FastQCFastQC Report
Fri 27 May 2016
SRR1233121_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233121_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15595784
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTAT1969321.2627258751467705No Hit
CTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAAT1449270.9292703720441371No Hit
CGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCAC1442140.9246986236793227No Hit
ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT1097640.703805592588356No Hit
ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA1031530.6614159313824813No Hit
CGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGG750630.4813031521852316No Hit
AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT636190.407924346733707No Hit
CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT607300.38940010967066485No Hit
ATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTAT602200.38612999513201773No Hit
CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT551260.3534673216812954No Hit
CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC544590.3491905248238883No Hit
CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA507100.32515197697018633No Hit
TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC464220.2976573668883847No Hit
CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT413750.26529605693436126No Hit
TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA408350.2618335827169702No Hit
AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG403420.25867247199627796No Hit
TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA399690.2562807999905616No Hit
CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA380780.24415572824040138No Hit
ACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTA369000.23660240485505568No Hit
GACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACT366580.23505070344652118No Hit
CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT352390.22595209064193247No Hit
CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA344450.2208609711445093No Hit
CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA338520.2170586614946706No Hit
ACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAA330060.21163411855409128No Hit
TATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGA324070.2077933369685038No Hit
CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA300530.19269951417639536No Hit
CTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATAGAT286130.18346624959668587No Hit
CACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAG276120.17704784831592948No Hit
CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG262570.1683596028259945No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT262010.16800053142567248No Hit
TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG259050.16610258259539887No Hit
GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA258240.1655832114627902No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT252140.1616718979949966No Hit
GTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTG242070.15521502477849142No Hit
GACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGA237850.15250916529749323No Hit
TCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATA232870.14931599463034367No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT231000.14811695263283975No Hit
GGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCAC229580.1472064501534517No Hit
CGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATA227320.14575734057358064No Hit
ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC215600.13824248912398376No Hit
CAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTGTGTAGAAAT215100.13792188965941052No Hit
CACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATAC209330.13422217183823526No Hit
AGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATA192460.12340514590353394No Hit
ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT191260.12263570718855814No Hit
CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC188070.12059028260458084No Hit
GAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGT186750.11974390001810746No Hit
TAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTC185960.11923735286408174No Hit
ACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCA183240.11749329177680326No Hit
ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG179580.1151465036961271No Hit
CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA179220.11491567208163436No Hit
AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA177920.11408211347374393No Hit
GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG177470.113793573955628No Hit
GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT174630.11197256899685196No Hit
CGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAG171730.11011309210232714No Hit
GTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCT171490.10995920435933199No Hit
TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA170580.10937571333380867No Hit
TATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGAC164090.10521433228364793No Hit
GTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGT162730.10434230174000872No Hit
AGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAA162320.10407941017905864No Hit
CCCAATTCAGGTTAAGATAAGTAGGTTGGCTACTAGGATTCAGTACAAAA161420.10350233114282681No Hit
CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT158140.10139919865522631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT93600.080.366921
GCGTGTT115000.063.0997542
GATCGGG12000.053.821481
CGTGTTT145200.049.3233073
TCGAACG8150.048.9467472
CGAACGT15800.048.391241
ATCGGGA14400.044.5218732
GAGCGGC16850.043.234779
GTAGATA141650.039.0246052
TAGATAT152550.036.3931583
CTTACGG32900.035.6532441
GATATAG161500.035.3778535
TTACGGT33700.034.384452
AGATATA169800.033.815394
GTAAACG14150.033.5615121
GTCGAAC12300.033.2040671
GCCGGAT13950.033.0219346-47
TCGGGGG27400.032.84804538-39
CGCCGGA10900.032.02327746-47
GTGTTTT222150.031.896894
Produced by FastQC (version 0.11.5)