##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233126_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16803769
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67672044289588	34.0	31.0	34.0	31.0	34.0
2	32.91162387438199	34.0	33.0	34.0	31.0	34.0
3	32.985070432710664	34.0	33.0	34.0	31.0	34.0
4	36.22471774040693	37.0	37.0	37.0	35.0	37.0
5	36.205318640121746	37.0	37.0	37.0	35.0	37.0
6	36.232198919182956	37.0	37.0	37.0	35.0	37.0
7	36.27664626906023	37.0	37.0	37.0	35.0	37.0
8	36.30541213700331	37.0	37.0	37.0	35.0	37.0
9	38.19124388106025	39.0	39.0	39.0	37.0	39.0
10-11	38.137032828765975	39.0	39.0	39.0	37.0	39.0
12-13	38.06772718668056	39.0	39.0	39.0	37.0	39.0
14-15	39.55327170945994	41.0	40.0	41.0	38.0	41.0
16-17	39.52713180001463	41.0	40.0	41.0	37.5	41.0
18-19	39.47059921497373	41.0	40.0	41.0	37.0	41.0
20-21	39.439616909753994	41.0	40.0	41.0	37.0	41.0
22-23	39.39297523073544	41.0	40.0	41.0	37.0	41.0
24-25	39.36976841326491	41.0	40.0	41.0	37.0	41.0
26-27	39.30477281614618	41.0	40.0	41.0	37.0	41.0
28-29	39.25318557997316	41.0	40.0	41.0	37.0	41.0
30-31	39.174704020270696	41.0	39.5	41.0	37.0	41.0
32-33	39.11004602598381	41.0	39.0	41.0	37.0	41.0
34-35	39.08974742511636	41.0	39.0	41.0	36.5	41.0
36-37	39.004860486953845	41.0	39.0	41.0	36.0	41.0
38-39	38.960385762265595	41.0	39.0	41.0	36.0	41.0
40-41	38.91514894664405	41.0	39.0	41.0	36.0	41.0
42-43	38.92851907212007	41.0	39.0	41.0	36.0	41.0
44-45	39.020339573818234	41.0	39.0	41.0	36.0	41.0
46-47	38.99644877289137	41.0	40.0	41.0	36.0	41.0
48-49	38.9509871267571	41.0	39.0	41.0	36.0	41.0
50-51	38.89826624610229	41.0	39.0	41.0	36.0	41.0
52-53	38.79373618501897	41.0	39.0	41.0	35.0	41.0
54-55	38.68316146812063	41.0	39.0	41.0	35.0	41.0
56-57	38.578796667580946	41.0	39.0	41.0	35.0	41.0
58-59	38.433637358380736	40.0	38.5	41.0	35.0	41.0
60-61	38.280745557737674	40.0	38.0	41.0	34.5	41.0
62-63	38.06682640662342	40.0	37.5	41.0	34.0	41.0
64-65	37.78774624907067	40.0	37.0	41.0	34.0	41.0
66-67	37.50210925894066	39.0	36.5	41.0	34.0	41.0
68-69	37.15307937760868	39.0	36.0	41.0	33.0	41.0
70-71	36.72632755782349	38.5	35.5	40.5	33.0	41.0
72-73	36.340616947305094	37.0	35.0	40.0	32.5	41.0
74-75	35.880561676371535	37.0	35.0	39.0	32.0	41.0
76-77	35.61577042626568	36.5	35.0	39.0	32.0	41.0
78-79	35.27847478741228	36.0	35.0	39.0	32.0	39.5
80-81	34.90980904343543	36.0	35.0	37.0	32.0	39.0
82-83	34.52959205164032	35.0	35.0	37.0	32.0	39.0
84-85	34.19135144621424	35.0	35.0	36.5	32.0	38.0
86-87	33.93888246142875	35.0	35.0	36.0	32.0	37.0
88-89	33.70668508951772	35.0	35.0	36.0	31.0	37.0
90-91	33.50972180705412	35.0	35.0	35.0	31.0	36.0
92-93	33.32550941398921	35.0	34.0	35.0	31.0	36.0
94-95	33.198347376710544	35.0	34.0	35.0	31.0	36.0
96-97	33.11540714467094	35.0	34.0	35.0	31.0	36.0
98-99	32.99933009076714	35.0	34.0	35.0	31.0	36.0
100-101	32.30853468052316	34.5	33.0	35.0	28.5	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.11440893810463848
1101	2	0.08129119684848263
1101	3	0.07606379704819233
1101	4	0.05166256039282757
1101	5	0.04828535497286168
1101	6	0.12243883595604643
1101	7	0.07669013299184257
1101	8	0.10897034388409566
1101	9	0.14191339219772203
1101	10-11	0.16948860173627622
1101	12-13	0.17035575540700876
1101	14-15	0.22167376155920948
1101	16-17	0.2600399349221547
1101	18-19	0.19516636765360573
1101	20-21	0.19704831250659538
1101	22-23	0.2238484581905169
1101	24-25	0.2095952622091417
1101	26-27	0.18074206463366949
1101	28-29	0.15707486972198126
1101	30-31	0.11198503939529303
1101	32-33	0.10625735834489802
1101	34-35	0.08841495204242733
1101	36-37	-0.0014054693260874274
1101	38-39	-0.026380919662187807
1101	40-41	-0.04740487692838258
1101	42-43	9.701583764822885E-4
1101	44-45	0.026018309719169963
1101	46-47	0.006874181707139826
1101	48-49	-0.011544927039061292
1101	50-51	0.029007467808341403
1101	52-53	0.06055049457011563
1101	54-55	0.01565719460340631
1101	56-57	-0.002125267320771229
1101	58-59	-0.0429733989294121
