##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233123_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42115946 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.481890493448727 33.0 31.0 34.0 30.0 34.0 2 32.09552970744145 33.0 31.0 34.0 30.0 34.0 3 32.31513111447146 34.0 31.0 34.0 30.0 34.0 4 35.43160106150768 37.0 35.0 37.0 33.0 37.0 5 35.849174372101245 37.0 35.0 37.0 35.0 37.0 6 35.58406929764797 37.0 35.0 37.0 33.0 37.0 7 35.66809345799807 37.0 35.0 37.0 33.0 37.0 8 35.705847970267605 37.0 35.0 37.0 33.0 37.0 9 37.37800238418009 39.0 37.0 39.0 34.0 39.0 10-11 37.48032353351388 39.0 37.0 39.0 34.5 39.0 12-13 37.29054730243979 39.0 37.0 39.0 34.0 39.0 14-15 38.545679408459684 40.0 38.0 41.0 34.0 41.0 16-17 38.453431462752846 40.0 38.0 41.0 34.0 41.0 18-19 38.390151701685625 40.0 38.0 41.0 33.5 41.0 20-21 38.394088381631036 40.0 38.0 41.0 34.0 41.0 22-23 38.37784494974896 40.0 38.0 41.0 34.0 41.0 24-25 38.32682661336872 40.0 38.0 41.0 34.0 41.0 26-27 38.30408792194766 40.0 38.0 41.0 34.0 41.0 28-29 38.25206212867687 40.0 38.0 41.0 34.0 41.0 30-31 38.095207798490385 40.0 38.0 41.0 33.0 41.0 32-33 38.129538702514246 40.0 38.0 41.0 33.5 41.0 34-35 38.02255374959404 40.0 38.0 41.0 33.0 41.0 36-37 37.86901196520672 40.0 37.0 41.0 33.0 41.0 38-39 37.80019824320223 40.0 37.0 41.0 33.0 41.0 40-41 37.658429719232714 40.0 37.0 41.0 32.5 41.0 42-43 37.5592054966544 40.0 37.0 41.0 32.0 41.0 44-45 37.54999606324883 40.0 37.0 41.0 32.0 41.0 46-47 37.758261265222444 40.0 37.0 41.0 33.0 41.0 48-49 37.72123452955325 40.0 37.0 41.0 33.0 41.0 50-51 37.65240666801121 40.0 37.0 41.0 32.5 41.0 52-53 37.514833490858784 40.0 37.0 41.0 32.0 41.0 54-55 37.389476411143654 40.0 37.0 41.0 31.5 41.0 56-57 37.32211103604321 40.0 36.5 41.0 31.0 41.0 58-59 37.17836156404987 39.0 36.0 41.0 31.0 41.0 60-61 36.96327578632568 39.0 36.0 41.0 31.0 41.0 62-63 36.693007287548525 39.0 35.5 40.5 31.0 41.0 64-65 36.38932657478476 38.5 35.0 40.0 30.5 41.0 66-67 36.03473439727556 38.0 35.0 40.0 30.0 41.0 68-69 35.670878745071995 37.0 34.5 39.5 29.5 41.0 70-71 35.29484235021101 37.0 34.0 39.0 29.0 41.0 72-73 34.889472529003626 36.0 34.0 39.0 29.0 41.0 74-75 34.4080484503423 36.0 34.0 38.5 28.5 40.0 76-77 33.899129120832285 35.5 33.5 37.5 28.0 40.0 78-79 33.54715102445995 35.0 33.0 37.0 27.5 39.0 80-81 33.13264532868382 35.0 33.0 36.5 26.0 39.0 82-83 32.820083822882665 35.0 33.0 36.0 26.0 38.5 84-85 32.50509516039364 35.0 32.0 36.0 26.0 37.0 86-87 32.19747292391342 35.0 32.0 35.5 26.0 37.0 88-89 31.937040628269397 35.0 32.0 35.0 26.0 36.5 90-91 31.716528995929476 35.0 32.0 35.0 25.5 36.0 92-93 31.483012515022217 35.0 32.0 35.0 25.0 36.0 94-95 31.27269761434303 34.0 32.0 35.0 25.0 36.0 96-97 31.148761207928228 34.5 32.0 35.0 24.5 36.0 98-99 30.883642053297343 35.0 32.0 35.0 23.5 35.5 100-101 29.84302066965325 34.0 30.5 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0478639958718894 1101 2 0.6369143004924851 1101 3 0.4889291460384513 1101 4 0.8674890540115214 1101 5 0.42080173286577605 1101 6 0.6528345588235425 1101 7 0.5427676605926095 1101 8 0.46573093334133375 1101 9 0.5835151853066165 1101 10-11 0.4578261968178907 1101 12-13 0.5691469931207536 1101 14-15 0.7310642387035813 1101 16-17 0.7167201842992057 1101 18-19 0.7973748924786292 1101 20-21 0.7605754301886662 1101 22-23 0.6614921702780663 1101 24-25 0.5989882233487336 1101 26-27 0.5239038001146312 1101 28-29 0.42049877912144495 1101 30-31 0.45578843955535575 1101 32-33 0.25758669725276917 1101 34-35 0.182581901587497 1101 36-37 0.10125032429180436 1101 38-39 0.001794853643097838 1101 40-41 -0.1166289455205245 1101 42-43 -0.23398517228561388 1101 44-45 -0.08417816166215886 1101 46-47 0.28192329936464233 1101 48-49 0.28976769123553936 1101 50-51 0.30793535600063393 1101 52-53 0.26318521904056524 1101 54-55 0.20404555044061112 1101 56-57 0.05048814535467727 1101 58-59 0.02544130150860724 1101 60-61 -0.15034840659483706 1101 62-63 -0.24889677372514996 1101 64-65 -0.22203181299512664 1101 66-67 -0.28339838663555383 1101 68-69 -0.3803758603871117 1101 70-71 -0.49399037604658247 1101 72-73 -0.5528033533564667 1101 74-75 -0.6670755067398701 1101 76-77 -0.7367219692481513 1101 78-79 -0.7800357639938795 1101 80-81 -0.8684285419160602 1101 82-83 -0.9984406700957713 