##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1233124_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	35209504
Sequences flagged as poor quality	0
Sequence length	101
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47784777087459	33.0	31.0	34.0	30.0	34.0
2	32.10276958175838	33.0	31.0	34.0	30.0	34.0
3	32.318173042142256	34.0	31.0	34.0	30.0	34.0
4	35.44218035562216	37.0	35.0	37.0	33.0	37.0
5	35.84741418112564	37.0	35.0	37.0	35.0	37.0
6	35.590039922175556	37.0	35.0	37.0	33.0	37.0
7	35.668911609774455	37.0	35.0	37.0	33.0	37.0
8	35.70691827979173	37.0	35.0	37.0	33.0	37.0
9	37.373699924883915	39.0	37.0	39.0	34.0	39.0
10-11	37.47434069221765	39.0	37.0	39.0	34.5	39.0
12-13	37.27913021722772	39.0	37.0	39.0	34.0	39.0
14-15	38.5229107601175	40.0	38.0	41.0	34.0	41.0
16-17	38.430261826465944	40.0	38.0	41.0	33.5	41.0
18-19	38.36930915016582	40.0	38.0	41.0	33.5	41.0
20-21	38.379742895554564	40.0	38.0	41.0	34.0	41.0
22-23	38.36810126606726	40.0	38.0	41.0	34.0	41.0
24-25	38.324783217622155	40.0	38.0	41.0	34.0	41.0
26-27	38.29236138912948	40.0	38.0	41.0	34.0	41.0
28-29	38.23267638192233	40.0	38.0	41.0	34.0	41.0
30-31	38.05272050978054	40.0	38.0	41.0	33.0	41.0
32-33	38.086445338735814	40.0	38.0	41.0	33.0	41.0
34-35	37.96184187939711	40.0	37.5	41.0	33.0	41.0
36-37	37.861422217137736	40.0	37.0	41.0	33.0	41.0
38-39	37.785144672870146	40.0	37.0	41.0	33.0	41.0
40-41	37.66906639752722	40.0	37.0	41.0	32.5	41.0
42-43	37.556916692152214	40.0	37.0	41.0	32.0	41.0
44-45	37.55654095553292	40.0	37.0	41.0	32.0	41.0
46-47	37.760231442056096	40.0	37.0	41.0	33.0	41.0
48-49	37.7047833164591	40.0	37.0	41.0	32.5	41.0
50-51	37.62291796271825	40.0	37.0	41.0	32.5	41.0
52-53	37.47960461186843	40.0	37.0	41.0	32.0	41.0
54-55	37.35853541134803	40.0	37.0	41.0	31.5	41.0
56-57	37.27697557170927	39.5	36.0	41.0	31.0	41.0
58-59	37.1413563224293	39.0	36.0	41.0	31.0	41.0
60-61	36.94068151315054	39.0	36.0	41.0	31.0	41.0
62-63	36.68128224697514	39.0	35.5	40.0	31.0	41.0
64-65	36.39153935539677	38.5	35.0	40.0	30.0	41.0
66-67	36.032365196056155	38.0	35.0	40.0	30.0	41.0
68-69	35.67138176669572	37.0	34.5	40.0	29.0	41.0
70-71	35.30532710713562	37.0	34.0	39.0	29.0	41.0
72-73	34.9063492345703	36.0	34.0	39.0	29.0	41.0
74-75	34.43049828250918	36.0	34.0	39.0	28.0	40.0
76-77	33.95244302504233	35.5	33.5	38.0	28.0	40.0
78-79	33.59996458626625	35.0	33.0	37.0	27.5	39.0
80-81	33.186307026648265	35.0	33.0	37.0	26.0	39.0
82-83	32.857985091184474	35.0	32.5	36.0	26.0	39.0
84-85	32.52979243899601	35.0	32.0	36.0	26.0	37.5
86-87	32.19667824630532	35.0	32.0	36.0	26.0	37.0
88-89	31.92473941126805	35.0	32.0	35.0	26.0	37.0
90-91	31.68604303542589	35.0	32.0	35.0	25.0	36.0
92-93	31.430299713963592	34.5	32.0	35.0	25.0	36.0
94-95	31.194320431210848	34.0	31.5	35.0	24.5	36.0
96-97	31.067973252335506	34.0	32.0	35.0	24.5	36.0
98-99	30.792005377298132	34.0	32.0	35.0	22.0	35.5
100-101	29.707936868409163	34.0	30.5	35.0	10.5	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.0532248489722846
1101	2	0.6318646067614253
1101	3	0.4849625347722153
1101	4	0.8599841222021354
1101	5	0.42644052673153254
1101	6	0.6411715876680333
1101	7	0.5138850071511669
1101	8	0.46286120918075824
1101	9	0.5864288583517165
1101	10-11	0.4521703163918076
1101	12-13	0.5612841540622782
1101	14-15	0.715960713057676
1101	16-17	0.6978429034540099
1101	18-19	0.7620669512338765
1101	20-21	0.7338709761929749
1101	22-23	0.6411072892656691
1101	24-25	0.5862146753769437
1101	26-27	0.5039817947269469
1101	28-29	0.4054016546115875
1101	30-31	0.4126720955533756
1101	32-33	0.21872887255820217
1101	34-35	0.13377792370805253
1101	36-37	0.11358830124456887
1101	38-39	-0.007637072797876954
1101	40-41	-0.1436301960125732
1101	42-43	-0.27064089561636706
1101	44-45	-0.1471276908149619
1101	46-47	0.24636270006038785
1101	48-49	0.27453853056872646
1101	50-51	0.271692639948796
1101	52-53	0.20801213166698318
1101	54-55	0.17389627031471377
1101	56-57	0.026431519820526717
1101	58-59	6.781651447340664E-4
1101	60-61	-0.19309418744727935
1101	62-63	-0.3057465420019625
1101	64-65	-0.25147429527419973
1101	66-67	-0.3503915242741371
1101	68-69	-0.44129644267316337