1101	60-61	-0.04397939165165354
1101	62-63	-0.03318276876909465
1101	64-65	-0.09771488487302094
1101	66-67	-0.07454385432969701
1101	68-69	-0.07621146504108367
1101	70-71	-0.09518139039918339
1101	72-73	-0.12851356902795175
1101	74-75	-0.17156803607026916
1101	76-77	-0.16660480950476142
1101	78-79	-0.17030509429391572
1101	80-81	-0.1767028573310867
1101	82-83	-0.15389768024735417
1101	84-85	-0.130572522838861
1101	86-87	-0.18075874200941655
1101	88-89	-0.17545706781475445
1101	90-91	-0.20152690950602903
1101	92-93	-0.17769794172002662
1101	94-95	-0.15180727279405204
1101	96-97	-0.17553413539024376
1101	98-99	-0.17489949464968646
1101	100-101	-0.4062275340915882
1102	1	0.1527350777364589
1102	2	0.09087019316085332
1102	3	0.10326961201673157
1102	4	0.07423821926984431
1102	5	0.0822243557581146
1102	6	0.09650477646965783
1102	7	0.05191552236959751
1102	8	0.07846568613494043
1102	9	0.08487579941309065
1102	10-11	0.12503416383772503
1102	12-13	0.16205231021869793
1102	14-15	0.19241883069951626
1102	16-17	0.2121462968603467
1102	18-19	0.15090783758224546
1102	20-21	0.13555805172985913
1102	22-23	0.14942696598296834
1102	24-25	0.14982564496881423
1102	26-27	0.1229307683861336
1102	28-29	0.1239000375252246
1102	30-31	0.10867149578378132
1102	32-33	0.1145477014026497
1102	34-35	0.02439934482888617
1102	36-37	-0.02273746786823949
1102	38-39	-0.07263934433213137
1102	40-41	-0.11341615776477454
1102	42-43	-0.061399694563881724
1102	44-45	0.02870056549617317
1102	46-47	0.031797083112167
1102	48-49	0.015282319573934444
1102	50-51	0.022035975780923422
1102	52-53	-0.012215217506692966
1102	54-55	-0.0147411888707083
1102	56-57	-0.059661202461192886
1102	58-59	-0.0740064183548057
1102	60-61	-0.07707714873924942
1102	62-63	-0.07845203066337092
1102	64-65	-0.1505164608375935
1102	66-67	-0.14588698385758647
1102	68-69	-0.17688551038878586
1102	70-71	-0.18957392946870044
1102	72-73	-0.17403122309201535
1102	74-75	-0.20230521456571182
1102	76-77	-0.2137346451349913
1102	78-79	-0.17354906901334743
1102	80-81	-0.20152273641541996
1102	82-83	-0.21639693360159384
1102	84-85	-0.19902373753563296
1102	86-87	-0.20252113028374907
1102	88-89	-0.17603458698348362
1102	90-91	-0.20247696653663638
1102	92-93	-0.16681057238321273
1102	94-95	-0.20790299160067605
1102	96-97	-0.2366513691712413
1102	98-99	-0.2613497635076172
1102	100-101	-0.4820102230085723
1103	1	0.13223370212324426
1103	2	0.1023897719892588
1103	3	0.09090908320487046
1103	4	0.06706499956753476
1103	5	0.06763003565757941
1103	6	0.11491713489865418
1103	7	0.07669902165004316
1103	8	0.08129774566641856
1103	9	0.09315363091822348
1103	10-11	0.12748344080323193
1103	12-13	0.15103715921296867
1103	14-15	0.18320470853631576
1103	16-17	0.2315711590549796
1103	18-19	0.17138448744830015
1103	20-21	0.15602836909496887
1103	22-23	0.17668230294901122
1103	24-25	0.13801644928650347
1103	26-27	0.11341039650569229
1103	28-29	0.08350764238358721
1103	30-31	0.09215749757683511
1103	32-33	0.11722590130307964
1103	34-35	0.056040911922195846
1103	36-37	0.021378557054056557
1103	38-39	-0.04837886573299954
1103	40-41	-0.07367040933058178
1103	42-43	-0.04913381817020479
1103	44-45	0.009075028685977315
1103	46-47	-0.004959455715770389
1103	48-49	0.02942815571764612
1103	50-51	0.049980679222940694
1103	52-53	0.029141026549503124
1103	54-55	7.152925172633218E-4
1103	56-57	-0.02123099925950811
1103	58-59	-0.018453855179167533
1103	60-61	-0.056224663000229214
1103	62-63	-0.06502440002325471
1103	64-65	-0.10900113290480817
1103	66-67	-0.14588030000265206
1103	68-69	-0.14504314132537388
1103	70-71	-0.13324883369393348
1103	72-73	-0.12971079595018864
1103	74-75	-0.14049591374816828
1103	76-77	-0.1390709436576003
1103	78-79	-0.1341767791674897
1103	80-81	-0.1666680605108084
1103	82-83	-0.18323207080621273
1103	84-85	-0.141276285816744
1103	86-87	-0.14656988419316264
1103	88-89	-0.18064935436918717
1103	90-91	-0.1763720817796326
1103	92-93	-0.13168440371028112
