##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233122_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31499298 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.32282671823353 33.0 31.0 34.0 28.0 34.0 2 31.651337340914708 33.0 31.0 34.0 28.0 34.0 3 31.660017248638365 34.0 31.0 34.0 28.0 34.0 4 35.17201046829678 37.0 35.0 37.0 32.0 37.0 5 35.39955376148383 37.0 35.0 37.0 33.0 37.0 6 35.1557297245164 37.0 35.0 37.0 32.0 37.0 7 35.36500426136481 37.0 35.0 37.0 33.0 37.0 8 35.42707599388405 37.0 35.0 37.0 33.0 37.0 9 37.101147047785 39.0 37.0 39.0 33.0 39.0 10-11 37.09122965216558 39.0 37.0 39.0 33.0 39.0 12-13 36.83709525844036 39.0 37.0 39.0 33.0 39.0 14-15 38.001627401347164 40.0 38.0 41.0 33.0 41.0 16-17 38.09570292645887 40.0 38.0 41.0 33.0 41.0 18-19 38.12415195729123 40.0 38.0 41.0 33.0 41.0 20-21 38.029615517145814 40.0 38.0 41.0 33.0 41.0 22-23 37.909931008621214 40.0 38.0 41.0 32.5 41.0 24-25 37.86280956166071 40.0 38.0 41.0 32.5 41.0 26-27 37.79202060312582 40.0 38.0 41.0 32.0 41.0 28-29 37.77283628670074 40.0 38.0 41.0 32.5 41.0 30-31 37.5783797150019 40.0 37.5 41.0 32.0 41.0 32-33 37.42874199926614 40.0 37.0 41.0 31.5 41.0 34-35 37.16597971484951 40.0 37.0 41.0 31.0 41.0 36-37 37.01879726018021 40.0 37.0 41.0 30.0 41.0 38-39 37.03664683257385 40.0 37.0 41.0 30.0 41.0 40-41 36.965824412975806 40.0 37.0 41.0 30.0 41.0 42-43 36.870463414771976 40.0 37.0 41.0 30.0 41.0 44-45 36.74146563520241 40.0 36.0 41.0 30.0 41.0 46-47 36.83297927782391 39.5 36.5 41.0 30.5 41.0 48-49 36.98406791478337 40.0 37.0 41.0 30.5 41.0 50-51 36.93719098120853 40.0 37.0 41.0 30.0 41.0 52-53 36.77224438462089 40.0 37.0 41.0 30.0 41.0 54-55 36.560198135209234 40.0 36.0 41.0 30.0 41.0 56-57 36.44043154549031 39.0 36.0 41.0 29.0 41.0 58-59 36.261357602318625 39.0 36.0 41.0 28.5 41.0 60-61 36.122489602784164 39.0 35.5 41.0 28.0 41.0 62-63 35.945546230903304 39.0 35.0 40.5 28.0 41.0 64-65 35.71720057062859 39.0 35.0 40.0 28.0 41.0 66-67 35.42465430499435 38.0 34.5 40.0 28.0 41.0 68-69 35.04152590956154 37.5 34.0 39.5 28.0 41.0 70-71 34.51155401939434 37.0 34.0 39.0 27.5 40.0 72-73 34.06510311753614 36.5 33.5 39.0 26.0 40.0 74-75 33.52937914679876 36.0 33.0 38.5 26.0 39.5 76-77 32.96306428797239 35.0 32.5 37.0 26.0 39.0 78-79 32.871476865928884 35.0 33.0 37.0 26.0 39.0 80-81 32.58960809221844 35.0 32.5 36.0 26.0 38.5 82-83 32.20807284340114 35.0 32.0 36.0 26.0 37.0 84-85 31.89474340031324 35.0 32.0 35.0 26.0 37.0 86-87 31.582566824187637 35.0 32.0 35.0 25.0 36.0 88-89 31.37239861662949 34.5 32.0 35.0 25.0 36.0 90-91 31.14146867654003 34.0 31.0 35.0 24.5 36.0 92-93 30.870699372411412 34.0 31.0 35.0 23.0 35.5 94-95 30.662874693270943 34.0 31.0 35.0 21.5 35.5 96-97 30.42907576860919 34.0 31.0 35.0 19.5 35.0 98-99 30.14261463858655 34.0 31.0 35.0 16.5 35.0 100-101 29.030008525904293 33.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8599122960606174 1101 2 0.7962986132311798 1101 3 0.8670211492512934 1101 4 0.3311502820908956 1101 5 0.4626386687364814 1101 6 -0.3023367266422241 1101 7 0.327793767618239 1101 8 0.39058370986672486 1101 9 0.5405995280383564 1101 10-11 0.5225633196315584 1101 12-13 0.6470501079604105 1101 14-15 0.8055293760526112 1101 16-17 0.6298079000494994 1101 18-19 0.6241445943150836 1101 20-21 0.5793785298602785 1101 22-23 0.5485328647742307 1101 24-25 0.5523875754565424 1101 26-27 0.5214784328634607 1101 28-29 0.38700463094222215 1101 30-31 0.5392368676104127 1101 32-33 0.4879247009453138 1101 34-35 0.5733339135354285 1101 36-37 0.558841735479163 1101 38-39 0.45186253576747504 1101 40-41 0.3931513763984995 1101 42-43 0.3369046591653273 1101 44-45 0.21775173934075553 1101 46-47 0.36331022209623853 1101 48-49 0.6630341100581418 1101 50-51 0.7281674712596171 1101 52-53 0.7852598942485969 1101 54-55 0.802328178406043 1101 56-57 0.6397977115609947 1101 58-59 0.6532864895186421 1101 60-61 0.7103956912375438 1101 62-63 0.5702046092854545 1101 64-65 0.47013860168797095 1101 66-67 0.4817723761491379 1101 68-69 0.3644352906278172 1101 70-71 0.38348087782988927 1101 72-73 0.3292069536258566 1101 74-75 0.21861115526222363 1101 76-77 0.17858577455896807 1101 78-79 0.2048750640980046 1101 80-81 0.12654708129495162 1101 82-83 0.11161947374065306 1101 84-85 0.12983471212798392 1101 86-87 0.1420997937407371 1101 88-89 0.031071269604211693 1101 90-91 0.02820127000583028 1101 92-93 -0.03566938520205909 1101 94-95 -0.13735892160288188 1101 96-97 -0.21033775481182104 1101 98-99 -0.3826801432119815 1101 100-101 -1.08401932679811 1102 1 0.26141554100150444 1102 2 0.42554265477749453 1102 3 