1101	70-71	-0.6221468094929534
1101	72-73	-0.6484110247224706
1101	74-75	-0.7641761521673089
1101	76-77	-0.7998971284200351
1101	78-79	-0.8572019113461664
1101	80-81	-0.9806348566097753
1101	82-83	-1.1078967906320365
1101	84-85	-1.2379552957701918
1101	86-87	-1.4885994892464218
1101	88-89	-1.6191134860826573
1101	90-91	-1.7693539019651787
1101	92-93	-1.9238620001507023
1101	94-95	-2.1660416733398122
1101	96-97	-2.388999441728224
1101	98-99	-2.744772318654366
1101	100-101	-3.9817019781348435
1102	1	1.057527921977325
1102	2	0.6241201118271604
1102	3	0.4775365789259709
1102	4	0.8515620800028785
1102	5	0.4206895139574556
1102	6	0.6292022966602815
1102	7	0.5223769907343225
1102	8	0.4543149714543375
1102	9	0.5637134456281245
1102	10-11	0.48779593400130494
1102	12-13	0.5913710729275508
1102	14-15	0.6973332866736541
1102	16-17	0.7208402016377988
1102	18-19	0.763724770093205
1102	20-21	0.710879704119229
1102	22-23	0.5845419049767955
1102	24-25	0.5795090191130043
1102	26-27	0.47407396110627076
1102	28-29	0.4044570991605738
1102	30-31	0.4172157910919907
1102	32-33	0.19561906161779063
1102	34-35	0.15164048521840812
1102	36-37	0.0882226697396149
1102	38-39	-0.06925923940394796
1102	40-41	-0.19361577729936386
1102	42-43	-0.2646996440522642
1102	44-45	-0.1098482734329096
1102	46-47	0.18105673650032372
1102	48-49	0.24237891008697687
1102	50-51	0.21948145685184528
1102	52-53	0.19186537192822328
1102	54-55	0.06583919456085141
1102	56-57	-0.06856644020312075
1102	58-59	-0.1190710917833755
1102	60-61	-0.19709384913835493
1102	62-63	-0.24419420690256288
1102	64-65	-0.3042999944202549
1102	66-67	-0.3851578062937264
1102	68-69	-0.4748792094830989
1102	70-71	-0.5882780327950385
1102	72-73	-0.6755466622439101
1102	74-75	-0.7506392086521316
1102	76-77	-0.7986914709353528
1102	78-79	-0.9505640032564173
1102	80-81	-1.0127235347161232
1102	82-83	-1.163188771076527
1102	84-85	-1.2754519328469627
1102	86-87	-1.490787763044743
1102	88-89	-1.5411146643454359
1102	90-91	-1.674013673407142
1102	92-93	-1.89896683868945
1102	94-95	-2.1973835225646
1102	96-97	-2.4610601691072027
1102	98-99	-2.8917943575097276
1102	100-101	-4.041154172555313
1103	1	1.0372570227787108
1103	2	0.6069988542597713
1103	3	0.461796440538798
1103	4	0.8256066127029769
1103	5	0.3874809192738411
1103	6	0.6379426039861329
1103	7	0.47063757611850576
1103	8	0.40828184946684587
1103	9	0.5519902737631526
1103	10-11	0.4180453633842305
1103	12-13	0.5453149117068534
1103	14-15	0.6442347460784887
1103	16-17	0.6779788866247216
1103	18-19	0.6982571939346585
1103	20-21	0.6616319499338914
1103	22-23	0.5988853756952963
1103	24-25	0.5813982263524267
1103	26-27	0.4324708358807854
1103	28-29	0.2918263506843459
1103	30-31	0.31417796316999613
1103	32-33	0.14425515054284688
1103	34-35	0.07832699151781242
1103	36-37	0.007931815070470805
1103	38-39	-0.14351110550127544
1103	40-41	-0.1942763309160611
1103	42-43	-0.26856820691709515
1103	44-45	-0.1366258383147212
1103	46-47	0.13445317864244544
1103	48-49	0.19698691862505058
1103	50-51	0.15578315365193163
1103	52-53	0.13583083332630252
1103	54-55	0.02780643819247075
1103	56-57	-0.11969455927754069
1103	58-59	-0.18475722116455273
1103	60-61	-0.26190131051961174
1103	62-63	-0.2962992925198549
1103	64-65	-0.3591141783071592
1103	66-67	-0.4320604325435369
1103	68-69	-0.5840706352117166
1103	70-71	-0.6153555776818465
1103	72-73	-0.7489828446673314
1103	74-75	-0.882941725071106
1103	76-77	-0.8937391038025524
1103	78-79	-0.9152054694506404
1103	80-81	-1.0697879257741008
1103	82-83	-1.183161752024617
1103	84-85	-1.316295020189834
1103	86-87	-1.5440233066977456
1103	88-89	-1.6592513378704616
1103	90-91	-1.8757824574156636
1103	92-93	-2.1515151291700114
1103	94-95	-2.415887740296192
1103	96-97	-2.699505921418691
1103	98-99	-3.0921271433625783
1103	100-101	-4.348372363644497
1104	1	0.9661621708069532
1104	2	0.5292266750972132
1104	3	0.39496164135292133
1104	4	0.8020894600942441
1104	5	0.35615372427206893
1104	6	0.588404394354491
1104	7	0.4671581593528842
1104	8	0.39761246175851994
1104	9	0.465592782662398
1104	10-11	0.4024768938765959
1104	12-13	0.46312403833995575
