FastQCFastQC Report
Fri 27 May 2016
SRR1233123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233123_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42115946
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT3296890.7828127617031326TruSeq Adapter, Index 22 (97% over 40bp)
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC1812760.43042129458519107No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA1633320.38781510452121865No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA1491460.35413190053952487No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT1442670.3425472147770348No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT1371780.3257151103764831No Hit
CTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAG1293490.30712595177133145No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT1237650.293867315719324No Hit
CTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTG1166130.2768856242716239No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA1157870.27492437187567864No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT1109550.2634512827991564No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA1081420.2567721024241032No Hit
TATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTA1021240.24248297782507366No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT999150.23723793358458575No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC970170.23035692941576094No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT901880.21414216838439296No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG854500.20289227267980633No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG853750.20271419286177259No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC773200.1835884204049459No Hit
CCAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACC757840.17994134573161435No Hit
GTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATA736720.17492661805578344No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC724250.1719657442812753No Hit
CTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTC658780.15642056336571425No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT657260.15605965493449916No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG624360.1482478869167512No Hit
TAACAATTAAACCTAAACCTCCATAAATAGGTGAAGGCTTTAATGCTAAC618550.14686836192638295No Hit
ATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTC596780.1416992984082561No Hit
CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT594620.14118642853231886No Hit
CGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTAT580000.13771505927944727No Hit
CTATATTCATAGCACTTATACTAAATAACTCTATAACCATCAACTCAATC563260.13374031774093356No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC561760.13338415810486604No Hit
AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA558730.1326647156400096No Hit
CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT557330.1323322999796799No Hit
CAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACCT529610.12575046990515185No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA523510.12430208738514385No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC523240.12423797865065171No Hit
GTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATC516910.1227349849864467No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT507330.12046031211076202No Hit
CCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCG505690.12007091090866152No Hit
ATTAAACCTAAACCTCCATAAATAGGTGAAGGCTTTAATGCTAACCCAAG496880.1179790666461582No Hit
GTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTG475630.11293347180186811No Hit
GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA475500.11290260463340893No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC467150.11091998265929964No Hit
TCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGT457370.10859782183213931No Hit
GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT456850.10847435315830256No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA448440.10647748479875058No Hit
CTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCAATCACA446630.10604771883789574No Hit
CTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGT444740.10559895769645065No Hit
CTCACTTCTATGAAATAAAACTCCAGCAATACTAACTATAATCTCACTGA440980.10470618420870803No Hit
CTCAATCTCACTTCTATGAAATAAAACTCCAGCAATACTAACTATAATCT425390.10100449839117943No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA424460.10078367941681757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA299850.048.1291771
CCGCAAA100150.042.28431
CTCCGCT28300.042.201451
CGTCTTC483950.042.1370652-53
GCCGTCT486950.042.03211250-51
TGCCGTC482650.041.94896350-51
ATGCCGT489450.041.89038548-49
TATGCCG490200.041.84082848-49
CCGTCTT498150.040.79765752-53
TCTCGTA481000.039.1840242-43
CCGAAAC513600.038.261781
TCCGCTC27150.037.9787752
CTCGTAT511900.037.73737744-45
GTATGCC551750.037.67650646-47
GCTTGAA560700.036.48488260-61
CGGCAAT107000.036.4644931
TGCTTGA565950.035.7813260-61
ATCTCGT523750.035.5957442-43
TACGCAT53200.035.17199
CTACGCA53500.034.175568
Produced by FastQC (version 0.11.5)