FastQCFastQC Report
Fri 27 May 2016
SRR1233127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233127_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29655924
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC3382611.140618650088259No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA2071940.6986597348981607No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT1957030.6599119959978317No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA1467260.49476118161079724No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT1255490.42335217746039544No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC1166560.39336491420736036No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT1002190.3379392259030607No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC994160.33523150383039824No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA950000.32034071843453604No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT859740.28990497817569266No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT755100.2546202910420191No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG680830.22957639087556334No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC646970.218158773269044No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG638490.21529931085607046No Hit
AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA617590.20825181505051063No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG580590.1957753870693761No Hit
CTATATTCATAGCACTTATACTAAATAACTCTATAACCATCAACTCAATC577120.19460530044519941No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC556110.18752071255645247No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT544560.183626043821801No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA542090.18279315795387122No Hit
CTGGTATCTTTCAGAGTGCCAAGTGATTTCAGAATGCAAAGAATTCTTTG539770.18201085219937846No Hit
CTAACTTTGTGTCCGTCTCCATCCATGATTGTCTGTCTCACTGAGCCAAC523720.17659878006161603No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT521570.17587379843568524No Hit
CTTTTGTATTGGTATTGTATGATGAGATCAAAAAATATGTGTAATACCCA520510.17551636563406353No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA483000.16286796526724306No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT458630.1546503828375066No Hit
CTAACACTAGCATTATGTATGTGACATATTTCTTTACCAATTTTTACAGC458530.1546166627618819No Hit
CCAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACC456770.1540231894308874No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA439230.14810868816631712No Hit
CTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCA434420.14648675252876964No Hit
CTGCAGTATAGATTTGTGGTGCTAACTTTGTGTCCGTCTCCATCCATGAT423470.1427944042478663No Hit
ATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACT404830.13650898215142446No Hit
CCTAACACTAGCATTATGTATGTGACATATTTCTTTACCAATTTTTACAG395150.13324487883095465No Hit
GGAAGATTGCAAAAGATGAAGGAGCCAACGCTTTCTTCAAAGGTGCTTGG393000.13251989720502386No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC386830.1304393685389806No Hit
CCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTT375720.12669306813707779No Hit
CTTTTGTTTGATGGTTGCGTTCTTGATCTTGTTTGCCATGTAACAGAAAT374360.12623447510858202No Hit
ATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTG369380.12455521534247255No Hit
CTCAGCGTCAGTTCAGTTGCTCACAGCTTCTCACAGCGCATGTTTGACTG358450.12086961107669417No Hit
CACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGG357890.1206807786531959No Hit
GTCCGTCTCCATCCATGATTGTCTGTCTCACTGAGCCAACTTAACTCTGA356010.12004684123145178No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG354050.11938592774920788No Hit
CTCACTTCTATGAAATAAAACTCCAGCAATACTAACTATAATCTCACTGA332040.11196413910421404No Hit
CTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGA331000.1116134503177173No Hit
CGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTAT330140.11132345766734497No Hit
CCGCAAACAAAGATCACCCAGCTACTACCATCATTCAAGTAGCACAACTA329270.1110300930094102No Hit
CACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCT327440.11041301562547841No Hit
TCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCA326390.11005895483141917No Hit
GGACACTTGACTGCTGGAGGAAGATTGCAAAAGATGAAGGAGCCAACGCT320390.1080357502939379No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCC313000.10554383670527345No Hit
TAACAATTAAACCTAAACCTCCATAAATAGGTGAAGGCTTTAATGCTAAC310030.10454235045922022No Hit
TAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCA308130.10390166902235114No Hit
GTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCAT305140.10289343876117299No Hit
GTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATC302470.10199311274199381No Hit
CTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAATAGA296870.10010478850701128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT2750.058.8687251
CCCGAAA138200.053.29921
TACGCAT112750.047.989679
CGGCAAT125200.047.4242551
CTACGCA116950.046.144138
CTCTACG120150.044.9170536
CTCGTAC47950.043.3941571
TCCGCTC27550.043.1038932
CCGCAAA81650.042.453511
TCGTACC48800.042.1469542
TCCACTC797100.041.8929182
TAACGGC33400.041.6736689
CCGCTCA25350.041.223313
CTCCACT832650.040.5149961
TCTACGC147600.040.297187
CGCAAAC86050.039.5791472
GGCAATC153850.038.685812
CCACTCA863300.038.6309473
CGTACCA54350.037.6682853
CTCCGCT38350.037.12311
Produced by FastQC (version 0.11.5)