0.6222065615590466 1102 4 -3.041959610754276 1102 5 -0.5310613075183781 1102 6 -1.2696176355113309 1102 7 -0.1166450789702651 1102 8 0.16230961593880977 1102 9 0.3875303377938337 1102 10-11 0.37196280994558606 1102 12-13 0.5258926299695759 1102 14-15 0.7008352868535113 1102 16-17 0.5260996730971144 1102 18-19 0.5144912775872186 1102 20-21 0.4800345597810747 1102 22-23 0.5229291209520142 1102 24-25 0.49222187446802934 1102 26-27 0.4346713213917539 1102 28-29 0.3396814146721354 1102 30-31 0.4820353736904508 1102 32-33 0.4201181499389435 1102 34-35 0.5178518799155611 1102 36-37 0.5309359061380547 1102 38-39 0.37521553720360856 1102 40-41 0.3554003134416135 1102 42-43 0.29643580486855114 1102 44-45 0.20585908160202138 1102 46-47 0.3219066268112414 1102 48-49 0.6363034898250604 1102 50-51 0.6237169833460356 1102 52-53 0.6906025310072721 1102 54-55 0.751450105429484 1102 56-57 0.5999222155219144 1102 58-59 0.603606114269958 1102 60-61 0.602681184795749 1102 62-63 0.4975698390722201 1102 64-65 0.4520062393316806 1102 66-67 0.40591393304745793 1102 68-69 0.3050905101542938 1102 70-71 0.31124451788707574 1102 72-73 0.263632014556606 1102 74-75 0.1746160107318886 1102 76-77 0.16357646206872545 1102 78-79 0.14899147973664384 1102 80-81 0.05448603941233898 1102 82-83 0.07628261629617583 1102 84-85 0.11088418212610662 1102 86-87 0.07431981108621244 1102 88-89 -0.07641340574334876 1102 90-91 -0.12090597365600075 1102 92-93 -0.1870426292305467 1102 94-95 -0.17007274786322668 1102 96-97 -0.23820662799274928 1102 98-99 -0.4045218999176079 1102 100-101 -1.1811595329828748 1103 1 0.862433665510121 1103 2 0.7101354112975784 1103 3 0.8058102801585889 1103 4 0.7631256032142559 1103 5 0.46827457357530733 1103 6 0.6524843377590415 1103 7 0.5366804959072624 1103 8 0.4648391130310543 1103 9 0.46929467182744844 1103 10-11 0.43570996790526806 1103 12-13 0.5758210535885127 1103 14-15 0.6971485167059726 1103 16-17 0.5791534328080488 1103 18-19 0.4141986380285374 1103 20-21 0.42254754879481027 1103 22-23 0.45536261067590544 1103 24-25 0.4933188912900306 1103 26-27 0.43478695155798164 1103 28-29 0.35173902873291496 1103 30-31 0.4782637831780505 1103 32-33 0.45694742289276036 1103 34-35 0.43118911950112704 1103 36-37 0.5010290096262935 1103 38-39 0.2948060933207941 1103 40-41 0.24478105002866357 1103 42-43 0.20421187798479679 1103 44-45 0.13769953836666815 1103 46-47 0.18572948771846143 1103 48-49 0.5135825272574834 1103 50-51 0.6102859998037289 1103 52-53 0.5815854075442743 1103 54-55 0.645899360837177 1103 56-57 0.4044440823097304 1103 58-59 0.4097119351958938 1103 60-61 0.3403162171263503 1103 62-63 0.287260092862482 1103 64-65 0.19811029383011203 1103 66-67 0.2229677325780841 1103 68-69 0.07290180240520527 1103 70-71 0.14391867290626692 1103 72-73 0.08390206646775766 1103 74-75 -0.036490720596923154 1103 76-77 -0.034700950397862584 1103 78-79 -0.019690687561109144 1103 80-81 -0.07274917966794447 1103 82-83 -0.06707194634110891 1103 84-85 -0.0891641850477285 1103 86-87 -0.2092136916180216 1103 88-89 -0.2777522541089894 1103 90-91 -0.2764025371349028 1103 92-93 -0.37935858818653045 1103 94-95 -0.40903652615457986 1103 96-97 -0.4640798453202528 1103 98-99 -0.6440789371262561 1103 100-101 -1.494259092514639 1104 1 0.9003274613382608 1104 2 0.7506510276113829 1104 3 0.8226055765729328 1104 4 0.756962024049308 1104 5 0.5318358697364189 1104 6 0.744324674432157 1104 7 0.5727012440415962 1104 8 0.463881239743543 1104 9 0.5128423288752444 1104 10-11 -0.034163592742913806 1104 12-13 0.4825048298141965 1104 14-15 0.6983459680211794 1104 16-17 0.5420844348860143 1104 18-19 0.4860459493308227 1104 20-21 0.5036557442070659 1104 22-23 0.5448043873174271 1104 24-25 0.5376786730933532 1104 26-27 0.47585463753313917 1104 28-29 0.34114121443253254 1104 30-31 0.48462876639797514 1104 32-33 0.4235344358662587 1104 34-35 0.423057151599302 1104 36-37 0.5511060512531287 1104 38-39 0.41603386746862725 1104 40-41 0.3812304598570577 1104 42-43 0.31729916205615893 1104 44-45 0.26183718355768804 1104 46-47 0.28989380632248185 1104 48-49 0.6582022606543347 1104 50-51 0.6086771293847946 1104 52-53 0.6737622385095108 1104 54-55 0.6956950307801364 1104 56-57 0.6004837988345813 1104 58-59 0.607056901873662 1104 60-61 0.5935315188856336 1104 62-63 0.5313683355005594 1104 64-65 0.48247067957102985 1104 66-67 0.47324719850368524 1104 68-69 0.2966005532901974 1104 70-71 0.2805817693794239 1104 72-73 0.24535246332401073 1104 74-75 0.09924991302942487 1104 76-77 0.11592126252393342 1104 78-79 0.11331775828748647 1104 80-81 0.009982084650474121 