1101 84-85 -1.1293090162605175 1101 86-87 -1.3624829544330126 1101 88-89 -1.4770554742540156 1101 90-91 -1.6138831912726808 1101 92-93 -1.7589706442256272 1101 94-95 -2.0083965692775827 1101 96-97 -2.229556148264084 1101 98-99 -2.5557549416720313 1101 100-101 -3.7670443825472546 1102 1 1.0532797599195654 1102 2 0.6131856216505582 1102 3 0.46867143979496717 1102 4 0.8445434508193088 1102 5 0.41442277655190196 1102 6 0.6146123233819338 1102 7 0.493972716921661 1102 8 0.44247939119039614 1102 9 0.5238132534346889 1102 10-11 0.4514552407837158 1102 12-13 0.5647219005193875 1102 14-15 0.6765205989837639 1102 16-17 0.7015813925825825 1102 18-19 0.7205353376851633 1102 20-21 0.6964930220818744 1102 22-23 0.5680031453522574 1102 24-25 0.5631896700071195 1102 26-27 0.46560760938998413 1102 28-29 0.39550565391380843 1102 30-31 0.416367115350738 1102 32-33 0.18471454148959765 1102 34-35 0.12689670169695688 1102 36-37 0.06826612355587969 1102 38-39 -0.07941888622040949 1102 40-41 -0.20413174774139264 1102 42-43 -0.2568203518670984 1102 44-45 -0.08920565893765087 1102 46-47 0.21675675567744435 1102 48-49 0.2546076672693971 1102 50-51 0.23210662875933963 1102 52-53 0.23866299040593475 1102 54-55 0.12566480887478093 1102 56-57 -0.007486460794339678 1102 58-59 -0.08093635101180752 1102 60-61 -0.15500337001945041 1102 62-63 -0.18904674114482134 1102 64-65 -0.2440675022679244 1102 66-67 -0.3262365513692629 1102 68-69 -0.3978589475467871 1102 70-71 -0.4802124898955782 1102 72-73 -0.5691692783479994 1102 74-75 -0.6665659841705036 1102 76-77 -0.709286314208029 1102 78-79 -0.8277800590478392 1102 80-81 -0.9280952478926068 1102 82-83 -1.0513383191167236 1102 84-85 -1.1791737966727176 1102 86-87 -1.4327376211474672 1102 88-89 -1.4669021229443189 1102 90-91 -1.6285303487581544 1102 92-93 -1.8431717139102801 1102 94-95 -2.110205122015156 1102 96-97 -2.388541288851467 1102 98-99 -2.7976036208203894 1102 100-101 -3.9425892076061047 1103 1 1.0379244688210925 1103 2 0.6199427239371431 1103 3 0.4813817635427995 1103 4 0.8304890121152653 1103 5 0.3944098691006914 1103 6 0.6423915328202412 1103 7 0.47833411411480853 1103 8 0.4359733793414975 1103 9 0.5656219780239695 1103 10-11 0.4460599878355538 1103 12-13 0.5747467031002742 1103 14-15 0.6659689046135355 1103 16-17 0.6992839921623855 1103 18-19 0.7340841989148714 1103 20-21 0.685037556914736 1103 22-23 0.6277910274180627 1103 24-25 0.6007606352001318 1103 26-27 0.4804328549822756 1103 28-29 0.35577166824029405 1103 30-31 0.36620900259002553 1103 32-33 0.2083737814016473 1103 34-35 0.14034696707617655 1103 36-37 0.06386674719094998 1103 38-39 -0.11164744338417876 1103 40-41 -0.16957866050300652 1103 42-43 -0.2341194039327803 1103 44-45 -0.1096816017423734 1103 46-47 0.18950350682396078 1103 48-49 0.26843053566440744 1103 50-51 0.22110294501552374 1103 52-53 0.18740668461337862 1103 54-55 0.08025162344073067 1103 56-57 -0.03844031876623433 1103 58-59 -0.10867734988386246 1103 60-61 -0.20580661620967788 1103 62-63 -0.22029241170022829 1103 64-65 -0.27408765257727197 1103 66-67 -0.3090405350209551 1103 68-69 -0.47895042310552327 1103 70-71 -0.5195689546587232 1103 72-73 -0.6129119930558176 1103 74-75 -0.7601254672741575 1103 76-77 -0.7544453361356958 1103 78-79 -0.8055234764258898 1103 80-81 -0.9230881487475529 1103 82-83 -1.0309990673831635 1103 84-85 -1.1563161076991548 1103 86-87 -1.3439506085809896 1103 88-89 -1.4407172570902844 1103 90-91 -1.667402037402372 1103 92-93 -1.9202366146924525 1103 94-95 -2.1388245383984277 1103 96-97 -2.4132086434654028 1103 98-99 -2.810444886881559 1103 100-101 -4.0890786741637655 1104 1 0.9902871677187157 1104 2 0.5381091818684425 1104 3 0.4244427340839252 1104 4 0.822905737992734 1104 5 0.3826021286587391 1104 6 0.5971939668681614 1104 7 0.47300845699006544 1104 8 0.416332689860468 1104 9 0.47166522402942945 1104 10-11 0.41666971110221596 1104 12-13 0.4906706784999102 1104 14-15 0.6266952178723244 1104 16-17 0.6614309581039635 1104 18-19 0.684009658962097 1104 20-21 0.6516264362500266 1104 22-23 0.5442788953083877 1104 24-25 0.5378856637687761 1104 26-27 0.4038687975307482 1104 28-29 0.27218158406879667 1104 30-31 0.2969490195791522 1104 32-33 0.10372156770085894 1104 34-35 0.03649298357134256 1104 36-37 -0.030044544655190464 1104 