1103	94-95	-0.17177051979240332
1103	96-97	-0.18382737325912757
1103	98-99	-0.2571317091061118
1103	100-101	-0.593488087175718
1104	1	0.12634092777285133
1104	2	0.0726959368793274
1104	3	0.08825465022107437
1104	4	0.05645680644164486
1104	5	0.06133567673341389
1104	6	0.10987931104990878
1104	7	0.08670428854595258
1104	8	0.08116485454778655
1104	9	0.12071800332915217
1104	10-11	0.13746799515750752
1104	12-13	0.1220308971569608
1104	14-15	0.15836185457387586
1104	16-17	0.20536535322548133
1104	18-19	0.15088173720969422
1104	20-21	0.17541285568010068
1104	22-23	0.17180275020222524
1104	24-25	0.1422439885563378
1104	26-27	0.1436997409203684
1104	28-29	0.12537828614093627
1104	30-31	0.08992665909902797
1104	32-33	0.06879495552895776
1104	34-35	0.049018901474902066
1104	36-37	0.004755247228970916
1104	38-39	-0.03322475404235803
1104	40-41	-0.08574274463524745
1104	42-43	-0.02439431595811925
1104	44-45	0.04783303996069321
1104	46-47	0.05549610670488647
1104	48-49	0.017473071646477933
1104	50-51	0.03631837858671361
1104	52-53	0.055992852895975886
1104	54-55	0.037243383859667745
1104	56-57	-0.007496137045258422
1104	58-59	0.01969111443825966
1104	60-61	0.011105457385788498
1104	62-63	-0.03597056170563917
1104	64-65	-0.0847181363760825
1104	66-67	-0.06816252970322267
1104	68-69	-0.09818193090718808
1104	70-71	-0.14204479193765707
1104	72-73	-0.16815418297912288
1104	74-75	-0.16184397986911847
1104	76-77	-0.13036590207993726
1104	78-79	-0.11386029852808832
1104	80-81	-0.19712431738701497
1104	82-83	-0.16237947867232805
1104	84-85	-0.15849371789892786
1104	86-87	-0.13713734491577156
1104	88-89	-0.1595454692840761
1104	90-91	-0.15934692807844897
1104	92-93	-0.13533524232271077
1104	94-95	-0.17664191198764456
1104	96-97	-0.1831303440210732
1104	98-99	-0.23615056631066267
1104	100-101	-0.5451303244261894
1105	1	0.1307923959273225
1105	2	0.09434452252638437
1105	3	0.047184811196075316
1105	4	0.053711857750855074
1105	5	0.026448687225730794
1105	6	0.10451706104121428
1105	7	0.07897831017975676
1105	8	0.08100719526218114
1105	9	0.10317648273012026
1105	10-11	0.11948663084203304
1105	12-13	0.1309179913355507
1105	14-15	0.10466954120347793
1105	16-17	0.14090396946235728
1105	18-19	0.09626029729933094
1105	20-21	0.09864350980171821
1105	22-23	0.09953523725814506
1105	24-25	0.0963421855572264
1105	26-27	0.07635814773359328
1105	28-29	0.051421401692849145
1105	30-31	0.03982674440411671
1105	32-33	0.029576832766011307
1105	34-35	0.008793916970965654
1105	36-37	-0.037207529305106846
1105	38-39	-0.06372528728441296
1105	40-41	-0.13305745836541405
1105	42-43	-0.1260932774658201
1105	44-45	-0.027995191660018293
1105	46-47	-0.026642253582629394
1105	48-49	-0.030610357890900275
1105	50-51	-0.02769091462209161
1105	52-53	-0.01789930179740651
1105	54-55	-0.045041502921868926
1105	56-57	-0.08199182013719053
1105	58-59	-0.0945782689443675
1105	60-61	-0.10717423693423456
1105	62-63	-0.13780015728723072
1105	64-65	-0.1648784542006183
1105	66-67	-0.1772926010430993
1105	68-69	-0.17319404988971598
1105	70-71	-0.1998755187833936
1105	72-73	-0.19756953284932166
1105	74-75	-0.2157498099708377
1105	76-77	-0.20307537943453724
1105	78-79	-0.21214424361234308
1105	80-81	-0.23923627298744776
1105	82-83	-0.2393365912143608
1105	84-85	-0.21249204793758025
1105	86-87	-0.2082382067631272
1105	88-89	-0.21909656829456736
1105	90-91	-0.24017471646727273
1105	92-93	-0.1862264000853031
1105	94-95	-0.1977541921702084
1105	96-97	-0.21590700765785442
1105	98-99	-0.26018210208868453
1105	100-101	-0.6101821152193736
1106	1	0.12562567752749487
1106	2	0.07751341570868675
1106	3	0.09530230118272698
1106	4	0.0995483597402611
1106	5	0.07172898810048878
1106	6	0.10814140998270716
1106	7	0.09012198775747748
1106	8	0.07727688785445963
1106	9	0.07182640476559499
1106	10-11	0.11092876149876929
1106	12-13	0.10562578022711477
1106	14-15	0.12568166483644205
1106	16-17	0.1853257079684809
1106	18-19	0.14299965651781577
