##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1233121_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15595784 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.961170531728317 31.0 31.0 34.0 27.0 34.0 2 31.23529089656538 31.0 31.0 34.0 28.0 34.0 3 31.374913374024672 33.0 31.0 34.0 28.0 34.0 4 34.94039696882183 37.0 35.0 37.0 32.0 37.0 5 35.18469241430889 37.0 35.0 37.0 32.0 37.0 6 34.937696623651625 37.0 35.0 37.0 32.0 37.0 7 35.13754056865625 37.0 35.0 37.0 32.0 37.0 8 35.19474583643888 37.0 35.0 37.0 32.0 37.0 9 36.81975936573628 39.0 37.0 39.0 33.0 39.0 10-11 36.822032480059995 39.0 37.0 39.0 33.0 39.0 12-13 36.57178882446692 39.0 37.0 39.0 32.0 39.0 14-15 37.65316870251601 40.0 37.0 41.0 32.0 41.0 16-17 37.73581017151815 40.0 37.0 41.0 32.0 41.0 18-19 37.75108420326929 40.0 37.5 41.0 32.0 41.0 20-21 37.63675173367366 40.0 37.0 41.0 32.0 41.0 22-23 37.47606548667255 40.0 37.0 41.0 32.0 41.0 24-25 37.3959910896432 40.0 37.0 41.0 32.0 41.0 26-27 37.289040935678514 40.0 37.0 41.0 31.5 41.0 28-29 37.24970690796948 40.0 37.0 41.0 31.5 41.0 30-31 37.049975813976396 40.0 37.0 41.0 31.0 41.0 32-33 36.96160702789933 40.0 37.0 41.0 30.5 41.0 34-35 36.69482492832678 39.5 36.0 41.0 30.0 41.0 36-37 36.5308479843014 39.0 36.0 41.0 30.0 41.0 38-39 36.615233899110166 39.0 36.0 41.0 30.0 41.0 40-41 36.55962576168021 39.0 36.0 41.0 30.0 41.0 42-43 36.419245034427256 39.0 36.0 41.0 30.0 41.0 44-45 36.31862402685239 39.0 36.0 41.0 30.0 41.0 46-47 36.383517590394945 39.0 35.5 40.5 30.0 41.0 48-49 36.53095567366155 40.0 36.0 41.0 30.0 41.0 50-51 36.49803292992516 40.0 36.0 41.0 30.0 41.0 52-53 36.34867118575123 39.0 36.0 41.0 29.5 41.0 54-55 36.16721034992534 39.0 35.5 41.0 29.0 41.0 56-57 36.09916673634362 39.0 35.0 41.0 28.5 41.0 58-59 35.91572684002291 39.0 35.0 41.0 28.0 41.0 60-61 35.768483969770294 39.0 35.0 40.0 28.0 41.0 62-63 35.59307085812422 39.0 35.0 40.0 28.0 41.0 64-65 35.35864590071265 38.0 34.0 40.0 28.0 41.0 66-67 35.11636253746525 38.0 34.0 40.0 28.0 41.0 68-69 34.82159386793251 37.5 34.0 40.0 27.5 41.0 70-71 34.4004593805608 37.0 34.0 39.0 26.0 41.0 72-73 34.090264138051666 36.5 33.0 39.0 26.0 41.0 74-75 33.66574238909695 36.0 33.0 39.0 26.0 40.0 76-77 33.261334216990946 35.5 32.5 37.5 26.0 40.0 78-79 33.15531998904319 35.0 33.0 37.0 26.0 39.0 80-81 32.879356626124085 35.0 33.0 37.0 26.0 39.0 82-83 32.49838446723807 35.0 32.5 36.5 26.0 39.0 84-85 32.18285239780187 35.0 32.0 36.0 26.0 37.0 86-87 31.859876008798274 35.0 32.0 36.0 25.5 37.0 88-89 31.63397941392366 35.0 32.0 35.0 25.0 36.5 90-91 31.39256744002097 35.0 32.0 35.0 25.0 36.0 92-93 31.144100033701417 34.5 32.0 35.0 24.0 36.0 94-95 30.894370715829353 34.0 31.0 35.0 23.0 36.0 96-97 30.708279814596047 34.0 31.0 35.0 21.5 36.0 98-99 30.46916544881617 34.0 31.0 35.0 19.5 35.0 100-101 29.48329788999386 34.0 30.0 35.0 9.5 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9536417338418097 1101 2 0.9192282241660195 1101 3 0.9393808160259525 1101 4 0.3988465284784155 1101 5 0.5241211825207799 1101 6 -0.24364470190429444 1101 7 0.36772803528016595 1101 8 0.47822941841695865 1101 9 0.5779331120527118 1101 10-11 0.6134024858947811 1101 12-13 0.7574773791486322 1101 14-15 0.9023384060917792 1101 16-17 0.755884021793058 1101 18-19 0.7337925526145099 1101 20-21 0.6737867368674344 1101 22-23 0.632225095278578 1101 24-25 0.6238861700127458 1101 26-27 0.6180365169491324 1101 28-29 0.508950717446524 1101 30-31 0.629325374943484 1101 32-33 0.5912365969868247 1101 34-35 0.7145153261445927 1101 36-37 0.6978656373489684 1101 38-39 0.6146393716840777 1101 40-41 0.511427142918393 1101 42-43 0.45847526702728914 1101 44-45 0.38165787576367194 1101 46-47 0.48820265021898024 1101 48-49 0.7738743964215331 1101 50-51 0.8668808642625763 1101 52-53 0.9094777890272425 1101 54-55 0.9336094669896084 1101 56-57 0.8139623942744123 1101 58-59 0.7800879691706868 1101 60-61 0.8489713632587552 1101 62-63 0.6817120392152205 1101 64-65 0.5392138663351176 1101 66-67 0.5617551797878377 1101 68-69 0.47650287012603343 1101 70-71 0.4501998960266036 1101 72-73 0.4526871512524835 1101 74-75 0.3485049683630024 1101 76-77 0.3026838653825692 1101 78-79 0.3181386912941804 1101 80-81 0.19173811676475339 1101 82-83 0.18236714588822878 1101 84-85 0.14193070053007517 1101 86-87 0.19633332447891405 1101 88-89 0.0910326188362447 1101 90-91 0.12762648974277724 1101 92-93 0.09516620356452421 