1104 82-83 0.039219737092160756 1104 84-85 0.006457128189072847 1104 86-87 -0.03265535688765553 1104 88-89 -0.1567803970575099 1104 90-91 -0.21772487897486315 1104 92-93 -0.2704221445996673 1104 94-95 -0.2770625898968966 1104 96-97 -0.36932432224580225 1104 98-99 -0.5516193060709718 1104 100-101 -1.318515290389751 1105 1 0.5401652950690377 1105 2 -0.7716936022938476 1105 3 0.2815133599217141 1105 4 0.4620536316633732 1105 5 0.3168523896795321 1105 6 0.5976219165205734 1105 7 0.45378953542157063 1105 8 0.44870792964206174 1105 9 0.4322629916920775 1105 10-11 -1.1426208261807034 1105 12-13 0.26225144737905026 1105 14-15 0.40703684048222755 1105 16-17 0.2942088165169068 1105 18-19 0.3521315248081578 1105 20-21 0.3470528075901953 1105 22-23 0.37406924222736393 1105 24-25 0.36672271647650945 1105 26-27 0.328868521881553 1105 28-29 0.24784696733181022 1105 30-31 0.3522400141802109 1105 32-33 0.36657001843574477 1105 34-35 0.40326679300135737 1105 36-37 0.4836833517725694 1105 38-39 0.33483399540439507 1105 40-41 0.32065553355825216 1105 42-43 0.24960043603978477 1105 44-45 0.2009623992352374 1105 46-47 0.29236471481539894 1105 48-49 0.5206504894135122 1105 50-51 0.5653591010917225 1105 52-53 0.6176641075858953 1105 54-55 0.6613268200622997 1105 56-57 0.5310268980296726 1105 58-59 0.49651717797959094 1105 60-61 0.45802406773588444 1105 62-63 0.3481598540224695 1105 64-65 0.335779627027037 1105 66-67 0.340574468478664 1105 68-69 0.2649052273645438 1105 70-71 0.21628222438440048 1105 72-73 0.17324870066025255 1105 74-75 0.06556462082852477 1105 76-77 0.024772053107561476 1105 78-79 0.003175756483216219 1105 80-81 -0.009558405521673308 1105 82-83 -0.04990476645185993 1105 84-85 -0.061853332218824164 1105 86-87 -0.04959311267701594 1105 88-89 -0.19018855654699962 1105 90-91 -0.22144864337876413 1105 92-93 -0.2835816758140872 1105 94-95 -0.3341295926322445 1105 96-97 -0.4221025613946523 1105 98-99 -0.5329626061631423 1105 100-101 -1.2661450400610903 1106 1 0.6859295294980328 1106 2 0.4469363697329456 1106 3 0.614380088414265 1106 4 0.650345932633428 1106 5 0.40767418031053637 1106 6 -3.0306537934024576 1106 7 -0.554326932557025 1106 8 -0.06094965283427456 1106 9 0.24221596252314015 1106 10-11 0.23181110820133455 1106 12-13 0.4240142803483309 1106 14-15 0.5201197580914751 1106 16-17 0.29396916347651825 1106 18-19 0.16781119981020254 1106 20-21 0.2463537130261173 1106 22-23 0.2620273221286453 1106 24-25 0.27757314261972255 1106 26-27 0.34049011338336044 1106 28-29 0.1464337965237732 1106 30-31 0.3634848300006581 1106 32-33 0.473498996454218 1106 34-35 0.36042361060817996 1106 36-37 0.4349459465014931 1106 38-39 0.22923721866089153 1106 40-41 0.2334147457729543 1106 42-43 0.18933793005972177 1106 44-45 0.1959605757338565 1106 46-47 0.2286098598457187 1106 48-49 0.5146698096836388 1106 50-51 0.5672961184956193 1106 52-53 0.6206894091001729 1106 54-55 0.6017297120832978 1106 56-57 0.4907753200013687 1106 58-59 0.42780429868255254 1106 60-61 0.41428597146782664 1106 62-63 0.3084912188937068 1106 64-65 0.23959770439478234 1106 66-67 0.22754275062601437 1106 68-69 0.17477385905434772 1106 70-71 0.16048462244391715 1106 72-73 0.1320124585035245 1106 74-75 0.054113252948368995 1106 76-77 0.011405414977204487 1106 78-79 -0.020376109906891315 1106 80-81 -0.04639161613312126 1106 82-83 -0.0919373210754415 1106 84-85 -0.0854288541355892 1106 86-87 -0.082929363817275 1106 88-89 -0.18049274888506872 1106 90-91 -0.17726395917348015 1106 92-93 -0.2268952194486893 1106 94-95 -0.23285375913459205 1106 96-97 -0.29675770125180634 1106 98-99 -0.4377119775568943 1106 100-101 -1.1906478668008162 1107 1 0.4019822316864037 1107 2 -1.0278627586233817 1107 3 0.15048526983190058 1107 4 0.42825932952833057 1107 5 0.2609891150513022 1107 6 0.5700878508215226 1107 7 0.46953822967965664 1107 8 0.4014738470214567 1107 9 0.5016836765152846 1107 10-11 -0.13741126804829662 1107 12-13 0.4519412746946472 1107 14-15 0.6301248915649111 1107 16-17 0.4438234860225805 1107 18-19 -0.4314699297510032 1107 20-21 0.026870912915128997 1107 22-23 0.255697296378699 1107 24-25 0.3113935532541987 1107 26-27 0.37801758088486537 1107 28-29 0.24192345503156076 1107 30-31 0.34785264085372347 1107 32-33 0.4082983284689661 1107 34-35 0.4387448114211594 1107 36-37 0.5318120429785367 1107 38-39 0.37035298779188963 1107 40-41 0.3728407959535005 1107 42-43 0.3293332021629851 1107 44-45 0.25025125899268374 1107 46-47 0.2586188212140712 1107 48-49 0.5286219565616932 1107 50-51 0.5798948506612192 1107 52-53 0.6380518473608348 1107 54-55 0.6412853100553519 