1106	20-21	0.1393445289449744
1106	22-23	0.16217783341384262
1106	24-25	0.13811437792234216
1106	26-27	0.11578810716949306
1106	28-29	0.07820045201793135
1106	30-31	0.07743792924603099
1106	32-33	0.06164038313984577
1106	34-35	0.014258901911979649
1106	36-37	-0.006286494171000356
1106	38-39	-0.06690501126810489
1106	40-41	-0.06634836055856397
1106	42-43	-0.03442956970378219
1106	44-45	0.03270409972833477
1106	46-47	0.06483857994469844
1106	48-49	0.05486821529526509
1106	50-51	0.04747346915306139
1106	52-53	0.03440623540834764
1106	54-55	0.026963524314318477
1106	56-57	-0.05486191037839916
1106	58-59	-0.06588917727917476
1106	60-61	-0.07079340261100953
1106	62-63	-0.10996636482209965
1106	64-65	-0.0966438143065318
1106	66-67	-0.10962610897585279
1106	68-69	-0.12206221622111713
1106	70-71	-0.14845886339190173
1106	72-73	-0.12501465093025388
1106	74-75	-0.16445254211178906
1106	76-77	-0.17706999106109578
1106	78-79	-0.1834654098701094
1106	80-81	-0.18927044671934112
1106	82-83	-0.17547979622955978
1106	84-85	-0.15056288854173516
1106	86-87	-0.1796914874082205
1106	88-89	-0.18800221215023072
1106	90-91	-0.19339056080882244
1106	92-93	-0.19220751698433958
1106	94-95	-0.23502418023841187
1106	96-97	-0.23016223254123958
1106	98-99	-0.27842299141487814
1106	100-101	-0.6553759379815673
1107	1	0.10814154485514393
1107	2	0.08439407572984692
1107	3	0.07298688621602878
1107	4	0.04978109486047799
1107	5	0.07194951801871241
1107	6	0.10209055678257783
1107	7	0.05776842701411056
1107	8	0.03277603202117518
1107	9	0.0869072992622506
1107	10-11	0.09596215515745143
1107	12-13	0.12737930385763008
1107	14-15	0.12479214858510801
1107	16-17	0.14811405869083671
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2215	100-101	0.04171817434907865
2216	1	-0.32166910808027893
2216	2	-0.3210277120432252
2216	3	-0.27486075197104043
2216	4	-0.15484086008188314
2216	5	-0.28367861855101495
2216	6	-0.20333605611562433
2216	7	-0.1571642164004956
2216	8	-0.17684810916694715
2216	9	-0.29573124571903975
2216	10-11	-0.2707315381433588
2216	12-13	-0.3033348299192511
2216	14-15	-0.4759751067044462
2216	16-17	-0.5373380936773344
2216	18-19	-0.4101199792502328
2216	20-21	-0.4739675775141947
2216	22-23	-0.480029606551021
2216	24-25	-0.5175054549309479
2216	26-27	-0.4236210666504334
2216	28-29	-0.4144940664356582
2216	30-31	-0.4005118453471681
2216	32-33	-0.36922323291745585
2216	34-35	-0.37635806907227476
2216	36-37	-0.4046668231883217
2216	38-39	-0.3698125313735545
2216	40-41	-0.37867031247500904
2216	42-43	-0.3522073140082824
2216	44-45	-0.4366738506727543
2216	46-47	-0.4858878535742761
2216	48-49	-0.4020697647287719
2216	50-51	-0.35411441101929597
2216	52-53	-0.32426617395885415
2216	54-55	-0.33684427559963837
2216	56-57	-0.32324072323645936
2216	58-59	-0.30781654190028007
2216	60-61	-0.2784371875449736
2216	62-63	-0.35682410171614976
2216	64-65	-0.30130563631487206
2216	66-67	-0.3593036281600561
2216	68-69	-0.36493397603173605
2216	70-71	-0.3483349119100083
2216	72-73	-0.3181286388229907
2216	74-75	-0.27975517237900505
2216	76-77	-0.19672214018220302
2216	78-79	-0.1786101894149965
2216	80-81	-0.12213656993414901
2216	82-83	-0.12710470509354366
2216	84-85	-0.083748064568681
2216	86-87	-0.09162631113584041
2216	88-89	-0.10974898591602056
2216	90-91	-0.15111995108649978
2216	92-93	-0.16051296083141153
2216	94-95	-0.15270223302714214
2216	96-97	-0.1477728358048509
2216	98-99	-0.1268107613625844
2216	100-101	0.009208873088184077
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33391.0
3	11032.0
4	8676.0
5	9175.0
6	23100.0
7	21212.0
8	15189.0
9	16018.0
10	20257.0
11	20424.0
12	18583.0
13	14325.0
14	16186.0
15	15165.0
16	15310.0
17	16226.0
18	17694.0
19	16168.0
20	19352.0
21	24640.0
22	27070.0
23	32182.0
24	37004.0
25	43141.0
26	54676.0
27	72809.0
28	73316.0
29	82089.0
30	100272.0
31	129107.0
32	176311.0
33	253645.0
34	381875.0
35	579228.0
36	1090420.0
37	3143491.0
38	7090722.0
39	3079440.0