1101 94-95 -0.06308400801909642 1101 96-97 -0.057397898571334593 1101 98-99 -0.20691767830122743 1101 100-101 -0.9192008243314795 1102 1 0.2730942807023986 1102 2 0.4430696485180228 1102 3 0.663375705549317 1102 4 -2.9441832897764932 1102 5 -0.4490457683566973 1102 6 -1.3357749374107755 1102 7 -0.11400318224909967 1102 8 0.22558003414027894 1102 9 0.48341519279384926 1102 10-11 0.4656525634326485 1102 12-13 0.5938696548207716 1102 14-15 0.7756251183375937 1102 16-17 0.6175015433975659 1102 18-19 0.6269888154749381 1102 20-21 0.564893004631962 1102 22-23 0.6179318767851498 1102 24-25 0.5687204083285664 1102 26-27 0.5343855745232418 1102 28-29 0.4566799720241548 1102 30-31 0.5793086417434523 1102 32-33 0.5400272232573187 1102 34-35 0.6197340809604199 1102 36-37 0.6646873168587817 1102 38-39 0.5309495857145521 1102 40-41 0.48932904715425707 1102 42-43 0.3885455933072919 1102 44-45 0.3384878425593243 1102 46-47 0.40363199721819853 1102 48-49 0.7066959853944894 1102 50-51 0.7005894350793938 1102 52-53 0.8161953878372827 1102 54-55 0.855889043836207 1102 56-57 0.6935469841278916 1102 58-59 0.7406253028735676 1102 60-61 0.6631516664582975 1102 62-63 0.5589600543990869 1102 64-65 0.47327041359156397 1102 66-67 0.41071460935003756 1102 68-69 0.36779499423560225 1102 70-71 0.3824472294693919 1102 72-73 0.3562435246432827 1102 74-75 0.2549630316019815 1102 76-77 0.24684767720263068 1102 78-79 0.18305867032326262 1102 80-81 0.11415627253734328 1102 82-83 0.1414224611952406 1102 84-85 0.08503273346398998 1102 86-87 0.05430863600649616 1102 88-89 -0.08344615594374716 1102 90-91 -0.12056327466657635 1102 92-93 -0.14132803246837966 1102 94-95 -0.10985331811961885 1102 96-97 -0.17171591606444636 1102 98-99 -0.3200873659898278 1102 100-101 -1.074983081570597 1103 1 0.953134060738126 1103 2 0.7957709244067956 1103 3 0.8752760912212665 1103 4 0.8757074028129423 1103 5 0.5467767577387832 1103 6 0.74800695467151 1103 7 0.6168633134750934 1103 8 0.5715728025606168 1103 9 0.574360069651398 1103 10-11 0.5173033168883308 1103 12-13 0.6778883402836087 1103 14-15 0.7866466293020906 1103 16-17 0.643028325743991 1103 18-19 0.476892361353741 1103 20-21 0.44511374467219156 1103 22-23 0.509056473763998 1103 24-25 0.5557497929743604 1103 26-27 0.4777005139355808 1103 28-29 0.3774841891110441 1103 30-31 0.47824725043357574 1103 32-33 0.5501715726379786 1103 34-35 0.45989087100211634 1103 36-37 0.6002431151364505 1103 38-39 0.4042010698161178 1103 40-41 0.3399417093131163 1103 42-43 0.24415405728090178 1103 44-45 0.1644873494434833 1103 46-47 0.21625266311454538 1103 48-49 0.5079698066963374 1103 50-51 0.6584805736537831 1103 52-53 0.6345050445079323 1103 54-55 0.6676660279925244 1103 56-57 0.5003602259259807 1103 58-59 0.4873427062625382 1103 60-61 0.45623679187023924 1103 62-63 0.3692696652576615 1103 64-65 0.21423518519605267 1103 66-67 0.19306267258686205 1103 68-69 0.1133885715513614 1103 70-71 0.1471259013315489 1103 72-73 0.08285627682771946 1103 74-75 -0.00933832345282326 1103 76-77 0.023890214268362797 1103 78-79 -0.010883554076755786 1103 80-81 -0.06383540894204032 1103 82-83 -0.09937080821917732 1103 84-85 -0.10724206283299509 1103 86-87 -0.2022565388987303 1103 88-89 -0.2602161911258598 1103 90-91 -0.2906092896862873 1103 92-93 -0.36781726923977587 1103 94-95 -0.35920595621574947 1103 96-97 -0.35583741791781875 1103 98-99 -0.5307175657520986 1103 100-101 -1.3971839483245567 1104 1 0.9350848316961802 1104 2 0.794476057549069 1104 3 0.8569217096531361 1104 4 0.8260962079286003 1104 5 0.5976539163131207 1104 6 0.8188354310834711 1104 7 0.6225874639031446 1104 8 0.5042695980585066 1104 9 0.5635184947324845 1104 10-11 -0.03656779131043919 1104 12-13 0.5181223697270525 1104 14-15 0.6899254700684736 1104 16-17 0.590701448377537 1104 18-19 0.570678765606182 1104 20-21 0.5370838633184363 1104 22-23 0.58433888547885 1104 24-25 0.5578448779761871 1104 26-27 0.46379209870117677 1104 28-29 0.33238516100857396 1104 30-31 0.4889001712128689 1104 32-33 0.436494576861314 1104 34-35 0.41763665283772866 1104 36-37 0.535131352436693 1104 38-39 0.4593704122399487 1104 40-41 0.3829115470054205 1104 42-43 0.33404867801216653 1104 44-45 0.26073024858689564 1104 46-47 0.28160369922175477 1104 48-49 0.6110655027216794 1104 50-51 0.5971135763868389 1104 52-53 0.6808528254032922 1104 54-55 0.6899291543307271 1104 56-57 0.6158481699450817 1104 58-59 