FastQCFastQC Report
Fri 27 May 2016
SRR1233126_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233126_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16803769
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA997310.5935037550206742No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT961790.5723656401132389No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA952320.5667299996804288No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT791750.47117405624892844No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT532440.3168574859604414No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA492130.29286882008435133No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC462980.2755215213920163No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA438120.26072722137515697No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG418850.24925955599603872No Hit
GTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCTTCTT413070.24581985148688962No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT393920.23442359865813436No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG381680.22713951852111275No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA374750.22301544373765195No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG374380.22279525504069952No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG353460.2103456670940906No Hit
CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT348020.2071082981443032No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT342070.20356742585547327No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT333480.19845547745865824No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT331530.19729502351526015No Hit
CTCAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCT327770.1950574302705542No Hit
GATTTATTTACCTGAAATCATGTCTGTGCAATACCTGTTACCAGCACAGA315720.18788642000494055No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT310590.18483353347692413No Hit
GTTCTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTT305910.1820484440127688No Hit
GTTTATTTTACACTGGGCGAATTGCTTCTGTACAATAGAAAGCAGTCTGT305350.18171518544440834No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA304890.18144143733468365No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA285600.16996186986383827No Hit
CAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCA278750.16588540344728614No Hit
CAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTG271470.16155304205860008No Hit
CAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGT254540.15147792141155952No Hit
CGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAG250440.14903799260749181No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT249500.1484785942963153No Hit
AGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATAATAAAT248710.1480084616730925No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC239820.1427179819003701No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA230250.13702283100892426No Hit
CCCAATTCAGGTTAAGATAAGTAGGTTGGCTACTAGGATTCAGTACAAAA227690.13549936326784784No Hit
GGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGG227150.13517800679121453No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA215980.1285306885615959No Hit
GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC212080.1262097806747998No Hit
GTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGTGGGTCACCTGA211980.126150270216164No Hit
CCAATACAAAAGCACCACCCATGCCTCTCAGTACATTGGACCAAGCACCT210430.12522785810730913No Hit
ATTTTTTTTAACTCACAATCACAACACAGTCCAAGGAGGCCAAACACTTC207840.12368653722864198No Hit
GTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAAT206470.12287124394533155No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT202920.12075862266376074No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC178750.10637494481148843No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC176980.10532160969363481No Hit
GTTGGCTTTTTATTGCTGGCATAGTGCGGGCATAGGGGTCAGGCAGAGTA176150.10482767288695768No Hit
ATTTACCTGAAATCATGTCTGTGCAATACCTGTTACCAGCACAGAAACTG176120.10480981974936693No Hit
AAAATGTTCTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTG174510.1038517013653306No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG173210.10307806540306523No Hit
ATTTATTTACCTGAAATCATGTCTGTGCAATACCTGTTACCAGCACAGAA169930.10112612235981105No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT38550.060.8759041
CGAACGT27400.057.3878941
TCGAACG11100.055.2092932
GCGTGTT43400.053.5259782
GTAAACG32050.051.4332961
TAAACGC36100.045.663352
GTCGAAC14200.043.15631
TTATCGT25950.039.1744043
CGGAGTT24650.038.736651
TATCGTA26850.038.0382164
ATCAATA256900.037.333464
ATCGTAG28400.035.6186035
TCATCAA269850.035.5258262
GAACGTT45450.034.8061332
CCAAGAC110300.034.5415421
AGTTTCG22700.034.1104744
GACGGAG33900.034.0525361
ACGGAGT34200.034.031842
CATCAAT291850.032.976593
CTTACGG28000.032.5751231
Produced by FastQC (version 0.11.5)