40	4848.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.868332661096318	18.85608426600875	15.225676260396682	40.04990681249826
2	14.822468128639944	25.233960342909185	50.24617465995318	9.697396868497698
3	15.509178517170694	23.57820992039136	41.354148222694796	19.558463339743145
4	17.886098623345237	33.003656314233204	33.71619075003011	15.394054312391447
5	22.010033988984034	35.29547558336638	28.569503832084305	14.12498659556528
6	15.867318753588144	37.1337652660945	31.487092091896002	15.511823888421352
7	13.945127152351228	16.873056326988408	50.434554827046775	18.747261693613595
8	15.27526187569602	21.831061531786077	41.96996472214898	20.923711870368926
9	17.974705832561384	22.637800079835966	37.84659922714118	21.54089486046147
10-11	20.244977017036504	31.5400350325991	30.036364668774446	18.178623281589946
12-13	20.054451290805762	23.384677169074063	36.476452311074596	20.084419229045583
14-15	20.109262005506054	27.71939450894827	34.277046128346065	17.89429735719961
16-17	21.433843103810744	28.047280126727387	32.30630559018597	18.2125711792759
18-19	21.199401195888687	28.48083399348344	29.863247427444588	20.456517383183286
20-21	22.454454251799426	27.532393199591365	28.81362282711327	21.19952972149594
22-23	22.572195859174364	29.992372977409186	28.58515437649858	18.85027678691787
24-25	22.68223934096737	29.47486674235481	27.701594753994275	20.141299162683545
26-27	22.75114478522772	28.980243736510353	29.019226112705265	19.24938536555666
28-29	23.229152844169203	29.127329607420965	28.226453501230232	19.4170640471796
30-31	22.457005067753645	30.234582950787704	27.68697424203849	19.62143773942016
32-33	22.050876895361306	28.959071108259355	28.628685141825528	20.36136685455381
34-35	22.832560062840773	30.08985565716735	26.75120737341571	20.32637690657617
36-37	22.65344808095227	29.397744795524446	27.924815396409375	20.023991727113913
38-39	23.29896038104169	27.857245204880808	28.746019768816673	20.097774645260824
40-41	22.286520505771147	29.25919653913067	27.04234675275064	21.411936202347544
42-43	22.788286880195635	29.07932561164967	27.587326748537876	20.545060759616813
44-45	23.546118367208003	27.73315880786395	27.821610838606325	20.899111986321724
46-47	21.216324159898576	28.76250018262588	27.94230274392035	22.078872913555195
48-49	22.07544968013776	28.31690200510984	27.872264099080457	21.735384215671946
50-51	21.523431872220364	28.070745827848654	29.47719466285992	20.928627637071063
52-53	23.11764932746873	27.149502243013718	27.21766108248979	22.51518734702776
54-55	22.134226084526567	27.466065464395424	28.368394098605286	22.031314352472727
56-57	22.85341560755262	27.2377808438718	27.839498065427176	22.069305483148405
58-59	21.89162528279448	28.329560252327447	27.2932659169587	22.48554854791937
60-61	22.611331265859754	28.00510982976723	26.438954234692112	22.944604669680903
62-63	22.886010600838308	26.378933694154817	27.973016514917585	22.762039190089286
64-65	23.016764996998567	26.832816693414642	27.912288564725905	22.23812974486088
66-67	23.77763739281661	26.31152883704778	26.866877785940453	23.043955984195158
68-69	24.003790549559113	26.665539473886458	27.24996819677604	22.080701779778387
70-71	22.515731904216512	26.878480059014176	27.975556910381272	22.630231126388043
72-73	23.63340950570383	26.348517858337583	27.793906840708143	22.22416579525044
74-75	24.705805588506504	27.256134301812796	27.30743876717882	20.73062134250188
76-77	22.693090201455092	26.56897888823205	28.24869452164485	22.489236388668008
78-79	22.382652968773	27.868822016786766	27.402472443748977	22.346052570691256
80-81	23.012690289514516	27.43909408993118	28.296843923214027	21.251371697340275
82-83	23.119754331267952	27.769654168742797	26.872238004163147	22.238353495826104
84-85	23.15101475060972	27.18863419088386	27.67337048218167	21.98698057632475
86-87	23.174694059947885	27.67381706401524	27.94482195403746	21.20666692199941
88-89	23.79795195765151	26.27542060339466	27.734497798342765	22.192129640611068