0.6317280876348477 1104 60-61 0.5710124870244613 1104 62-63 0.5007830571350596 1104 64-65 0.41483501062553785 1104 66-67 0.4007321065566245 1104 68-69 0.2591518867274303 1104 70-71 0.26268486491589016 1104 72-73 0.2396488801439105 1104 74-75 0.1054056021106291 1104 76-77 0.13946211342092596 1104 78-79 0.05471364342200502 1104 80-81 -0.07466853866513645 1104 82-83 -0.013884807929862575 1104 84-85 -0.07184923093262086 1104 86-87 -0.0668762006150665 1104 88-89 -0.17214587522916247 1104 90-91 -0.2374698089870968 1104 92-93 -0.2894050844341365 1104 94-95 -0.3104292958077792 1104 96-97 -0.3383615344556681 1104 98-99 -0.5599171310154993 1104 100-101 -1.3064790633738106 1105 1 0.6570622054724922 1105 2 -0.6572669786854171 1105 3 0.4021235207588454 1105 4 0.5745341937292068 1105 5 0.42932076885410453 1105 6 0.724195864554197 1105 7 0.559244688345963 1105 8 0.543178857083781 1105 9 0.5390968353442247 1105 10-11 -1.0784088336558852 1105 12-13 0.3469503339571318 1105 14-15 0.5417108918308671 1105 16-17 0.45061110626601675 1105 18-19 0.48920668603128803 1105 20-21 0.5365731862416041 1105 22-23 0.5037077266645014 1105 24-25 0.5221410414420617 1105 26-27 0.488785821051934 1105 28-29 0.37924298843599047 1105 30-31 0.4539738016201724 1105 32-33 0.5143455776667949 1105 34-35 0.5630919978751763 1105 36-37 0.6617607106413743 1105 38-39 0.4596404354201695 1105 40-41 0.4596405401781922 1105 42-43 0.38715440189378114 1105 44-45 0.34561780128331065 1105 46-47 0.36830238969768914 1105 48-49 0.6430997400892338 1105 50-51 0.701387687879766 1105 52-53 0.7660036643791841 1105 54-55 0.7913903151177806 1105 56-57 0.7191025009635155 1105 58-59 0.6902552280308214 1105 60-61 0.6177511652834156 1105 62-63 0.5122272810842361 1105 64-65 0.4215178392749124 1105 66-67 0.39637160106858715 1105 68-69 0.3493148699194393 1105 70-71 0.33085183251481 1105 72-73 0.29502868036524177 1105 74-75 0.2178524701267932 1105 76-77 0.2132739500594667 1105 78-79 0.07921798626162513 1105 80-81 0.026144520039430574 1105 82-83 0.049788945253830263 1105 84-85 -0.0011937946951690037 1105 86-87 0.06748511533427504 1105 88-89 -0.06436340635012172 1105 90-91 -0.0741928183439704 1105 92-93 -0.12555111473410108 1105 94-95 -0.2092924088069985 1105 96-97 -0.2254512799097057 1105 98-99 -0.35048219766816047 1105 100-101 -1.1796043806522043 1106 1 0.7551181209700566 1106 2 0.49957373273425887 1106 3 0.66050832505098 1106 4 0.7358188176455585 1106 5 0.45104507801886484 1106 6 -3.2068353488581955 1106 7 -0.6378870575019633 1106 8 -0.07643816220161881 1106 9 0.26946947216661243 1106 10-11 0.27838305227714955 1106 12-13 0.5207135112761492 1106 14-15 0.5681223783826113 1106 16-17 0.3975654867261724 1106 18-19 0.2873766936323108 1106 20-21 0.35507787856563056 1106 22-23 0.3245341170794944 1106 24-25 0.3455608146043403 1106 26-27 0.4151597174125854 1106 28-29 0.2029005773432715 1106 30-31 0.43281533857268784 1106 32-33 0.5652603442908983 1106 34-35 0.4335577532246617 1106 36-37 0.556606486641428 1106 38-39 0.3436401148518087 1106 40-41 0.3604651731968147 1106 42-43 0.28059849583841157 1106 44-45 0.26515015384995166 1106 46-47 0.32028391452951155 1106 48-49 0.5975620706970801 1106 50-51 0.6728227154505717 1106 52-53 0.6932114246842076 1106 54-55 0.6544734343103258 1106 56-57 0.5865513453888127 1106 58-59 0.5165821958451033 1106 60-61 0.48255067858750067 1106 62-63 0.3999233712764436 1106 64-65 0.3108174261175236 1106 66-67 0.24895562592053722 1106 68-69 0.25722581596257044 1106 70-71 0.20935436427929233 1106 72-73 0.20266035300985408 1106 74-75 0.12158886232530364 1106 76-77 0.0899027803301422 1106 78-79 0.03298167757444048 1106 80-81 -0.03472501995395305 1106 82-83 -0.09294734804574745 1106 84-85 -0.04696607002821196 1106 86-87 -0.034212335209872435 1106 88-89 -0.15138343641249463 1106 90-91 -0.1510153840852233 1106 92-93 -0.16817403588781943 1106 94-95 -0.23083083057855447 1106 96-97 -0.20867829718849507 1106 98-99 -0.39910402526139777 1106 100-101 -1.141953002966094 1107 1 0.39958806507059563 1107 2 -1.0017260339579295 1107 3 0.15554322924496944 1107 4 0.4410730159741689 1107 5 0.27527928173298477 1107 6 0.5873932857702471 1107 7 0.4613851549011372 1107 8 0.42945089944434756 1107 9 0.521273735174212 1107 10-11 -0.1685194056769319 1107 12-13 0.45158312075896134 1107 14-15 0.585440287784408 1107 16-17 0.44408817491596864 1107 18-19 -0.4363927375056065 1107 20-21 0.017156144063179113 