FastQCFastQC Report
Fri 27 May 2016
SRR1233125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233125_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43410620
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA2221470.5117342254038297No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG1836780.4231176610700331No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT1428620.32909458561061783No Hit
CTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAG1377710.31736704059974263No Hit
CTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTG1355400.31222774519230545No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT1313050.3024720678949068TruSeq Adapter, Index 7 (97% over 36bp)
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT1299600.299373747714269No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1249610.28785813241091696No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT1234460.2843682029881167No Hit
TATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTA1156130.26632423125953975No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA1126160.2594203906785943No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC1122470.2585703682647242No Hit
CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT1051250.24216424460189695No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA998690.23005660826774646No Hit
CTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTC937810.21603239023077764No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA917520.21135841874638048No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT905300.20854343937036607No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA864240.19908492438025535No Hit
GTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTG860810.19829479514459825No Hit
CTTTTGTTAGTTTTTTATTAATAATATATCATTACAAGAAAAATGAAATA860200.19815427653417528No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC817890.18840781357188632No Hit
CCAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACC765080.1762425876433002No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC754890.1738952357741032No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT747660.1722297447030243No Hit
CGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTAT734940.16929958613813856No Hit
GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT699420.1611172565607218No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC680040.15665291120007038No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG644200.148396866941776No Hit
TAACAATTAAACCTAAACCTCCATAAATAGGTGAAGGCTTTAATGCTAAC641840.1478532211703035No Hit
ATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTC633460.1459228179648206No Hit
TCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGT610790.14070059354139608No Hit
GTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATC564290.1299889289763657No Hit
CAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACCT548820.12642528487268784No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT539090.12418389785725244No Hit
CCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCT537930.12391668213907103No Hit
ATTAAACCTAAACCTCCATAAATAGGTGAAGGCTTTAATGCTAACCCAAG518010.11932794325443866No Hit
CGCCACTTCCCGTTAAGATAATTAACTTTTGTTAGTTTTTTATTAATAAT502400.11573204897787684No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT496110.11428309478187595No Hit
GTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATA482700.11119398893634784No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA481180.11084384420217909No Hit
CTTTTGTATTGGTATTGTATGATGAGATCAAAAAATATGTGTAATACCCA446130.10276978306230135No Hit
CAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGTTGCCGCCA445910.10271910421919797No Hit
GTGCTTTTGTATTGGTATTGTATGATGAGATCAAAAAATATGTGTAATAC434370.1000607685400485No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA353750.048.246881
CTCCGCT22200.042.8658331
CCGAAAC632250.041.5567781
GCCGTCT211250.038.05591250-51
TGCCGTC215650.037.88516250-51
TATGCCG219450.037.65160848-49
CCGTCTT221400.036.8490152-53
CGTCTTC220600.036.46585552-53
ATGCCGT224250.035.86073348-49
CGGCAAT82050.035.4321021
CCAAACG745500.035.0808037
CGAAACC755800.034.861532
TCCGCTC21350.033.829652
CAAACGA794800.033.0243078
CCGCAAA82500.032.9318731
ACTCGTC643050.032.90702442-43
TAACGAG504300.032.68309894-95
TCGTCTA664800.032.0233544-45
TCTCGTA245850.031.76269142-43
TCGGATC41900.031.3001842
Produced by FastQC (version 0.11.5)