1104	14-15	0.6061766906249133
1104	16-17	0.628927671403126
1104	18-19	0.6707458686692789
1104	20-21	0.6254317673225671
1104	22-23	0.5334076165214583
1104	24-25	0.5042147221348685
1104	26-27	0.39064596833613763
1104	28-29	0.22915734175770552
1104	30-31	0.23984405121177588
1104	32-33	0.033049125983460215
1104	34-35	-0.02763364603738694
1104	36-37	-0.06026884775173613
1104	38-39	-0.2075150590354511
1104	40-41	-0.25527927850085064
1104	42-43	-0.34641307461391335
1104	44-45	-0.17208987680285048
1104	46-47	0.08933185054880255
1104	48-49	0.11065841803830523
1104	50-51	0.061647028422079586
1104	52-53	0.06351785438663882
1104	54-55	0.005033972918738527
1104	56-57	-0.1525778383660139
1104	58-59	-0.1936117522086036
1104	60-61	-0.3634663152119586
1104	62-63	-0.39427412850349697
1104	64-65	-0.4270795989703444
1104	66-67	-0.5133681981914648
1104	68-69	-0.6264942447675992
1104	70-71	-0.6892973305346857
1104	72-73	-0.7147597092302718
1104	74-75	-0.8364370960788534
1104	76-77	-0.847752446076143
1104	78-79	-0.998747166912068
1104	80-81	-1.0981929520826625
1104	82-83	-1.2520419521557002
1104	84-85	-1.3659879199439047
1104	86-87	-1.6236505842989537
1104	88-89	-1.6180484668659325
1104	90-91	-1.7580382235270129
1104	92-93	-1.990917966475422
1104	94-95	-2.248333980909024
1104	96-97	-2.5363320998722685
1104	98-99	-2.952672093254627
1104	100-101	-4.049772767571813
1105	1	0.7636838218022675
1105	2	0.45884392750414094
1105	3	0.3343298963151753
1105	4	0.5999736375790263
1105	5	0.12497240893747374
1105	6	0.3841511220928666
1105	7	0.2798676614068185
1105	8	0.2549672838853425
1105	9	0.3314196433203591
1105	10-11	0.27663891298613663
1105	12-13	0.3804680187295375
1105	14-15	0.5073314454972149
1105	16-17	0.5144362190976679
1105	18-19	0.5585994512443051
1105	20-21	0.5260474588451274
1105	22-23	0.4385207646601046
1105	24-25	0.24967274419577024
1105	26-27	0.1741348506614102
1105	28-29	0.19256795282817052
1105	30-31	0.20569171802100072
1105	32-33	-0.044971831481745994
1105	34-35	-0.10851365453896022
1105	36-37	-0.15829504646425363
1105	38-39	-0.29839018499711045
1105	40-41	-0.3765681156226961
1105	42-43	-0.4514375792972487
1105	44-45	-0.28524970647402625
1105	46-47	-0.0075752388841721086
1105	48-49	0.04864223457543915
1105	50-51	0.0458614867270839
1105	52-53	0.02521554572906126
1105	54-55	-0.07785403965674931
1105	56-57	-0.1730775223340757
1105	58-59	-0.24380552507327735
1105	60-61	-0.28085146099488867
1105	62-63	-0.4025782296534359
1105	64-65	-0.4187432182168749
1105	66-67	-0.5929514169223111
1105	68-69	-0.7115606588782626
1105	70-71	-0.8353497902194178
1105	72-73	-0.8845604176160933
1105	74-75	-0.9549165085564866
1105	76-77	-1.0063669432972233
1105	78-79	-1.083977656110477
1105	80-81	-1.192730445976121
1105	82-83	-1.3371767885729255
1105	84-85	-1.436101999711326
1105	86-87	-1.5530273674269637
1105	88-89	-1.5932393460412868
1105	90-91	-1.7751453993831525
1105	92-93	-2.011585591371322
1105	94-95	-2.243743581630092
1105	96-97	-2.5433058991897504
1105	98-99	-2.8998883215062996
1105	100-101	-3.818942810027721
1106	1	0.8786258285223454
1106	2	0.48140088586841756
1106	3	0.3529361671004878
1106	4	0.7347651323617939
1106	5	0.32150046294889023
1106	6	0.5276653837000396
1106	7	0.4128528603508528
1106	8	0.36309686415366826
1106	9	0.44641241124858055
1106	10-11	0.3624777231496239
1106	12-13	0.4529645321604363
1106	14-15	0.5326903156910063
1106	16-17	0.5302257225507887
1106	18-19	0.5742922869267133
1106	20-21	0.5556054739307328
1106	22-23	0.46528393300540927
1106	24-25	0.4689487610212453
1106	26-27	0.3345974813779904
1106	28-29	0.19819703069812533
1106	30-31	0.22826064351748698
1106	32-33	0.03200197074232847
1106	34-35	-0.016047151550260708
1106	36-37	-0.09814316850437166
1106	38-39	-0.1750624157770062
1106	40-41	-0.2278225195546284
1106	42-43	-0.28271260323568015
1106	44-45	-0.1927603880874713
1106	46-47	0.06627323013957209
1106	48-49	0.12186327369469296
1106	50-51	0.11408432065763208
1106	52-53	0.05201603452120196
1106	54-55	-0.009401387092488278
1106	56-57	-0.1701465602371357
1106	58-59	-0.17635949044802857
1106	60-61	-0.24267104241861404
1106	62-63	-0.28277693673091164