1107 22-23 0.2111274913501049 1107 24-25 0.2805882616329214 1107 26-27 0.36448042079634746 1107 28-29 0.2367127639210196 1107 30-31 0.3362093241913726 1107 32-33 0.3962986008554523 1107 34-35 0.413178591128073 1107 36-37 0.498258620851189 1107 38-39 0.35682828971137326 1107 40-41 0.36718140515371545 1107 42-43 0.3483117784679095 1107 44-45 0.24001795317347785 1107 46-47 0.22976462609648252 1107 48-49 0.5013109700794729 1107 50-51 0.5580034965444014 1107 52-53 0.6491546762004106 1107 54-55 0.6419466106072278 1107 56-57 0.4960117074084778 1107 58-59 0.584225594148009 1107 60-61 0.5877797058544658 1107 62-63 0.5444671871953517 1107 64-65 0.4302458941104206 1107 66-67 0.38517862080323084 1107 68-69 0.35029086380941976 1107 70-71 0.35112423176410346 1107 72-73 0.2991207421590687 1107 74-75 0.1837873699940431 1107 76-77 0.11410020320420955 1107 78-79 0.06885333536713745 1107 80-81 0.04089967343317369 1107 82-83 0.05950451840275406 1107 84-85 0.05682794375032785 1107 86-87 0.07460967939966068 1107 88-89 -0.06048459942489259 1107 90-91 -0.10423098955333998 1107 92-93 -0.11604469209753887 1107 94-95 -0.16106406352978553 1107 96-97 -0.17990654476186307 1107 98-99 -0.26173253349826453 1107 100-101 -0.9585750467784848 1108 1 -1.922216038056476 1108 2 -0.49836325192076103 1108 3 0.01469051924005882 1108 4 0.18629476635556586 1108 5 0.0031841029533978826 1108 6 0.2406682744417452 1108 7 0.04258973702731339 1108 8 0.056643833680666944 1108 9 -0.004798300426060109 1108 10-11 -0.028529556745255036 1108 12-13 -1.1141629817496082 1108 14-15 -2.130776865889672 1108 16-17 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0.27623798526766663 2215 92-93 0.3030292029160364 2215 94-95 0.29377259563898406 2215 96-97 0.2991297334872378 2215 98-99 0.2833913014063967 2215 100-101 0.5676522788831591 2216 1 -0.610643167395132 2216 2 -0.27166212867505024 2216 3 -1.423478211174217 2216 4 -0.27463304337728545 2216 5 -1.652411275915476 2216 6 -0.22302686527078208 2216 7 -1.1306551640831586 2216 8 -0.4742993022225761 2216 9 -1.5293161470887924 2216 10-11 -0.5291900097680795 2216 12-13 -0.3078662614300285 2216 14-15 -0.42129440595683576 2216 16-17 -0.4498064885211548 2216 18-19 -0.44314049968694036 2216 20-21 -0.33805738651017947 2216 22-23 -0.3015002625273695 2216 24-25 -0.17646315098686216 2216 26-27 -1.256115026203105 2216 28-29 -0.840479648737336 2216 30-31 -1.2733260238173187 2216 32-33 -2.780107922486309 2216 34-35 -2.2386406579047105 2216 36-37 -1.6663388136914392 2216 38-39 -3.8162067064064615 2216 40-41 -2.5175870603688537 2216 42-43 -1.6860168185923214 2216 44-45 -2.3023069450417424 2216 46-47 -1.7518226707900695 2216 48-49 -5.552944379534736 2216 50-51 -4.808700605562784 2216 52-53 -2.830278596417827 2216 54-55 -11.0328045457685 2216 56-57 -5.878723702494693 2216 58-59 -5.366548426467496 2216 60-61 -3.929677575780982 2216 62-63 -2.400675105433706 2216 64-65 -2.253034473963311 2216 66-67 -3.2757853272108797 2216 68-69 -1.8849997048313512 2216 70-71 -2.963903568623831 2216 72-73 -2.690788841991356 2216 74-75 -2.0337659901287353 2216 76-77 -1.9963020193542391 2216 78-79 -0.9187966548134057 2216 80-81 -0.684916121393826 2216 82-83 -2.5929375808736665 2216 84-85 -1.5597349507870604 2216 86-87 -0.6706404333578888 2216 88-89 -0.49689628221448956 2216 90-91 -0.6461881836477481 2216 92-93 -1.3824111112514572 2216 94-95 -1.2305032504621884 2216 96-97 -0.7704505945516367 2216 98-99 -0.7374740674948086 2216 100-101 -0.5030133630245395 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 69123.0 3 21810.0 4 15450.0 5 15995.0 6 29404.0 7 30160.0 8 29096.0 9 34892.0 10 43730.0 11 45820.0 12 42805.0 13 38908.0 14 37494.0 15 37576.0 16 38220.0 17 38106.0 18 38509.0 19 39339.0 20 41001.0 21 44540.0 22 51829.0 23 62610.0 24 78535.0 25 99140.0 26 125078.0 27 157964.0 28 199642.0 29 244672.0 30 311520.0 31 393900.0 32 494674.0 33 654272.0 34 894550.0 35 1274213.0 36 1946026.0 37 3138204.0 38 3764059.0 39 971270.0 40 1648.