FastQCFastQC Report
Fri 27 May 2016
SRR1233126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233126_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16803769
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC2334701.3893906777699694No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA1116550.6644640258979994No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT1041300.6196824057745617No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA791640.47110859474442907No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC706700.42056041117918247No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA701650.4175551330180747No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT692350.41202066036494556No Hit
CTCAGCGTCAGTTCAGTTGCTCACAGCTTCTCACAGCGCATGTTTGACTG574510.3418935359085215No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC546130.3250044677476821No Hit
CTAACTTTGTGTCCGTCTCCATCCATGATTGTCTGTCTCACTGAGCCAAC537810.3200531975891837No Hit
GTCCGTCTCCATCCATGATTGTCTGTCTCACTGAGCCAACTTAACTCTGA496960.2957431752364603No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG484520.28834007418216706No Hit
CTGGTATCTTTCAGAGTGCCAAGTGATTTCAGAATGCAAAGAATTCTTTG483960.28800681561380664No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT373490.2222656119588409No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG367700.21881995640382823No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC358460.21332119002588049No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG330040.1964083176815868No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC329990.19637856245226887No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT321920.19157606844036001No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC316230.18818992334398313No Hit
AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA309650.18427413516574764No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT302320.17991201854774366No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT291710.17359795888648552No Hit
CTTTTGTTTGATGGTTGCGTTCTTGATCTTGTTTGCCATGTAACAGAAAT280930.16718273144554652No Hit
CTAACACTAGCATTATGTATGTGACATATTTCTTTACCAATTTTTACAGC279310.1662186620156466No Hit
CTTTTGTATTGGTATTGTATGATGAGATCAAAAAATATGTGTAATACCCA258850.1540428221787624No Hit
CCTAACACTAGCATTATGTATGTGACATATTTCTTTACCAATTTTTACAG254680.15156123605364963No Hit
GTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCAT241830.14391414211894962No Hit
CCCCTTTCCTGCTCTTGCCTGTGAACAATGGTTAATTGTTCCCAAGAGAG228160.13577906242343607No Hit
CTCTTTTGTTTGATGGTTGCGTTCTTGATCTTGTTTGCCATGTAACAGAA227700.1355053143137114No Hit
CTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA227420.13533868502953117No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA225120.13396994448090782No Hit
TCCGTCTCCATCCATGATTGTCTGTCTCACTGAGCCAACTTAACTCTGAT223710.13313084701414307No Hit
CTTATATTTCTAACAGTTCATTGCTTTGGAATGGCCGTGAAAGAGATCTA216480.1288282408547749No Hit
ATTTCTAACAGTTCATTGCTTTGGAATGGCCGTGAAAGAGATCTAACTGT216260.12869731784577615No Hit
CTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGA215760.12839976555259716No Hit
CGTCTCCATCCATGATTGTCTGTCTCACTGAGCCAACTTAACTCTGATGA214200.1274714023978787No Hit
GTTGCGTTCTTGATCTTGTTTGCCATGTAACAGAAATTACTGCCTGAAAT211000.1255670677215332No Hit
ATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACT210860.12548375307944307No Hit
CCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTT208160.12387697069627653No Hit
CCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTGCTATCATAGCA194520.11575974413835372No Hit
CACATCATTACCCACGGAGAAGAGAAGGACTGAGCCCTGAACCACAGCCT194140.1155336043955377No Hit
CTGCAGTATAGATTTGTGGTGCTAACTTTGTGTCCGTCTCCATCCATGAT193960.11542648556999324No Hit
CACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGG193220.11498610817608836No Hit
GCTAACTTTGTGTCCGTCTCCATCCATGATTGTCTGTCTCACTGAGCCAA187800.11176064131802811No Hit
GGCTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAA186080.11073706142949241No Hit
GGAAGATTGCAAAAGATGAAGGAGCCAACGCTTTCTTCAAAGGTGCTTGG182550.10863634223964873No Hit
CCCGTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGA181870.10823167112092533No Hit
CGTCAGTTCAGTTGCTCACAGCTTCTCACAGCGCATGTTTGACTGTAGTC174910.10408974319987378No Hit
GTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATC174200.10366721894355962No Hit
CCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCC173920.10350058965937939No Hit
CCGCAAACAAAGATCACCCAGCTACTACCATCATTCAAGTAGCACAACTA173370.1031732821368825No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA44850.067.198731
CCCGTCT67500.053.960691
CTCGTAG65900.051.586091
TACGCAT62550.050.6593449
CCGCAAA36300.049.710991
CGGCAAT96100.049.0491681
TCCACTC488700.048.8229142
CTCGTAC22250.047.9333571
CTACGCA67000.047.5777178
CTCTACG66850.047.4735346
CGTCCGT5650.047.1910251
CTGGTAT129650.046.418851
CTCCACT532100.045.375371
CCACTCA527850.045.1387833
CCGAAAC67150.044.8483122
CGCAAAC39450.044.791832
TCTACGC78400.043.9337737
TCGTAGT78500.043.3257682
CAGCGTC133650.043.253823
CACTCAT551600.043.1866534
Produced by FastQC (version 0.11.5)