FastQCFastQC Report
Fri 27 May 2016
SRR1233123_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233123_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42115946
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA2521550.598716220217397No Hit
CTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTAT2054490.48781760713626143No Hit
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC1911560.4538803426141728No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT1661250.3944467969447961No Hit
CGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCAC1567580.37220581487116544No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT1413370.3355902298858489No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC1373180.32604752603681275No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT1368250.3248769480329375No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA1257950.29868734279410464No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA1215830.2886863802133282No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA1168340.27741036613543No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA1100690.26134756654878416No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT1088930.2585552750020147No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA1071060.25431222653766344No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA973300.2311001158563552No Hit
CTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAAT968300.22991291706946343No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG963760.22883494057096568No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT938180.22276123157722733No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT936840.22244306230234032No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT920550.21857516865464688No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT811580.19270135829312726No Hit
CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA809550.19221935558564918No Hit
GTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTG740340.1757861499774931No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG720190.17100173886631917No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA720010.17095899970999107No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG712000.16905710725339043No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT691760.1642513265640525No Hit
CAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCT691050.16408274433631387No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT628110.14913828600692003No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG609680.1447622712784369No Hit
CAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTGTGTAGAAAT596980.14174678635973179No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC526930.12511413135537783No Hit
CTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATAGAT517260.12281808890152912No Hit
CGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGG516750.12269699462526616No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT511480.12144568710388223No Hit
GTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCT501640.11910927989127917No Hit
CTTAACACAAAAATAAGTACGGATCACGTTTTTATTTTTTTATTTCATTT486520.11551919075971841No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA474640.11269840644206354No Hit
TGGAAATATTGTTAGTGAAGTGGAATAAATTAGGCGAGTATAAAAGAATA469110.11138536458376122No Hit
TGTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAA466640.11079888838303668No Hit
CCAGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTC458910.10896347905850197No Hit
GAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGT457020.10851471791705689No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC442520.10507184143507069No Hit
GTCAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAAT442140.10498161432726692No Hit
GACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACT437860.10396537216568755No Hit
ACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTA436890.10373505560103055No Hit
CGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATA425590.10105198634265511No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA422000.10019957761366681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT311600.079.737571
GATCGGG103000.076.4434051
ATCGGGA107600.072.601792
GAGCGGC118800.072.221889
GCGTGTT385650.062.02582
TCGGGAG117900.061.3462563
CGGGAGA131900.057.7878274
CGAACGT42200.049.176961
CGTGTTT503050.046.418493
CGCCGGA104500.045.1030246-47
GCCGGAT135350.042.98027446-47
CCGGATC124600.042.71585548-49
TCGGGGG266750.042.18698538-39
CTCGGGG212900.041.76042636-37
TCGAACG23550.040.935382
GTAGATA450450.040.7681772
GTCGAAC24350.039.3953631
TCTCGGG189400.038.91763736-37
TCGGAAA50350.038.7654043
TAGATAT491400.037.6807983
Produced by FastQC (version 0.11.5)