38-39 -0.17627843335197468 1104 40-41 -0.229248725570379 1104 42-43 -0.30562813902410113 1104 44-45 -0.11392733153743251 1104 46-47 0.14916344730442432 1104 48-49 0.17623384013420207 1104 50-51 0.10190747240732634 1104 52-53 0.13582914460806705 1104 54-55 0.0616273306297046 1104 56-57 -0.07832479261026037 1104 58-59 -0.1363630638832234 1104 60-61 -0.2987020435918808 1104 62-63 -0.31188818725118495 1104 64-65 -0.35106873898726576 1104 66-67 -0.4173519770597949 1104 68-69 -0.5445248065067787 1104 70-71 -0.604741949854585 1104 72-73 -0.6645348212633735 1104 74-75 -0.7409920583030569 1104 76-77 -0.753152795936856 1104 78-79 -0.8937834638142235 1104 80-81 -1.0094602583617345 1104 82-83 -1.1335042771276491 1104 84-85 -1.2111816242938467 1104 86-87 -1.4289600321995124 1104 88-89 -1.4608549521633911 1104 90-91 -1.5884819249893916 1104 92-93 -1.8094298761111531 1104 94-95 -2.0228265708035558 1104 96-97 -2.2811409468532737 1104 98-99 -2.670022353852527 1104 100-101 -3.734662471391868 1105 1 0.7756159469273065 1105 2 0.4793504737643204 1105 3 0.3459482675535668 1105 4 0.6085363632270102 1105 5 0.13229368201358938 1105 6 0.3677640402474438 1105 7 0.29233102888291285 1105 8 0.23893022088114435 1105 9 0.3325987190687627 1105 10-11 0.27147179385266895 1105 12-13 0.38423364221125667 1105 14-15 0.5267363301416452 1105 16-17 0.5271175969692337 1105 18-19 0.5569889066227347 1105 20-21 0.5467616841904501 1105 22-23 0.4569908557634079 1105 24-25 0.2523886963778068 1105 26-27 0.22024968344752693 1105 28-29 0.20478806924347026 1105 30-31 0.23020232911539296 1105 32-33 -3.1697674311459423E-4 1105 34-35 -0.07195761131247025 1105 36-37 -0.15188039276170429 1105 38-39 -0.25655547853146743 1105 40-41 -0.34768304186905397 1105 42-43 -0.3980210195625489 1105 44-45 -0.2571099011863893 1105 46-47 0.007605548454051814 1105 48-49 0.05079132276305387 1105 50-51 0.07452423108021122 1105 52-53 0.054785162206677285 1105 54-55 -0.05887947458283094 1105 56-57 -0.14326180264916388 1105 58-59 -0.23497179792821044 1105 60-61 -0.2896344672459321 1105 62-63 -0.40330068718623124 1105 64-65 -0.4209095889571728 1105 66-67 -0.5083607427347374 1105 68-69 -0.6251020057069994 1105 70-71 -0.7282730060008049 1105 72-73 -0.7816766406910176 1105 74-75 -0.8169851553722722 1105 76-77 -0.8702682788148479 1105 78-79 -0.9373511862823563 1105 80-81 -1.032541153360782 1105 82-83 -1.1919508639192067 1105 84-85 -1.2959667117562503 1105 86-87 -1.4243075459859753 1105 88-89 -1.4358732015553706 1105 90-91 -1.5766150738300944 1105 92-93 -1.7682993989023714 1105 94-95 -2.034577059558746 1105 96-97 -2.2884536264942206 1105 98-99 -2.6460341039091375 1105 100-101 -3.6282909496453293 1106 1 0.8982059222940144 1106 2 0.499070519999087 1106 3 0.35125598341954856 1106 4 0.7420160230431634 1106 5 0.32055167760684355 1106 6 0.5273396383752598 1106 7 0.4034501958009926 1106 8 0.33278583604165846 1106 9 0.4596300523086967 1106 10-11 0.3627574280981207 1106 12-13 0.45339104333059765 1106 14-15 0.5374295465149856 1106 16-17 0.5602826012118882 1106 18-19 0.6077955640234478 1106 20-21 0.5649659624059922 1106 22-23 0.4869958713171627 1106 24-25 0.48303830552008264 1106 26-27 0.3345972330513902 1106 28-29 0.208035767193401 1106 30-31 0.24798756150975265 1106 32-33 0.07398231384765097 1106 34-35 0.021422556335210174 1106 36-37 -0.10713326025262404 1106 38-39 -0.1812942583317465 1106 40-41 -0.22943389971054273 1106 42-43 -0.2554885774269664 1106 44-45 -0.17247935710429374 1106 46-47 0.10657662989861905 1106 48-49 0.16793625487377994 1106 50-51 0.1429054581852185 1106 52-53 0.07609272022563118 1106 54-55 0.008975404346102778 1106 56-57 -0.17914747090061667 1106 58-59 -0.17643548181092683 1106 60-61 -0.21363792122860303 1106 62-63 -0.25373491796008807 1106 64-65 -0.2737522840003521 1106 66-67 -0.36152209355724807 1106 68-69 -0.38206462129422647 1106 70-71 -0.453673142324746 1106 72-73 -0.5096716153323726 1106 74-75 -0.6674562643143034 1106 76-77 -0.645499449765353 1106 78-79 -0.6736634511794577 1106 80-81 -0.7382510936556415 1106 82-83 -0.8140903765704977 1106 84-85 -0.9517256360958584 1106 86-87 -1.1361264720904671 1106 88-89 -1.1316185427232348 1106 90-91 -1.2666991185758611 1106 92-93 -1.4294140507938806 1106 94-95 -1.5974232753685378 1106 96-97 -1.8307501423187311 1106 