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.532736120298992 19.07328180481322 19.270290797636967 36.12369127725081 2 18.368129486067858 21.381779445792652 50.43876886136607 9.811322206773418 3 14.669293090854055 24.7473354706432 41.64430272670545 18.939068711797297 4 17.70800072006397 32.75438770545279 32.86806649035318 16.66954508413006 5 20.666682870684053 34.65457641747261 30.32190221140586 14.356838500437478 6 15.79044763313936 37.68532290090104 31.819985380705866 14.704244085253729 7 13.162805977302014 21.36434968863836 49.88423832550985 15.588606008549776 8 14.65096695873949 23.655352316302984 42.072032542966696 19.62164818199083 9 17.18099279898085 26.569226900739345 37.95880036896724 18.290979931312567 10-11 20.84918655731697 34.56104702119522 29.42562867021871 15.164137751269097 12-13 20.722549938151815 26.954384419480153 35.83605292744342 16.487012714924614 14-15 18.7049462265491 30.823120216426002 36.0218865689561 14.450046988068802 16-17 20.587678415263344 30.287451468498404 33.83294658884627 15.291923527391981 18-19 19.61550590909085 31.324089765988123 30.84167193021149 18.218732394709537 20-21 21.346818518628748 30.130754165978196 31.735785975917018 16.786641339476034 22-23 20.90773329156246 30.43142506446759 31.039918669954325 17.620922974015624 24-25 19.645495237600045 30.941943283972464 30.44731882029036 18.96524265813714 26-27 20.200166435954685 30.37241905268278 31.767660949996205 17.65975356136633 28-29 21.596341683563043 30.785245642299913 30.526744860231688 17.091667813905353 30-31 20.346890557999732 33.43366538594866 29.454884753823045 16.76455930222857 32-33 20.35263644029201 31.609519633396303 31.0679637808503 16.969880145461385 34-35 21.6627963433768 32.40128687046627 28.9523146791256 16.983602107031327 36-37 20.95069910921574 33.42500488978404 28.63442460568839 16.989871395311827 38-39 20.855067850117916 30.444980983684378 31.241695246602685 17.458255919595025 40-41 21.009841220391763 31.92066731327015 28.564666297664598 18.504825168673488 42-43 20.65980455748625 32.06264668426186 28.7446461557199 18.53290260253199 44-45 21.078011899091823 30.3085436228413 30.39606599569174 18.21737848237514 46-47 20.627159321439475 31.043123204303534 29.247749838459274 19.081967635797714 48-49 19.967614372750482 31.733934731521472 30.0846159579407 18.213834937787347 50-51 19.636821969362327 31.110264171018905 31.735293132040937 17.517620727577835 52-53 22.5749680275537 30.22232121178618 28.531642236438472 18.671068524221653 54-55 21.506122040362133 31.079842705007348 29.60211877931988 17.81191647531064 56-57 21.70652688604651 30.553023785935494 30.225089872209608 17.515359455808387 58-59 20.566245551030878 31.562317632346808 29.557879885849875 18.31355693077244 60-61 20.833185161660612 31.426982512129847 28.96862607390259 18.77120625230695 62-63 21.80023039424351 29.875688116418836 30.277940111926387 18.046141377411264 64-65 22.150325520008735 29.50685165628255 29.800487426271605 18.542335397437107 66-67 21.481850664618356 30.808910511468294 28.45188020228566 19.25735862162768 68-69 21.753366748409768 29.118371188497317 30.535139732091526 18.593122331001393 70-71 21.13011171919713 29.485716876847206 30.1805990782686 19.203572325687066 72-73 22.291106443699796 29.761815587372336 29.455743855317372 18.491334113610492 74-75 23.069840167785248 28.635720652668358 30.14008270104854 18.15435647849785 76-77 21.17389510767045 29.770785172445375 29.977957695296436 19.077362024587742 78-79 21.00759066340705 31.12122935911611 29.33259752553095 18.538582451945892 80-81 20.8751223360623 30.013014549758722 30.860303808355848 18.25155930582313 82-83 22.09700723598765 30.207855516124678 28.957719756581657 18.73741749130602 84-85 21.408821766254764 31.666819781691647 28.14604640170873 18.778312050344862 86-87 21.552223984655395 31.258999798046666 29.869767354759542 17.3190088625384 88-89 23.095358964972977 28.242645638533187 30.041523517872896 18.62047187862094 90-91 22.258337686546163 30.028943641266746 28.425039502781058 19.28767916940603 92-93 22.161231418521236 28.809057647404305 29.96923467436642 19.060476259708036 94-95 23.088725691427765 29.862607493417272 27.98707587442046 19.061590940734508 96-97 22.84061474541542 29.79833951026093 29.5359773471574 17.825068397166252 98-99 23.750288076184034 28.846963830137167 29.420602195385026 17.982145898293773 100-101 25.026249119471267 29.109346790052705 28.069210199716487 17.79519389075954 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 109.0 1 148.0 2 264.0 3 425.0 4 543.5 5 617.5 6 620.5 7 627.5 8 711.0 9 791.5 10 911.0 11 1076.0 12 1320.0 13 1662.0 14 2396.0 15 12577.0 16 45542.0 17 51679.0 18 43672.0 19 47365.0 20 45743.5 21 45277.0 22 41102.0 23 50456.0 24 72127.5 25 100059.0 26 126496.5 27 188379.0 28 252012.5 29 333317.0 30 411633.5 31 422257.5 32 510420.0 33 758075.0 