FastQCFastQC Report
Fri 27 May 2016
SRR1233122_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233122_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31499298
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTAT14317924.5454727276779305No Hit
CTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAAT12314143.909337916038637No Hit
CGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCAC9957783.1612704511700547No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT7938102.5200879079908383No Hit
CGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGG6304582.0014985730793113No Hit
ATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTAT5040321.6001372475031033No Hit
ACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAA2566070.8146435517388356No Hit
ACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTA2405910.7637979741643766No Hit
GACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACT2339890.7428387769149649No Hit
TATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGA2067870.6564812968212815No Hit
CACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAG1906870.6053690466371663No Hit
GACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGA1882760.597714907805247No Hit
CTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATAGAT1826880.5799748299152572No Hit
GGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCAC1722070.5467010725127907No Hit
CACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATAC1696920.5387167675927255No Hit
TCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATA1584260.5029508911595427No Hit
TAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTC1454170.4616515580759927No Hit
CGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATA1420370.450921160211253No Hit
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA1344930.4269714201249818No Hit
AGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATA1328540.4217681295627604No Hit
TATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGAC1206840.38313234790184847No Hit
ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT1194450.37919892690941875No Hit
ACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCA1161150.36862726274090296No Hit
AGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAA1087470.3452362652653402No Hit
AGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCA1066670.33863294350242346No Hit
GATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATAC1024590.32527391562821495No Hit
CTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAG1003890.318702340604543No Hit
TACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTC916650.2910064852873864No Hit
AATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGAC892750.2834190146078811No Hit
TAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATA862150.27370451239897475No Hit
CTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGA839950.2666567362866309No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT827380.2626661711635605No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT776460.2465007315401124No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT766620.2433768523984249No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA734620.2332178958400914No Hit
ACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAG686660.21799215969828917No Hit
CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG668480.21222060250358593No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA640910.2034680264937968No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC601580.19098203394882005No Hit
GAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACT591980.18793434698132003No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT579820.1840739434891533No Hit
ACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATAGA549410.17441976008481205No Hit
GAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAAT539590.17130223029097347No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA530380.1683783556065281No Hit
CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT524890.16663545962198903No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT518860.1647211312455281No Hit
AATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTA512830.16280680286906712No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA506160.1606892953614395No Hit
TACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATA498150.15814638154793165No Hit
ATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACT484350.15376533153215036No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA474030.15048906804208778No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG472090.14987318130073882No Hit
TAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGA467520.14842235531725181No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA441910.14029201539666059No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG424020.13461252374576727No Hit
TCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATA405260.1286568354634443No Hit
GACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATAG400530.12715521469716565No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA394410.12521231425538437No Hit
CTCACTATAGGAATACGACTCACTATAGAGATAATACGACTCACTATAGA344380.1093294206112149No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT333020.1057229910330065No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG325980.10348802059017316No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT126500.074.894691
GCGTGTT153600.061.062982
CGAACGT25200.055.5930021
TCGAACG10300.049.7953342
CGTGTTT197400.047.202133
GATCGGG16350.038.340241
GTAGATA183750.037.6818582
CTTACGG34800.035.480751
GTGTTTT273900.033.776254
TTACGGT36850.033.5071872
GATATAG216900.032.9975785
CTCAGTT180650.032.1767461
GAGCGGC24600.031.5498149
GTGGTAC122700.030.421221
TAAACGC26700.030.4149422
GTAAACG27050.030.3722881
TAGATAT236300.029.6842773
ACTCCCG134800.029.0163356
TCATCAA251250.028.9192812
GGTACTC131400.028.8053153
Produced by FastQC (version 0.11.5)