98-99 -2.1368178398069197 1106 100-101 -3.153534593569063 1107 1 0.7746203724195304 1107 2 0.39607739612795 1107 3 0.2912400580155463 1107 4 0.6960060885065857 1107 5 0.2946400285496864 1107 6 0.5285957774321162 1107 7 0.3994354374075968 1107 8 0.32961443452600037 1107 9 0.40583604489533087 1107 10-11 0.33716561754036434 1107 12-13 0.43312936927823387 1107 14-15 0.5029696920118027 1107 16-17 0.5478134127294609 1107 18-19 0.5813824598231179 1107 20-21 0.5685245248903357 1107 22-23 0.4784605025763966 1107 24-25 0.49048253834534705 1107 26-27 0.3890182134610498 1107 28-29 0.28912136678130906 1107 30-31 0.3093108746687179 1107 32-33 0.10749777810202943 1107 34-35 0.03217813986996276 1107 36-37 0.019876469269334507 1107 38-39 -0.1221726080131802 1107 40-41 -0.20349491818325305 1107 42-43 -0.27730954262926133 1107 44-45 -0.1462896503180744 1107 46-47 0.1469793299554496 1107 48-49 0.22209067577904307 1107 50-51 0.23550866263705217 1107 52-53 0.1913987666745598 1107 54-55 0.07602780140550891 1107 56-57 -0.004255695240630075 1107 58-59 -0.013632691142852593 1107 60-61 -0.06058737057028196 1107 62-63 -0.11395714577959382 1107 64-65 -0.10246900571058859 1107 66-67 -0.15241128242075774 1107 68-69 -0.24664671910628755 1107 70-71 -0.29089668714926376 1107 72-73 -0.3412619275028135 1107 74-75 -0.39827705259400403 1107 76-77 -0.3980341326172976 1107 78-79 -0.4492070854655168 1107 80-81 -0.5164403288121164 1107 82-83 -0.6683805193218504 1107 84-85 -0.7197391512400912 1107 86-87 -0.8753550276595519 1107 88-89 -0.8485436772353374 1107 90-91 -0.9566104050014665 1107 92-93 -1.0497152745145684 1107 94-95 -1.2147061259717837 1107 96-97 -1.4624068607919263 1107 98-99 -1.7249575200985277 1107 100-101 -2.5945344829947494 1108 1 -0.7030628569771658 1108 2 -0.14172602762220876 1108 3 0.022572249994723848 1108 4 0.6082517745135121 1108 5 0.21850328174905087 1108 6 0.4712620683494322 1108 7 0.3189208854752721 1108 8 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-0.5248945043634592 2215 78-79 -0.40512537218474165 2215 80-81 -0.7110495929672638 2215 82-83 -0.6244258181451414 2215 84-85 -0.7536129154088442 2215 86-87 -0.9647685194354523 2215 88-89 -0.22459576212992616 2215 90-91 -0.4500280481855299 2215 92-93 -0.3068685554906594 2215 94-95 0.3875641760192323 2215 96-97 0.7316168976475801 2215 98-99 0.897883346601045 2215 100-101 1.309469243901983 2216 1 -0.04222640091863994 2216 2 -0.47692345580512097 2216 3 -0.663888505140708 2216 4 0.007258833685952482 2216 5 -0.4560723189932787 2216 6 -2.7123329323572065 2216 7 -1.0085119091934445 2216 8 -2.5183853549839696 2216 9 -2.9596304998676217 2216 10-11 -1.756808864215074 2216 12-13 -2.64423293950464 2216 14-15 -2.8677144872378477 2216 16-17 -1.3663429478311002 2216 18-19 -5.9663359333564046 2216 20-21 -3.5142071359029785 2216 22-23 -1.631536318386452 2216 24-25 -4.043628784874983 2216 26-27 -3.207820740852945 2216 28-29 -2.694004655910625 2216 30-31 -6.57580153774051 2216 32-33 -3.1861300424067167 2216 34-35 -4.4168633477427335 2216 36-37 -5.017648532476201 2216 38-39 -4.224598283488746 2216 40-41 -3.578608217644252 2216 42-43 -3.4854272792156067 2216 44-45 -4.545007173907813 2216 46-47 -7.778878167092962 2216 48-49 -4.96134761159832 2216 50-51 -7.15717820863788 2216 52-53 -6.94798264866229 2216 54-55 -5.036409703788976 2216 56-57 -4.210652319212869 2216 58-59 -3.847818918723611 2216 60-61 -2.4778705036005846 2216 62-63 -3.5191779706133275 2216 64-65 -4.885768156077688 2216 66-67 -4.669871912112271 2216 68-69 -3.372653921029446 2216 70-71 -2.654048470175006 2216 72-73 -3.2031308272025427 2216 74-75 -4.123274506253608 2216 76-77 -5.731419155420674 2216 78-79 -5.570755488308613 2216 80-81 -5.049695942552546 2216 82-83 -2.93822944329942 2216 84-85 -2.59177852905448 2216 86-87 -0.7262360411541593 2216 88-89 -0.3634265315814673 2216 90-91 -1.122558261885004 2216 92-93 -1.5531757406404871 2216 94-95 -1.3118905620309356 2216 96-97 -0.8312051579137041 2216 98-99 -0.8458048879728146 2216 100-101 0.18845268769523216 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4668.0 3 1549.0 4 1894.0 5 2975.0 6 4582.0 7 7729.0 8 12164.0 9 16705.0 10 22116.0 11 29168.0 12 36646.0 13 44952.0 14 52195.0 15 58111.0 16 63118.0 17 69665.0 18 78410.0 19 87287.0 20 97029.0 21 110743.0 22 130236.0 23 160206.0 24 204227.0 25 262903.0 26 341035.0 27 447057.0 28 578743.0 29 679446.0 30 801059.0 31 1032789.0 32 1346817.0 33 1796866.0 34 2470297.0 35 3522182.0 36 5172158.0 37 8161399.0 38 1.0905136E7 39 3289997.0 40 11687.