34 977919.0 35 959686.5 36 769394.5 37 737937.0 38 792546.0 39 890979.0 40 934906.0 41 782043.0 42 619613.5 43 509897.0 44 446227.0 45 366837.0 46 307698.0 47 275167.0 48 262313.5 49 252789.5 50 234399.5 51 216834.5 52 213065.5 53 212557.5 54 205816.0 55 180579.0 56 154236.5 57 144526.0 58 133606.0 59 107821.5 60 78094.5 61 58155.5 62 41977.5 63 29698.5 64 24630.0 65 20397.0 66 14801.5 67 9020.0 68 4721.0 69 3106.0 70 2356.0 71 2622.5 72 4928.5 73 7585.5 74 7708.0 75 5843.0 76 4615.0 77 6334.5 78 5816.0 79 2267.5 80 672.0 81 213.5 82 82.5 83 45.0 84 31.0 85 24.5 86 22.5 87 18.0 88 15.5 89 19.0 90 17.5 91 12.0 92 11.0 93 10.0 94 10.0 95 7.5 96 4.0 97 5.5 98 7.0 99 12.5 100 60.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01007964716618286 2 0.019646335189048528 3 0.019069256152816683 4 0.017472670819241917 5 0.01887048448478127 6 0.03480427787407161 7 0.0273663702959723 8 0.029373322944200815 9 0.04054942027922418 10-11 0.029373322944200815 12-13 0.07474455917060661 14-15 0.12837764359906498 16-17 0.04697743954391777 18-19 0.04764108043558438 20-21 0.03647139508985249 22-23 0.03830201803256573 24-25 0.050638685429344236 26-27 0.0500519884091752 28-29 0.048923478293877366 30-31 0.05157483586589812 32-33 0.04603487711807242 34-35 0.042620492820367346 36-37 0.04591625531618032 38-39 0.04614388093602732 40-41 0.032608171541744876 42-43 0.024580360948830787 44-45 0.0278408575035407 46-47 0.0289469256563184 48-49 0.03350905603719569 50-51 0.02376603830881474 52-53 0.017120011408211348 54-55 0.0521262669449641 56-57 0.0251542339904169 58-59 0.014644983541705887 60-61 0.018899338436592864 62-63 0.018892926447301398 64-65 0.01703344955277657 66-67 0.011522344756762469 68-69 0.016818647911512496 70-71 0.026600137575642238 72-73 0.019453975510304578 74-75 0.01920390792793745 76-77 0.025314533722703522 78-79 0.018876896474072737 80-81 0.01581837758204397 82-83 0.018963458329507514 84-85 0.018796746607929425 86-87 0.01970404309267171 88-89 0.015722197742671994 90-91 0.018559503004145224 92-93 0.018626828891705603 94-95 0.01978098696416929 96-97 0.020617751566705464 98-99 0.020008612584016296 100-101 0.01846652915941898 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5595784E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.479824991890098 #Duplication Level Percentage of deduplicated Percentage of total 1 76.16603471653636 15.5986706132089 2 9.619920625187016 3.9402858167940815 3 3.971148810441395 2.4398529796377693 4 2.22743294884717 1.8246974789423893 5 1.4761685348285964 1.511583662591224 6 0.9736331230296769 1.196390157957311 7 0.7397517146545155 1.0604989957502569 8 0.5573583066288795 0.9131680462028539 9 0.45648168548568957 0.8413798527674947 >10 3.120986873756139 12.470313229970339 >50 0.3497226500464252 4.989655007888684 >100 0.27058587603498474 11.281472740919854 >500 0.034475380027668846 4.904539772796054 >1k 0.029274243282821848 12.638495923235993 >5k 0.003691952404679303 5.181409583969727 >10k+ 0.0033325588077636184 19.20758613736715 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTAT 196932 1.2627258751467705 No Hit CTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAAT 144927 0.9292703720441371 No Hit CGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCAC 144214 0.9246986236793227 No Hit ATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACT 109764 0.703805592588356 No Hit ATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGA 103153 0.6614159313824813 No Hit CGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGG 75063 0.4813031521852316 No Hit AAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCT 63619 0.407924346733707 No Hit CTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAGATGGAGGCT 60730 0.38940010967066485 No Hit ATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTAT 60220 0.38612999513201773 No Hit CTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTT 55126 0.3534673216812954 No Hit CTTTTATATATTATATTATTTGATACTTAACACAAAAATAAGTACGGATC 54459 0.3491905248238883 No Hit CTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAATA 50710 0.32515197697018633 No Hit TGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGC 46422 0.2976573668883847 No Hit CTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTAT 41375 0.26529605693436126 No Hit TTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGA 40835 0.2618335827169702 No Hit AGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGG 40342 0.25867247199627796 No Hit TTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGA 39969 0.2562807999905616 No Hit CTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAA 38078 0.24415572824040138 No Hit ACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTA 36900 0.23660240485505568 No Hit GACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACT 36658 0.23505070344652118 No Hit CTCAGTTTTATTGAAAGCAGATACCATTTCTTCACACAGGTGAGTTTTCT 35239 0.22595209064193247 No Hit CAGGCCTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTA 34445 0.2208609711445093 No Hit CTGTAATTAGTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGA 33852 0.2170586614946706 No Hit ACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAA 33006 0.21163411855409128 No Hit TATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGA 32407 0.2077933369685038 No Hit CTGTAGTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAA 30053 0.19269951417639536 No Hit CTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATAGAT 28613 0.18346624959668587 No Hit CACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAG 27612 0.17704784831592948 No Hit CATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATG 26257 0.1683596028259945 No Hit GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT 26201 0.16800053142567248 No Hit TTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAG 25905 0.16610258259539887 No Hit GGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCA 25824 0.1655832114627902 No Hit GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT 25214 0.1616718979949966 No Hit GTTGAATTACAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTG 24207 0.15521502477849142 No Hit GACTCACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGA 23785 0.15250916529749323 No Hit TCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCACTATA 23287 0.14931599463034367 No Hit ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT 23100 0.14811695263283975 No Hit GGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTCAC 22958 0.1472064501534517 No Hit CGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACTATA 22732 0.14575734057358064 No Hit ATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGAC 21560 0.13824248912398376 No Hit CAACGATGATTTTTCATGTCATTGGTCGCAGTTGAATGCTGTGTAGAAAT 21510 0.13792188965941052 No Hit CACTATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATAC 20933 0.13422217183823526 No Hit AGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATA 19246 0.12340514590353394 No Hit ATACGACTCACTATAGATAATACGACTCACTATAGATAATACGACTCACT 19126 0.12263570718855814 No Hit CTTTAATGAAATGACTGTGGAAATAACATATATTTCCATGACACCAGTAC 18807 0.12059028260458084 No Hit GAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGT 18675 0.11974390001810746 No Hit TAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGACTC 18596 0.11923735286408174 No Hit ACGACTCACTATAGAGATAATACGACTCACTATAGAGATAATACGACTCA 18324 0.11749329177680326 No Hit ATTTTAGTATTTTGTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAG 17958 0.1151465036961271 No Hit CAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAA 17922 0.11491567208163436 No Hit AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTA 17792 0.11408211347374393 No Hit GTTCATTCTAATCCTTTTTGTGTTCATTCATATGCTAGGCCTAGAGATAG 17747 0.113793573955628 No Hit GTTAAACCACACAATGGATCTGTGAACTTGTGAGCTTGGGTATTACACAT 17463 0.11197256899685196 No Hit CGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGTATGAG 17173 0.11011309210232714 No Hit GTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCT 17149 0.10995920435933199 No Hit TTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTA 17058 0.10937571333380867 No Hit TATAGGAATACGACTCACTATAGGAATACGACTCACTATAGGAATACGAC 16409 0.10521433228364793 No Hit GTCTTCGATAATTCCTGAGATTGGTATAAGAATTAAGATGATTGAGAAGT 16273 0.10434230174000872 No Hit AGAGATAATACGACTCACTATAGAGATAATACGACTCACTATAGAGATAA 16232 0.10407941017905864 No Hit CCCAATTCAGGTTAAGATAAGTAGGTTGGCTACTAGGATTCAGTACAAAA 16142 0.10350233114282681 No Hit CTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAGCTCGTTAGGCTTT 15814 0.10139919865522631 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.1295914331719396E-5 0.0 0.0 0.0 0.0 2 5.7707903623184316E-5 0.0 0.0 6.4119892914649245E-6 0.0 3 5.7707903623184316E-5 0.0 0.0 6.4119892914649245E-6 0.0 4 7.053188220611417E-5 