90-91	24.31271739438796	26.154317011808363	27.339974539723933	22.192991054079748
92-93	24.206334724966336	26.760892712763468	26.588675387823123	22.444097174447066
94-95	24.389639976778007	27.095740725639818	26.326922326927377	22.1876969706548
96-97	24.647486995691427	27.04577330294862	26.543085290052655	21.763654411307296
98-99	25.869875566391826	26.51911007276763	25.964926032403596	21.646088328436942
100-101	26.45495186062638	26.85088078863177	25.19598498469503	21.498182366046816
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	32.0
1	39.5
2	65.0
3	115.0
4	206.5
5	393.5
6	655.0
7	987.0
8	1489.0
9	1944.0
10	2329.0
11	2797.0
12	3263.5
13	3646.5
14	3999.5
15	4400.5
16	4920.0
17	5476.0
18	6103.0
19	7037.0
20	8394.5
21	10114.5
22	13067.0
23	17668.5
24	23666.0
25	33291.0
26	45064.0
27	67197.5
28	95955.0
29	136936.5
30	190601.5
31	227401.5
32	310223.5
33	412799.5
34	574501.5
35	694431.0
36	671569.0
37	690011.0
38	751286.0
39	864745.0
40	931627.5
41	935001.0
42	837185.0
43	709276.5
44	635538.0
45	535493.5
46	485789.0
47	487233.0
48	510096.0
49	503733.0
50	445397.0
51	392646.5
52	398907.0
53	394158.5
54	381440.0
55	359865.5
56	332584.0
57	341343.5
58	335538.0
59	270280.5
60	188005.5
61	134327.0
62	93426.5
63	63158.5
64	42738.0
65	29707.5
66	20485.0
67	12590.5
68	6581.0
69	3953.0
70	2549.5
71	2596.0
72	6199.5
73	15341.5
74	19321.5
75	13802.0
76	7411.0
77	12661.0
78	12281.0
79	2506.0
80	804.5
81	269.5
82	181.5
83	143.5
84	119.5
85	110.0
86	92.0
87	71.5
88	57.5
89	50.5
90	46.5
91	43.5
92	27.5
93	19.0
94	21.5
95	22.5
96	18.0
97	13.5
98	11.5
99	13.0
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2459448234500248
2	0.4124610377588504
3	0.41377621889470156
4	0.44736392174874573
5	0.44175208549939005
6	0.05279172785581616
7	0.016555809592478925
8	0.017014040123974568
9	0.025726371268255353
10-11	0.01693667652774803
12-13	0.15376312302317416
14-15	0.3171818179600065
16-17	0.20369537334154025
18-19	0.3276229279276572
20-21	0.17527912934294682
22-23	0.16207970961752688
24-25	0.12418047403531911
26-27	0.10699087805836893
28-29	0.10281324386213593
30-31	0.08556116190361818
32-33	0.09329454600334008
34-35	0.07972021038851462
36-37	0.11197785449204878
38-39	0.11458143705736493
40-41	0.1273285772971528
42-43	0.1675397941973613
44-45	0.17341347646471456
46-47	0.20539439693559222
48-49	0.22542264178946997
50-51	0.22464305478134103
52-53	0.2423236120420365
54-55	0.2923123972961066
56-57	0.29489515120090026
58-59	0.33565981536642164
60-61	0.38479165001613624
62-63	0.36659930281117287
64-65	0.4616851136194505
66-67	0.4190488455298332
68-69	0.4984953078086231
70-71	0.5103111093707609
72-73	0.5559645577132131
74-75	0.633143671517979
76-77	0.6761727086345927
78-79	0.6543859297280271
80-81	0.7443062327267175
82-83	0.7683960663824884
84-85	0.7973419534629403
86-87	0.8674661023964325
88-89	0.8991197153448134
90-91	0.8832036431826694
92-93	0.9330019949691049
94-95	0.9679257076195227
96-97	0.9879569279963323
98-99	0.9989990935962044
100-101	1.0152900816477541
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.6803769E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.655231382251777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.08144890236622	18.943452032114457
2	8.611474829150298	4.074128592519748
3	3.3328770317777776	2.365199320658875
4	1.730263888854848	1.637191705728648
5	1.043873452438233	1.2346534025608198
6	0.7747662642444713	1.0996365148719474
7	0.5537362030489993	0.9169130605497542
8	0.43467900442900426	0.8225945941419954
9	0.338527244107266	0.7207146259698065
>10	2.4577243834109774	11.581559467013987
>50	0.31017486948938094	5.141445540337479
>100	0.26179974045131155	12.598786524873
>500	0.035246464845611405	5.858264208595125
>1k	0.027260301291817408	13.10944831321258
>5k	0.0036531799737269926	5.97062732189509
>10k+	0.002494240119992912	13.92538477495677