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.58474130368179 13.594044519264198 11.416808090994827 47.40440608605919 2 18.107752987213075 21.561524446714163 40.187669039708666 20.143053526364092 3 19.26929996442405 26.212795464931926 26.456976168720274 28.060928401923746 4 19.760056909731308 33.532971695671606 22.672815108431937 24.03415628616515 5 20.381902195379634 37.24915734695745 24.62925581190486 17.739684645758054 6 17.094593063247267 36.77032386485848 25.9679866376828 20.16709643421145 7 12.814255674086011 23.763479039506795 43.22408429339329 20.198180993013906 8 16.842934027885782 24.012054721506196 31.749088575619318 27.39592267498871 9 16.963161541940032 24.978836380222628 33.8265626727005 24.231439405136847 10-11 19.96591504794882 37.280043525556806 23.46883292138327 19.285208505111104 12-13 20.252357622454927 28.873120883952126 27.895846148154906 22.97867534543804 14-15 18.93631001208156 32.002489324533876 27.960224231534724 21.100976431849844 16-17 19.383586919785678 28.965754443696934 29.52932720542476 22.12133143109263 18-19 18.540301813474642 29.998584146726753 28.898639721876364 22.562474317922245 20-21 19.56809427025835 29.265657756254193 28.373323248115323 22.792924725372135 22-23 18.116470659355485 32.68396606833905 28.17102339337219 21.028539878933266 24-25 17.896313821155392 31.68247168790551 28.89447757595213 21.526736914986962 26-27 18.073625089541856 32.02388705667774 28.37006766513805 21.532420188642362 28-29 19.189824963272432 30.90766644986606 27.736512425447884 22.16599616141362 30-31 18.3114205518312 30.36668915288228 29.31244655962088 22.00944373566563 32-33 18.41633566883881 30.41591159271216 29.73852358822541 21.429229150223623 34-35 18.67320850718668 31.54559738272557 27.561595847845517 22.219598262242236 36-37 18.178276918568407 32.074619475311884 28.468835749606292 21.27826785651342 38-39 17.70076003100413 30.863743586787614 28.93843025231016 22.497066129898098 40-41 17.339589162965883 31.84623276541724 28.846226544081983 21.967951527534893 42-43 16.821699841719322 30.917153402087127 30.250118326434787 22.01102842975876 44-45 16.79268632846075 29.75401360161049 29.97330188270745 23.47999818722131 46-47 17.7357463512277 31.372653294195956 29.23603981504934 21.655560539527 48-49 17.634034297031818 30.61565175546591 30.112859619542277 21.637454327959993 50-51 18.357655618854025 30.296916000370448 30.732597735978096 20.612830644797427 52-53 19.364605684672835 30.608443880617532 28.191724380903466 21.835226053806167 54-55 19.354971582386536 30.582212547531345 28.823064213534323 21.239751656547792 56-57 18.83731722989077 30.957903780323754 28.401377387685617 21.80340160209986 58-59 17.285675383110117 32.94241562501748 29.0234531507022 20.748455841170205 60-61 18.407509987326343 31.50734252309646 29.41596738511376 20.669180104463436 62-63 18.362589696827847 31.827147821331724 28.694955616058003 21.115306865782422 64-65 19.66099403725084 30.446019678744157 28.132634167555835 21.760352116449162 66-67 19.28359931379788 32.370593840030516 27.421407227312685 20.924399618858928 68-69 17.674143180675163 33.123184515643416 28.172899915709802 21.029772387971622 70-71 17.68171319751066 33.815387101855606 28.096110946079694 20.406788754554043 72-73 17.587049638755758 32.6121780465429 29.206982681761133 20.593789632940208 74-75 17.851752414737096 31.117293103665926 29.959290948038632 21.071663533558347 76-77 17.011928849687887 33.063315043717694 28.630250973444493 21.294505133149926 78-79 18.0673152198018 33.70936590170884 28.27097087923392 19.952347999255437 80-81 17.4928342444469 33.78047210994057 27.510683886282028 21.216009759330504 82-83 17.708863365158425 33.17028315117128 27.782357114224666 21.33849636944563 84-85 17.913518771252477 32.84398824959079 28.291694412746654 20.950798566410082 86-87 17.655195216728085 33.48029468437766 28.294606816888688 20.569903282005566 88-89 17.65194135034011 32.60040788999543 29.197198845250803 20.55045191441365 90-91 18.35182424890254 33.45937794145392 28.052927978907565 20.135869830735974 92-93 