11	0.14044881998639624
1101	12-13	0.15347336238130538
1101	14-15	0.2135744943947202
1101	16-17	0.24613741905341158
1101	18-19	0.1546484506572341
1101	20-21	0.12766418173767846
1101	22-23	0.13711214776767378
1101	24-25	0.11499297549157461
1101	26-27	0.040527360882514074
1101	28-29	0.03604730435306891
1101	30-31	-0.032715955360892224
1101	32-33	-0.004344372314278644
1101	34-35	-0.028898818997717513
1101	36-37	-0.09589331432542991
1101	38-39	-0.11521512602914896
1101	40-41	-0.15057038165527104
1101	42-43	-0.14279081860139797
1101	44-45	-0.11486846656011807
1101	46-47	-0.12781066803023577
1101	48-49	-0.14196554972525632
1101	50-51	-0.10873848487113946
1101	52-53	-0.05481699261282813
1101	54-55	-0.10543447575735598
1101	56-57	-0.13169106249038975
1101	58-59	-0.12543987406098722
1101	60-61	-0.1591036036945681
1101	62-63	-0.15992591345885643
1101	64-65	-0.22490330609414855
1101	66-67	-0.22472435382292844
1101	68-69	-0.22971777593592435
1101	70-71	-0.23818980968746217
1101	72-73	-0.25844397559703935
1101	74-75	-0.27918043152039473
1101	76-77	-0.27405082393446634
1101	78-79	-0.29456164211998015
1101	80-81	-0.29184916070311573
1101	82-83	-0.2610071507790934
1101	84-85	-0.258378422731397
1101	86-87	-0.29177044009297504
1101	88-89	-0.3066840127892476
1101	90-91	-0.33071701983293167
1101	92-93	-0.3048689314022397
1101	94-95	-0.2947550187547847
1101	96-97	-0.30925332313558584
1101	98-99	-0.3370457828255553
1101	100-101	-0.5616649505623386
1102	1	0.14936277881059112
1102	2	0.09911771111060119
1102	3	0.09280978564797238
1102	4	0.06361984415075028
1102	5	0.07849507056349125
1102	6	0.10830125248936895
1102	7	0.08076095141516504
1102	8	0.10943062886151012
1102	9	0.13226539554644745
1102	10-11	0.15066506674278202
1102	12-13	0.16099219853990832
1102	14-15	0.18582374734461382
1102	16-17	0.22195781398253445
1102	18-19	0.14777021271667934
1102	20-21	0.1445501826220763
1102	22-23	0.14557618413608253
1102	24-25	0.12866436013395344
1102	26-27	0.07682766538518848
1102	28-29	0.08191782342181142
1102	30-31	0.05059132649979148
1102	32-33	0.04798186128861914
1102	34-35	-0.009056259627165275
1102	36-37	-0.04871416484443358
1102	38-39	-0.09128517330366037
1102	40-41	-0.14282922845038826
1102	42-43	-0.11711012832338952
1102	44-45	-0.054423043004334204
1102	46-47	-0.04756474436545943
1102	48-49	-0.04633832336865851
1102	50-51	-0.045242242530896704
1102	52-53	-0.05993560972407863
1102	54-55	-0.07931062480787432
1102	56-57	-0.11037103540031268
1102	58-59	-0.11218880649992258
1102	60-61	-0.12671502749059727
1102	62-63	-0.15717404337902252
1102	64-65	-0.2089072901943041
1102	66-67	-0.2267795024549244
1102	68-69	-0.2208069328230451
1102	70-71	-0.25056924297395256
1102	72-73	-0.24729710794505877
1102	74-75	-0.2874780445090792
1102	76-77	-0.31476216084099207
1102	78-79	-0.280361563423952
1102	80-81	-0.2938219049230639
1102	82-83	-0.2907367491921349
1102	84-85	-0.26817423411114305
1102	86-87	-0.252638778073937
1102	88-89	-0.23794149422607802
1102	90-91	-0.2517110245159273
1102	92-93	-0.2107465730681426
1102	94-95	-0.2281076291325519
1102	96-97	-0.2534633529814556
1102	98-99	-0.2995750435056621
1102	100-101	-0.5418843120641306
1103	1	0.14933971811251467
1103	2	0.10689437628323617
1103	3	0.08448382413321553
1103	4	0.06479615239084069
1103	5	0.06845789146437653
1103	6	0.1178257494917716
1103	7	0.08517871064368165
1103	8	0.08713101078183172
1103	9	0.10347298410822248
1103	10-11	0.12282038318263488
1103	12-13	0.14838319207608208
1103	14-15	0.1922453892827818
1103	16-17	0.24098010165581485
1103	18-19	0.15821108942509454
1103	20-21	0.13635714848481229
1103	22-23	0.1796123892131547
1103	24-25	0.11574250216791881
1103	26-27	0.09414567500436277