1107 56-57 0.5248408050870808 1107 58-59 0.5800549845189877 1107 60-61 0.5761382379432973 1107 62-63 0.5184173434510484 1107 64-65 0.43924083224844424 1107 66-67 0.44246717745373587 1107 68-69 0.35264253256379163 1107 70-71 0.32643761835104357 1107 72-73 0.30675219868712134 1107 74-75 0.21606009858352593 1107 76-77 0.13062514102988843 1107 78-79 0.095026934959769 1107 80-81 0.04772308995929819 1107 82-83 0.07306589847784295 1107 84-85 0.1043150040736549 1107 86-87 0.04283197163340802 1107 88-89 -0.09363503935180262 1107 90-91 -0.1266384337238975 1107 92-93 -0.16673699351020232 1107 94-95 -0.1650655980349498 1107 96-97 -0.19629802413242103 1107 98-99 -0.31580749294289134 1107 100-101 -0.970403761574488 1108 1 -1.8513535140838009 1108 2 -0.46792474925659633 1108 3 0.0056283199142015405 1108 4 0.1305661670150684 1108 5 -0.028824012857675996 1108 6 0.21383978476237075 1108 7 -0.001853137387321624 1108 8 0.0035267521042001704 1108 9 -0.027746996846381933 1108 10-11 -0.06385942955134283 1108 12-13 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0.8635597080924029 2201 28-29 0.8110868674941756 2201 30-31 0.9972427537068143 2201 32-33 1.1185566084088165 2201 34-35 1.2008532886713468 2201 36-37 1.3076036481908275 2201 38-39 1.3021299072808432 2201 40-41 1.3481267306084632 2201 42-43 1.434988406867646 2201 44-45 1.4498458226034785 2201 46-47 1.3523374838376299 2201 48-49 1.2687988331472013 2201 50-51 1.3342272182052355 2201 52-53 1.3915274726419682 2201 54-55 1.4506271636544739 2201 56-57 1.4117609280901533 2201 58-59 1.4827590858946138 2201 60-61 1.4986570090919926 2201 62-63 1.4901864378103369 2201 64-65 1.423187161777399 2201 66-67 1.425746468802771 2201 68-69 1.3568067526323517 2201 70-71 1.3969773868584525 2201 72-73 1.33614198620225 2201 74-75 1.3877157794214625 2201 76-77 1.3649766639340513 2201 78-79 1.3794732352993364 2201 80-81 1.3215469364789456 2201 82-83 1.3694885880688261 2201 84-85 1.3831283050130345 2201 86-87 1.4470784693525793 2201 88-89 1.4554810958075137 2201 90-91 1.4964409156872804 2201 92-93 1.5544143509726176 2201 94-95 1.6600524076196805 2201 96-97 1.759176629884326 2201 98-99 1.8386842664487197 2201 100-101 2.314751488546868 2202 1 0.2143417616683685 2202 2 -0.5035325704966596 2202 3 -0.7534601997573098 2202 4 0.04391811073496399 2202 5 -0.055610530566362115 2202 6 -1.8405983573328868 2202 7 -0.3129999379398001 2202 8 -0.05368994181579012 2202 9 0.1355788563029634 2202 10-11 -0.9370753963751781 2202 12-13 -0.4236304405566358 2202 14-15 -0.253176411044997 2202 16-17 -0.6404856412777278 2202 18-19 -1.3004459591151516 2202 20-21 -0.035697065853604215 2202 22-23 -0.5749485692117844 2202 24-25 0.2405463186761665 2202 26-27 0.4061396692896011 2202 28-29 0.3617390149399924 2202 30-31 0.5710670415389814 2202 32-33 0.686284516702031 2202 34-35 0.7755885395523237 2202 36-37 0.8654364548495721 2202 38-39 0.8173500965697542 2202 40-41 0.8892694885695036 2202 42-43 0.9928964565872249 2202 44-45 1.0465536595737603 2202 46-47 0.9075512918549293 2202 48-49 0.8046287822197797 2202 50-51 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0.015011811526278507 2215 96-97 -0.009815797147854965 2215 98-99 -0.0561388435576724 2215 100-101 0.1565362945011337 2216 1 -0.5504580501260214 2216 2 -0.29440586311540784 2216 3 -1.2688973923551359 2216 4 -0.24663561790328714 2216 5 -1.6012372148268526 2216 6 -0.1848478728049372 2216 7 -1.0728157981303426 2216 8 -0.4351807944724797 2216 9 -1.4908271809434268 2216 10-11 -0.4698805997477109 2216 12-13 -0.2874776377981476 2216 14-15 -0.3959969301633848 2216 16-17 -0.44393373167842043 2216 18-19 -0.4229855301309229 2216 20-21 -0.33556674883172377 2216 22-23 -0.3243248261933047 2216 24-25 -0.1822770718804918 2216 26-27 -1.2721640827066594 2216 28-29 -0.86366323461354 2216 30-31 -1.2724845290481284 2216 32-33 -2.8535029649680226 2216 34-35 -2.324169820519856 2216 36-37 -1.7399493816994607 2216 38-39 -3.88404834091763 2216 40-41 -2.6012554596364694 2216 42-43 -1.8393208604907585 2216 44-45 -2.5736729924570625 2216 46-47 -1.9556058085051404 2216 48-49 -5.667645262711076 2216 50-51 -5.061784291720148 2216 52-53 -3.0682622667606125 2216 54-55 -11.229870818506939 2216 56-57 -6.221451834892822 2216 58-59 -5.778012525686293 2216 60-61 -4.389608128232062 2216 62-63 -2.8041012428008827 2216 64-65 -2.7376901028595952 2216 66-67 -3.8027831757088073 2216 68-69 -2.4134468167498895 2216 70-71 -3.3787034325286953 2216 72-73 -3.0832279345407088 2216 74-75 -2.519316363270704 2216 76-77 -2.5175740956364336 2216 78-79 -1.4412244840573507 2216 80-81 -1.2461998456501888 