18.891085342667072 32.433705150131715 28.88686174824991 19.788347758951303 94-95 18.478884385129614 32.3070687139073 28.796902274950746 20.41714462601234 96-97 18.52806395725014 32.2792854155044 28.774414488056387 20.41823613918907 98-99 19.43889797871467 32.43681090353277 26.629334106135065 21.49495701161749 100-101 19.055713714298115 33.458609018909094 26.693098717981044 20.79257854881174 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 318.0 1 345.5 2 370.0 3 432.5 4 537.0 5 636.5 6 710.5 7 721.5 8 800.0 9 912.0 10 978.5 11 1242.5 12 1770.5 13 2107.0 14 2571.0 15 5693.0 16 11661.0 17 23681.5 18 58076.0 19 56126.0 20 31444.5 21 38682.5 22 59411.0 23 105601.5 24 204729.5 25 252006.0 26 279796.5 27 424714.0 28 570861.0 29 707013.5 30 815531.5 31 955973.0 32 1248485.5 33 1591741.5 34 1856953.5 35 1984139.0 36 2040607.0 37 2264983.5 38 2447528.5 39 2424354.5 40 2533185.5 41 2474851.0 42 1942894.5 43 1539172.5 44 1448085.0 45 1319673.0 46 1179976.0 47 1083468.0 48 1045522.5 49 1062240.0 50 957025.0 51 794867.5 52 683533.0 53 572729.5 54 507348.0 55 460796.0 56 412273.5 57 354191.5 58 295952.0 59 253255.0 60 200713.5 61 140513.5 62 97777.5 63 70887.5 64 55133.0 65 42039.5 66 29367.0 67 19632.0 68 11673.5 69 6854.5 70 6240.0 71 7885.5 72 9475.5 73 11215.0 74 7568.0 75 1838.0 76 854.5 77 509.5 78 233.5 79 124.5 80 62.5 81 45.5 82 33.5 83 30.5 84 27.5 85 19.5 86 16.5 87 12.0 88 8.5 89 7.5 90 7.0 91 6.0 92 4.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13519819785123668 2 2.421885525259245E-4 3 9.85374993120183E-4 4 2.991740942967303E-4 5 0.0010708533057763917 6 0.05377535625105038 7 0.0 8 0.0 9 9.735030052512652E-5 10-11 0.0 12-13 0.0 14-15 2.4575014888659986E-4 16-17 0.0 18-19 0.0 20-21 4.3095315964171866E-4 22-23 0.0 24-25 2.635581306899767E-4 26-27 3.965243948218568E-4 28-29 0.0010494837276123397 30-31 0.0029822433526721683 32-33 0.002056228298896575 34-35 0.004525601775631491 36-37 0.0018389709208953779 38-39 0.005231985053832104 40-41 0.006648313206594006 42-43 0.003606709914577248 44-45 0.009023897979164471 46-47 0.01016598321215437 48-49 0.013772693126731617 50-51 0.011981210157311913 52-53 0.012603302321643209 54-55 0.01605686359271142 56-57 0.015489382572577141 58-59 0.02127341506231393 60-61 0.016510373529304078 62-63 0.01814633345764096 64-65 0.021830211293366174 66-67 0.018171264632165687 68-69 0.015813487841398598 70-71 0.02651845930280184 72-73 0.027559632638905936 74-75 0.030051562892591796 76-77 0.022649378456321506 78-79 0.025366876479516808 80-81 0.023219233874029566 82-83 0.011106244651372665 84-85 0.020530228621719666 86-87 0.016689640546124738 88-89 0.005664125412260715 90-91 0.004481675420516495 92-93 0.0031900031403782306 94-95 6.29215357052647E-4 96-97 0.0019339468238467209 98-99 0.0018722124869283477 100-101 7.598072236107436E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4.2115946E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.448884436245237 #Duplication Level Percentage of deduplicated Percentage of total 1 75.9316642839683 14.767861637106694 2 9.932826316082236 3.8636478229355777 3 3.9806710333738797 2.3225883272039245 4 2.2143773110491702 1.7226867368335512 5 1.4082281582784306 1.3694233355111833 6 0.9841085663475592 1.1483888267767592 7 0.7061384333215346 0.9613523349963251 8 0.5547267102569244 0.8631052545188336 9 0.41597816960693196 0.7281280213817436 >10 3.1440400703929727 12.071937724523583 >50 0.3727600984792283 5.064820491432412 >100 0.28304846588367105 11.05747930353195 >500 0.03357200833847814 4.538208896976977 >1k 0.029759095344359 11.891888239924311 >5k 0.004101114898548905 5.556346365702247 >10k+ 0.004000164377946792 22.07213668064405 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT 329689 0.7828127617031326 TruSeq Adapter, Index 22 (97% over 40bp) CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC 181276 0.43042129458519107 No Hit CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA 163332 0.38781510452121865 No Hit CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA 149146 0.35413190053952487 No Hit CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT 144267 0.3425472147770348 No Hit CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT 137178 0.3257151103764831 No Hit CTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAG 129349 0.30712595177133145 No Hit CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT 123765 0.293867315719324 No Hit CTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTG 116613 0.2768856242716239 No Hit CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA 115787 0.27492437187567864 No Hit GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT 110955 0.2634512827991564 No Hit CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA 108142 0.2567721024241032 No Hit TATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTA 102124 0.24248297782507366 No Hit CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT 99915 0.23723793358458575 No Hit GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC 97017 0.23035692941576094 No Hit CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT 90188 0.21414216838439296 No Hit GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG 85450 0.20289227267980633 No Hit CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG 85375 0.20271419286177259 No Hit CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC 77320 0.1835884204049459 No Hit CCAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACC 75784 0.17994134573161435 No Hit GTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATA 73672 0.17492661805578344 No Hit CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC 72425 0.1719657442812753 No Hit CTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTC 65878 0.15642056336571425 No Hit CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT 65726 0.15605965493449916 No Hit CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG 62436 0.1482478869167512 No Hit TAACAATTAAACCTAAACCTCCATAAATAGGTGAAGGCTTTAATGCTAAC 61855 0.14686836192638295 No Hit ATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTC 59678 0.1416992984082561 No Hit CTCCAACCCTCACACACACGAGAACTAACACTAATAGCCCTTCACATAAT 59462 0.14118642853231886 No Hit CGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTAT 58000 0.13771505927944727 No Hit CTATATTCATAGCACTTATACTAAATAACTCTATAACCATCAACTCAATC 56326 0.13374031774093356 No Hit CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC 56176 0.13338415810486604 No Hit AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA 55873 0.1326647156400096 No Hit CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT 55733 0.1323322999796799 No Hit CAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACCT 52961 0.12575046990515185 No Hit GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA 52351 0.12430208738514385 No Hit CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC 52324 0.12423797865065171 No Hit GTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATC 51691 0.1227349849864467 No Hit CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT 50733 0.12046031211076202 No Hit CCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCG 50569 0.12007091090866152 No Hit ATTAAACCTAAACCTCCATAAATAGGTGAAGGCTTTAATGCTAACCCAAG 49688 0.1179790666461582 No Hit GTCCGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTG 47563 0.11293347180186811 No Hit GTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAA 47550 0.11290260463340893 No Hit CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC 46715 0.11091998265929964 No Hit TCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGT 45737 0.10859782183213931 No Hit GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT 45685 0.10847435315830256 No Hit CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA 44844 0.10647748479875058 No Hit CTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCAATCACA 44663 0.10604771883789574 No Hit CTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGT 44474 0.10559895769645065 No Hit CTCACTTCTATGAAATAAAACTCCAGCAATACTAACTATAATCTCACTGA 44098 0.10470618420870803 No Hit CTCAATCTCACTTCTATGAAATAAAACTCCAGCAATACTAACTATAATCT 42539 0.10100449839117943 No Hit CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA 42446 0.10078367941681757 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.00997888068334E-4 0.0 0.0 1.6620783016485015E-5 0.0 2 5.081210807896848E-4 2.3743975737835737E-6 0.0 2.136957816405216E-5 0.0 3 5.104954783634683E-4 2.3743975737835737E-6 0.0 2.6118373311619308E-5 0.0 4 5.152442735110354E-4 