1103	28-29	0.04433124966077884
1103	30-31	0.029349257656583916
1103	32-33	0.06105239639570925
1103	34-35	-0.01786111542803326
1103	36-37	-0.05512312260054841
1103	38-39	-0.11216308384086915
1103	40-41	-0.10378685007027855
1103	42-43	-0.08147335562524205
1103	44-45	-0.017284671799387752
1103	46-47	0.007467114377718076
1103	48-49	0.012237009564216805
1103	50-51	0.0060607713956244424
1103	52-53	7.486392118920548E-4
1103	54-55	-0.023248458460720656
1103	56-57	-0.08008788254505106
1103	58-59	-0.08449463999982498
1103	60-61	-0.13663982188639068
1103	62-63	-0.13936039549831492
1103	64-65	-0.17533239371130804
1103	66-67	-0.2167113830728269
1103	68-69	-0.2200208829450645
1103	70-71	-0.21243074710259435
1103	72-73	-0.20566908014728824
1103	74-75	-0.24867313453962225
1103	76-77	-0.23583139279632093
1103	78-79	-0.20667412212620917
1103	80-81	-0.2651730986633041
1103	82-83	-0.24353176731015935
1103	84-85	-0.1910958925165147
1103	86-87	-0.19350787711225337
1103	88-89	-0.2306955617388411
1103	90-91	-0.22741708702978514
1103	92-93	-0.2056945696775756
1103	94-95	-0.24839231729261257
1103	96-97	-0.2524885892681539
1103	98-99	-0.2967691071857814
1103	100-101	-0.6033736209039979
1104	1	0.13498193101481348
1104	2	0.0699761953684046
1104	3	0.07644249769141709
1104	4	0.05783723895015669
1104	5	0.0601904310882162
1104	6	0.09555947187767089
1104	7	0.07386380882365984
1104	8	0.062162346264983626
1104	9	0.1013040688968303
1104	10-11	0.1331077561263001
1104	12-13	0.13840862362172857
1104	14-15	0.15143209987862605
1104	16-17	0.19020324730552574
1104	18-19	0.10958824435160608
1104	20-21	0.11342655449201544
1104	22-23	0.1467631948980994
1104	24-25	0.13046333499293894
1104	26-27	0.09199026485655537
1104	28-29	0.07682344534169516
1104	30-31	0.06314937655184139
1104	32-33	0.06234005367617357
1104	34-35	0.007176595352355264
1104	36-37	-0.01778331620251805
1104	38-39	-0.05709181606275138
1104	40-41	-0.08797035600692737
1104	42-43	-0.04026693178581553
1104	44-45	0.022086534308392913
1104	46-47	0.045334982436699534
1104	48-49	0.023054972407464902
1104	50-51	0.028799149209419284
1104	52-53	0.02368288214010761
1104	54-55	-0.021051682593359544
1104	56-57	-0.05500827237996475
1104	58-59	-0.043264411359530186
1104	60-61	-0.06615538414904876
1104	62-63	-0.09188701845140201
1104	64-65	-0.16830170564402636
1104	66-67	-0.13807655775050165
1104	68-69	-0.14709968012466845
1104	70-71	-0.180223018291791
1104	72-73	-0.18240257729980414
1104	74-75	-0.17555246102966038
1104	76-77	-0.15919439964612536
1104	78-79	-0.14290902553975116
1104	80-81	-0.21172796194843357
1104	82-83	-0.2010073439913782
1104	84-85	-0.19351322885288624
1104	86-87	-0.1793044893472171
1104	88-89	-0.181771362628794
1104	90-91	-0.19451340883883717
1104	92-93	-0.14493251359491666
1104	94-95	-0.1675965884068873
1104	96-97	-0.1875811957805169
1104	98-99	-0.2422499960499067
1104	100-101	-0.5280149825837057
1105	1	0.17674705351388553
1105	2	0.1169274530518365
1105	3	0.08474594734365581
1105	4	0.08985750889398503
1105	5	0.07094224666206372
1105	6	0.12546540123176442
1105	7	0.1035581100392804
1105	8	0.10139492187542487
1105	9	0.1319522879920072
1105	10-11	0.14045600625724575
1105	12-13	0.15644772701469378
1105	14-15	0.15348558326256523
1105	16-17	0.18040178333921375
1105	18-19	0.1436956919021668
1105	20-21	0.13856072796049546
1105	22-23	0.14183870773013751
1105	24-25	0.11536073599491203
1105	26-27	0.12102390786581196
1105	28-29	0.11892671267717958
1105	30-31	0.11270116679986586
1105	32-33	0.10762527731716887
1105	34-35	0.04088593466934043
1105	36-37	0.003318655920317326
1105	38-39	-0.008495888563210485
1105	40-41	-0.08707678365420435
1105	42-43	-0.03871302876645899
1105	44-45	0.0482811974668067
1105	46-47	0.05264412334740598