1106	64-65	-0.32966602624428987
1106	66-67	-0.3787700533045424
1106	68-69	-0.4098652215999081
1106	70-71	-0.4918591566605457
1106	72-73	-0.5569540593839619
1106	74-75	-0.711222155972564
1106	76-77	-0.672626957274808
1106	78-79	-0.7544011692421222
1106	80-81	-0.7902481258053058
1106	82-83	-0.8832825539149525
1106	84-85	-0.9934775719426128
1106	86-87	-1.1942524151735512
1106	88-89	-1.2092694109704212
1106	90-91	-1.36178314632296
1106	92-93	-1.5015475186554958
1106	94-95	-1.6649758858940444
1106	96-97	-1.8859123280009626
1106	98-99	-2.227652936874943
1106	100-101	-3.2790609114320297
1107	1	0.7736516700264389
1107	2	0.4012155396779704
1107	3	0.29843138596013574
1107	4	0.6825927527734379
1107	5	0.29838810771835256
1107	6	0.5168233838496548
1107	7	0.38892820042322285
1107	8	0.3345007568669658
1107	9	0.4274820907342516
1107	10-11	0.3321911805498132
1107	12-13	0.4345414447621181
1107	14-15	0.5225266633588674
1107	16-17	0.5753757336555978
1107	18-19	0.5932705632189226
1107	20-21	0.5637424232322417
1107	22-23	0.4693591920122486
1107	24-25	0.4983262261453021
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2208	100-101	2.2863415659045465
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2215	4	0.32830342694091286
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2215	14-15	-0.6329184610322187
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2215	22-23	-1.3208081640638838
2215	24-25	-1.4158591900510302
2215	26-27	-1.3902967246076159
2215	28-29	-1.3997534925786184
2215	30-31	-1.3820552993914674
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2216	3	-0.6787726758587951
2216	4	-0.017634360371268087
2216	5	-0.4597216122829124
2216	6	-2.713863733894655
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2216	10-11	-1.7871861661686879
2216	12-13	-2.6745050901294434
2216	14-15	-2.861784107396126
2216	16-17	-1.362703699320214
2216	18-19	-5.933586625048733
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2216	24-25	-4.069912932130961
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2216	28-29	-2.700411123311248
2216	30-31	-6.553793220665515
2216	32-33	-3.1703032451271653
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2216	36-37	-5.0890623877099515
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2216	54-55	-5.119427243575984
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2216	64-65	-4.97720873904629
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2216	68-69	-3.5010352876656015
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2216	76-77	-5.93160663069132
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2216	80-81	-5.196852896796578
2216	82-83	-3.0890478352567
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2216	86-87	-0.8325289525547106
2216	88-89	-0.4597218571003765
2216	90-91	-1.1677016882696094
2216	92-93	-1.6038329583502708
2216	94-95	-1.384870726786879
2216	96-97	-0.9003370856000998
2216	98-99	-0.9181298936835951
2216	100-101	0.09020697408172751
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3896.0
3	1289.0
4	1433.0
5	2358.0
6	3607.0
7	6140.0
8	9693.0
9	13290.0
10	17905.0
11	23748.0
12	30103.0
13	37040.0
14	43891.0
15	48829.0
16	53447.0
17	58766.0
18	64764.0
19	72672.0
20	81304.0
21	92886.0
22	109790.0
23	135657.0
24	171216.0
25	220556.0
26	286874.0
27	372317.0
28	483465.0
29	578464.0
30	681816.0
31	878498.0
32	1145952.0
33	1523103.0
34	2090848.0
35	2956551.0
36	4320517.0
37	6758246.0
38	9041305.0
39	2776412.0
40	10856.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.11052106376774	13.290711970289463	11.001897905251035	50.59686906069176
2	16.5692078994769	20.48765074801005	43.45651249012338	19.48662886238967
3	18.310184494209054	25.367448904875122	27.041052161934697	29.281314438981127
4	19.399508222347844	32.29260202273211	23.77961026780115	24.528279487118898
5	19.163722828788206	36.65804390564495	26.339788475378832	17.838444790188003
6	16.78559893343451	36.044246008662945	26.488546373155042	20.681608684747502
7	12.35691079317675	23.115909272678195	44.371545250964054	20.155634683180995
8	16.890817320232628	23.05472692827482	32.373117212897974	27.68133853859458