2.3743975737835737E-6 0.0 4.036475875432075E-5 0.0 5 5.223674662323862E-4 2.3743975737835737E-6 0.0 4.748795147567147E-5 0.0 6 5.34239454101304E-4 2.3743975737835737E-6 0.0 7.360632478729078E-5 0.0 7 5.389882492488712E-4 2.3743975737835737E-6 0.0 1.1634548111539511E-4 0.0 8 5.413626468226548E-4 2.3743975737835737E-6 0.0 1.3059186655809654E-4 0.0 9 5.437370443964383E-4 2.3743975737835737E-6 0.0 1.44838252000798E-4 0.0 10-11 5.484858395440055E-4 4.7487951475671475E-6 0.0 1.5314864350904052E-4 0.0 12-13 5.651066225604905E-4 4.7487951475671475E-6 0.0 1.6502063137795837E-4 0.0 14-15 5.912249958721098E-4 4.7487951475671475E-6 0.0 1.9113900468957767E-4 0.0 16-17 6.161561703968373E-4 4.7487951475671475E-6 0.0 2.1013418527984627E-4 0.0 18-19 6.672057182331842E-4 4.7487951475671475E-6 0.0 2.73055720985111E-4 0.0 20-21 7.004472842661542E-4 4.7487951475671475E-6 0.0 3.4547484698550996E-4 0.0 22-23 7.633688199714189E-4 4.7487951475671475E-6 0.0 3.8346520816604716E-4 0.0 24-25 8.725911083654633E-4 4.7487951475671475E-6 0.0 4.938746953469833E-4 0.0 26-27 0.0010779764984977422 4.7487951475671475E-6 0.0 6.968856879054788E-4 0.0 28-29 0.0014768752908933826 4.7487951475671475E-6 0.0 7.882999944961464E-4 0.0 30-31 0.0023292840198816857 4.7487951475671475E-6 0.0 8.286647532504672E-4 0.0 32-33 0.004159944549268821 4.7487951475671475E-6 0.0 8.46472735053844E-4 0.0 34-35 0.007170680672826392 4.7487951475671475E-6 0.0 8.725911083654633E-4 0.0 36-37 0.011881485459213002 4.7487951475671475E-6 0.0 0.0010316757458089627 0.0 38-39 0.01909609248715439 4.7487951475671475E-6 0.0 0.001081538094858418 0.0 40-41 0.029224085338128224 4.7487951475671475E-6 0.0 0.0011385236366292235 0.0 42-43 0.042474410998627456 4.7487951475671475E-6 0.0 0.0012334995395805665 0.0 44-45 0.0596543646437385 4.7487951475671475E-6 0.0 0.0013011698704333983 0.0 46-47 0.08137179205234996 4.7487951475671475E-6 0.0 0.0013830865867289317 0.0 48-49 0.10876640405987795 4.7487951475671475E-6 0.0 0.0013985201709585248 0.0 50-51 0.14036251257421595 4.7487951475671475E-6 0.0 0.0014139537551881181 0.0 52-53 0.18193583969359253 4.7487951475671475E-6 0.0 0.0014293873394177112 0.0 54-55 0.23738514623416032 4.7487951475671475E-6 0.0 0.0014566929115162224 0.0 56-57 0.2998662311894882 4.7487951475671475E-6 0.0 0.001492308875122976 0.0 58-59 0.36958804154606906 7.123192721350721E-6 0.0 0.001546920019319998 0.0 60-61 0.45043153013825216 7.123192721350721E-6 0.0 0.0015837231817136437 0.0 62-63 0.554700825193384 8.310391508242507E-6 0.0 0.0016229007416810725 0.0 64-65 0.6741318359559109 9.497590295134295E-6 0.0 0.0016537679101402592 0.0 66-67 0.8083684027897652 9.497590295134295E-6 0.0 0.0016668270967960686 0.0 68-69 0.9636551913139977 9.497590295134295E-6 0.0 0.0016834478798125538 0.0 70-71 1.135635181980716 9.497590295134295E-6 0.0 0.0017629901985343035 0.0 72-73 1.3364474823858878 9.497590295134295E-6 0.0 0.0018235373366657845 0.0 74-75 1.5861474416364767 9.497590295134295E-6 0.0 0.001998055558338877 0.0 76-77 1.86506911182762 9.497590295134295E-6 0.0 0.0021464554067003507 0.0 78-79 2.1874173264444776 9.497590295134295E-6 0.0 0.0021737609787988617 0.0 80-81 2.556972126424514 9.497590295134295E-6 0.0 0.0022248105266352083 0.0 82-83 2.984495468770902 9.497590295134295E-6 0.0 0.0022794216708322303 2.3743975737835737E-6 84-85 3.52431000837545 9.497590295134295E-6 0.0 0.002294855255061824 2.3743975737835737E-6 86-87 4.110320827175531 1.1871987868917868E-5 0.0 0.002311476038078309 2.3743975737835737E-6 88-89 4.755073054752231 1.1871987868917868E-5 0.0 0.0023304712186685774 2.3743975737835737E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCGAAA 29985 0.0 48.129177 1 CCGCAAA 10015 0.0 42.2843 1 CTCCGCT 2830 0.0 42.20145 1 CGTCTTC 48395 0.0 42.13706 52-53 GCCGTCT 48695 0.0 42.032112 50-51 TGCCGTC 48265 0.0 41.948963 50-51 ATGCCGT 48945 0.0 41.890385 48-49 TATGCCG 49020 0.0 41.840828 48-49 CCGTCTT 49815 0.0 40.797657 52-53 TCTCGTA 48100 0.0 39.18402 42-43 CCGAAAC 51360 0.0 38.26178 1 TCCGCTC 2715 0.0 37.978775 2 CTCGTAT 51190 0.0 37.737377 44-45 GTATGCC 55175 0.0 37.676506 46-47 GCTTGAA 56070 0.0 36.484882 60-61 CGGCAAT 10700 0.0 36.464493 1 TGCTTGA 56595 0.0 35.78132 60-61 ATCTCGT 52375 0.0 35.59574 42-43 TACGCAT 5320 0.0 35.1719 9 CTACGCA 5350 0.0 34.17556 8 >>END_MODULE