FastQCFastQC Report
Fri 27 May 2016
SRR1233122_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233122_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31499298
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAG9185802.916191973548109No Hit
CTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTG7489852.377783149326058No Hit
GTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATA7415532.3541889727193284No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT6474422.055417235012666No Hit
CCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCG5650731.7939225185272383No Hit
CTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGT5540661.7589788826404957No Hit
TATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTA5514131.7505564727188523No Hit
CGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGT3936661.2497611851540311No Hit
CTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTC3632781.153289193936957No Hit
ATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTC3370531.0700333702674898No Hit
GTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATC2816090.8940167491986647No Hit
GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT2241740.7116793523462015No Hit
GTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTG2128180.6756277552598157No Hit
TCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGT1984360.6299695948779557No Hit
ATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAG1917360.608699279583945No Hit
ATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTAT1695620.538304059982543No Hit
TATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGA1404030.445733743018654No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC1252510.397631083714945No Hit
CGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTAT1231470.3909515697778408No Hit
GTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCG1149440.36490971957533785No Hit
ATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAG1013750.3218325690940795No Hit
AGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTC977280.3102545332915038No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA954620.30306072217863395No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT877160.278469697959618No Hit
GTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCC852630.2706822228228705No Hit
TTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGT815220.25880576767139385No Hit
TCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATA765530.24303081294065665No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT757950.2406244101059014No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT734080.23304646344816954No Hit
ATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGT659280.20929990249306507No Hit
AGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTC654580.20780780574855984No Hit
GTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATT647390.20552521519685932No Hit
GTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATA645630.204966472586151No Hit
TCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAG645440.20490615378158586No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC625920.19870919028100245No Hit
TCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGT591750.18786132948105702No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA586420.1861692282793096No Hit
ATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAG571230.18134689858802566No Hit
AGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTAT529230.168013268105213No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA529130.16798152136596822No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT488720.15515266403714775No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA483340.15344468946577794No Hit
CTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTCTATAGTGAG463670.14720010585632734No Hit
CTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTC461740.14658739378890284No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC461150.14640008802735857No Hit
GTCGTATTACCCTATAGTGAGTCGTATTACCCTATAGTGAGTCGTATTAC455470.14459687323825438No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT442140.14036503289692362No Hit
ATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTAT424020.13461252374576727No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG400330.12709172121867604No Hit
CCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGT399660.12687901806573593No Hit
CGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTAT398920.1266440921953245No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT387540.12303131326926715No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC365840.11614227085314727No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG361390.11472954095675403No Hit
GAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTA348860.11075167452938159No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC347810.11041833376731126No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC332960.10570394298945963No Hit
AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA331950.10538330092308724No Hit
TATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCT326450.10363723026462367No Hit
TATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGA323500.1027007014569023No Hit
GTCGTATTACCTATAGTGAGTCGTATTACCTATAGTGAGTCGTATTACCT315330.1001069928606028No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA102900.050.263351
TCCGCTC15650.042.202812
TACGCAT78200.041.7819679
CTCCGCT18600.041.4418681
CGGCAAT69250.041.157061
CTACGCA80550.040.798838
CTCTACG79600.040.4111636
CCGCAAA41350.037.7429281
TAACGGC21300.036.7884529
TTCGTAT152700.036.6753851
TCGTACC58200.035.8411942
CTCGTAC59300.035.0642051
CGTACCA61450.034.177473
ACTCGTC162900.033.298642-43
TCCACTC442000.033.1752782
CCGAAAC176300.033.007411
GGCAATC87000.032.6058852
CCGCTCA18250.032.284823
CATGGAC256100.032.173419
TCGTCTA168200.032.07999444-45
Produced by FastQC (version 0.11.5)