9	16.277806695194794	25.096924942986202	34.90297962811757	23.722288733701433
10-11	20.188696779142358	35.75718362860209	24.655641272311023	19.398478319944523
12-13	20.316152422936717	27.814921789298708	28.581760197473955	23.28716559029062
14-15	19.01626441139524	31.244584102143914	28.598799290093403	21.140352196367445
16-17	19.441820310788817	28.162583602427343	30.521835808877057	21.873760277906783
18-19	18.662336169234305	29.051325176293307	29.49852971515873	22.78780893931366
20-21	19.09823121684137	28.43130973147556	29.2541480533564	23.216310998326666
22-23	17.892322482020763	31.472983828457224	29.03729771370821	21.5973959758138
24-25	17.711139209347365	30.812775841592217	29.403403888673896	22.072681060386522
26-27	18.042660374023992	31.359397274120692	29.100953162400156	21.496989189455164
28-29	19.406420962859702	30.11521657274808	28.53104922864107	21.947313235751157
30-31	18.440298175220082	29.76731253120252	29.67496265283156	22.117426640745837
32-33	18.59932261807441	29.82336827053401	29.943368123979713	21.633940987411865
34-35	18.684973855623646	30.739899001231368	28.47754373912213	22.097583404022853
36-37	18.770045541624448	30.690051026602973	28.745304914335694	21.79459851743689
38-39	17.293285104844873	29.97939682653925	29.37523466113916	23.352083407476716
40-41	17.31816216247383	30.745015343375147	29.664265260178425	22.272557233972602
42-43	16.76626568926914	29.793273939222303	31.067916575997838	22.372543795510715
44-45	16.599307906760714	28.72216057068173	30.7110981734321	23.96743334912545
46-47	17.72366698182187	30.541399640185656	30.085746340375465	21.64918703761701
48-49	17.60246545356604	29.759758911866328	30.548191564886512	22.089584069681113
50-51	18.58179904111654	29.672704873220727	31.055562595211107	20.68993349045163
52-53	19.75801334145184	29.865294761870732	28.337436519256094	22.039255377421334
54-55	19.372988605730953	29.511079709349254	29.12130690262074	21.994624782299052
56-57	19.24125406161213	30.36479599688168	28.741781634273263	21.652168307232937
58-59	17.200105709740686	32.126363990010354	29.750657447431905	20.922872852817054
60-61	18.406723801255755	30.720790272021098	29.93656476121866	20.935921165504485
62-63	18.352892955496518	31.155415026400775	29.11760072270838	21.374091295394322
64-65	19.71509277505901	29.342763909014906	28.610148171358237	22.331995144567852
66-67	19.56453949365009	31.439304722377585	27.684681538339195	21.31147424563313
68-69	17.797225842192585	32.22235359927315	28.38389822979721	21.596522328737052
70-71	17.983134489992114	32.51181810124022	28.71072612004918	20.794321288718486
72-73	17.964061385716274	31.72237719827616	29.39672312826612	20.91683828774144
74-75	18.106019641985718	30.08523086189952	30.34099958615286	21.467749909961896
76-77	17.182647760966386	31.967858307726022	28.95307153333404	21.896422397973552
78-79	18.385573075161417	32.78288054357668	28.914362803821646	19.91718357744025
80-81	17.680104675245442	32.71176985172711	27.858675180559345	21.7494502924681
82-83	17.81305925445479	32.06742790567469	28.237503707325917	21.882009132544606
84-85	17.972349719871307	31.495135330576403	28.664046465933335	21.868468483618955
86-87	17.933829622832263	32.16065543767137	28.80695526623917	21.098559673257196
88-89	17.688468865611227	31.334369900111685	29.972414315130646	21.00474691914644
90-91	18.483988344511225	32.32150205572112	28.6646658029321	20.529843796835554
92-93	19.376615674065473	31.06096658700151	29.48145469284702	20.080963046085994
94-95	18.851921271673685	30.992775939436886	29.401307905040046	20.753994883849387
96-97	19.19151490894537	30.544041724495386	29.4769240198675	20.787519346691752
98-99	20.07703770168447	30.95218535093627	26.956228594080333	22.014548353298927
100-101	19.422695568199497	32.11162975927905	27.376697845199764	21.088976827321684
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	114.0
1	100.5
2	86.0
3	96.0
4	126.0
5	157.5
6	173.5
7	187.5
8	239.5
9	297.5
10	342.0
11	435.0
12	619.0
13	769.0
14	1012.0
15	2179.5
16	4476.0
17	9301.5
18	24778.5
19	25475.0
20	18215.0
21	25742.0
22	43762.5
23	76419.0
24	162695.5
25	196883.0
26	190011.0
27	304979.5
28	429020.0
29	542949.5
