FastQCFastQC Report
Fri 27 May 2016
SRR1233125_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233125_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43410620
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC3887240.8954583002961026No Hit
CTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTAT2276660.5244477042714433No Hit
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA1916880.44156936712721445No Hit
CGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCAC1821820.4196714997390039No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT1606270.3700177514165888No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT1227580.2827833373492477No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT1152670.26552719127255037No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC1113020.2563934815950567No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA1069820.24644199967657682No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA1035610.2385614395740029No Hit
CTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTT947920.21836131342975518No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA902420.20788000724246739No Hit
CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA894400.2060325330529718No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT888420.20465498995407114No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA877340.20210261912868327No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA871240.20069743302445348No Hit
GTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTG863460.19890524484561614No Hit
CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT855880.19715912834232732No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT849290.19564106663300362No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT849220.19562494154656165No Hit
CTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATAGAT808430.18622862331844145No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG807490.1860120864433634No Hit
TTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATCAC797420.18369237757949552No Hit
CGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACG781730.18007805463271429No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA760790.17525435020278446No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT699270.1610827028040604No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT697330.16063580755123977No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT680660.1567957333942708No Hit
CACGGTCTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTA673990.15525924301472774No Hit
CGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATA672060.15481465134568453No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT662490.15261012167068796No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA640830.14762055920878348No Hit
TTTTATTTCATTTTTCTTGTAATGATATATTATTAATAAAAAACTAACAA636620.14665075043848716No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG631610.14549665496599679No Hit
CTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAAT623970.14373671696004342No Hit
CAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTGTGTAGAAAT595160.1371000920972794No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG580030.13361476984203405No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG562940.1296779451664132No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT552970.12738127214031958No Hit
CCAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTC547220.1260567114682997No Hit
ACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTA519930.11977023133970444No Hit
GACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACT517030.11910219204425093No Hit
CACGTTTTTATTTTTTTATTTCATTTTTCTTGTAATGATATATTATTAAT505400.1164231241111046No Hit
TGGAAATATTGTTAGTGAAGTGGAATAAATTAGGCGAGTATAAAAGAATA489180.1126867112241198No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC486730.11212233319865046No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA463310.10672733999191902No Hit
ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT450300.10373037749748795No Hit
TTTTGAGTTATTGTTGGTTGGAAATATTGTTAGTGAAGTGGAATAAATTA448340.10327887507711248No Hit
TATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGA446620.10288265866739521No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC446270.1028020332351853No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT571550.083.934581
GCGTGTT718000.064.519822
GAGCGGC65500.058.8847779
GATCGGG53950.058.3592761
CGTGTTT850050.053.648833
ATCGGGA58800.053.6266982
CGCGTTT32100.047.6363681
TCGAACG19050.043.6247942
TCGGGAG69200.043.0974273
CGCCGGA47450.042.78469546-47
CGAACGT35350.042.7194371
GTGTTTT1102000.040.0692064
CGCGGGT8350.039.241941
TCGGGGG120150.039.03400838-39
GTAGATA422400.038.708242
GCCGGAT64100.038.22788246-47
CTCGGGG101400.038.22005536-37
CGCGTGC6300.037.689231
CGGGAGA84750.036.8713464
CCGGATC62350.036.7747348-49
Produced by FastQC (version 0.11.5)