2216 82-83 -3.0503409350104427 2216 84-85 -2.1395265506765533 2216 86-87 -1.3037511164965814 2216 88-89 -1.1689676467288166 2216 90-91 -1.3406396629316362 2216 92-93 -2.093427445508876 2216 94-95 -1.9272724136293533 2216 96-97 -1.5163283148592832 2216 98-99 -1.510716271373429 2216 100-101 -1.3912740754954172 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 127068.0 3 34731.0 4 27293.0 5 28920.0 6 42697.0 7 45283.0 8 48365.0 9 57840.0 10 69019.0 11 72573.0 12 72102.0 13 70617.0 14 72130.0 15 75302.0 16 77431.0 17 78606.0 18 81633.0 19 84330.0 20 88386.0 21 96348.0 22 107432.0 23 125900.0 24 153366.0 25 190451.0 26 240061.0 27 302047.0 28 381127.0 29 470762.0 30 599641.0 31 756511.0 32 951553.0 33 1270644.0 34 1745287.0 35 2510676.0 36 3886996.0 37 6530505.0 38 8597601.0 39 1325968.0 40 2096.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.912759718289315 22.326567744266963 15.229892201235327 42.530780336208394 2 20.62171695035975 21.096165044608465 48.233743148043665 10.04837485698812 3 12.139769195086288 31.096576887114306 30.4699205164344 26.293733401365 4 13.735143945275086 36.25488742882768 25.216206778925983 24.793761846971258 5 20.455826681800495 30.145953217518855 26.984186004095733 22.414034096584913 6 12.613160680242338 35.05994469672598 33.485941872174934 18.840952750856747 7 10.637569253130946 34.446137222896176 36.7103850624284 18.20590846154447 8 12.353061606924335 22.497973403161254 44.16321215543368 20.98575283448073 9 13.786049067177364 32.42738100625905 34.605233842838615 19.181336083724972 10-11 23.90871993877402 36.89672561678506 24.56240731155069 14.632147132890228 12-13 22.4757856530605 34.66674039547861 29.37374612635817 13.48372782510272 14-15 17.67019597295143 36.200188003550146 33.30067279064954 12.82894323284888 16-17 20.554426088850352 33.6315007916466 29.998514818952906 15.815558300550142 18-19 17.60523810897893 37.57564769856766 24.43766809728433 20.38144609516908 20-21 20.388406633499002 32.6814241982011 29.228615202585793 17.7015539657141 22-23 18.463013350107595 30.179999447075584 27.970612241838722 23.3863749609781 24-25 15.310222702421198 34.46682140823125 26.38003759809325 23.84291829125431 26-27 17.189690812713692 30.26356577476313 32.0724702056022 20.474273206920977 28-29 21.143334768413713 32.922127670529136 28.151576186910702 17.78296137414645 30-31 20.375769531823597 38.761731313475444 25.764689518682516 15.09780963601844 32-33 19.900370751154608 35.49066007553503 29.30473915450442 15.304230018805942 34-35 21.890711612321148 34.76161646747922 27.057309787509993 16.290362132689637 36-37 19.908822151105916 39.69444013503625 23.07944640021613 17.317291313641704 38-39 18.941435652116382 33.575039607417374 29.84555354353344 17.637971196932803 40-41 19.29126358426945 32.569506531000684 26.057734867008115 22.081495017721746 42-43 16.494689665156493 36.23955460767419 24.975213166136136 22.290542561033185 44-45 18.089927136927034 31.340981490436853 30.662140371166796 19.906951001469317 46-47 21.01042856872704 31.29633968738127 25.883591258181326 21.809640485710364 48-49 17.796175213717625 37.527691492381194 26.817062476802185 17.859070817099 50-51 17.107251748807446 33.41031364734665 32.3576163871774 17.124818216668505 52-53 23.14723587361772 33.370408320779156 25.407097603039986 18.07525820256314 54-55 21.27989240650614 36.52733174532492 24.578700834798685 17.614075013370254 56-57 20.363507318142066 33.71270034522643 29.054046426479413 16.869745910152087 58-59 18.535165256673604 34.26867606772113 27.512616147274628 19.683542528330637 60-61 17.605105991893147 36.74785078450842 24.97248784441872 20.674555379179708 62-63 19.739934089286272 32.526352179764864 29.5118836548495 18.22183007609937 64-65 22.588945231716757 29.72089708919215 27.166966253753944 20.52319142533715 66-67 19.04471864929768 36.22101562265345 23.723350070235043 21.01091565781383 68-69 19.39308624434047 31.180656427953558 29.943863587718 19.482393739987973 70-71 20.38391909022549 31.77454393144936 27.844477512219413 19.997059466105743 72-73 20.863113546589084 35.1681181910108 25.15212154107195 18.816646721328173 74-75 22.230527177645122 31.491151015223252 28.116722679471064 18.161599127660562 76-77 19.708454421661344 33.79486129543229 26.864295308204543 19.632388974701826 78-79 19.56505876584035 36.849808218431676 25.603030681272358 17.98210233445561 80-81 18.57734473105335 33.19218587932338 30.244297004331155 17.98617238529211 82-83 23.148821394227447 31.681246726491985 26.020487496489338 19.149444382791227 84-85 19.47315892094999 37.03961870621424 23.75794317070691 