1105	48-49	0.028499899005581142
1105	50-51	0.04679255184898068
1105	52-53	0.05297191813485114
1105	54-55	0.01378929830681841
1105	56-57	-3.1647953196767276E-4
1105	58-59	-0.008704527165065201
1105	60-61	-0.0335580446241579
1105	62-63	-0.058324530182545686
1105	64-65	-0.10212871189423112
1105	66-67	-0.10023822747120192
1105	68-69	-0.08680467181304152
1105	70-71	-0.11124337090600278
1105	72-73	-0.12539871090363164
1105	74-75	-0.13191774588544547
1105	76-77	-0.11764850143678274
1105	78-79	-0.12882555008351204
1105	80-81	-0.16144174372738007
1105	82-83	-0.13856134160290168
1105	84-85	-0.12853012253684426
1105	86-87	-0.1370243937462874
1105	88-89	-0.13588220218721148
1105	90-91	-0.16240834195776443
1105	92-93	-0.10401865949389588
1105	94-95	-0.13289736946755681
1105	96-97	-0.16633862112225017
1105	98-99	-0.20535325609803579
1105	100-101	-0.5519580668246746
1106	1	0.1350335634019686
1106	2	0.07181315439613911
1106	3	0.07437134636858644
1106	4	0.06753671907461722
1106	5	0.05814195850286552
1106	6	0.0886086465048308
1106	7	0.06286911498219894
1106	8	0.07280223629110338
1106	9	0.0698817669923315
1106	10-11	0.09911401054868207
1106	12-13	0.11420245576545085
1106	14-15	0.09153713509866179
1106	16-17	0.11375466942138956
1106	18-19	0.07615444207840483
1106	20-21	0.08777141137792199
1106	22-23	0.12422019494916725
1106	24-25	0.09288435641123982
1106	26-27	0.084728165299218
1106	28-29	0.04244442127830439
1106	30-31	0.053126996630389556
1106	32-33	0.05764033714804384
1106	34-35	-0.007317420948261599
1106	36-37	-0.03195657111539418
1106	38-39	-0.09564603648262704
1106	40-41	-0.08916025821319096
1106	42-43	-0.07287427723618833
1106	44-45	0.013649658562997047
1106	46-47	0.03922420765889001
1106	48-49	7.571280766498489E-4
1106	50-51	0.005361809636880821
1106	52-53	0.021721859829170853
1106	54-55	0.001614854923118969
1106	56-57	-0.04548633634961874
1106	58-59	-0.05994706801411809
1106	60-61	-0.0698588769844406
1106	62-63	-0.10493687138273344
1106	64-65	-0.12124983412920187
1106	66-67	-0.1294374367584581
1106	68-69	-0.1214056190847117
1106	70-71	-0.1476256558263387
1106	72-73	-0.13200139937643485
1106	74-75	-0.16474984049163055
1106	76-77	-0.16787265371456073
1106	78-79	-0.16501196967616494
1106	80-81	-0.16143269608642896
1106	82-83	-0.15136079441629846
1106	84-85	-0.12361090540070307
1106	86-87	-0.1647196046849615
1106	88-89	-0.16601530508226858
1106	90-91	-0.16528281365118147
1106	92-93	-0.14512178807049025
1106	94-95	-0.1910651191946826
1106	96-97	-0.18537857999600504
1106	98-99	-0.22048533778020385
1106	100-101	-0.5750229147462242
1107	1	0.1360296569901962
1107	2	0.09298221205837365
1107	3	0.08074022979268136
1107	4	0.049748882989277377
1107	5	0.05720460874691469
1107	6	0.09284659909261705
1107	7	0.06487458504053478
1107	8	0.047908638346200405
1107	9	0.08540705510607438
1107	10-11	0.08586660156171888
1107	12-13	0.13060322470546026
1107	14-15	0.13980491933879335
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2215	88-89	0.03851953876907288
2215	90-91	0.07603580374892971
2215	92-93	0.1158258766616811
2215	94-95	0.08395786777685998
2215	96-97	0.059825615375189045
2215	98-99	0.08829790671703108
2215	100-101	0.13684377316373997
2216	1	-0.3544268515793334
2216	2	-0.33563748046643127
2216	3	-0.2769576494471764
2216	4	-0.172883071004712
2216	5	-0.29598874195501423
2216	6	-0.219886472562969
2216	7	-0.169433290494041
2216	8	-0.20136796219203035
2216	9	-0.3157712441654823
2216	10-11	-0.296601642902246
2216	12-13	-0.34034174198649225
2216	14-15	-0.459702019427084
2216	16-17	-0.5339622937881714
2216	18-19	-0.4196697728631875
2216	20-21	-0.46760407270321735
2216	22-23	-0.4433522430381416
2216	24-25	-0.4746890565648272