0.0 0.0 6.4119892914649245E-6 0.0 5 7.053188220611417E-5 0.0 0.0 6.4119892914649245E-6 0.0 6 7.694387149757908E-5 0.0 0.0 6.4119892914649245E-6 0.0 7 8.335586078904401E-5 0.0 0.0 6.4119892914649245E-6 0.0 8 8.335586078904401E-5 0.0 0.0 6.4119892914649245E-6 0.0 9 8.976785008050894E-5 0.0 0.0 6.4119892914649245E-6 0.0 10-11 1.0259182866343878E-4 0.0 0.0 6.4119892914649245E-6 0.0 12-13 1.3144578047503096E-4 0.0 0.0 6.4119892914649245E-6 0.0 14-15 1.763297055152854E-4 0.0 0.0 6.4119892914649245E-6 0.0 16-17 2.115956466183425E-4 0.0 0.0 6.4119892914649245E-6 0.0 18-19 2.372436037842022E-4 0.0 0.0 6.4119892914649245E-6 0.0 20-21 3.270114538647111E-4 0.0 0.0 6.4119892914649245E-6 0.0 22-23 4.5845723433974206E-4 0.0 0.0 6.4119892914649245E-6 0.0 24-25 6.540229077294222E-4 0.0 0.0 6.4119892914649245E-6 0.0 26-27 9.71416377656936E-4 0.0 0.0 6.4119892914649245E-6 0.0 28-29 0.0015324654406601167 0.0 0.0 6.4119892914649245E-6 6.4119892914649245E-6 30-31 0.0025327357701286453 0.0 0.0 6.4119892914649245E-6 6.4119892914649245E-6 32-33 0.00447877452008825 0.0 0.0 1.2823978582929849E-5 6.4119892914649245E-6 34-35 0.0072872258297498865 0.0 0.0 1.2823978582929849E-5 6.4119892914649245E-6 36-37 0.011438988895973425 6.4119892914649245E-6 0.0 1.2823978582929849E-5 6.4119892914649245E-6 38-39 0.01773235638554625 6.4119892914649245E-6 0.0 1.2823978582929849E-5 6.4119892914649245E-6 40-41 0.025516511385384664 6.4119892914649245E-6 0.0 1.2823978582929849E-5 6.4119892914649245E-6 42-43 0.03566669043377364 6.4119892914649245E-6 0.0 1.923596787439477E-5 6.4119892914649245E-6 44-45 0.04870226466332183 6.4119892914649245E-6 0.0 1.923596787439477E-5 6.4119892914649245E-6 46-47 0.06506245534049458 6.4119892914649245E-6 0.0 1.923596787439477E-5 6.4119892914649245E-6 48-49 0.08514480580136273 6.4119892914649245E-6 0.0 1.923596787439477E-5 6.4119892914649245E-6 50-51 0.10832735308465416 6.4119892914649245E-6 0.0 1.923596787439477E-5 6.4119892914649245E-6 52-53 0.13956977090731698 6.4119892914649245E-6 0.0 1.923596787439477E-5 6.4119892914649245E-6 54-55 0.18035322879567967 6.4119892914649245E-6 0.0 1.923596787439477E-5 6.4119892914649245E-6 56-57 0.22711266070368763 6.4119892914649245E-6 0.0 1.923596787439477E-5 6.4119892914649245E-6 58-59 0.2794409053113328 6.4119892914649245E-6 0.0 1.923596787439477E-5 6.4119892914649245E-6 60-61 0.33882875012888103 6.4119892914649245E-6 0.0 1.923596787439477E-5 6.4119892914649245E-6 62-63 0.41197351797126713 6.4119892914649245E-6 0.0 1.923596787439477E-5 6.4119892914649245E-6 64-65 0.49624949922363637 6.4119892914649245E-6 0.0 1.923596787439477E-5 6.4119892914649245E-6 66-67 0.59113732275338 6.4119892914649245E-6 0.0 1.923596787439477E-5 6.4119892914649245E-6 68-69 0.701192706952084 6.4119892914649245E-6 0.0 1.923596787439477E-5 9.617983937197387E-6 70-71 0.8196189431707954 6.4119892914649245E-6 0.0 1.923596787439477E-5 1.2823978582929849E-5 72-73 0.9601344824986034 6.4119892914649245E-6 0.0 1.923596787439477E-5 1.2823978582929849E-5 74-75 1.1378235297436794 6.4119892914649245E-6 0.0 1.923596787439477E-5 1.2823978582929849E-5 76-77 1.3347357208845674 6.4119892914649245E-6 0.0 1.923596787439477E-5 1.2823978582929849E-5 78-79 1.5575395247843904 6.4119892914649245E-6 0.0 1.923596787439477E-5 1.2823978582929849E-5 80-81 1.810213580798503 6.4119892914649245E-6 0.0 1.923596787439477E-5 1.2823978582929849E-5 82-83 2.101846883747556 6.4119892914649245E-6 0.0 1.923596787439477E-5 1.923596787439477E-5 84-85 2.4702958184083594 6.4119892914649245E-6 0.0 1.923596787439477E-5 1.923596787439477E-5 86-87 2.8686374471459724 6.4119892914649245E-6 0.0 1.923596787439477E-5 1.923596787439477E-5 88-89 3.299555187478872 6.4119892914649245E-6 0.0 1.923596787439477E-5 1.923596787439477E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTGT 9360 0.0 80.36692 1 GCGTGTT 11500 0.0 63.099754 2 GATCGGG 1200 0.0 53.82148 1 CGTGTTT 14520 0.0 49.323307 3 TCGAACG 815 0.0 48.946747 2 CGAACGT 1580 0.0 48.39124 1 ATCGGGA 1440 0.0 44.521873 2 GAGCGGC 1685 0.0 43.23477 9 GTAGATA 14165 0.0 39.024605 2 TAGATAT 15255 0.0 36.393158 3 CTTACGG 3290 0.0 35.653244 1 GATATAG 16150 0.0 35.377853 5 TTACGGT 3370 0.0 34.38445 2 AGATATA 16980 0.0 33.81539 4 GTAAACG 1415 0.0 33.561512 1 GTCGAAC 1230 0.0 33.204067 1 GCCGGAT 1395 0.0 33.02193 46-47 TCGGGGG 2740 0.0 32.848045 38-39 CGCCGGA 1090 0.0 32.023277 46-47 GTGTTTT 22215 0.0 31.89689 4 >>END_MODULE