19.72927920212885 86-87 20.142559230052385 33.477834572658175 28.738087232954474 17.641518964334963 88-89 22.791550428997173 29.26009338809019 26.51508459604539 21.433271586867246 90-91 19.387862137485453 34.869880596704505 25.29794939285444 20.444307872955598 92-93 20.308515067623215 30.575889024499155 29.543007174113654 19.57258873376398 94-95 22.5932446585699 33.49717417143025 24.426513406253516 19.483067763746327 96-97 22.787378163629608 33.8011963209689 26.235169131105433 17.176256384296064 98-99 22.03924250827981 32.2671747792983 28.474867484514622 17.218715227907268 100-101 25.397866603280107 32.22291065279973 25.688159534212947 16.691063209707213 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 63.0 1 156.5 2 363.0 3 639.0 4 859.5 5 911.5 6 875.0 7 875.0 8 899.0 9 906.5 10 1007.5 11 1146.5 12 1348.5 13 1675.5 14 2181.0 15 5790.0 16 16612.5 17 20423.0 18 22425.0 19 24448.5 20 23554.5 21 27275.5 22 32395.0 23 46879.0 24 65013.0 25 89019.0 26 117151.5 27 178040.5 28 253346.5 29 376842.5 30 496974.5 31 584114.5 32 1063641.5 33 2252441.0 34 2919561.0 35 2585947.5 36 1832815.0 37 1467122.0 38 1724467.5 39 2568794.0 40 2778525.5 41 1749524.0 42 1077604.5 43 879652.5 44 766452.5 45 602589.0 46 493216.5 47 431181.0 48 401808.5 49 384442.5 50 351535.0 51 321785.5 52 318843.5 53 322256.5 54 309440.0 55 265825.0 56 224686.5 57 210440.0 58 194347.0 59 154787.0 60 109531.0 61 78610.5 62 55579.0 63 39681.0 64 32056.0 65 26121.5 66 19113.0 67 11574.5 68 6005.5 69 3999.0 70 3030.5 71 3499.0 72 6658.5 73 10255.0 74 10263.0 75 7675.0 76 6350.5 77 8756.5 78 7699.5 79 2857.5 80 893.0 81 285.5 82 133.0 83 88.0 84 71.5 85 63.0 86 52.5 87 41.0 88 33.5 89 22.0 90 19.0 91 21.0 92 17.5 93 19.5 94 19.0 95 15.5 96 23.0 97 24.5 98 19.5 99 21.0 100 99.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010466899929007942 2 0.019952825615351808 3 0.01943217909173722 4 0.01742578517146636 5 0.01922582528664607 6 0.03526427795311502 7 0.02750537488168784 8 0.0295720876065238 9 0.04086757742982082 10-11 0.029835585542255575 12-13 0.07488579586757775 14-15 0.12968384247801332 16-17 0.04736930962715423 18-19 0.04797408500976752 20-21 0.03683256687180775 22-23 0.03864689301964761 24-25 0.05096145317270245 26-27 0.05049160143187953 28-29 0.049459832406423786 30-31 0.051928141382706364 32-33 0.04624864973181307 34-35 0.04326763091672709 36-37 0.04624706239485083 38-39 0.04655659310248755 40-41 0.03314518310852515 42-43 0.0247021378063727 44-45 0.027913320480983418 46-47 0.029253032877113644 48-49 0.03368487767568661 50-51 0.0237481482920667 52-53 0.017360704356014535 54-55 0.05427740008682098 56-57 0.025198974275553697 58-59 0.014728899672621275 60-61 0.018876611154953357 62-63 0.018843277078746327 64-65 0.017127365822565317 66-67 0.011505018302312642 68-69 0.016828946473664272 70-71 0.026724405096265953 72-73 0.019497259907189042 74-75 0.019214713927910396 76-77 0.02538310536317349 78-79 0.018784545611143462 80-81 0.015647967773758008 82-83 0.018867087133179923 84-85 0.01870517876303148 86-87 0.019500434581113522 88-89 0.015613046360588733 90-91 0.018478189577431216 92-93 0.018605176534410385 94-95 0.01979091724520337 96-97 0.02062109447645468 98-99 0.019938539582691654 100-101 0.01830199517462262 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3.1499298E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.073000941889621 #Duplication Level Percentage of deduplicated Percentage of total 1 76.34418132935039 9.980475544263898 2 9.339565221283923 2.441922898693685 3 3.9713835892513107 1.5575370420866212 4 2.2078897708835385 1.1545498021739586 5 1.4313395610742727 0.9355951715043921 6 0.9831076739197747 0.7711300528279278 7 0.7371224817269196 0.6745482028532825 8 0.5761416643820862 0.6025520416903094 9 0.452344582672955 0.5322151039807989 >10 3.1632662891155254 8.136439298904593 >50 0.376963282982903 3.457409493900234 >100 0.3201013661470974 8.690678960032823 >500 0.048594069221842624 4.399050332487669 >1k 0.03875300646824136 10.07426453527365 >5k 0.00482515655792454 4.282372801100155 >10k+ 0.004420954961449185 42.30925871822596 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTAT 1431792 4.5454727276779305 No Hit CTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAAT 1231414 3.909337916038637 No Hit CGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCAC 995778 3.1612704511700547 No Hit ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT 793810 2.5200879079908383 No Hit CGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGG 630458 2.0014985730793113 No Hit ATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTAT 504032 1.6001372475031033 No Hit ACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAA 256607 0.8146435517388356 No Hit ACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTA 240591 0.7637979741643766 No Hit GACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACT 233989 0.7428387769149649 No Hit TATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGA 206787 0.6564812968212815 No Hit CACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAG 190687 0.6053690466371663 No Hit GACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGA 188276 0.597714907805247 No Hit CTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATAGAT 182688 0.5799748299152572 No Hit GGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCAC 172207 0.5467010725127907 No Hit CACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATAC 169692 0.5387167675927255 No Hit TCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATA 158426 0.5029508911595427 No Hit TAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTC 145417 0.4616515580759927 No Hit CGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATA 142037 0.450921160211253 No Hit ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA 134493 0.4269714201249818 No Hit AGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATA 132854 0.4217681295627604 No Hit TATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGAC 120684 0.38313234790184847 No Hit ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT 119445 0.37919892690941875 No Hit ACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCA 116115 0.36862726274090296 No Hit AGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAA 108747 0.3452362652653402 No Hit AGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCA 106667 0.33863294350242346 No Hit GATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATAC 102459 0.32527391562821495 No Hit CTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAG 100389 0.318702340604543 No Hit TACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTC 91665 0.2910064852873864 No Hit AATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGAC 89275 0.2834190146078811 No Hit TAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATA 86215 0.27370451239897475 No Hit CTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGA 83995 0.2666567362866309 No Hit CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT 82738 0.2626661711635605 No Hit CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT 77646 0.2465007315401124 No Hit AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT 76662 0.2433768523984249 No Hit CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA 73462 0.2332178958400914 No Hit ACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAG 68666 0.21799215969828917 No Hit CTCACTATAGGGTAATACGACTCACTATAGGGTAATACGACTCACTATAG 66848 0.21222060250358593 No Hit CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA 64091 0.2034680264937968 No Hit TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC 60158 0.19098203394882005 No Hit GAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACT 59198 0.18793434698132003 No Hit CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT 57982 0.1840739434891533 No Hit ACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATAGA 54941 0.17441976008481205 No Hit GAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAAT 53959 0.17130223029097347 No Hit TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA 53038 0.1683783556065281 No Hit CTCACTATAGGTAATACGACTCACTATAGGTAATACGACTCACTATAGGT 52489 0.16663545962198903 No Hit CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT 51886 0.1647211312455281 No Hit AATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTA 51283 0.16280680286906712 No Hit TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA 50616 0.1606892953614395 No Hit TACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATA 49815 0.15814638154793165 No Hit ATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACT 48435 0.15376533153215036 No Hit CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA 47403 0.15048906804208778 No Hit AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG 47209 0.14987318130073882 No Hit TAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGA 46752 0.14842235531725181 No Hit CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA 44191 0.14029201539666059 No Hit CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG 42402 0.13461252374576727 No Hit TCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATA 40526 