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA	99731	0.5935037550206742	No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT	96179	0.5723656401132389	No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA	95232	0.5667299996804288	No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT	79175	0.47117405624892844	No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT	53244	0.3168574859604414	No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA	49213	0.29286882008435133	No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC	46298	0.2755215213920163	No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA	43812	0.26072722137515697	No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG	41885	0.24925955599603872	No Hit
GTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCTTCTT	41307	0.24581985148688962	No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT	39392	0.23442359865813436	No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG	38168	0.22713951852111275	No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA	37475	0.22301544373765195	No Hit
CCAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTG	37438	0.22279525504069952	No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG	35346	0.2103456670940906	No Hit
CTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCAT	34802	0.2071082981443032	No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT	34207	0.20356742585547327	No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT	33348	0.19845547745865824	No Hit
CTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAAT	33153	0.19729502351526015	No Hit
CTCAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCT	32777	0.1950574302705542	No Hit
GATTTATTTACCTGAAATCATGTCTGTGCAATACCTGTTACCAGCACAGA	31572	0.18788642000494055	No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT	31059	0.18483353347692413	No Hit
GTTCTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTT	30591	0.1820484440127688	No Hit
GTTTATTTTACACTGGGCGAATTGCTTCTGTACAATAGAAAGCAGTCTGT	30535	0.18171518544440834	No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA	30489	0.18144143733468365	No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA	28560	0.16996186986383827	No Hit
CAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCA	27875	0.16588540344728614	No Hit
CAAGAATAGTCTGTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTG	27147	0.16155304205860008	No Hit
CAAGACTTCCTGTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGT	25454	0.15147792141155952	No Hit
CGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAG	25044	0.14903799260749181	No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT	24950	0.1484785942963153	No Hit
AGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATAATAAAT	24871	0.1480084616730925	No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC	23982	0.1427179819003701	No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA	23025	0.13702283100892426	No Hit
CCCAATTCAGGTTAAGATAAGTAGGTTGGCTACTAGGATTCAGTACAAAA	22769	0.13549936326784784	No Hit
GGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGG	22715	0.13517800679121453	No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA	21598	0.1285306885615959	No Hit
GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC	21208	0.1262097806747998	No Hit
GTTTATTTGCGCACAGCCCTGGGATGGAGAGTGGGCTGTGGGTCACCTGA	21198	0.126150270216164	No Hit
CCAATACAAAAGCACCACCCATGCCTCTCAGTACATTGGACCAAGCACCT	21043	0.12522785810730913	No Hit
ATTTTTTTTAACTCACAATCACAACACAGTCCAAGGAGGCCAAACACTTC	20784	0.12368653722864198	No Hit
GTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAAT	20647	0.12287124394533155	No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT	20292	0.12075862266376074	No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC	17875	0.10637494481148843	No Hit
GTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGC	17698	0.10532160969363481	No Hit
GTTGGCTTTTTATTGCTGGCATAGTGCGGGCATAGGGGTCAGGCAGAGTA	17615	0.10482767288695768	No Hit