2216	26-27	-0.3943005453066988
2216	28-29	-0.37820096845084805
2216	30-31	-0.37305567657559635
2216	32-33	-0.3721131640108979
2216	34-35	-0.36938743304548183
2216	36-37	-0.37674984894899666
2216	38-39	-0.3655986159564364
2216	40-41	-0.3782396948465987
2216	42-43	-0.35227182124028644
2216	44-45	-0.43456535414042463
2216	46-47	-0.47415989697934435
2216	48-49	-0.407103397641734
2216	50-51	-0.34777578450539437
2216	52-53	-0.3126988296357567
2216	54-55	-0.33783136571665295
2216	56-57	-0.3374896181297302
2216	58-59	-0.32775532469940316
2216	60-61	-0.2889760855236645
2216	62-63	-0.34587232475355734
2216	64-65	-0.30336566446069924
2216	66-67	-0.3516242424161149
2216	68-69	-0.37815299571818173
2216	70-71	-0.3376868913208071
2216	72-73	-0.3162624075634781
2216	74-75	-0.29654995515839033
2216	76-77	-0.21082133617014165
2216	78-79	-0.19278255359511576
2216	80-81	-0.11257127828309876
2216	82-83	-0.13637510115740525
2216	84-85	-0.12802263128571667
2216	86-87	-0.13830249900141212
2216	88-89	-0.14122797462598413
2216	90-91	-0.1934250379699094
2216	92-93	-0.1768998735292442
2216	94-95	-0.1552635007825387
2216	96-97	-0.1723509804258967
2216	98-99	-0.1508734837549781
2216	100-101	-0.011895270856669526
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	37683.0
3	13380.0
4	11165.0
5	18725.0
6	73156.0
7	66977.0
8	46843.0
9	60277.0
10	88799.0
11	88402.0
12	72880.0
13	47720.0
14	41957.0
15	37004.0
16	35725.0
17	34625.0
18	37710.0
19	33955.0
20	39709.0
21	50047.0
22	55653.0
23	67841.0
24	79009.0
25	90790.0
26	108759.0
27	143229.0
28	144345.0
29	159091.0
30	189491.0
31	239998.0
32	321022.0
33	455813.0
34	682096.0
35	971237.0
36	1771921.0
37	4911430.0
38	1.1519506E7
39	6789793.0
40	18161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.11663402293891	18.70849823224268	19.047308385689888	36.12755935912852
2	14.639989825905422	22.41370506881735	54.34856878714554	8.597736318131693
3	13.87555998143793	22.777006803665483	47.05582114047698	16.29161207441961
4	17.31736720926938	31.487781599171743	37.64266464087399	13.552186550684887
5	19.646783909285606	36.46572359242592	32.40668588906683	11.480806609221652
6	15.765151726522596	37.678414892201836	33.40011800732463	13.156315373950934
7	13.202563680562488	16.234182722271537	56.122996365735936	14.440257231430042
8	14.855848058219815	22.426657964524292	43.85220756606132	18.865286411194575
9	17.467944528891653	23.488241563086216	41.335117534788	17.70869637323413
10-11	18.979052899151995	32.83393076824001	32.942966137755754	15.244050194852242
12-13	18.96760027399885	23.621916503972955	40.455167979648195	16.955315242380003
14-15	18.619824415131774	28.258826745819643	38.03298556417284	15.088363274875743
16-17	20.299025856130935	28.721602225147137	35.66387774841544	15.315494170306494
18-19	19.794788561511204	29.105788809747434	33.00281686805378	18.096605760687584
20-21	21.425254263098108	28.915891884456705	32.33048980414131	17.328364048303875
22-23	21.038526996197604	30.810674150197354	31.937793764721533	16.213005088883506
24-25	20.71653013489771	30.053741659205365	31.207176830923434	18.022551374973496
26-27	20.598282137806194	30.624105851487617	31.179273923348777	17.59833808735742
28-29	21.495549249085567	29.86656455819973	31.079689362172676	17.558196830542023
30-31	20.56596708669589	31.63834176344775	30.13110817685944	17.664582972996918
32-33	20.33270255134677	30.4453126193158	31.208663733067755	18.01332109626967
34-35	21.533926733237635	30.84925661397592	29.44235751287687	18.174459139909573
36-37	21.525453347079775	30.847908489624114	29.814855977573007	17.811782185723104
38-39	22.194918607523416	28.882615702379876	30.925255455931993	17.99721023416472
40-41	21.87862875478022	30.997004071354716	28.799524924489827	18.32484224937524