30	625558.5
31	730522.5
32	996762.0
33	1347517.5
34	1601543.5
35	1698282.5
36	1708278.5
37	1854547.0
38	2008924.5
39	2031656.5
40	2158815.0
41	2099369.0
42	1612504.5
43	1233495.0
44	1165379.0
45	1081936.5
46	968939.0
47	900723.5
48	889187.5
49	944850.5
50	861445.5
51	689147.5
52	596906.0
53	514582.0
54	469830.5
55	434119.0
56	391158.0
57	331870.5
58	273798.0
59	241154.0
60	195479.5
61	135348.5
62	92904.0
63	65470.0
64	50165.0
65	39250.5
66	28211.5
67	18697.5
68	10946.5
69	6298.0
70	5096.0
71	6682.0
72	7763.5
73	10782.5
74	8378.5
75	1730.5
76	775.0
77	496.5
78	222.5
79	130.0
80	68.5
81	43.0
82	40.0
83	31.0
84	23.5
85	18.0
86	15.0
87	14.0
88	10.0
89	7.5
90	4.0
91	2.0
92	2.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13663072334106155
2	2.3857194921007692E-4
3	9.173659475577957E-4
4	2.726536562400879E-4
5	0.0010338117799103333
6	0.054434166411432554
7	0.0
8	0.0
9	1.2212611685753937E-4
10-11	0.0
12-13	0.0
14-15	2.3715187808382644E-4
16-17	0.0
18-19	0.0
20-21	4.4590233364264376E-4
22-23	0.0
24-25	2.769138696188393E-4
26-27	3.734787062038704E-4
28-29	0.0010693135580665948
30-31	0.0028728038884046764
32-33	0.002019341141528151
34-35	0.00440222049137642
36-37	0.0017466874852880632
38-39	0.0051718990418041675
40-41	0.006502505687100847
42-43	0.0035317168909848886
44-45	0.008862663898929108
46-47	0.01013930784142827
48-49	0.013590080678216881
50-51	0.01188031504221133
52-53	0.012326217375853973
54-55	0.01571876729646632
56-57	0.015190500837501146
58-59	0.02115905978113182
60-61	0.016265494680072747
62-63	0.018091706148430833
64-65	0.021852054490742043
66-67	0.01805620437027457
68-69	0.015582440468346273
70-71	0.02631107782716848
72-73	0.02732642868243756
74-75	0.02986977606955213
76-77	0.0221829310631584
78-79	0.02521194277545063
80-81	0.02288586627065238
82-83	0.010998450872809797
84-85	0.020199091699786512
86-87	0.016445843713106555
88-89	0.0054857347607055185
90-91	0.004380919424482662
92-93	0.003111375837614753
94-95	6.248312955502015E-4
96-97	0.001823371326105588
98-99	0.0018162709704743355
100-101	6.248312955502015E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.5209504E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.707867228246295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.63376176346522	11.566293532968304
2	9.91386771474426	3.114514355632007
3	4.285928513891764	2.0196838813790037
4	2.5227232665879074	1.5850640850068252
5	1.6008866824480394	1.2573257727680747
6	1.144268634192759	1.0784411867607955
7	0.8535648273495664	0.9385378085095573
8	0.6504599382601326	0.8173870677986756
9	0.5198218926941841	0.7348763945498343
>10	3.9591722704615124	12.337451412290083
>50	0.46549709901238734	5.113409537432699
>100	0.3572143282435367	11.227102348072192
>500	0.043022532859042485	4.705101360212536
>1k	0.039092312650088785	12.638492359858555
>5k	0.005743916428788851	6.377009380007609
>10k+	0.004974306711206028	24.489309516753384
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT	266426	0.756687739764809	TruSeq Adapter, Index 20 (97% over 44bp)
CTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAG	232908	0.6614918517454832	No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC	227300	0.6455643339934581	No Hit
CTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTG	198556	0.5639272850875718	No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT	164780	0.46799864036710087	No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA	159128	0.4519461563559657	No Hit
TATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTA	149687	0.42513237335010456	No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT	143106	0.40644139718639605	No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT	117701	0.3342875832616103	No Hit
GTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATA	114707	0.32578419735762254	No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT	114109	0.324085792290627	No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA	111435	0.31649125190743954	No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT	111042	0.3153750760022067	No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA	104894	0.297913881433831	No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA	101805	0.28914068201585574	No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC	90817	0.25793319894537564	No Hit
ATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTC	88486	0.25131282735479604	No Hit
CCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCG	86450	0.24553029772870416	No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT	84772	0.24076453902900763	No Hit
CTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGT	84107	0.23887584443109453	No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC	83335	0.2366832546121638	No Hit
CTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTC	79134	0.22475181700940747	No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT	77586	0.22035527680253605	No Hit
GTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATC	75436	0.21424897095965906	No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG	69982	0.19875883511451908	No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC	67497	0.19170108161705432	No Hit
TCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGT	64223	0.1824024558823663	No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG	63243	0.1796191164749154	No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC	60131	0.17078059378513255	No Hit
CCAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACC	59988	0.17037445344302493	No Hit
CTATATTCATAGCACTTATACTAAATAACTCTATAACCATCAACTCAATC	59623	0.16933780152086209	No Hit
CGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGT	57143	0.16229424873465984	No Hit
AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA	56890	0.1615756927447771	No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT	55471	0.15754553088847828	No Hit
CTACAAGCTCTGCACGTCTACCATTCTCAATAAAATTTTTCTTGGTAGCA	53017	0.15057582180084103	No Hit
CCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTA	50929	0.14464560477761912	No Hit
CTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTA	49882	0.14167197583925067	No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT	48387	0.13742596317176178	No Hit
GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT	47550	0.13504876410641856	No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA	46825	0.13298966097335538	No Hit
CTCACTTCTATGAAATAAAACTCCAGCAATACTAACTATAATCTCACTGA	46245	0.13134237846690486	No Hit
CTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCAATCACA	46160	0.13110096637544227	No Hit
TAACAATTAAACCTAAACCTCCATAAATAGGTGAAGGCTTTAATGCTAAC	46013	0.13068346546432463	No Hit
CAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACA	45460	0.12911286679869163	No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC	43572	0.12375067822596991	No Hit
CAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACCT	43179	0.12263450232073703	No Hit
ATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTAT	42652	0.12113774735366906	No Hit
TAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCA	40431	0.11482979141086452	No Hit
TCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCA	40379	0.11468210401373448	No Hit
CACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTAT	40366	0.11464518216445198	No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA	40144	0.11401467058439675	No Hit
CGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTAT	39539	0.11229638452163371	No Hit
CACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGG	38432	0.10915234704811519	No Hit
CTCAATCTCACTTCTATGAAATAAAACTCCAGCAATACTAACTATAATCT	38343	0.10889957438764261	No Hit
CTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGA	38151	0.10835426707516245	No Hit
ATTAAACCTAAACCTCCATAAATAGGTGAAGGCTTTAATGCTAACCCAAG	37252	0.1058009791901641	No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA	36085	0.10248653318149556	No Hit
ATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACT	35937	0.10206619212812541	No Hit
CACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCT	35315	0.10029962364706985	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0015336768163504945	0.0	0.0	2.8401422525009155E-6	0.0