ATTTACCTGAAATCATGTCTGTGCAATACCTGTTACCAGCACAGAAACTG	17612	0.10480981974936693	No Hit
AAAATGTTCTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTG	17451	0.1038517013653306	No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG	17321	0.10307806540306523	No Hit
ATTTATTTACCTGAAATCATGTCTGTGCAATACCTGTTACCAGCACAGAA	16993	0.10112612235981105	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.54615044993775E-5	0.0	0.0	0.0	0.0
2	6.54615044993775E-5	0.0	0.0	0.0	0.0
3	6.54615044993775E-5	0.0	0.0	0.0	0.0
4	7.736359622653704E-5	0.0	0.0	0.0	0.0
5	8.926568795369658E-5	0.0	0.0	5.9510458635797715E-6	0.0
6	9.521673381727634E-5	0.0	0.0	5.9510458635797715E-6	0.0
7	9.521673381727634E-5	0.0	0.0	5.9510458635797715E-6	0.0
8	9.521673381727634E-5	0.0	0.0	5.9510458635797715E-6	0.0
9	1.0116777968085612E-4	0.0	0.0	5.9510458635797715E-6	0.0
10-11	1.0711882554443589E-4	0.0	0.0	5.9510458635797715E-6	0.0
12-13	1.6067823831665383E-4	0.0	0.0	5.9510458635797715E-6	0.0
14-15	2.08286605225292E-4	0.0	0.0	5.9510458635797715E-6	0.0
16-17	2.529194492021403E-4	0.0	0.0	5.9510458635797715E-6	0.0
18-19	3.421851371558369E-4	0.0	0.0	1.1902091727159543E-5	0.0
20-21	6.27835338607666E-4	0.0	0.0	1.1902091727159543E-5	0.0
22-23	0.0014222999613955655	0.0	0.0	1.487761465894943E-5	0.0
24-25	0.0029546942712673566	0.0	0.0	1.7853137590739315E-5	0.0
26-27	0.005019707185929537	0.0	0.0	2.380418345431909E-5	0.0
28-29	0.008953348501755767	0.0	0.0	2.975522931789886E-5	0.0
30-31	0.016600442436455774	0.0	0.0	2.975522931789886E-5	0.0
32-33	0.030442575115142326	0.0	0.0	2.975522931789886E-5	0.0
34-35	0.04938772962184852	0.0	0.0	3.570627518147863E-5	0.0
36-37	0.07567945024714395	0.0	0.0	5.058388984042806E-5	0.0
38-39	0.11195107478566267	0.0	0.0	5.653493570400783E-5	0.0
40-41	0.1557805275709277	0.0	0.0	5.951045863579772E-5	0.0
42-43	0.20680777032819245	0.0	0.0	5.951045863579772E-5	0.0
44-45	0.27010309413322686	0.0	0.0	5.951045863579772E-5	0.0
46-47	0.33557947624726336	0.0	0.0	6.54615044993775E-5	0.0
48-49	0.41408567327960766	0.0	0.0	7.141255036295726E-5	0.0
50-51	0.4959006518121024	0.0	0.0	8.033911915832691E-5	0.0
52-53	0.5937090661029678	0.0	0.0	8.33146420901168E-5	0.0
54-55	0.7262537350995482	0.0	0.0	8.33146420901168E-5	0.0
56-57	0.8611669203498334	0.0	0.0	8.33146420901168E-5	0.0
58-59	0.9982790170467114	0.0	0.0	8.33146420901168E-5	0.0
60-61	1.1575914903376736	0.0	0.0	8.33146420901168E-5	0.0
62-63	1.3734150951491895	0.0	0.0	8.33146420901168E-5	0.0
64-65	1.5886793016495289	0.0	0.0	8.926568795369658E-5	0.0
66-67	1.8068803492835448	0.0	0.0	9.521673381727634E-5	0.0
68-69	2.0698213597199535	0.0	0.0	9.819225674906623E-5	0.0
70-71	2.302882168875328	0.0	0.0	1.0116777968085612E-4	0.0
72-73	2.552055434706345	0.0	0.0	1.0116777968085612E-4	0.0
74-75	2.858873506294927	0.0	0.0	1.0116777968085612E-4	0.0
76-77	3.1807864057164794	0.0	0.0	1.0116777968085612E-4	0.0
78-79	3.530452007522836	0.0	0.0	1.0414330261264601E-4	0.0
80-81	3.920444871623741	0.0	0.0	1.071188255444359E-4	0.0
82-83	4.344754441696979	0.0	5.9510458635797715E-6	1.1306987140801566E-4	0.0
84-85	4.90051666385083	0.0	5.9510458635797715E-6	1.1306987140801566E-4	8.926568795369658E-6
86-87	5.470237064077708	0.0	5.9510458635797715E-6	1.1902091727159544E-4	1.1902091727159543E-5
88-89	6.124837231456824	0.0	5.9510458635797715E-6	1.2497196313517522E-4	1.1902091727159543E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	3855	0.0	60.875904	1
CGAACGT	2740	0.0	57.387894	1
TCGAACG	1110	0.0	55.209293	2
GCGTGTT	4340	0.0	53.525978	2
GTAAACG	3205	0.0	51.433296	1
TAAACGC	3610	0.0	45.66335	2
GTCGAAC	1420	0.0	43.1563	1
TTATCGT	2595	0.0	39.174404	3
CGGAGTT	2465	0.0	38.73665	1
TATCGTA	2685	0.0	38.038216	4
ATCAATA	25690	0.0	37.33346	4
ATCGTAG	2840	0.0	35.618603	5
TCATCAA	26985	0.0	35.525826	2
GAACGTT	4545	0.0	34.806133	2
CCAAGAC	11030	0.0	34.541542	1
AGTTTCG	2270	0.0	34.110474	4
GACGGAG	3390	0.0	34.052536	1
ACGGAGT	3420	0.0	34.03184	2
CATCAAT	29185	0.0	32.97659	3
CTTACGG	2800	0.0	32.575123	1
>>END_MODULE