42-43	22.189198862472136	30.454793795419842	29.38033319627439	17.97567414583363
44-45	22.440317828415473	29.570666614332776	29.691964598013044	18.29705095923871
46-47	20.740194321815082	30.741189240263616	29.655619850665598	18.862996587255708
48-49	21.197011952295586	29.889921298053135	30.126769464782182	18.786297284869097
50-51	20.751520075842755	30.080350414513298	30.970082368688338	18.19804714095561
52-53	23.090236135962872	28.540592730363308	28.79618427496303	19.57298685871079
54-55	21.983787675023223	28.89256598356677	30.389347781714772	18.734298559695237
56-57	22.398956431121054	29.41750772022734	29.5398710175894	18.6436648310622
58-59	21.88907385978872	29.973052148243617	29.01071683714885	19.127157154818818
60-61	22.52889523196198	29.437483471785995	28.772628050552957	19.26099324569907
62-63	23.04905939197425	28.707275518199456	29.086701040920417	19.156964048905877
64-65	22.541862943632633	29.13070017628553	29.69684031249209	18.630596567589745
66-67	22.963627306998955	28.861335999744142	28.9107479434363	19.264288749820597
68-69	23.187505387109127	28.336138936659932	29.38559311946519	19.09076255676575
70-71	22.197961882964137	28.32203987126355	29.876273399502786	19.603724846269532
72-73	22.999492545374228	27.660081488410597	29.687987627411257	19.652438338803915
74-75	23.66389739972093	27.72996115658199	29.51485064806812	19.09129079562896
76-77	21.89939698223605	27.977421427221255	29.809077530971763	20.314104059570937
78-79	21.96710197815887	28.61348484001765	29.57576887289629	19.84364430892719
80-81	22.026097016802424	28.10802063617143	30.509125619282855	19.35675672774329
82-83	22.426756755774097	28.935127191388236	28.90247088668604	19.73564516615163
84-85	22.412063828559635	28.954164042039242	28.776133790186325	19.85763833921479
86-87	22.258651206902766	29.730699981682147	29.55031392306804	18.460334888347056
88-89	23.44365526813633	27.519282955567796	30.20495028032748	18.83211149596839
90-91	23.599330733206987	27.7941393638829	28.815938398716195	19.790591504193923
92-93	23.124083598922304	27.89599758213064	29.070192408489227	19.90972641045783
94-95	23.819977341603813	28.257705387701115	28.03872767942072	19.883589591274355
96-97	23.068234072482745	28.011305343194557	29.58722648578826	19.333234098534437
98-99	24.975709171539407	26.91844924714993	28.737697214987147	19.368144366323524
100-101	24.98776422728779	27.056396693016726	28.231630399000647	19.724208680694836
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	55.0
1	108.5
2	235.0
3	469.0
4	839.5
5	1421.0
6	2209.0
7	3176.5
8	4348.5
9	5545.0
10	6724.0
11	8164.0
12	9517.0
13	10871.5
14	12379.0
15	14800.0
16	19444.5
17	23005.0
18	28308.5
19	34696.5
20	43956.5
21	60153.5
22	82133.0
23	115808.5
24	158031.0
25	222452.5
26	277746.0
27	410557.5
28	542606.5
29	700589.5
30	878117.5
31	899572.5
32	977966.5
33	1157025.5
34	1513877.5
35	1634995.5
36	1412951.0
37	1435064.0
38	1465682.0
39	1470050.0
40	1507838.0
41	1445475.0
42	1228482.0
43	994413.0
44	858932.0
45	724464.5
46	642391.0
47	619018.0
48	632013.0
49	608467.5
50	532338.0
51	474853.5
52	481185.0
53	468271.5
54	450883.0
55	395906.5
56	327814.5
57	320648.5
58	303467.0
59	230523.5
60	148414.0
61	102574.0
62	73670.5
63	54216.0
64	42574.0
65	33308.0
66	23718.5
67	14618.0
68	7533.5
69	5171.0
70	3926.5
71	5704.0
72	17605.0
73	40598.5
74	48613.5
75	34559.0
76	18567.5
77	38291.0
78	37911.5
79	6997.5
80	2001.0
81	555.5
82	303.5
83	248.0
84	231.0
85	247.0
86	224.0
87	126.0
88	68.5
89	46.0
90	39.0
91	26.0
92	19.0
93	17.0
94	18.0
95	19.5
96	18.0
97	16.0
98	18.5
99	22.5
100	43.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2441164874849288
2	0.4116951473169408
3	0.4133373149998631
