FastQCFastQC Report
Fri 27 May 2016
SRR1233124_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233124_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35209504
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT2664260.756687739764809TruSeq Adapter, Index 20 (97% over 44bp)
CTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAG2329080.6614918517454832No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC2273000.6455643339934581No Hit
CTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTG1985560.5639272850875718No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT1647800.46799864036710087No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA1591280.4519461563559657No Hit
TATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTA1496870.42513237335010456No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT1431060.40644139718639605No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT1177010.3342875832616103No Hit
GTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATA1147070.32578419735762254No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1141090.324085792290627No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA1114350.31649125190743954No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT1110420.3153750760022067No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA1048940.297913881433831No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA1018050.28914068201585574No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC908170.25793319894537564No Hit
ATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTC884860.25131282735479604No Hit
CCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCG864500.24553029772870416No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT847720.24076453902900763No Hit
CTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGT841070.23887584443109453No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC833350.2366832546121638No Hit
CTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTC791340.22475181700940747No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT775860.22035527680253605No Hit
GTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATC754360.21424897095965906No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG699820.19875883511451908No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC674970.19170108161705432No Hit
TCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGT642230.1824024558823663No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG632430.1796191164749154No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC601310.17078059378513255No Hit
CCAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACC599880.17037445344302493No Hit
CTATATTCATAGCACTTATACTAAATAACTCTATAACCATCAACTCAATC596230.16933780152086209No Hit
CGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGT571430.16229424873465984No Hit
AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA568900.1615756927447771No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT554710.15754553088847828No Hit
CTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCA530170.15057582180084103No Hit
CCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTA509290.14464560477761912No Hit
CTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTA498820.14167197583925067No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT483870.13742596317176178No Hit
GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT475500.13504876410641856No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA468250.13298966097335538No Hit
CTCACTTCTATGAAATAAAACTCCAGCAATACTAACTATAATCTCACTGA462450.13134237846690486No Hit
CTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCAATCACA461600.13110096637544227No Hit
TAACAATTAAACCTAAACCTCCATAAATAGGTGAAGGCTTTAATGCTAAC460130.13068346546432463No Hit
CAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACA454600.12911286679869163No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC435720.12375067822596991No Hit
CAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACCT431790.12263450232073703No Hit
ATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTAT426520.12113774735366906No Hit
TAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCA404310.11482979141086452No Hit
TCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCA403790.11468210401373448No Hit
CACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTAT403660.11464518216445198No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA401440.11401467058439675No Hit
CGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTAT395390.11229638452163371No Hit
CACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGG384320.10915234704811519No Hit
CTCAATCTCACTTCTATGAAATAAAACTCCAGCAATACTAACTATAATCT383430.10889957438764261No Hit
CTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGA381510.10835426707516245No Hit
ATTAAACCTAAACCTCCATAAATAGGTGAAGGCTTTAATGCTAACCCAAG372520.1058009791901641No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA360850.10248653318149556No Hit
ATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACT359370.10206619212812541No Hit
CACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCT353150.10029962364706985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA189750.051.455561
CCGCAAA84050.046.0245481
CTCTACG122650.044.2421576
CTCCGCT31550.044.037251
CTACGCA124550.044.0007258
TACGCAT125250.043.9066669
GCCGTCT364350.042.12837650-51
TGCCGTC364950.042.11117650-51
TATGCCG368850.042.0271548-49
CGTCTTC365250.041.8122252-53
TCCGCTC27450.041.718242
ATGCCGT370850.041.36501348-49
CGGCAAT94700.041.0495831
CCGTCTT378650.040.6712552-53
CCGCTCA27450.038.94843
TCTCGTA377050.038.5738942-43
CTCGTAT395300.037.62213544-45
TCGTACC81900.037.3059732
GTATGCC425450.036.513546-47
TATCTCG402750.036.4721540-41
Produced by FastQC (version 0.11.5)