FastQCFastQC Report
Fri 27 May 2016
SRR1233124_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233124_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35209504
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTAT3458770.9823398818682593No Hit
CGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCAC2582510.733469576850614No Hit
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA2114500.6005480792913186No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT1792630.5091324206100717No Hit
CTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAAT1742940.4950197537573946No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT1431670.4066146458637986No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT1293020.3672360735328734No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA1263180.3587610890514107No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT1261530.35829246557974803No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA1155160.3280818724398958No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA1047900.29761850663957096No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT1037530.2946732791237275No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC1020890.28994728241556594No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA976910.27745633678906695No Hit
CGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGG922770.26207980663402697No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT899400.25544239418993236No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA867260.24631417699039443No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA831860.2362600734165412No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG824010.23403056174832793No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT766880.21780482905979023No Hit
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC728790.2069867272200142No Hit
CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA709520.20151377309944496No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG685420.19466903027091775No Hit
ATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTAT677790.19250200173225956No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA669370.1901106019556538No Hit
ACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTA629040.1786563082513176No Hit
GACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACT614950.1746545478175438No Hit
GTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTG591160.16789784939884414No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT586540.1665857036781887No Hit
CAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTGTGTAGAAAT584960.16613696120229357No Hit
TATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGA565040.16047939783531173No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG559220.1588264350443562No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG558030.1584884581163086No Hit
CTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATAGAT548980.15591812937779526No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT501710.14249277695022344No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT494610.14047627595094778No Hit
CACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAG459650.1305471386362046No Hit
CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT451000.1280904155877913No Hit
TGGAAATATTGTTAGTGAAGTGGAATAAATTAGGCGAGTATAAAAGAATA444880.12635224852926072No Hit
CGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATA443460.1259489483294056No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC432110.12272538687281707No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC426400.12110366564663903No Hit
TCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATA414220.11764437238309293No Hit
GAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGT407030.11560231010354477No Hit
GTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCTTCTT404500.11488375411366203No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA390920.1110268409347658No Hit
TGTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAA388690.1103934892124581No Hit
GTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCT379570.10780327947817725No Hit
ACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAA378300.10744258141210965No Hit
CGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAG375990.10678650855178193No Hit
CCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAA373580.10610203426892921No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG373530.10608783355766671No Hit
GTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAAT367890.10448599332725618No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA357420.10151236438888772No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA357140.1014328404058177No Hit
CCCAATTCAGGTTAAGATAAGTAGGTTGGCTACTAGGATTCAGTACAAAA356290.10119142831435513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG67450.078.4331361
ATCGGGA71200.073.7029342
CGCGTGT175350.073.420971
GAGCGGC92950.072.841729
GCGTGTT204850.061.9909062
TCGGGAG85250.057.9918373
CGGGAGA98200.051.8923234
CGAACGT29200.051.88051
TCGAACG14250.045.9903532
CGTGTTT286200.044.1555983
CGCCGGA82000.044.01465646-47
GCCGGAT101800.042.84784346-47
TCGGGGG201350.042.72904238-39
CCGGATC96000.042.0529248-49
CTCGGGG170050.041.0008336-37
AGAGCGG174150.040.600368
TCGGAAA39700.039.5956953
CTTACGG83800.039.102221
GTAGATA322950.038.689052
GCGCCGT36200.038.37299344-45
Produced by FastQC (version 0.11.5)