2	0.0015393571008554964	0.0	0.0	5.680284505001831E-6	0.0
3	0.001547877527612999	0.0	0.0	8.520426757502747E-6	0.0
4	0.0015620782388755035	0.0	0.0	8.520426757502747E-6	0.0
5	0.0015649183811280046	0.0	0.0	1.1360569010003662E-5	0.0
6	0.0015762789501380082	0.0	0.0	2.8401422525009156E-5	0.0
7	0.001579119092390509	0.0	0.0	5.112256054501648E-5	0.0
8	0.00158195923464301	0.0	0.0	5.3962702797517396E-5	0.0
9	0.0015904796614005128	0.0	0.0	7.668384081752472E-5	0.0
10-11	0.0016018402304105164	0.0	0.0	7.668384081752472E-5	0.0
12-13	0.0016203011550517724	0.0	0.0	8.236412532252655E-5	0.0
14-15	0.001655802933208034	0.0	0.0	8.946448095377885E-5	0.0
16-17	0.0017055054226267998	0.0	0.0	9.656483658503113E-5	0.0
18-19	0.0017594681254243173	0.0	0.0	1.2638633023629074E-4	0.0
20-21	0.0018006501880855805	0.0	0.0	1.51947610508799E-4	0.0
22-23	0.0018787541000293557	0.0	0.0	1.618881083925522E-4	1.4200711262504577E-6
24-25	0.002012240785896899	0.0	0.0	2.0449024218006592E-4	2.8401422525009155E-6
26-27	0.0022777940865057345	0.0	0.0	2.9111458088134387E-4	2.8401422525009155E-6
28-29	0.0027705587673146435	0.0	0.0	3.2377621678510437E-4	2.8401422525009155E-6
30-31	0.0037986902627199746	0.0	0.0	3.408170703001099E-4	2.8401422525009155E-6
32-33	0.005982759654893179	0.0	0.0	3.5501778156261444E-4	2.8401422525009155E-6
34-35	0.009199220755850467	0.0	0.0	3.7347870620387045E-4	2.8401422525009155E-6
36-37	0.014131127777318307	0.0	0.0	4.288614801276383E-4	2.8401422525009155E-6
38-39	0.021734188587263257	0.0	0.0	4.5726290265264745E-4	2.8401422525009155E-6
40-41	0.03167468647101646	0.0	0.0	4.970248941876603E-4	2.8401422525009155E-6
42-43	0.044212494444681755	0.0	0.0	5.580879526164299E-4	2.8401422525009155E-6
44-45	0.06114258241183971	0.0	0.0	5.935897307726913E-4	2.8401422525009155E-6
46-47	0.08213691394232647	0.0	0.0	6.39032006812706E-4	2.8401422525009155E-6
48-49	0.10888111346300136	0.0	0.0	6.489725046964592E-4	2.8401422525009155E-6
50-51	0.13839019146648587	0.0	0.0	6.518126469489602E-4	2.8401422525009155E-6
52-53	0.17867192903370635	0.0	0.0	6.560728603277116E-4	2.8401422525009155E-6
54-55	0.23354063720977153	0.0	0.0	6.731137138427171E-4	2.8401422525009155E-6
56-57	0.29586329872752537	0.0	0.0	6.816341406002198E-4	2.8401422525009155E-6
58-59	0.3631888708230596	0.0	0.0	6.958348518627243E-4	2.8401422525009155E-6
60-61	0.44100876854158466	0.0	0.0	7.057753497464775E-4	2.8401422525009155E-6
62-63	0.5415086222174559	0.0	0.0	7.185559898827316E-4	2.8401422525009155E-6
64-65	0.6560856409678477	0.0	0.0	7.455373412814904E-4	2.8401422525009155E-6
66-67	0.7843280041661479	0.0	0.0	7.568979102914941E-4	2.8401422525009155E-6
68-69	0.9331883800464784	0.0	0.0	7.668384081752472E-4	2.8401422525009155E-6
70-71	1.0912905788164466	0.0	0.0	8.25061324351516E-4	2.8401422525009155E-6
72-73	1.2765644185160916	0.0	0.0	8.676634581390298E-4	2.8401422525009155E-6
74-75	1.5126313622594627	0.0	0.0	0.001026711424279081	2.8401422525009155E-6
76-77	1.7724433153048675	0.0	0.0	0.0011772389636616294	2.8401422525009155E-6
78-79	2.0688661220561357	0.0	0.0	0.001197119959429136	2.8401422525009155E-6
80-81	2.4065703964475045	0.0	0.0	0.0012397220932166496	2.8401422525009155E-6
82-83	2.7944358432314185	0.0	0.0	0.0012780640136254122	2.8401422525009155E-6
84-85	3.2990368168776247	0.0	0.0	0.0012851643692566644	2.8401422525009155E-6
86-87	3.832381450190267	0.0	0.0	0.0012894245826354156	2.8401422525009155E-6
88-89	4.420996671807703	0.0	0.0	0.0012936847960141671	2.8401422525009155E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGAAA	18975	0.0	51.45556	1
CCGCAAA	8405	0.0	46.024548	1
CTCTACG	12265	0.0	44.242157	6
CTCCGCT	3155	0.0	44.03725	1
CTACGCA	12455	0.0	44.000725	8
TACGCAT	12525	0.0	43.906666	9
GCCGTCT	36435	0.0	42.128376	50-51
TGCCGTC	36495	0.0	42.111176	50-51
TATGCCG	36885	0.0	42.02715	48-49
CGTCTTC	36525	0.0	41.81222	52-53
TCCGCTC	2745	0.0	41.71824	2
ATGCCGT	37085	0.0	41.365013	48-49
CGGCAAT	9470	0.0	41.049583	1
CCGTCTT	37865	0.0	40.67125	52-53
CCGCTCA	2745	0.0	38.9484	3
TCTCGTA	37705	0.0	38.57389	42-43
CTCGTAT	39530	0.0	37.622135	44-45
TCGTACC	8190	0.0	37.305973	2
GTATGCC	42545	0.0	36.5135	46-47
TATCTCG	40275	0.0	36.47215	40-41
>>END_MODULE