4	0.44735075528248586
5	0.4417161306456005
6	0.05189856839395731
7	0.016199124330100116
8	0.01681957372159438
9	0.02546877311932685
10-11	0.01684317777453166
12-13	0.1536994767048904
14-15	0.3178622928761215
16-17	0.20487474947669815
18-19	0.3283880144823679
20-21	0.17647738778936714
22-23	0.1623705941517789
24-25	0.12288944360661297
26-27	0.1046047325991259
28-29	0.10086349020856676
30-31	0.08446541743228098
32-33	0.09224632488267774
34-35	0.07835702573286875
36-37	0.11204675328949454
38-39	0.11492813375162414
40-41	0.12778391258353644
42-43	0.16671373989223873
44-45	0.1724613267824668
46-47	0.20411941978270512
48-49	0.22425030493064385
50-51	0.22350340525555704
52-53	0.24147620556351576
54-55	0.2912858152725237
56-57	0.2933933199990666
58-59	0.3339046188545668
60-61	0.3831831373724858
62-63	0.36494563447087336
64-65	0.4592708020158131
66-67	0.4167481006493003
68-69	0.49508658034057546
70-71	0.5060101988391932
72-73	0.5517683414618948
74-75	0.628719240041214
76-77	0.6724187720470285
78-79	0.6502764843880771
80-81	0.7400072916291531
82-83	0.7644037663436148
84-85	0.7926965957965094
86-87	0.8620453033262426
88-89	0.8933864276156089
90-91	0.8773053235501952
92-93	0.92623652528918
94-95	0.9622175319845032
96-97	0.9820129023799764
98-99	0.9931691894004044
100-101	1.0095116240519095
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.9655924E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.761169029760563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.8631179505331	9.170573951723734
2	10.072328820978075	2.570693812156603
3	4.344611012133575	1.6632694648318704
4	2.62604027780821	1.3404537545627984
5	1.7611009323443998	1.1236853338057904
6	1.2554274011263324	0.9612432762219687
7	0.9218192073293915	0.8234443503727185
8	0.6964773629472003	0.7110292283176897
9	0.5925750267259925	0.6805755070978746
>10	4.537005320124872	11.775828164753479
>50	0.6467323181504315	5.780205216318173
>100	0.5381142657176563	13.97938296364812
>500	0.07262385131588746	6.425655966979432
>1k	0.05852025715649328	14.963910996301138
>5k	0.007453406578450867	6.612951734167666
>10k+	0.006052589030018612	21.417096278741205
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA	234927	0.792175620628108	No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT	169804	0.5725803721374523	No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT	143447	0.48370436881346207	No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT	140995	0.47543620627028854	No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA	120478	0.4062527271111161	No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA	119538	0.4030830400023955	No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA	109619	0.36963609699026745	No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC	105062	0.3542698585280971	No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA	100598	0.3392172167692364	No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG	100520	0.33895420017936384	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	91333	0.30797556670296294	No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG	87143	0.29384685501621866	No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC	79205	0.2670798589853413	No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT	76832	0.2590780850396029	No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA	75346	0.25406728180177424	No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT	74906	0.25258359847428796	No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT	71138	0.23987787397890553	No Hit
CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA	70413	0.23743316849611562	No Hit
CTCAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCT	64529	0.21759227599854924	No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA	59902	0.2019899970070061	No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA	58407	0.1969488457011152	No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT	57167	0.19276755632365392	No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG	55617	0.1875409446018273	No Hit
CAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTGTGTAGAAAT	55593	0.18746001642032803	No Hit
CAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTG	52100	0.17568159400462452	No Hit
GTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCTTCTT	51851	0.17484196412156977	No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC	50986	0.17192517758003426	No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT	50601	0.17062695466848377	No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG	50323	0.16968953656611743	No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG	49428	0.16667158979770788	No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA	48106	0.1622137958001241	No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT	47145	0.1589732965325916	No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG	45406	0.15310937538145836	No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA	41349	0.13942914070052245	No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC	41036	0.13837370233346968	No Hit
GTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTG	40817	0.13763523267728903	No Hit
GTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAAT	40520	0.1366337464312358	No Hit
CGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAG	40464	0.13644491400773756	No Hit
GTTTATTTTACACTGGGCGAATTGCTTCTGTACAATAGAAAGCAGTCTGT	40357	0.13608410919855338	No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT	39997	0.13487018647606464	No Hit
GTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCT	39876	0.13446217356100587	No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC	39351	0.13269186959070978	No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA	39193	0.1321590923958397	No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA	39006	0.13152852698165804	No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA	38482	0.12976159501892437	No Hit
CAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCA	36224	0.12214760194286982	No Hit
GAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGT	36052	0.12156761664212519	No Hit
GTTCTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTT	35252	0.11887001059215016	No Hit
TGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACA	32878	0.11086486463884923	No Hit
CAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGT	31488	0.1061777741270176	No Hit
TGTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAA	31368	0.10577313321952134	No Hit
TTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGAT	30596	0.10316994338129541	No Hit
TTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATG	30083	0.10144010350174892	No Hit
GTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGT	29819	0.10054989350525717	No Hit
GTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATT	29724	0.10022955278682262	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3604052937281603E-5	0.0	0.0	0.0	0.0
2	2.3604052937281603E-5	3.3720075624688003E-6	0.0	0.0	0.0
3	2.6976060499750402E-5	3.3720075624688003E-6	0.0	0.0	0.0
4	2.6976060499750402E-5	3.3720075624688003E-6	0.0	0.0	0.0
5	2.6976060499750402E-5	3.3720075624688003E-6	0.0	0.0	0.0
6	3.0348068062219206E-5	3.3720075624688003E-6	0.0	0.0	0.0
7	3.3720075624688006E-5	3.3720075624688003E-6	0.0	0.0	0.0
8	3.3720075624688006E-5	3.3720075624688003E-6	0.0	0.0	0.0