0.1286568354634443 No Hit GACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATAG 40053 0.12715521469716565 No Hit GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA 39441 0.12521231425538437 No Hit CTCACTATAGGAATACGACTCACTATAGAGATAATACGACTCACTATAGA 34438 0.1093294206112149 No Hit GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT 33302 0.1057229910330065 No Hit TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG 32598 0.10348802059017316 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.8572065320312852E-5 0.0 0.0 6.349347848958412E-6 0.0 2 2.8572065320312852E-5 0.0 0.0 6.349347848958412E-6 0.0 3 3.809608709375047E-5 0.0 0.0 9.524021773437617E-6 0.0 4 4.444543494270888E-5 0.0 0.0 9.524021773437617E-6 0.0 5 4.444543494270888E-5 0.0 0.0 1.2698695697916824E-5 0.0 6 4.7620108867188086E-5 0.0 0.0 1.587336962239603E-5 0.0 7 5.7144130640625705E-5 0.0 0.0 1.587336962239603E-5 0.0 8 5.7144130640625705E-5 0.0 0.0 1.587336962239603E-5 0.0 9 7.619217418750094E-5 0.0 0.0 1.587336962239603E-5 0.0 10-11 1.5714635926172068E-4 0.0 0.0 1.7460706584635632E-5 0.0 12-13 4.2540630588021355E-4 0.0 0.0 1.9048043546875234E-5 0.0 14-15 8.682733183450629E-4 0.0 0.0 1.9048043546875234E-5 0.0 16-17 0.0015619395708437694 0.0 0.0 1.9048043546875234E-5 0.0 18-19 0.0027048221836562833 0.0 0.0 1.9048043546875234E-5 0.0 20-21 0.003920722296731819 0.0 0.0 1.9048043546875234E-5 0.0 22-23 0.005587426107083402 0.0 0.0 2.063538050911484E-5 0.0 24-25 0.007665250190655042 1.587336962239603E-6 0.0 2.698472835807325E-5 0.0 26-27 0.010504996016101692 3.174673924479206E-6 0.0 3.333407620703166E-5 0.0 28-29 0.014652707498433774 3.174673924479206E-6 0.0 3.809608709375047E-5 0.0 30-31 0.022325894373900014 6.349347848958412E-6 0.0 4.444543494270888E-5 0.0 32-33 0.03402139311168141 6.349347848958412E-6 0.0 4.444543494270888E-5 0.0 34-35 0.04786614609633523 6.349347848958412E-6 0.0 4.7620108867188086E-5 0.0 36-37 0.06670307382723259 6.349347848958412E-6 0.0 5.23821197539069E-5 0.0 38-39 0.09014804075951152 6.349347848958412E-6 0.0 5.39694567161465E-5 0.0 40-41 0.11335331981049229 7.936684811198015E-6 0.0 5.39694567161465E-5 0.0 42-43 0.13678400071011107 9.524021773437617E-6 0.0 6.031880456510491E-5 0.0 44-45 0.16567035874894737 9.524021773437617E-6 0.0 6.031880456510491E-5 0.0 46-47 0.19811235158320034 9.524021773437617E-6 0.0 6.031880456510491E-5 0.0 48-49 0.2456181721891072 9.524021773437617E-6 0.0 6.031880456510491E-5 0.0 50-51 0.2981383902587289 9.524021773437617E-6 0.0 6.508081545182372E-5 0.0 52-53 0.3624588713056399 9.524021773437617E-6 0.0 6.666815241406332E-5 0.0 54-55 0.45392757641773473 9.524021773437617E-6 0.0 7.301750026302173E-5 0.0 56-57 0.5643490848589705 9.524021773437617E-6 0.0 7.619217418750094E-5 0.0 58-59 0.6805373884840227 9.524021773437617E-6 0.0 7.777951114974055E-5 0.0 60-61 0.8037052127320425 9.524021773437617E-6 0.0 8.254152203645936E-5 0.0 62-63 0.9377320091387433 9.524021773437617E-6 0.0 8.889086988541776E-5 0.0 64-65 1.0940085077451567 9.524021773437617E-6 0.0 8.889086988541776E-5 0.0 66-67 1.28061266635212 9.524021773437617E-6 0.0 8.889086988541776E-5 0.0 68-69 1.478740891304943 9.524021773437617E-6 0.0 8.889086988541776E-5 0.0 70-71 1.683781968728319 9.524021773437617E-6 0.0 9.047820684765737E-5 0.0 72-73 1.932385921743399 9.524021773437617E-6 0.0 9.682755469661577E-5 0.0 74-75 2.2632250407612258 9.524021773437617E-6 0.0 1.047642395078138E-4 0.0 76-77 2.620247282971195 9.524021773437617E-6 0.0 1.07938913432293E-4 0.0 78-79 2.9947207077440265 9.524021773437617E-6 0.0 1.07938913432293E-4 0.0 80-81 3.4020869290483873 9.524021773437617E-6 0.0 1.1746293520573061E-4 0.0 82-83 3.86930210317703 9.524021773437617E-6 0.0 1.2222494609244943E-4 0.0 84-85 4.44186565681559 9.524021773437617E-6 0.0 1.3809831571484545E-4 0.0 86-87 5.0573301665326 9.524021773437617E-6 0.0 1.4444766356380386E-4 0.0 88-89 5.682707595578797 9.524021773437617E-6 0.0 1.555590222994811E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 12650 0.0 74.89469 1 GCGTGTT 15360 0.0 61.06298 2 CGAACGT 2520 0.0 55.593002 1 TCGAACG 1030 0.0 49.795334 2 CGTGTTT 19740 0.0 47.20213 3 GATCGGG 1635 0.0 38.34024 1 GTAGATA 18375 0.0 37.681858 2 CTTACGG 3480 0.0 35.48075 1 GTGTTTT 27390 0.0 33.77625 4 TTACGGT 3685 0.0 33.507187 2 GATATAG 21690 0.0 32.997578 5 CTCAGTT 18065 0.0 32.176746 1 GAGCGGC 2460 0.0 31.549814 9 GTGGTAC 12270 0.0 30.42122 1 TAAACGC 2670 0.0 30.414942 2 GTAAACG 2705 0.0 30.372288 1 TAGATAT 23630 0.0 29.684277 3 ACTCCCG 13480 0.0 29.016335 6 TCATCAA 25125 0.0 28.919281 2 GGTACTC 13140 0.0 28.805315 3 >>END_MODULE