FastQCFastQC Report
Fri 27 May 2016
SRR1233121_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1233121_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15595784
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAG1326700.8506786192986514No Hit
CTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTG1136520.7287354069535716No Hit
GTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATT1004760.6442510360492297No Hit
GTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATA998530.6402563667206471No Hit
CTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC937720.6012650598392488No Hit
TATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTA868830.557092865610347No Hit
CCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCG753140.4829125614973893No Hit
CAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTA700330.449050846049163No Hit
CTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGT698550.44790951195528234No Hit
CCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCT626780.40189066481043856No Hit
CTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATT577860.3705232131965921No Hit
ATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTC540590.3466257291073023No Hit
CGTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGT500650.3210162438771914No Hit
CTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCC497510.3190028792396714No Hit
GTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATC463880.2974393592524749No Hit
CACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGA449130.2879816750475641No Hit
CTGGATTCCATGGACTCCATGTAATTATTGGATCAACATTCCTTATTGTT444470.2849936880377415No Hit
CTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTC432900.27757501642751653No Hit
CTTCAATCAATCTAATAGGAGAATTATTCATTACCATATCATTATTTTCT382170.24504699475191502No Hit
GCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATC370900.23782068282043406No Hit
TCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGT364340.233614417845233No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCTA317870.20381790360779556No Hit
CTCACACACACGAGAACTAACACTAATAGCCCTTCACATAATTCCACTTA307790.1973546184019989No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCG299190.19184030761133908No Hit
GTATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTG298020.19109010486423766No Hit
CCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAG298000.19107728088565473No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC283180.18157471275570372No Hit
CCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATT277730.17808017859185535No Hit
GTCGTATTATCTATAGTGAGTCGTATTATCTATAGTGAGTCGTATTATCT275640.17674007282993917No Hit
CCCGAAACCAAACGAGCTACCTAAAAACAATTTTATGAATCAACTCGTCT265520.17025113966697666No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC262970.1686160823976531TruSeq Adapter, Index 3 (100% over 50bp)
AATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAA259310.16626929431697693No Hit
CTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCAATCACA259220.16621158641335376No Hit
ATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTAT256870.1647047689298595No Hit
ATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGAG248490.1593315219036119No Hit
CCAAACCCTAAAACCATTAAACAACCAACAAACCCACTAACAATTAAACC224440.14391068765763876No Hit
GTGCTTTCCAAAAGGTTGCCGCCACTTCCCGTTAAGATAATTAACTTTTG214450.13750511035546528No Hit
CTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAAC212210.13606882475417714No Hit
TAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCA208190.13349120505900827No Hit
CTGATATTATGTACACGGGGACACTTGACTGCTGGAGGAAGATTGCAAAA207730.13319625355160086No Hit
CAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTCCAAAAGGT198120.1270343318425031No Hit
CGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGC194610.1247837236011989No Hit
TATTCCTATAGTGAGTCGTATTCCTATAGTGAGTCGTATTCCTATAGTGA190770.12232151971327636No Hit
CTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTA184470.11828196645965346No Hit
CGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCGTATTAT183030.11735864000168252No Hit
CTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATTGGA180400.11567228681802723No Hit
GTGAGTCGTATTATCTCTATAGTGAGTCGTATTATCTCTATAGTGAGTCG180310.11561457891440403No Hit
ATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACT179840.1153132154177052No Hit
CAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACA178350.11435782901327693No Hit
GGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAA177410.11375510201987922No Hit
CCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTA176820.1133767946516828No Hit
ATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAG173600.11131213409983108No Hit
CCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTAT171330.10985661253066854No Hit
CACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCT165760.10628513449532258No Hit
CACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGG165670.1062274265916994No Hit
CGGCAATCTTGATTATAAGAATTTGGTCCACATCATTACCCACGGAGAAG164140.10524639223010526No Hit
CGCTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTT163880.10507968050852717No Hit
CTATATTCATAGCACTTATACTAAATAACTCTATAACCATCAACTCAATC163660.10493861674411495No Hit
CTGGTGTCCAAGGTTCCAAGGTTTCCAAGCAACAAGCTAAGGGTGCTTTC162860.10442565760079775No Hit
TCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCA158040.10133507876231165No Hit
AATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCAA156590.10040534031504926No Hit
CTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACA156580.10039892832575778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAA67400.044.0500031
CGGCAAT45000.041.4471631
TACGCAT47550.039.650389
CTACGCA48700.038.421288
CTCTACG48850.038.0296976
CTCCGCT15300.037.6283531
TCCGCTC13900.036.9172552
CCCGATC6350.035.965721
CCGCAAA29400.033.4999581
TCGTACC28050.033.3698162
ACTCGTC96050.033.33053242-43
GGCAATC57400.032.7796062
CTCGTAC29700.032.520771
TCGTCTA101550.031.64227144-45
CTAACGA95550.031.58693994-95
CATGGAC161900.031.1519369
CGTACCA29950.030.9355753
CGCAAAC31850.030.7311062
CCGAAAC119550.030.6053961
TCCACTC336300.030.1641752
Produced by FastQC (version 0.11.5)