9	3.3720075624688006E-5	3.3720075624688003E-6	0.0	0.0	0.0
10-11	3.7092083187156806E-5	3.3720075624688003E-6	0.0	0.0	0.0
12-13	5.0580113437032005E-5	3.3720075624688003E-6	0.0	0.0	0.0
14-15	5.9010132343204E-5	3.3720075624688003E-6	0.0	0.0	0.0
16-17	7.249816259307921E-5	3.3720075624688003E-6	0.0	0.0	0.0
18-19	9.104420418665762E-5	3.3720075624688003E-6	0.0	0.0	0.0
20-21	1.5005433652986162E-4	3.3720075624688003E-6	0.0	0.0	0.0
22-23	3.5406079405922404E-4	3.3720075624688003E-6	0.0	0.0	0.0
24-25	7.401556599619017E-4	3.3720075624688003E-6	0.0	0.0	0.0
26-27	0.0013589190476749266	3.3720075624688003E-6	0.0	3.3720075624688003E-6	0.0
28-29	0.0025897018079760387	3.3720075624688003E-6	0.0	3.3720075624688003E-6	0.0
30-31	0.005209751684014297	3.3720075624688003E-6	0.0	3.3720075624688003E-6	0.0
32-33	0.010475141492809328	3.3720075624688003E-6	0.0	3.3720075624688003E-6	0.0
34-35	0.018232444890268804	3.3720075624688003E-6	0.0	3.3720075624688003E-6	0.0
36-37	0.02920495749854228	3.3720075624688003E-6	0.0	3.3720075624688003E-6	0.0
38-39	0.04532146764336191	3.3720075624688003E-6	0.0	6.744015124937601E-6	0.0
40-41	0.0647003951048701	3.3720075624688003E-6	0.0	6.744015124937601E-6	0.0
42-43	0.08714279143688121	3.3720075624688003E-6	0.0	6.744015124937601E-6	0.0
44-45	0.1154069588254947	3.3720075624688003E-6	0.0	6.744015124937601E-6	0.0
46-47	0.14652215860817555	3.3720075624688003E-6	0.0	6.744015124937601E-6	0.0
48-49	0.18311518467608698	3.3720075624688003E-6	0.0	6.744015124937601E-6	0.0
50-51	0.2218410055272599	3.3720075624688003E-6	0.0	6.744015124937601E-6	0.0
52-53	0.2695077044303189	3.3720075624688003E-6	0.0	6.744015124937601E-6	0.0
54-55	0.3329891188013565	3.3720075624688003E-6	0.0	6.744015124937601E-6	0.0
56-57	0.3975866676755713	3.3720075624688003E-6	0.0	1.1802026468640801E-5	0.0
58-59	0.4613783067423561	3.3720075624688003E-6	0.0	1.6860037812344003E-5	0.0
60-61	0.5330149214032245	3.3720075624688003E-6	0.0	1.6860037812344003E-5	0.0
62-63	0.6258007674958973	3.3720075624688003E-6	0.0	1.6860037812344003E-5	0.0
64-65	0.721916471056508	3.3720075624688003E-6	0.0	1.8546041593578403E-5	0.0
66-67	0.8265346916858838	3.3720075624688003E-6	0.0	2.0232045374812803E-5	0.0
68-69	0.9461600319720269	3.3720075624688003E-6	0.0	2.0232045374812803E-5	0.0
70-71	1.061008923545933	3.3720075624688003E-6	0.0	2.1918049156047203E-5	0.0
72-73	1.1892969512600584	3.3720075624688003E-6	0.0	3.3720075624688006E-5	0.0
74-75	1.3507419293359397	3.3720075624688003E-6	0.0	4.3836098312094405E-5	0.0
76-77	1.5172617787933365	3.3720075624688003E-6	0.0	4.7208105874563205E-5	0.0
78-79	1.6956460368592798	3.3720075624688003E-6	0.0	5.732412856196961E-5	0.0
80-81	1.8975298156280682	3.3720075624688003E-6	0.0	5.9010132343204015E-5	0.0
82-83	2.1218981408233986	3.3720075624688003E-6	0.0	6.069613612443841E-5	0.0
84-85	2.4308128116325087	3.3720075624688003E-6	0.0	6.912615503061041E-5	0.0
86-87	2.7340776837707033	3.3720075624688003E-6	0.0	7.418416637431361E-5	0.0
88-89	3.0844663615943984	3.3720075624688003E-6	0.0	7.418416637431361E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	20420	0.0	79.50168	1
GCGTGTT	24910	0.0	64.77128	2
GTAAACG	7245	0.0	56.199116	1
TAAACGC	8040	0.0	50.5241	2
CGAACGT	5810	0.0	48.00086	1
CGTGTTT	36270	0.0	44.52334	3
TCGAACG	2760	0.0	40.796955	2
CTTACGG	11620	0.0	37.983646	1
TTACGGT	12000	0.0	36.780952	2
TAGATAT	29510	0.0	36.610474	3
GTAGATA	29845	0.0	35.754166	2
GTCGAAC	3190	0.0	35.595432	1
GATCGGG	815	0.0	35.559795	1
CTCAGTT	43615	0.0	34.825214	1
GTTTTTT	208175	0.0	34.621353	1
TACGGTC	12880	0.0	34.083187	3
GATATAG	31480	0.0	33.874393	5
GTCGCGA	70	2.0775644E-4	33.864307	7
CCAAGAC	15970	0.0	33.527878	1
ACGGTCT	13585	0.0	33.188732	4
>>END_MODULE